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The map label for this gene is nagB [C]

Identifier: 29345668

GI number: 29345668

Start: 288339

End: 290330

Strand: Reverse

Name: nagB [C]

Synonym: BT_0258

Alternate gene names: 29345668

Gene position: 290330-288339 (Counterclockwise)

Preceding gene: 29345669

Following gene: 29345667

Centisome position: 4.64

GC content: 45.43

Gene sequence:

>1992_bases
ATGAAAACAAATCTTAGTTCTCAAATCACTCTCCACAGGGTCTCCCCCAGATACTACAGACCGGAGAATGCATTTGAAAA
ATCAGTCTTGACAAGACTCGAGAAAATACCTACAGACATTTATGAGTCTGTAGAAGAAGGGGCTAACTACATTGCTTGCG
AAATAGCACAAACCATCCGCGAAAAACAGAAAGCAGGACGCTTTTGCGTACTAGCACTCCCCGGAGGTAACTCACCCAGC
CATGTCTATTCGGAACTGATCCGCATGCATAAGGAAGAAGGACTGAGCTTCCGTAACGTGATCGTATTTAATATGTATGA
ATACTATCCGTTGACAGCCGATGCTATCAACAGCAACTTCAATGCTTTAAAAGAAATGTTGCTGGATCATGTCGATATTG
ACAAACAGAATATATTCACTCCCGATGGAACCATCGCCAAAGATACCATCTTTGAATATTGCCGTCTGTACGAACAGCGC
ATCGAAAGTTTCGGAGGTATTGACATTGCCCTGCTGGGTATCGGTCGTGTAGGTAATATCGCATTCAACGAACCGGGTTC
ACGTCTGAACTCTACCACACGTCTGATCTTATTGGACAATGCATCGCGCAACGAAGCATCCAAGATTTTCGGAACAATAG
AAAATACTCCGATCAGTTCTATCACAATGGGAGTATCTACCATTCTTGGCGCTAAAAAGGTATATTTGCTGGCATGGGGG
GAAAATAAGGCAGCAATGATCAAAGAATGTGTGGAAGGTCCGATTAGTGACACAATTCCTGCATCTTATCTGCAAACTCA
CAACAATGCTCATGTAGCGATCGACCTTTCGGCTTCCATGAACTTAACACGCATTCAACGTCCGTGGCTGGTAACATCCT
GCGAGTGGAATGATAAACTGATCCGCAGCGCTATCGTATGGCTGTGCCAGTTGACAGGCAAACCGATTCTGAAGCTGACC
AACAAGGACTACAACGAAAACGGTCTGAGCGAACTTCTGGCATTGTTTGGTTCTGCTTACAATGTAAATATCAAGATTTT
CAACGACTTGCAGCATACCATCACCGGATGGCCGGGTGGCAAACCTAATGCTGACGATACTTATCGTCCGGAACGTGCCA
AACCGTATCCGAAACGTGTGGTTATCTTCTCTCCGCATCCGGATGACGACGTTATCTCCATGGGTGGAACCCTCCGCCGC
CTGGTAGAACAGAAGCATGAGGTACACGTAGCTTACGAAACTTCCGGTAACATTGCCGTAGGTGATGAGGAAGTGGTTCG
TTTCATGCATTTCATTAACGGATTCAATCAGATTTTCAATAACAGCGAAGACTTGGTAATCAGCGAGAAATATGCAGAAA
TACGCAAATTCCTGAAAGAAAAGAAAGACGGTGATATGGACAGCCGTGACATTCTGACTATCAAAGGTTTGATTCGTCGT
GGTGAAGCCCGTACCGCTTCTTCATACAATAATATTCCGCTAGATCGGGTACATTTCCTTGACTTGCCGTTCTATGAAAC
CGGTAAGATTCAGAAAAATCCGATTAGCGAGGCTGACGTAGAGATCGTACGTAACCTGCTTCGTGAAATAAAGCCTCATC
AGATTTTCGTAGCCGGTGATTTGGCTGACCCACACGGTACACACCGTGTATGTACGGATGCCGTATTCGCTGCTGTCGAT
CTTGAAAAAGAAGAAGGAGCCAAATGGTTGAAAGACTGTCGTATCTGGATGTATCGTGGTGCGTGGGCTGAATGGGAAAT
TGAGAACATCGAAATGGCTGTGCCTATCAGTCCGGAAGAGCTTCGTGCAAAACGTAATTCTATCCTGAAGCATCAGTCGC
AGATGGAAAGCGCTCCGTTCTTAGGTAATGACGAACGTCTGTTCTGGCAACGTAGCGAAGACCGTAACCGTGGCACAGCA
ACGTTGTATGATCAGTTAGGACTGGCATCGTATGAGGCAATGGAGGCATTTGTAGAATACATTCCTCTATAA

Upstream 100 bases:

>100_bases
CCTAGAAGTTAGTAGTTAATTATATAGACTTCTTTTGTATCTTTGTCGTGAAAATACAGTCGTAAGGACTCTAATCTTAC
ATAACTTAATTAACAACTTT

Downstream 100 bases:

>100_bases
GAAAGAAAATTCTTAATTAATAGAATATAAAACAATCAAAGAAGAGTGGGCAGTTCGATACTTCCCACTCTTTTTTTGTT
TATTTTTGCAGGAGATTTTG

Product: glucosamine-6-phosphate deaminase-like protein

Products: D-fructose 6-phosphate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 663; Mature: 663

Protein sequence:

>663_residues
MKTNLSSQITLHRVSPRYYRPENAFEKSVLTRLEKIPTDIYESVEEGANYIACEIAQTIREKQKAGRFCVLALPGGNSPS
HVYSELIRMHKEEGLSFRNVIVFNMYEYYPLTADAINSNFNALKEMLLDHVDIDKQNIFTPDGTIAKDTIFEYCRLYEQR
IESFGGIDIALLGIGRVGNIAFNEPGSRLNSTTRLILLDNASRNEASKIFGTIENTPISSITMGVSTILGAKKVYLLAWG
ENKAAMIKECVEGPISDTIPASYLQTHNNAHVAIDLSASMNLTRIQRPWLVTSCEWNDKLIRSAIVWLCQLTGKPILKLT
NKDYNENGLSELLALFGSAYNVNIKIFNDLQHTITGWPGGKPNADDTYRPERAKPYPKRVVIFSPHPDDDVISMGGTLRR
LVEQKHEVHVAYETSGNIAVGDEEVVRFMHFINGFNQIFNNSEDLVISEKYAEIRKFLKEKKDGDMDSRDILTIKGLIRR
GEARTASSYNNIPLDRVHFLDLPFYETGKIQKNPISEADVEIVRNLLREIKPHQIFVAGDLADPHGTHRVCTDAVFAAVD
LEKEEGAKWLKDCRIWMYRGAWAEWEIENIEMAVPISPEELRAKRNSILKHQSQMESAPFLGNDERLFWQRSEDRNRGTA
TLYDQLGLASYEAMEAFVEYIPL

Sequences:

