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The map label for this gene is metH [H]

Identifier: 29345659

GI number: 29345659

Start: 274551

End: 275453

Strand: Reverse

Name: metH [H]

Synonym: BT_0249

Alternate gene names: 29345659

Gene position: 275453-274551 (Counterclockwise)

Preceding gene: 29345660

Following gene: 29345658

Centisome position: 4.4

GC content: 48.28

Gene sequence:

>903_bases
ATGATTTTATCTTATAAGATACATAACGTCGCCCCCTACATCAATTGGATTTACTTTTTCCATGCATGGGGATTCCAACC
TCGTTTCGCAGCGATCGCCAACATTCACGGTTGCGATGTCTGTCGTGCCTCGTGGCTGACTACTTTTCCGGAAGAAGACC
GCAACAAGGCTTCCGAAGCTATGCAACTGTTTAAGGAAGCCAACCGGATGCTGGATTTACTTGACAAGGATTATGAAGTG
AGAACTATCTTCAAACTTTGCAAAGCAAACTCGGACGGAGATAACCTGGTGATTGAAAAAGAAACCGATCAATTCCTAGT
TTTCCCTTTGCTCCGCCAGCAGACGCCTAAACGGGACGGAAGTCCGTTCTTTTGTCTGAGTGACTTTATCCGTCCTCTTT
CTTCCGGCATTCCGGATACAATAGGTGCCTTTGCTTCTTGCATAGATGCAGATATGGAAGGCCTGTACGAGCAAGATCCC
TACAAGCATTTACTCGTCCAGACTCTTTCCGACCGTCTGGCAGAAGCTGCTACGGAAAAGATGCACGAATATGTACGCAA
AGAAGCGTGGGGATACGCCAAAGATGAAATTCTGTCAATGCCCGATCTGTTGGTCGAGAAATATCAAGGGATTCGCCCTG
CCGTAGGCTACCCGTCCCTGCCCGATCAATCCATCAATTTCCTGTTGGACGAACTGCTGGATATGAAACAGATAGGAATC
ACGCTGACCGAGAATGGTGCCATGTACCCTCATGCTTCGGTCTGCGGACTGATGTTTGCCCATCCGGCTGCCGAATACTT
CTCTGTCGGAAAGATAGGAGAAGACCAGCTCGAAGACTATGCCCGCCGTCGGGGAAAAAGTACGGAGGAAATGCGAAAAT
TCCTGGCTGCCAATCTGCAATAA

Upstream 100 bases:

>100_bases
GTGAACGGTCATCTGTTATATAACAACGGAACCATTGACGAGAATTATCGTGGACAGGAGTTAAGATTCAGATAAACAGC
AACTCGTCACTTATAAACAT

Downstream 100 bases:

>100_bases
CTTTCGTTTACTTGTGATTTTTCTCCTTTTCGCACGACTAACTAACCAGAAAAGTAACCCATTTAATTCATGAGAGAACG
AACAATAGAGGCAACCAACC

Product: 5-methyltetrahydrofolate--homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MILSYKIHNVAPYINWIYFFHAWGFQPRFAAIANIHGCDVCRASWLTTFPEEDRNKASEAMQLFKEANRMLDLLDKDYEV
RTIFKLCKANSDGDNLVIEKETDQFLVFPLLRQQTPKRDGSPFFCLSDFIRPLSSGIPDTIGAFASCIDADMEGLYEQDP
YKHLLVQTLSDRLAEAATEKMHEYVRKEAWGYAKDEILSMPDLLVEKYQGIRPAVGYPSLPDQSINFLLDELLDMKQIGI
TLTENGAMYPHASVCGLMFAHPAAEYFSVGKIGEDQLEDYARRRGKSTEEMRKFLAANLQ

Sequences:

>Translated_300_residues
MILSYKIHNVAPYINWIYFFHAWGFQPRFAAIANIHGCDVCRASWLTTFPEEDRNKASEAMQLFKEANRMLDLLDKDYEV
RTIFKLCKANSDGDNLVIEKETDQFLVFPLLRQQTPKRDGSPFFCLSDFIRPLSSGIPDTIGAFASCIDADMEGLYEQDP
YKHLLVQTLSDRLAEAATEKMHEYVRKEAWGYAKDEILSMPDLLVEKYQGIRPAVGYPSLPDQSINFLLDELLDMKQIGI
TLTENGAMYPHASVCGLMFAHPAAEYFSVGKIGEDQLEDYARRRGKSTEEMRKFLAANLQ
>Mature_300_residues
MILSYKIHNVAPYINWIYFFHAWGFQPRFAAIANIHGCDVCRASWLTTFPEEDRNKASEAMQLFKEANRMLDLLDKDYEV
RTIFKLCKANSDGDNLVIEKETDQFLVFPLLRQQTPKRDGSPFFCLSDFIRPLSSGIPDTIGAFASCIDADMEGLYEQDP
YKHLLVQTLSDRLAEAATEKMHEYVRKEAWGYAKDEILSMPDLLVEKYQGIRPAVGYPSLPDQSINFLLDELLDMKQIGI
TLTENGAMYPHASVCGLMFAHPAAEYFSVGKIGEDQLEDYARRRGKSTEEMRKFLAANLQ

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=309, Percent_Identity=36.5695792880259, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI1790450, Length=304, Percent_Identity=41.1184210526316, Blast_Score=200, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI17535405, Length=303, Percent_Identity=38.9438943894389, Blast_Score=192, Evalue=2e-49,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 34279; Mature: 34279

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50974 ADOMET_ACTIVATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSYKIHNVAPYINWIYFFHAWGFQPRFAAIANIHGCDVCRASWLTTFPEEDRNKASEA
CEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHH
MQLFKEANRMLDLLDKDYEVRTIFKLCKANSDGDNLVIEKETDQFLVFPLLRQQTPKRDG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHCCCCCCC
SPFFCLSDFIRPLSSGIPDTIGAFASCIDADMEGLYEQDPYKHLLVQTLSDRLAEAATEK
CCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MHEYVRKEAWGYAKDEILSMPDLLVEKYQGIRPAVGYPSLPDQSINFLLDELLDMKQIGI
HHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCE
TLTENGAMYPHASVCGLMFAHPAAEYFSVGKIGEDQLEDYARRRGKSTEEMRKFLAANLQ
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MILSYKIHNVAPYINWIYFFHAWGFQPRFAAIANIHGCDVCRASWLTTFPEEDRNKASEA
CEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHH
MQLFKEANRMLDLLDKDYEVRTIFKLCKANSDGDNLVIEKETDQFLVFPLLRQQTPKRDG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHCCCCCCC
SPFFCLSDFIRPLSSGIPDTIGAFASCIDADMEGLYEQDPYKHLLVQTLSDRLAEAATEK
CCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MHEYVRKEAWGYAKDEILSMPDLLVEKYQGIRPAVGYPSLPDQSINFLLDELLDMKQIGI
HHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCE
TLTENGAMYPHASVCGLMFAHPAAEYFSVGKIGEDQLEDYARRRGKSTEEMRKFLAANLQ
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185137; 2668277; 8369296; 8265357; 9278503; 1593636; 7992050; 8939751 [H]