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The map label for this gene is alaS [H]

Identifier: 29345976

GI number: 29345976

Start: 702524

End: 703000

Strand: Reverse

Name: alaS [H]

Synonym: BT_0566

Alternate gene names: 29345976

Gene position: 703000-702524 (Counterclockwise)

Preceding gene: 29345977

Following gene: 29345975

Centisome position: 11.23

GC content: 39.41

Gene sequence:

>477_bases
ATGGAACAACAACCTCAACTCAATGATCACAACAAACAGGAGTATCCTCCTATGCATACAGCAGAGCATTTACTAAATGC
CACTATGGTAAAAACTTTCGGTTGCCCTCGCTCAAGAAATGCCCATATAGAAAGGAAAAAGAGTAAGTGCGACTATATTC
TTCCTACTTGTCCTACAGATGCGGAGATACAAGCGATAGAAGATAAGGTAAACGAAGTTATCAGTCAAAATCTGCCCGTA
ACTGTTGAATTTATGACTCATGAGCAAGCTAAAGGCATCGTAGATCTGAGCAAACTTCCTGCTGACGCCAGTGAGACTCT
TCGCATTATACGCATAGGAGATTATGACGCCTGTGCCTGTATAGGGTTACATGTATCCAATACCTCAGAGGTAGGAACTT
TCAAAATCATCAGTCATGATTATAATGAAGAAAGGCAAACTTTAAGGTTAAGATTCAAACTCATTGAAAAAAAATAG

Upstream 100 bases:

>100_bases
ATTTAGCGATAGATAAGCAACCGCATTTCCTGGGAGAAAAGTACAAACTGCCATCCGGCAAATAAGAAAATATCAAAAAC
AAAAAGTTATTGAAAAATTT

Downstream 100 bases:

>100_bases
ATTATCAAATTAATATCTATATTATATAAAATAAATATACTGCGGTTACATTTTGTCACAAAACAGGCAAAATTGGCATT
ATTAAAATGACAAATAGACA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MEQQPQLNDHNKQEYPPMHTAEHLLNATMVKTFGCPRSRNAHIERKKSKCDYILPTCPTDAEIQAIEDKVNEVISQNLPV
TVEFMTHEQAKGIVDLSKLPADASETLRIIRIGDYDACACIGLHVSNTSEVGTFKIISHDYNEERQTLRLRFKLIEKK

Sequences:

>Translated_158_residues
MEQQPQLNDHNKQEYPPMHTAEHLLNATMVKTFGCPRSRNAHIERKKSKCDYILPTCPTDAEIQAIEDKVNEVISQNLPV
TVEFMTHEQAKGIVDLSKLPADASETLRIIRIGDYDACACIGLHVSNTSEVGTFKIISHDYNEERQTLRLRFKLIEKK
>Mature_158_residues
MEQQPQLNDHNKQEYPPMHTAEHLLNATMVKTFGCPRSRNAHIERKKSKCDYILPTCPTDAEIQAIEDKVNEVISQNLPV
TVEFMTHEQAKGIVDLSKLPADASETLRIIRIGDYDACACIGLHVSNTSEVGTFKIISHDYNEERQTLRLRFKLIEKK

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789048, Length=121, Percent_Identity=31.404958677686, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 18033; Mature: 18033

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQQPQLNDHNKQEYPPMHTAEHLLNATMVKTFGCPRSRNAHIERKKSKCDYILPTCPTD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCEECCCCCCC
AEIQAIEDKVNEVISQNLPVTVEFMTHEQAKGIVDLSKLPADASETLRIIRIGDYDACAC
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHCCCCCCCCEEEEEECCCCCEEE
IGLHVSNTSEVGTFKIISHDYNEERQTLRLRFKLIEKK
EEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHEECCC
>Mature Secondary Structure
MEQQPQLNDHNKQEYPPMHTAEHLLNATMVKTFGCPRSRNAHIERKKSKCDYILPTCPTD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCEECCCCCCC
AEIQAIEDKVNEVISQNLPVTVEFMTHEQAKGIVDLSKLPADASETLRIIRIGDYDACAC
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHCCCCCCCCEEEEEECCCCCEEE
IGLHVSNTSEVGTFKIISHDYNEERQTLRLRFKLIEKK
EEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA