Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 29346774

GI number: 29346774

Start: 1692056

End: 1693180

Strand: Reverse

Name: dnaN [H]

Synonym: BT_1364

Alternate gene names: 29346774

Gene position: 1693180-1692056 (Counterclockwise)

Preceding gene: 29346776

Following gene: 29346773

Centisome position: 27.05

GC content: 47.64

Gene sequence:

>1125_bases
ATGAAATTTATCGTTTCAAGTACTGCTCTTTCCAGCCATTTACAGGCTATCAGTCGTGTAATTAACTCAAAGAATGCGCT
GCCTATTCTCGATTGTTTTCTCTTCGAATTGGAAGACGGAACATTGTCTGTGACTGTTTCTGACAGTGAAACGACCATGG
TGACAACCGTTGAGGTAAATGAAAGCGATACAAACGGGCGTTTTGCCGTAGTAGCGAAAACATTGCTTGATGCACTGAAA
GAAATTCCGGAACAACCGTTGACATTTTACGTCAACACGGACAATTATGAAATCACCGTGCAGTATCAGAACGGAAAATA
TAGTCTGATGGGACAGAATGCTGACGAATTTCCGCAGTCGGCTGTGCTGGGCGAAAATGCAGTCCGCGTGGAGATGGAAG
CAGGTGTACTGCTGGGTGGCATCAACCGTTCGGTATTCGCAACTGCCGACGACGAACTCCGTCCGGTAATGAACGGTATA
TATTTCGATATTACAACAGAAGACATCACGATGGTGGCATCGGACGGTCATAAGCTGGTACGTTGCAAAACGCTGGCTGC
CAAAGGAAACGAACGTGCCGCGTTCATCCTTCCGAAGAAGCCCGCTACATTGCTGAAAAACCTGCTTCCGAAAGAACAGG
GAACAGTAACCATCGAATTTGACGAACGCAACGCTGTGTTTATGCTGGAAAGCTACCGCATGGTCTGCCGTCTGATCGAA
GGACGCTATCCGAACTACAACTCGGTAATTCCGCAGAACAACCCTCATAAAGTGACTGTAGACCGTCAGCAACTGATCGG
AGCTTTGCGCCGCGTGTCTATCTTCTCTTCGCAGGCAAGCAGTTTGATCAAACTGCGTATGCAGGAGAATCAGATTGTGA
TTTCCGCACAGGACATCGATTTCTCCACTTCTGCCGAAGAAACACAAGTTTGCCAATATTCGGGTGCCGCTATGAGCATC
GGATTCAAGTCTACTTTCCTCATCGATATCCTGAACAATATTTCGGCCGATGAAGTGGTAATCGAGCTTGCCGACCCGTC
GCGTGCCGGAGTCATTGTGCCGGTAGAACAGGAAGAAAATGAAGACTTGCTGATGCTGCTGATGCCGATGATGCTGAACG
ATTAA

Upstream 100 bases:

>100_bases
CACACAATGCTTGTTTTTTCTTTCGTTTTCTTGGGCAGCACGAATCTTTTTTCGTAACTTTGTGCGATATATAATTCAAA
ACTTAAATACTCGAAAATAT

Downstream 100 bases:

>100_bases
TTAATTATTTAATGGATAATTGAGAATGGATAATGGATAATGAGCTGCGCTGTCAGTGGTCAGGAGTTATCCATTTTTTA
TGTTTCCTATCCATTATCAA

Product: DNA polymerase III subnit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKFIVSSTALSSHLQAISRVINSKNALPILDCFLFELEDGTLSVTVSDSETTMVTTVEVNESDTNGRFAVVAKTLLDALK
EIPEQPLTFYVNTDNYEITVQYQNGKYSLMGQNADEFPQSAVLGENAVRVEMEAGVLLGGINRSVFATADDELRPVMNGI
YFDITTEDITMVASDGHKLVRCKTLAAKGNERAAFILPKKPATLLKNLLPKEQGTVTIEFDERNAVFMLESYRMVCRLIE
GRYPNYNSVIPQNNPHKVTVDRQQLIGALRRVSIFSSQASSLIKLRMQENQIVISAQDIDFSTSAEETQVCQYSGAAMSI
GFKSTFLIDILNNISADEVVIELADPSRAGVIVPVEQEENEDLLMLLMPMMLND

Sequences:

>Translated_374_residues
MKFIVSSTALSSHLQAISRVINSKNALPILDCFLFELEDGTLSVTVSDSETTMVTTVEVNESDTNGRFAVVAKTLLDALK
EIPEQPLTFYVNTDNYEITVQYQNGKYSLMGQNADEFPQSAVLGENAVRVEMEAGVLLGGINRSVFATADDELRPVMNGI
YFDITTEDITMVASDGHKLVRCKTLAAKGNERAAFILPKKPATLLKNLLPKEQGTVTIEFDERNAVFMLESYRMVCRLIE
GRYPNYNSVIPQNNPHKVTVDRQQLIGALRRVSIFSSQASSLIKLRMQENQIVISAQDIDFSTSAEETQVCQYSGAAMSI
GFKSTFLIDILNNISADEVVIELADPSRAGVIVPVEQEENEDLLMLLMPMMLND
>Mature_374_residues
MKFIVSSTALSSHLQAISRVINSKNALPILDCFLFELEDGTLSVTVSDSETTMVTTVEVNESDTNGRFAVVAKTLLDALK
EIPEQPLTFYVNTDNYEITVQYQNGKYSLMGQNADEFPQSAVLGENAVRVEMEAGVLLGGINRSVFATADDELRPVMNGI
YFDITTEDITMVASDGHKLVRCKTLAAKGNERAAFILPKKPATLLKNLLPKEQGTVTIEFDERNAVFMLESYRMVCRLIE
GRYPNYNSVIPQNNPHKVTVDRQQLIGALRRVSIFSSQASSLIKLRMQENQIVISAQDIDFSTSAEETQVCQYSGAAMSI
GFKSTFLIDILNNISADEVVIELADPSRAGVIVPVEQEENEDLLMLLMPMMLND

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=372, Percent_Identity=27.1505376344086, Blast_Score=145, Evalue=5e-36,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41413; Mature: 41413

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIVSSTALSSHLQAISRVINSKNALPILDCFLFELEDGTLSVTVSDSETTMVTTVEVN
CEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEECCCEEEEEEEEEC
ESDTNGRFAVVAKTLLDALKEIPEQPLTFYVNTDNYEITVQYQNGKYSLMGQNADEFPQS
CCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEECCCEEEEECCCHHHCCCH
AVLGENAVRVEMEAGVLLGGINRSVFATADDELRPVMNGIYFDITTEDITMVASDGHKLV
HHCCCCEEEEEEECCEEEECCCCEEEECCCHHHHHHHCCEEEEEECCCEEEEECCCCEEE
RCKTLAAKGNERAAFILPKKPATLLKNLLPKEQGTVTIEFDERNAVFMLESYRMVCRLIE
EEEEEECCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHH
GRYPNYNSVIPQNNPHKVTVDRQQLIGALRRVSIFSSQASSLIKLRMQENQIVISAQDID
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEEEECCC
FSTSAEETQVCQYSGAAMSIGFKSTFLIDILNNISADEVVIELADPSRAGVIVPVEQEEN
CCCCCCHHHHEEECCCEEEECCCCEEHHHHHHCCCCCCEEEEECCCCCCCEEEEECCCCC
EDLLMLLMPMMLND
CCHHHHHHHHHHCC
>Mature Secondary Structure
MKFIVSSTALSSHLQAISRVINSKNALPILDCFLFELEDGTLSVTVSDSETTMVTTVEVN
CEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEECCCEEEEEEEEEC
ESDTNGRFAVVAKTLLDALKEIPEQPLTFYVNTDNYEITVQYQNGKYSLMGQNADEFPQS
CCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEECCCEEEEECCCHHHCCCH
AVLGENAVRVEMEAGVLLGGINRSVFATADDELRPVMNGIYFDITTEDITMVASDGHKLV
HHCCCCEEEEEEECCEEEECCCCEEEECCCHHHHHHHCCEEEEEECCCEEEEECCCCEEE
RCKTLAAKGNERAAFILPKKPATLLKNLLPKEQGTVTIEFDERNAVFMLESYRMVCRLIE
EEEEEECCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHH
GRYPNYNSVIPQNNPHKVTVDRQQLIGALRRVSIFSSQASSLIKLRMQENQIVISAQDID
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEEEECCC
FSTSAEETQVCQYSGAAMSIGFKSTFLIDILNNISADEVVIELADPSRAGVIVPVEQEEN
CCCCCCHHHHEEECCCEEEECCCCEEHHHHHHCCCCCCEEEEECCCCCCCEEEEECCCCC
EDLLMLLMPMMLND
CCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]