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The map label for this gene is ycjG [C]

Identifier: 29346723

GI number: 29346723

Start: 1637748

End: 1638899

Strand: Reverse

Name: ycjG [C]

Synonym: BT_1313

Alternate gene names: 29346723

Gene position: 1638899-1637748 (Counterclockwise)

Preceding gene: 29346724

Following gene: 29346722

Centisome position: 26.18

GC content: 45.92

Gene sequence:

>1152_bases
ATGCCAAATAGAAGAGATTTTCTGAAAACGGCTGCCTTTGCGACTTTAGGTTCCGGAATAGCTGTCAGTCAGGTATTGGC
GGGAGAATGTATGCCTTCTGCTATTCATATTAATAAATATGGTATAGGGGGAAAGATGAAAATGACTTTTTTCCCTTATG
AGTTAAAGTTGAGGCATGTCTTTACGGTAGCCACTTATTCGCGTACTACTACGCCCGATGTGCAGGTAGAGATCGAATAT
GAAGGGGTGACCGGGTATGGAGAAGCGTCTATGCCCCCTTATCTCGGAGAAACAGTGGAGTCGGTAATGAACTTCCTTAA
AAAAGTAAATCTGGAGCAATTCAGCGATCCTTTTCAATTAGAGGACATTTTGTCATACGTTGACAGTCTGTCGCCTAAAG
ACACGGCAGCAAAAGCTGCGGTGGATATTGCCCTTCATGACTTGGTGGGAAAGCTGCTGGGAGCGCCTTGGTATAAGATA
TGGGGATTGAACAAAGAGAAGACGCCTTCTACCACCTTTACGATTGGTATTGATACGCCCGATGTGGTCCGTGCGAAAAC
AAAAGAATGTGCCGGTCTGTTTAATATATTAAAGGTGAAGCTGGGAAGAGACAATGATAAGGAAATGATAGAGACAATCC
GTTCGGTAACGGACCTGCCGATAGCCGTTGATGCCAACCAGGGCTGGAAAGACCGCCAATATGCACTCGATATGATTCAT
TGGCTGAAAGAGAAAGGGATTGTGATGATAGAGCAGCCTATGCCGAAAGAGCAGTTGGACGACATTGCTTGGGTGACGCA
ACAAAGTCCTTTGCCTGTATTTGCCGACGAGTCGTTGCAGCGTTTAGGGGATGTAGCCGCTTTGAAAGGTGCATTTACGG
GAATCAATATTAAATTGATGAAATGTACCGGAATGCGTGAAGCATGGAAGATGGTCACGCTGGCACATGCTTTGGGAATG
CGCGTGATGGTGGGGTGTATGACGGAGACTTCGTGTGCGATATCTGCTGCCTCGCAATTTTCTCCGGCAGTCGATTTTGC
CGACCTTGATGGAAACCTGCTTATCTCCAACGACCGCTTCAAAGGAGTGGAAGTAGTGAATGGAAAAATCACATTGAATG
ACTTACCCGGAATTGGTGTCATGAAAATCTGA

Upstream 100 bases:

>100_bases
TGCCACTCGCTTCCTGCCCTATATAAATAAGGAGAAAGGTATGAATACCACCGACCATAACCTCTATTATCTACATCACT
AACCATTATCCATTAAACAA

Downstream 100 bases:

>100_bases
TCTTACTGAATGAATGTCAGACTGTTAAAGCCATCTGACATACAACGAATGTAAATTTGTCAGTTGCTGTCGTTAAGCGC
TGTTAAGCCCTAAATATTCT

Product: muconate cycloisomerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPNRRDFLKTAAFATLGSGIAVSQVLAGECMPSAIHINKYGIGGKMKMTFFPYELKLRHVFTVATYSRTTTPDVQVEIEY
EGVTGYGEASMPPYLGETVESVMNFLKKVNLEQFSDPFQLEDILSYVDSLSPKDTAAKAAVDIALHDLVGKLLGAPWYKI
WGLNKEKTPSTTFTIGIDTPDVVRAKTKECAGLFNILKVKLGRDNDKEMIETIRSVTDLPIAVDANQGWKDRQYALDMIH
WLKEKGIVMIEQPMPKEQLDDIAWVTQQSPLPVFADESLQRLGDVAALKGAFTGINIKLMKCTGMREAWKMVTLAHALGM
RVMVGCMTETSCAISAASQFSPAVDFADLDGNLLISNDRFKGVEVVNGKITLNDLPGIGVMKI

Sequences:

>Translated_383_residues
MPNRRDFLKTAAFATLGSGIAVSQVLAGECMPSAIHINKYGIGGKMKMTFFPYELKLRHVFTVATYSRTTTPDVQVEIEY
EGVTGYGEASMPPYLGETVESVMNFLKKVNLEQFSDPFQLEDILSYVDSLSPKDTAAKAAVDIALHDLVGKLLGAPWYKI
WGLNKEKTPSTTFTIGIDTPDVVRAKTKECAGLFNILKVKLGRDNDKEMIETIRSVTDLPIAVDANQGWKDRQYALDMIH
WLKEKGIVMIEQPMPKEQLDDIAWVTQQSPLPVFADESLQRLGDVAALKGAFTGINIKLMKCTGMREAWKMVTLAHALGM
RVMVGCMTETSCAISAASQFSPAVDFADLDGNLLISNDRFKGVEVVNGKITLNDLPGIGVMKI
>Mature_382_residues
PNRRDFLKTAAFATLGSGIAVSQVLAGECMPSAIHINKYGIGGKMKMTFFPYELKLRHVFTVATYSRTTTPDVQVEIEYE
GVTGYGEASMPPYLGETVESVMNFLKKVNLEQFSDPFQLEDILSYVDSLSPKDTAAKAAVDIALHDLVGKLLGAPWYKIW
GLNKEKTPSTTFTIGIDTPDVVRAKTKECAGLFNILKVKLGRDNDKEMIETIRSVTDLPIAVDANQGWKDRQYALDMIHW
LKEKGIVMIEQPMPKEQLDDIAWVTQQSPLPVFADESLQRLGDVAALKGAFTGINIKLMKCTGMREAWKMVTLAHALGMR
VMVGCMTETSCAISAASQFSPAVDFADLDGNLLISNDRFKGVEVVNGKITLNDLPGIGVMKI

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=327, Percent_Identity=36.085626911315, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 42168; Mature: 42037

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNRRDFLKTAAFATLGSGIAVSQVLAGECMPSAIHINKYGIGGKMKMTFFPYELKLRHV
CCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECEEEEEEEE
FTVATYSRTTTPDVQVEIEYEGVTGYGEASMPPYLGETVESVMNFLKKVNLEQFSDPFQL
EEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCH
EDILSYVDSLSPKDTAAKAAVDIALHDLVGKLLGAPWYKIWGLNKEKTPSTTFTIGIDTP
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCC
DVVRAKTKECAGLFNILKVKLGRDNDKEMIETIRSVTDLPIAVDANQGWKDRQYALDMIH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHH
WLKEKGIVMIEQPMPKEQLDDIAWVTQQSPLPVFADESLQRLGDVAALKGAFTGINIKLM
HHHHCCEEEEECCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEE
KCTGMREAWKMVTLAHALGMRVMVGCMTETSCAISAASQFSPAVDFADLDGNLLISNDRF
EECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCEEEECCCC
KGVEVVNGKITLNDLPGIGVMKI
CCEEEEECEEEEECCCCCCEECC
>Mature Secondary Structure 
PNRRDFLKTAAFATLGSGIAVSQVLAGECMPSAIHINKYGIGGKMKMTFFPYELKLRHV
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECEEEEEEEE
FTVATYSRTTTPDVQVEIEYEGVTGYGEASMPPYLGETVESVMNFLKKVNLEQFSDPFQL
EEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCH
EDILSYVDSLSPKDTAAKAAVDIALHDLVGKLLGAPWYKIWGLNKEKTPSTTFTIGIDTP
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCC
DVVRAKTKECAGLFNILKVKLGRDNDKEMIETIRSVTDLPIAVDANQGWKDRQYALDMIH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHH
WLKEKGIVMIEQPMPKEQLDDIAWVTQQSPLPVFADESLQRLGDVAALKGAFTGINIKLM
HHHHCCEEEEECCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEE
KCTGMREAWKMVTLAHALGMRVMVGCMTETSCAISAASQFSPAVDFADLDGNLLISNDRF
EECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCEEEECCCC
KGVEVVNGKITLNDLPGIGVMKI
CCEEEEECEEEEECCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]