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The map label for this gene is rimO

Identifier: 29346323

GI number: 29346323

Start: 1124016

End: 1125326

Strand: Reverse

Name: rimO

Synonym: BT_0913

Alternate gene names: 29346323

Gene position: 1125326-1124016 (Counterclockwise)

Preceding gene: 29346324

Following gene: 29346322

Centisome position: 17.98

GC content: 43.1

Gene sequence:

>1311_bases
ATGAAAAGAAAGAGAATAGATATTATTACTTTAGGTTGTTCTAAAAATTTAGTAGACTCGGAGCAGTTGATGCGTCAGCT
GGAAGAAGCAGGATACAGCGTAACTCATGATACAGAAAATCCGGAAGGGGAGATAGCGGTAATCAACACCTGCGGTTTCA
TCGGTGACGCGAAAGAAGAGTCTATCAATATGATTCTGGAATTTGCAGAAAGAAAAGAAGAAGGCGACTTGAAGAAACTG
TTTGTAATGGGCTGTCTTTCCGAGCGTTATTTACAAGAACTGGCCATTGAAATACCACAGGTAGACAAGTTCTACGGCAA
ATTCAACTGGAAAGAACTCTTGCAGGACTTGGGAAAAACGTACCATGAGGAATTGTATATTGAGCGTACTTTGACCACTC
CCAAGCATTATGCTTATCTGAAGATTTCAGAAGGATGTGACCGCAAGTGCTCTTATTGTGCTATTCCCATTATTACAGGA
CGCCATATATCCAAGTCGATGGAAGAAATACTGGATGAAGTCCGGTATCTGGTATCGCAAGGGGTAAAGGAGTTTCAGGT
GATCGCTCAGGAATTGACGTATTACGGAGTCGATCTGTATAAAAAGCAAATGCTGCCCGAATTGATCGAACGTATCTCGG
AAATTCCGGGTGTAGAGTGGATTCGTCTGCATTATGCGTATCCGGCACATTTTCCAACGGATTTATTTCGGGTGATGCGG
GAACGCGATAATGTCTGCAAATATATGGATATAGCCTTGCAGCATATCAGTGACAACATGTTGAAACTGATGCGTCGGCA
AGTAAGCAAAGAAGATACGTACAAGCTGATCGAACAGTTCCGTAAAGAAGTACCGGGTATCCATCTGCGCACTACGCTGA
TGGTAGGACATCCCGGAGAAACGGAAGAAGACTTTGAAGAGCTGAAAGAATTTGTACGCAAGGCGCGTTTCGACAGGATG
GGGGCTTTCGCTTATTCTGAAGAAGAGGGTACTTATGCTGCCCAGCAATATGAAGACTCTATACCACAGGAGGTTAAACA
AGCCCGACTGGACGAGCTAATGGATATCCAGCAAGGTATTTCAGCAGAATTGAGCGCTGCAAAAATTGGTCAGCAGATGA
AAGTCATCATCGACCGGATCGAGGGAGATTATTATATCGGACGTACGGAATTTGATTCACCGGAGGTAGACCCAGAAGTA
TTGATCAGTGTTTCCAGAGAAGAACTTGAAGTCGGACAATTCTATCAGGTAGAAGTAACCGATGCGGACGATTTTGATTT
ATATGCAAAGATATTAAACAAGTATGAATAA

Upstream 100 bases:

>100_bases
TCAAGATTCCTGTAAAATACATTGGATTGGGTGAAGGCATGGAAGATTTGCAAGTCTTCCGCAAAAATGAATTTGTTGAT
TCCCTGTTTGGAGAGAATGC

Downstream 100 bases:

>100_bases
TAAGGAATTTACATCGGAACTGGCAGACAGACTGGGGTATACTATCAAAGACACTTCTGAACTGATAAGTTCTTTATTGT
CTGACATGACACAGGAACTG

Product: putative Fe-S oxidoreductase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKRKRIDIITLGCSKNLVDSEQLMRQLEEAGYSVTHDTENPEGEIAVINTCGFIGDAKEESINMILEFAERKEEGDLKKL
FVMGCLSERYLQELAIEIPQVDKFYGKFNWKELLQDLGKTYHEELYIERTLTTPKHYAYLKISEGCDRKCSYCAIPIITG
RHISKSMEEILDEVRYLVSQGVKEFQVIAQELTYYGVDLYKKQMLPELIERISEIPGVEWIRLHYAYPAHFPTDLFRVMR
ERDNVCKYMDIALQHISDNMLKLMRRQVSKEDTYKLIEQFRKEVPGIHLRTTLMVGHPGETEEDFEELKEFVRKARFDRM
GAFAYSEEEGTYAAQQYEDSIPQEVKQARLDELMDIQQGISAELSAAKIGQQMKVIIDRIEGDYYIGRTEFDSPEVDPEV
LISVSREELEVGQFYQVEVTDADDFDLYAKILNKYE

Sequences:

>Translated_436_residues
MKRKRIDIITLGCSKNLVDSEQLMRQLEEAGYSVTHDTENPEGEIAVINTCGFIGDAKEESINMILEFAERKEEGDLKKL
FVMGCLSERYLQELAIEIPQVDKFYGKFNWKELLQDLGKTYHEELYIERTLTTPKHYAYLKISEGCDRKCSYCAIPIITG
RHISKSMEEILDEVRYLVSQGVKEFQVIAQELTYYGVDLYKKQMLPELIERISEIPGVEWIRLHYAYPAHFPTDLFRVMR
ERDNVCKYMDIALQHISDNMLKLMRRQVSKEDTYKLIEQFRKEVPGIHLRTTLMVGHPGETEEDFEELKEFVRKARFDRM
GAFAYSEEEGTYAAQQYEDSIPQEVKQARLDELMDIQQGISAELSAAKIGQQMKVIIDRIEGDYYIGRTEFDSPEVDPEV
LISVSREELEVGQFYQVEVTDADDFDLYAKILNKYE
>Mature_436_residues
MKRKRIDIITLGCSKNLVDSEQLMRQLEEAGYSVTHDTENPEGEIAVINTCGFIGDAKEESINMILEFAERKEEGDLKKL
FVMGCLSERYLQELAIEIPQVDKFYGKFNWKELLQDLGKTYHEELYIERTLTTPKHYAYLKISEGCDRKCSYCAIPIITG
RHISKSMEEILDEVRYLVSQGVKEFQVIAQELTYYGVDLYKKQMLPELIERISEIPGVEWIRLHYAYPAHFPTDLFRVMR
ERDNVCKYMDIALQHISDNMLKLMRRQVSKEDTYKLIEQFRKEVPGIHLRTTLMVGHPGETEEDFEELKEFVRKARFDRM
GAFAYSEEEGTYAAQQYEDSIPQEVKQARLDELMDIQQGISAELSAAKIGQQMKVIIDRIEGDYYIGRTEFDSPEVDPEV
LISVSREELEVGQFYQVEVTDADDFDLYAKILNKYE

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=484, Percent_Identity=24.5867768595041, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI28872784, Length=484, Percent_Identity=24.3801652892562, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI93277076, Length=414, Percent_Identity=24.6376811594203, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1787057, Length=445, Percent_Identity=37.9775280898876, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=25.1670378619154, Blast_Score=151, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=471, Percent_Identity=23.3545647558386, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71996771, Length=296, Percent_Identity=26.6891891891892, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356207, Length=465, Percent_Identity=24.5161290322581, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI19922432, Length=452, Percent_Identity=25.8849557522124, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_BACTN (Q8A9A2)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_809826.1
- ProteinModelPortal:   Q8A9A2
- GeneID:   1073427
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_0913
- NMPDR:   fig|226186.1.peg.913
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q8A9A2
- ProtClustDB:   CLSK822448
- BioCyc:   BTHE226186:BT_0913-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50870; Mature: 50870

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKRIDIITLGCSKNLVDSEQLMRQLEEAGYSVTHDTENPEGEIAVINTCGFIGDAKEE
CCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCCCHHH
SINMILEFAERKEEGDLKKLFVMGCLSERYLQELAIEIPQVDKFYGKFNWKELLQDLGKT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHH
YHEELYIERTLTTPKHYAYLKISEGCDRKCSYCAIPIITGRHISKSMEEILDEVRYLVSQ
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHH
GVKEFQVIAQELTYYGVDLYKKQMLPELIERISEIPGVEWIRLHYAYPAHFPTDLFRVMR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
ERDNVCKYMDIALQHISDNMLKLMRRQVSKEDTYKLIEQFRKEVPGIHLRTTLMVGHPGE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
TEEDFEELKEFVRKARFDRMGAFAYSEEEGTYAAQQYEDSIPQEVKQARLDELMDIQQGI
CHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
SAELSAAKIGQQMKVIIDRIEGDYYIGRTEFDSPEVDPEVLISVSREELEVGQFYQVEVT
CHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCHHHHCCCCEEEEEEC
DADDFDLYAKILNKYE
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKRKRIDIITLGCSKNLVDSEQLMRQLEEAGYSVTHDTENPEGEIAVINTCGFIGDAKEE
CCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCCCHHH
SINMILEFAERKEEGDLKKLFVMGCLSERYLQELAIEIPQVDKFYGKFNWKELLQDLGKT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHH
YHEELYIERTLTTPKHYAYLKISEGCDRKCSYCAIPIITGRHISKSMEEILDEVRYLVSQ
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHH
GVKEFQVIAQELTYYGVDLYKKQMLPELIERISEIPGVEWIRLHYAYPAHFPTDLFRVMR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
ERDNVCKYMDIALQHISDNMLKLMRRQVSKEDTYKLIEQFRKEVPGIHLRTTLMVGHPGE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
TEEDFEELKEFVRKARFDRMGAFAYSEEEGTYAAQQYEDSIPQEVKQARLDELMDIQQGI
CHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
SAELSAAKIGQQMKVIIDRIEGDYYIGRTEFDSPEVDPEVLISVSREELEVGQFYQVEVT
CHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCHHHHCCCCEEEEEEC
DADDFDLYAKILNKYE
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928