>Translated_663_residues
MKTNLSSQITLHRVSPRYYRPENAFEKSVLTRLEKIPTDIYESVEEGANYIACEIAQTIREKQKAGRFCVLALPGGNSPS
HVYSELIRMHKEEGLSFRNVIVFNMYEYYPLTADAINSNFNALKEMLLDHVDIDKQNIFTPDGTIAKDTIFEYCRLYEQR
IESFGGIDIALLGIGRVGNIAFNEPGSRLNSTTRLILLDNASRNEASKIFGTIENTPISSITMGVSTILGAKKVYLLAWG
ENKAAMIKECVEGPISDTIPASYLQTHNNAHVAIDLSASMNLTRIQRPWLVTSCEWNDKLIRSAIVWLCQLTGKPILKLT
NKDYNENGLSELLALFGSAYNVNIKIFNDLQHTITGWPGGKPNADDTYRPERAKPYPKRVVIFSPHPDDDVISMGGTLRR
LVEQKHEVHVAYETSGNIAVGDEEVVRFMHFINGFNQIFNNSEDLVISEKYAEIRKFLKEKKDGDMDSRDILTIKGLIRR
GEARTASSYNNIPLDRVHFLDLPFYETGKIQKNPISEADVEIVRNLLREIKPHQIFVAGDLADPHGTHRVCTDAVFAAVD
LEKEEGAKWLKDCRIWMYRGAWAEWEIENIEMAVPISPEELRAKRNSILKHQSQMESAPFLGNDERLFWQRSEDRNRGTA
TLYDQLGLASYEAMEAFVEYIPL
>Mature_663_residues
MKTNLSSQITLHRVSPRYYRPENAFEKSVLTRLEKIPTDIYESVEEGANYIACEIAQTIREKQKAGRFCVLALPGGNSPS
HVYSELIRMHKEEGLSFRNVIVFNMYEYYPLTADAINSNFNALKEMLLDHVDIDKQNIFTPDGTIAKDTIFEYCRLYEQR
IESFGGIDIALLGIGRVGNIAFNEPGSRLNSTTRLILLDNASRNEASKIFGTIENTPISSITMGVSTILGAKKVYLLAWG
ENKAAMIKECVEGPISDTIPASYLQTHNNAHVAIDLSASMNLTRIQRPWLVTSCEWNDKLIRSAIVWLCQLTGKPILKLT
NKDYNENGLSELLALFGSAYNVNIKIFNDLQHTITGWPGGKPNADDTYRPERAKPYPKRVVIFSPHPDDDVISMGGTLRR
LVEQKHEVHVAYETSGNIAVGDEEVVRFMHFINGFNQIFNNSEDLVISEKYAEIRKFLKEKKDGDMDSRDILTIKGLIRR
GEARTASSYNNIPLDRVHFLDLPFYETGKIQKNPISEADVEIVRNLLREIKPHQIFVAGDLADPHGTHRVCTDAVFAAVD
LEKEEGAKWLKDCRIWMYRGAWAEWEIENIEMAVPISPEELRAKRNSILKHQSQMESAPFLGNDERLFWQRSEDRNRGTA
TLYDQLGLASYEAMEAFVEYIPL

Specific function: Catalyzes The Reversible Isomerization-Deamination Of Glucosamine 6-Phosphate (Glcn6p) To Form Fructose 6-Phosphate (Fru6p) And Ammonium Ion. [C]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI19923881, Length=245, Percent_Identity=34.6938775510204, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI13027378, Length=242, Percent_Identity=33.0578512396694, Blast_Score=115, Evalue=2e-25,
Organism=Escherichia coli, GI1786893, Length=222, Percent_Identity=37.8378378378378, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI1789530, Length=245, Percent_Identity=24.8979591836735, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI48994958, Length=203, Percent_Identity=25.615763546798, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17554876, Length=244, Percent_Identity=34.0163934426229, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24581960, Length=245, Percent_Identity=33.0612244897959, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19920764, Length=245, Percent_Identity=33.0612244897959, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y258_BACTN (Q8AB53)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_809171.1
- ProteinModelPortal:   Q8AB53
- SMR:   Q8AB53
- GeneID:   1074962
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_0258
- NMPDR:   fig|226186.1.peg.258
- HOGENOM:   HBG335312
- OMA:   HVAYQTS
- PhylomeDB:   Q8AB53
- ProtClustDB:   PRK02122
- BioCyc:   BTHE226186:BT_0258-MONOMER
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR003737
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280

Pfam domain/function: PF01182 Glucosamine_iso; PF02585 PIG-L

EC number: 3.5.99.6

Molecular weight: Translated: 75266; Mature: 75266

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 184-184

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNLSSQITLHRVSPRYYRPENAFEKSVLTRLEKIPTDIYESVEEGANYIACEIAQTIR
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEHHHHHHHHH
EKQKAGRFCVLALPGGNSPSHVYSELIRMHKEEGLSFRNVIVFNMYEYYPLTADAINSNF
HHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEHHHCCCCHHHHCCCH
NALKEMLLDHVDIDKQNIFTPDGTIAKDTIFEYCRLYEQRIESFGGIDIALLGIGRVGNI
HHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
AFNEPGSRLNSTTRLILLDNASRNEASKIFGTIENTPISSITMGVSTILGAKKVYLLAWG
EECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEC
ENKAAMIKECVEGPISDTIPASYLQTHNNAHVAIDLSASMNLTRIQRPWLVTSCEWNDKL
CCCHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEEECCCCCHHEECCCEEEEECCCCHHH
IRSAIVWLCQLTGKPILKLTNKDYNENGLSELLALFGSAYNVNIKIFNDLQHTITGWPGG
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEECCHHHHHCCCCCC
KPNADDTYRPERAKPYPKRVVIFSPHPDDDVISMGGTLRRLVEQKHEVHVAYETSGNIAV
CCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCHHHHHHHHHHCCEEEEEECCCCEEE
GDEEVVRFMHFINGFNQIFNNSEDLVISEKYAEIRKFLKEKKDGDMDSRDILTIKGLIRR
CHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
GEARTASSYNNIPLDRVHFLDLPFYETGKIQKNPISEADVEIVRNLLREIKPHQIFVAGD
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEC
LADPHGTHRVCTDAVFAAVDLEKEEGAKWLKDCRIWMYRGAWAEWEIENIEMAVPISPEE
CCCCCCCHHHHHHHHHHHEECCHHHHHHHHHHHHEEEEECCCCEEEECCEEEEECCCHHH
LRAKRNSILKHQSQMESAPFLGNDERLFWQRSEDRNRGTATLYDQLGLASYEAMEAFVEY
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEHHHHHCCHHHHHHHHHHHH
IPL
CCC
>Mature Secondary Structure
MKTNLSSQITLHRVSPRYYRPENAFEKSVLTRLEKIPTDIYESVEEGANYIACEIAQTIR
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEHHHHHHHHH
EKQKAGRFCVLALPGGNSPSHVYSELIRMHKEEGLSFRNVIVFNMYEYYPLTADAINSNF
HHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEHHHCCCCHHHHCCCH
NALKEMLLDHVDIDKQNIFTPDGTIAKDTIFEYCRLYEQRIESFGGIDIALLGIGRVGNI
HHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
AFNEPGSRLNSTTRLILLDNASRNEASKIFGTIENTPISSITMGVSTILGAKKVYLLAWG
EECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEC
ENKAAMIKECVEGPISDTIPASYLQTHNNAHVAIDLSASMNLTRIQRPWLVTSCEWNDKL
CCCHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEEECCCCCHHEECCCEEEEECCCCHHH
IRSAIVWLCQLTGKPILKLTNKDYNENGLSELLALFGSAYNVNIKIFNDLQHTITGWPGG
HHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEECCHHHHHCCCCCC
KPNADDTYRPERAKPYPKRVVIFSPHPDDDVISMGGTLRRLVEQKHEVHVAYETSGNIAV
CCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCHHHHHHHHHHCCEEEEEECCCCEEE
GDEEVVRFMHFINGFNQIFNNSEDLVISEKYAEIRKFLKEKKDGDMDSRDILTIKGLIRR
CHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
GEARTASSYNNIPLDRVHFLDLPFYETGKIQKNPISEADVEIVRNLLREIKPHQIFVAGD
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEC
LADPHGTHRVCTDAVFAAVDLEKEEGAKWLKDCRIWMYRGAWAEWEIENIEMAVPISPEE
CCCCCCCHHHHHHHHHHHEECCHHHHHHHHHHHHEEEEECCCCEEEECCEEEEECCCHHH
LRAKRNSILKHQSQMESAPFLGNDERLFWQRSEDRNRGTATLYDQLGLASYEAMEAFVEY
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEHHHHHCCHHHHHHHHHHHH
IPL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glucosamine 6-phosphate; H2O

Specific reaction: D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3

General reaction: Group transfer (transmolecular Intramolecular oxidoreduction) [C]

Inhibitor: N3-(4-Methoxyfumaroyl)-L-2, 3-diaminopropanoic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928