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The map label for this gene is lacZ [H]

Identifier: 29346393

GI number: 29346393

Start: 1205904

End: 1208552

Strand: Reverse

Name: lacZ [H]

Synonym: BT_0983

Alternate gene names: 29346393

Gene position: 1208552-1205904 (Counterclockwise)

Preceding gene: 29346394

Following gene: 29346392

Centisome position: 19.3

GC content: 48.4

Gene sequence:

>2649_bases
ATGAATAAAAGACCGTTTTTAATCTTATCCCTATTTCTACTGCTATTCTCCTTCGATGGGCAGGCAGCAGATACTTATCG
TCCCGAAATATCGGTTGCCGGATTTATTCCGCTTCCCGATTGCGGGCGACAAGTATATAACTTCAATCCGGGATGGCGAT
TCTTCAAAGGCGATATCCGGGGGGCGGAAGCTGTAAATTTTGACGATCGTTCTTGGGAGGTCGTTTCTACTCCTCACACA
GTGGAACTGATGCCCGCCGAAGGCAGTGGTTGCCGCAATTATCAGGGTCCTGCGTGGTATCGCAAGCATTTTGTAGTTCC
CACTGCAACGAAAGGACAGCGTGTACTCCTTCATTTTGAAGCGGCAATGGGAAAGCAAGTACTTTACCTGAACGGCAAAC
GTGTCCAGGAACATCTTGGAGGTTATCTGCCTTTCACACTGGACTTGACGGCAAACGGCGTACAAGCCGGAGACTCCTGC
CTCCTTGCCGTCTATACCGACAACAGCAACGATAAATCATACCCTCCCGGAAAGCCCCAATACACACTGGATTTTGCTTA
TCATGGAGGTATCTATCGTGATGTATGGATGATTGCCAAATCTCCGGTTGCCATTACCGATGCCATCGACTCCCGCACAG
TTGGCGGTGGCGGCGTATTCGTTCACTTTGACAAGATCAGCGAAAAGAGCGCACAAGTATATGTAGAAACGGAGATTCAG
AACGATAATACCCGTTCGGAATCCGTCACTATAGAAACGACCCTTACAGATGCCGAAGGCAACGTTATCAAACGTACGTC
CGGCAAACTTTCTCTGAACTCCGGAGAAAAGAAATCAATCCGTCAGCAGATGGAAGTGAGGAATCCGAAACTCTGGTCAC
CGGATGCTCCTTATCTATATAGAGTACAATCCCGCATAAAGAAAGGAAACCGTTCCATTGACGGAGGAACGACCCGTATC
GGTATCCGCCTAGCGGAATTTCGTGGCAAGGAGGGTTTCTGGCTGAATGGAAAACCTTTCGGACAATTAGTCGGAGCCAA
TCGTCATCAGGACTTCGCATACGTAGGCAATGCTCTTCCCAATTCACAGCAATGGCGTGATGCAAAGCGTCTGCGTGATG
CAGGATGTACCATCATCCGTGTAGCGCATTACCCGCAGGACCCATCCTTTATGGATGCCTGTGACGAACTCGGCTTGTTT
GTCATCGTCGCTACTCCTGGCTGGCAATACTGGAACAAAGACCCGAAATTCGGAGAACTGGTTCATCAGAATACCCGTGA
GATGATTCGTCGTGACCGCAATCATCCTTCTGTGCTGATGTGGGAGCCCATTCTGAATGAGACTCGCTATCCGCTCGACT
TTGCACTGAAGGCACTTGAAATTACGAAGGAAGAATATCCGTATCCGGGACGTCCGATAGCCGCTGCCGATGTGCATTCT
GCCGGTGTAAAGGAACATTACGATGTAGTCTACGGCTGGCCGGGTGATGACGAAAAGGAGGACAAACCGGAACAATGCAT
CTTCACCCGTGAATTCGGAGAAAACGTAGATGACTGGTATGCCCACAACAACAATAACCGTGCAAGCCGCAGTTGGGGTG
AACGTCCGCTGCTGATACAGGCACTTTCTCTGGCTAAAAGTTATGATGAGATGTACCGCACTACCGGTCAGTTTATCGGT
GGCACACAGTGGCATCCATTCGATCACCAGCGTGGTTATCACCCCGATCCATACTGGGGAGGCATTTATGACGCTTTCCG
TCAGAAAAAGTATGCGTATGAAATGTTCCGCAGCCAATCTCCGGCAAGCCTCCAACATCCGTTGGCAGAATGCGGACCGA
TGATATTCCTTGCTCATGAAATGTCACAATTCTCCGATAAGGATGTAGTAGTATTCAGTAACTGTGACTCCATCCGCCTT
TCTATCTACGATGGAACAAAAACATGGACCAAACCTGTGGTACATGCCAAAGGGCATATGCCGAACGCACCTGTCATCTT
CGAAAATGTATGGGATTTCTGGGAAGCGCGCGGATACAGTTATACTCAAAAGAACTGGCAGAAAGTAAATATGGTTGCCG
AAGGTATTATCGACGGGAAAGTAGTATGTACCCAAAAGAAAATGCCTTCCCGCCGGTCTACCAAACTTCGCTTGTATGCA
GATACTCAAAAAGTAAACTTGGTAGCGGACGGTTCGGATTTTATAGTAGTAGTTGCCGAAGTGACGGACGATAGCGGAAA
CGTTCGTCGTCTGGCAAAGGAAAATATCGTATTCACGGTAGAAGGGGAAGGAGAAATCATTGGTGACGCAAGGATCGGTG
CCAACCCTCGTGCCGTAGAATTCGGTTCGGCTCCTCTGCTGATCCGTTCCACACGAAAAGCCGGGAAGATTAAAGTGAAA
GCTCATGTACAATTTGAAGGGACTCAGGCTCCGACTGCCGCTGAAATGGAATTTGAAAGTGTTCCTGCGGAGCTTCCTTT
CTGTTATGATGAGAAGTACTGTATTAGTCAGGACACACCCTCATTAACTGTAAAAGCTACTTTATCCGAGAATGCAACCG
ACGGCAAAGTGCAACTGACAGAAGAAGAACGACAACGGGTGTTGGATGAAGTGGAACGGCAACAAACTGAATTTGGAATA
GAAAAGTGA

Upstream 100 bases:

>100_bases
GGAAAAGTAACAATCCTAAAGGTGTCACCGGAAAGCAGAAAGAAAGACATTGTTATAATGATAGAAAAATAAAAAAAAGA
ATACATAAATAAAAAGCACT

Downstream 100 bases:

>100_bases
GCTTGGTCGAAACTGAATGACAGATCAATATGCTTCATTCTTTCAGACCTGAGTTATATCGAACATAGGCTATTAGCAAA
AATGAATAAGCTAATAGCTA

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 882; Mature: 882

Protein sequence:

>882_residues
MNKRPFLILSLFLLLFSFDGQAADTYRPEISVAGFIPLPDCGRQVYNFNPGWRFFKGDIRGAEAVNFDDRSWEVVSTPHT
VELMPAEGSGCRNYQGPAWYRKHFVVPTATKGQRVLLHFEAAMGKQVLYLNGKRVQEHLGGYLPFTLDLTANGVQAGDSC
LLAVYTDNSNDKSYPPGKPQYTLDFAYHGGIYRDVWMIAKSPVAITDAIDSRTVGGGGVFVHFDKISEKSAQVYVETEIQ
NDNTRSESVTIETTLTDAEGNVIKRTSGKLSLNSGEKKSIRQQMEVRNPKLWSPDAPYLYRVQSRIKKGNRSIDGGTTRI
GIRLAEFRGKEGFWLNGKPFGQLVGANRHQDFAYVGNALPNSQQWRDAKRLRDAGCTIIRVAHYPQDPSFMDACDELGLF
VIVATPGWQYWNKDPKFGELVHQNTREMIRRDRNHPSVLMWEPILNETRYPLDFALKALEITKEEYPYPGRPIAAADVHS
AGVKEHYDVVYGWPGDDEKEDKPEQCIFTREFGENVDDWYAHNNNNRASRSWGERPLLIQALSLAKSYDEMYRTTGQFIG
GTQWHPFDHQRGYHPDPYWGGIYDAFRQKKYAYEMFRSQSPASLQHPLAECGPMIFLAHEMSQFSDKDVVVFSNCDSIRL
SIYDGTKTWTKPVVHAKGHMPNAPVIFENVWDFWEARGYSYTQKNWQKVNMVAEGIIDGKVVCTQKKMPSRRSTKLRLYA
DTQKVNLVADGSDFIVVVAEVTDDSGNVRRLAKENIVFTVEGEGEIIGDARIGANPRAVEFGSAPLLIRSTRKAGKIKVK
AHVQFEGTQAPTAAEMEFESVPAELPFCYDEKYCISQDTPSLTVKATLSENATDGKVQLTEEERQRVLDEVERQQTEFGI
EK

Sequences:

>Translated_882_residues
MNKRPFLILSLFLLLFSFDGQAADTYRPEISVAGFIPLPDCGRQVYNFNPGWRFFKGDIRGAEAVNFDDRSWEVVSTPHT
VELMPAEGSGCRNYQGPAWYRKHFVVPTATKGQRVLLHFEAAMGKQVLYLNGKRVQEHLGGYLPFTLDLTANGVQAGDSC
LLAVYTDNSNDKSYPPGKPQYTLDFAYHGGIYRDVWMIAKSPVAITDAIDSRTVGGGGVFVHFDKISEKSAQVYVETEIQ
NDNTRSESVTIETTLTDAEGNVIKRTSGKLSLNSGEKKSIRQQMEVRNPKLWSPDAPYLYRVQSRIKKGNRSIDGGTTRI
GIRLAEFRGKEGFWLNGKPFGQLVGANRHQDFAYVGNALPNSQQWRDAKRLRDAGCTIIRVAHYPQDPSFMDACDELGLF
VIVATPGWQYWNKDPKFGELVHQNTREMIRRDRNHPSVLMWEPILNETRYPLDFALKALEITKEEYPYPGRPIAAADVHS
AGVKEHYDVVYGWPGDDEKEDKPEQCIFTREFGENVDDWYAHNNNNRASRSWGERPLLIQALSLAKSYDEMYRTTGQFIG
GTQWHPFDHQRGYHPDPYWGGIYDAFRQKKYAYEMFRSQSPASLQHPLAECGPMIFLAHEMSQFSDKDVVVFSNCDSIRL
SIYDGTKTWTKPVVHAKGHMPNAPVIFENVWDFWEARGYSYTQKNWQKVNMVAEGIIDGKVVCTQKKMPSRRSTKLRLYA
DTQKVNLVADGSDFIVVVAEVTDDSGNVRRLAKENIVFTVEGEGEIIGDARIGANPRAVEFGSAPLLIRSTRKAGKIKVK
AHVQFEGTQAPTAAEMEFESVPAELPFCYDEKYCISQDTPSLTVKATLSENATDGKVQLTEEERQRVLDEVERQQTEFGI
EK
>Mature_882_residues
MNKRPFLILSLFLLLFSFDGQAADTYRPEISVAGFIPLPDCGRQVYNFNPGWRFFKGDIRGAEAVNFDDRSWEVVSTPHT
VELMPAEGSGCRNYQGPAWYRKHFVVPTATKGQRVLLHFEAAMGKQVLYLNGKRVQEHLGGYLPFTLDLTANGVQAGDSC
LLAVYTDNSNDKSYPPGKPQYTLDFAYHGGIYRDVWMIAKSPVAITDAIDSRTVGGGGVFVHFDKISEKSAQVYVETEIQ
NDNTRSESVTIETTLTDAEGNVIKRTSGKLSLNSGEKKSIRQQMEVRNPKLWSPDAPYLYRVQSRIKKGNRSIDGGTTRI
GIRLAEFRGKEGFWLNGKPFGQLVGANRHQDFAYVGNALPNSQQWRDAKRLRDAGCTIIRVAHYPQDPSFMDACDELGLF
VIVATPGWQYWNKDPKFGELVHQNTREMIRRDRNHPSVLMWEPILNETRYPLDFALKALEITKEEYPYPGRPIAAADVHS
AGVKEHYDVVYGWPGDDEKEDKPEQCIFTREFGENVDDWYAHNNNNRASRSWGERPLLIQALSLAKSYDEMYRTTGQFIG
GTQWHPFDHQRGYHPDPYWGGIYDAFRQKKYAYEMFRSQSPASLQHPLAECGPMIFLAHEMSQFSDKDVVVFSNCDSIRL
SIYDGTKTWTKPVVHAKGHMPNAPVIFENVWDFWEARGYSYTQKNWQKVNMVAEGIIDGKVVCTQKKMPSRRSTKLRLYA
DTQKVNLVADGSDFIVVVAEVTDDSGNVRRLAKENIVFTVEGEGEIIGDARIGANPRAVEFGSAPLLIRSTRKAGKIKVK
AHVQFEGTQAPTAAEMEFESVPAELPFCYDEKYCISQDTPSLTVKATLSENATDGKVQLTEEERQRVLDEVERQQTEFGI
EK

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=422, Percent_Identity=26.7772511848341, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787903, Length=496, Percent_Identity=29.0322580645161, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI48994920, Length=381, Percent_Identity=26.509186351706, Blast_Score=116, Evalue=8e-27,
Organism=Escherichia coli, GI1786539, Length=326, Percent_Identity=25.4601226993865, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17510825, Length=383, Percent_Identity=25.065274151436, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24655438, Length=480, Percent_Identity=25, Blast_Score=120, Evalue=6e-27,
Organism=Drosophila melanogaster, GI19922582, Length=480, Percent_Identity=25, Blast_Score=120, Evalue=6e-27,
Organism=Drosophila melanogaster, GI62471735, Length=480, Percent_Identity=25, Blast_Score=120, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24651745, Length=514, Percent_Identity=24.3190661478599, Blast_Score=118, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 99828; Mature: 99828

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRPFLILSLFLLLFSFDGQAADTYRPEISVAGFIPLPDCGRQVYNFNPGWRFFKGDIR
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHEECCCCCEEEECCCC
GAEAVNFDDRSWEVVSTPHTVELMPAEGSGCRNYQGPAWYRKHFVVPTATKGQRVLLHFE
CCEEECCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHEEEEEECCCCCCEEEEEEE
AAMGKQVLYLNGKRVQEHLGGYLPFTLDLTANGVQAGDSCLLAVYTDNSNDKSYPPGKPQ
CCCCCEEEEECCHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCC
YTLDFAYHGGIYRDVWMIAKSPVAITDAIDSRTVGGGGVFVHFDKISEKSAQVYVETEIQ
EEEEEEECCCCHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEEEEEEC
NDNTRSESVTIETTLTDAEGNVIKRTSGKLSLNSGEKKSIRQQMEVRNPKLWSPDAPYLY
CCCCCCCEEEEEEEEECCCCCEEEECCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHH
RVQSRIKKGNRSIDGGTTRIGIRLAEFRGKEGFWLNGKPFGQLVGANRHQDFAYVGNALP
HHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCEEECCCCHHHHCCCCCCCCHHHHCCCCC
NSQQWRDAKRLRDAGCTIIRVAHYPQDPSFMDACDELGLFVIVATPGWQYWNKDPKFGEL
CCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCHHHH
VHQNTREMIRRDRNHPSVLMWEPILNETRYPLDFALKALEITKEEYPYPGRPIAAADVHS
HHHHHHHHHHHCCCCCCEEEECHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCHH
AGVKEHYDVVYGWPGDDEKEDKPEQCIFTREFGENVDDWYAHNNNNRASRSWGERPLLIQ
CCCCCCCEEEEECCCCCCCCCCHHHEEEEHHHCCCCHHHHEECCCCCCCCCCCCCCHHHH
ALSLAKSYDEMYRTTGQFIGGTQWHPFDHQRGYHPDPYWGGIYDAFRQKKYAYEMFRSQS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PASLQHPLAECGPMIFLAHEMSQFSDKDVVVFSNCDSIRLSIYDGTKTWTKPVVHAKGHM
CCHHHCCHHHHCCEEEEEEHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCCEEEECCCC
PNAPVIFENVWDFWEARGYSYTQKNWQKVNMVAEGIIDGKVVCTQKKMPSRRSTKLRLYA
CCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEE
DTQKVNLVADGSDFIVVVAEVTDDSGNVRRLAKENIVFTVEGEGEIIGDARIGANPRAVE
CCCEEEEEECCCCEEEEEEEEECCCCCHHHHHHCCEEEEEECCCEEEECCEECCCCCEEE
FGSAPLLIRSTRKAGKIKVKAHVQFEGTQAPTAAEMEFESVPAELPFCYDEKYCISQDTP
ECCCCEEEEECCCCCEEEEEEEEEECCCCCCCHHCCCHHCCCCCCCEEECCCEEECCCCC
SLTVKATLSENATDGKVQLTEEERQRVLDEVERQQTEFGIEK
CEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKRPFLILSLFLLLFSFDGQAADTYRPEISVAGFIPLPDCGRQVYNFNPGWRFFKGDIR
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHEECCCCCEEEECCCC
GAEAVNFDDRSWEVVSTPHTVELMPAEGSGCRNYQGPAWYRKHFVVPTATKGQRVLLHFE
CCEEECCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHEEEEEECCCCCCEEEEEEE
AAMGKQVLYLNGKRVQEHLGGYLPFTLDLTANGVQAGDSCLLAVYTDNSNDKSYPPGKPQ
CCCCCEEEEECCHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCC
YTLDFAYHGGIYRDVWMIAKSPVAITDAIDSRTVGGGGVFVHFDKISEKSAQVYVETEIQ
EEEEEEECCCCHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEEEEEEC
NDNTRSESVTIETTLTDAEGNVIKRTSGKLSLNSGEKKSIRQQMEVRNPKLWSPDAPYLY
CCCCCCCEEEEEEEEECCCCCEEEECCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHH
RVQSRIKKGNRSIDGGTTRIGIRLAEFRGKEGFWLNGKPFGQLVGANRHQDFAYVGNALP
HHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCEEECCCCHHHHCCCCCCCCHHHHCCCCC
NSQQWRDAKRLRDAGCTIIRVAHYPQDPSFMDACDELGLFVIVATPGWQYWNKDPKFGEL
CCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCHHHH
VHQNTREMIRRDRNHPSVLMWEPILNETRYPLDFALKALEITKEEYPYPGRPIAAADVHS
HHHHHHHHHHHCCCCCCEEEECHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCHH
AGVKEHYDVVYGWPGDDEKEDKPEQCIFTREFGENVDDWYAHNNNNRASRSWGERPLLIQ
CCCCCCCEEEEECCCCCCCCCCHHHEEEEHHHCCCCHHHHEECCCCCCCCCCCCCCHHHH
ALSLAKSYDEMYRTTGQFIGGTQWHPFDHQRGYHPDPYWGGIYDAFRQKKYAYEMFRSQS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PASLQHPLAECGPMIFLAHEMSQFSDKDVVVFSNCDSIRLSIYDGTKTWTKPVVHAKGHM
CCHHHCCHHHHCCEEEEEEHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCCEEEECCCC
PNAPVIFENVWDFWEARGYSYTQKNWQKVNMVAEGIIDGKVVCTQKKMPSRRSTKLRLYA
CCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEE
DTQKVNLVADGSDFIVVVAEVTDDSGNVRRLAKENIVFTVEGEGEIIGDARIGANPRAVE
CCCEEEEEECCCCEEEEEEEEECCCCCHHHHHHCCEEEEEECCCEEEECCEECCCCCEEE
FGSAPLLIRSTRKAGKIKVKAHVQFEGTQAPTAAEMEFESVPAELPFCYDEKYCISQDTP
ECCCCEEEEECCCCCEEEEEEEEEECCCCCCCHHCCCHHCCCCCCCEEECCCEEECCCCC
SLTVKATLSENATDGKVQLTEEERQRVLDEVERQQTEFGIEK
CEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA