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The map label for this gene is ruvC [H]

Identifier: 161511192

GI number: 161511192

Start: 2057741

End: 2058307

Strand: Reverse

Name: ruvC [H]

Synonym: BT_1664

Alternate gene names: 161511192

Gene position: 2058307-2057741 (Counterclockwise)

Preceding gene: 29347075

Following gene: 29347073

Centisome position: 32.88

GC content: 45.15

Gene sequence:

>567_bases
GTGATTCAACCGGTAAAAGAAAAGATTATTTTGGGTATAGACCCCGGTACGACCATCATGGGATATGGAGTCTTGCGCGT
GAAAGGGACTAAGCCTGAGATGATCGCGATGGGAATTATCGATTTACGTAAATTTGCCAATCATTATCTGAAGCTGCGGC
ATATTCACGAACGGGTATTAAGTATCATCGAAAGCTATCTGCCTGACGAACTGGCTATCGAAGCTCCTTTTTTCGGCAAG
AACGTACAGTCCATGTTGAAGTTGGGACGTGCTCAGGGAGTGGCGATGGCAGTGGCATTGAGCCGTGATATTCCGATCAC
AGAATATGCTCCTTTGAAGATCAAGATGGCTATAACCGGAAACGGACAGGCATCAAAGGAACAAGTAGCGGATATGCTGC
AGCGTATGCTGCATTTTCCCAAAGAAGAGATGCCTACCTTTATGGATGCGACGGACGGTTTGGCGGCAGCCTATTGTCAT
TTTCTGCAAATGGGACGTCCGGCAATGGAAAAAGGATATAGCAGTTGGAAAGATTTTATTGCGAAAAATCCCGATAAAGT
AAAATAA

Upstream 100 bases:

>100_bases
ATCGCTGGCACATGGAACAGGGAGTGCGGCTGAATGACGAACTTACGAAAATATTCAAGGATAAAGTAGTCGGTTTTCCG
ACATTGATGGAGATAGTAGA

Downstream 100 bases:

>100_bases
ACCCGCAATGAAAGAGGCTGGGAGTGAGAAAAGTAATAAACAAGTAAGTGCGAAGGTAGTGTGAGTACCGCAGGGGAGGT
TTAGTATGAAAATGGGAACC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MIQPVKEKIILGIDPGTTIMGYGVLRVKGTKPEMIAMGIIDLRKFANHYLKLRHIHERVLSIIESYLPDELAIEAPFFGK
NVQSMLKLGRAQGVAMAVALSRDIPITEYAPLKIKMAITGNGQASKEQVADMLQRMLHFPKEEMPTFMDATDGLAAAYCH
FLQMGRPAMEKGYSSWKDFIAKNPDKVK

Sequences:

>Translated_188_residues
MIQPVKEKIILGIDPGTTIMGYGVLRVKGTKPEMIAMGIIDLRKFANHYLKLRHIHERVLSIIESYLPDELAIEAPFFGK
NVQSMLKLGRAQGVAMAVALSRDIPITEYAPLKIKMAITGNGQASKEQVADMLQRMLHFPKEEMPTFMDATDGLAAAYCH
FLQMGRPAMEKGYSSWKDFIAKNPDKVK
>Mature_188_residues
MIQPVKEKIILGIDPGTTIMGYGVLRVKGTKPEMIAMGIIDLRKFANHYLKLRHIHERVLSIIESYLPDELAIEAPFFGK
NVQSMLKLGRAQGVAMAVALSRDIPITEYAPLKIKMAITGNGQASKEQVADMLQRMLHFPKEEMPTFMDATDGLAAAYCH
FLQMGRPAMEKGYSSWKDFIAKNPDKVK

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=132, Percent_Identity=40.9090909090909, Blast_Score=101, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 21036; Mature: 21036

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
6.9 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
6.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQPVKEKIILGIDPGTTIMGYGVLRVKGTKPEMIAMGIIDLRKFANHYLKLRHIHERVL
CCCCCCCCEEEECCCCCEEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIIESYLPDELAIEAPFFGKNVQSMLKLGRAQGVAMAVALSRDIPITEYAPLKIKMAITG
HHHHHHCCCHHEEECCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCCCEEEEEEEEC
NGQASKEQVADMLQRMLHFPKEEMPTFMDATDGLAAAYCHFLQMGRPAMEKGYSSWKDFI
CCCCCHHHHHHHHHHHHHCCHHHCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AKNPDKVK
HCCCCCCC
>Mature Secondary Structure
MIQPVKEKIILGIDPGTTIMGYGVLRVKGTKPEMIAMGIIDLRKFANHYLKLRHIHERVL
CCCCCCCCEEEECCCCCEEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIIESYLPDELAIEAPFFGKNVQSMLKLGRAQGVAMAVALSRDIPITEYAPLKIKMAITG
HHHHHHCCCHHEEECCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCCCEEEEEEEEC
NGQASKEQVADMLQRMLHFPKEEMPTFMDATDGLAAAYCHFLQMGRPAMEKGYSSWKDFI
CCCCCHHHHHHHHHHHHHCCHHHCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AKNPDKVK
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA