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The map label for this gene is nadE [H]

Identifier: 29345615

GI number: 29345615

Start: 220121

End: 222046

Strand: Reverse

Name: nadE [H]

Synonym: BT_0205

Alternate gene names: 29345615

Gene position: 222046-220121 (Counterclockwise)

Preceding gene: 29345628

Following gene: 29345597

Centisome position: 3.55

GC content: 43.77

Gene sequence:

>1926_bases
ATGAACTACGGATTCGTAAAAGTAGCTGCAGCCGTGCCACACGTTAAAGTGGCCGACTGTAAATTCAATGTAGAGAGAAT
AGAAAGCCAAATCGCAATAGCTGAAGGTAAGGGTGTGCAAATCATAGTATTCCCGGAAATGAGTATTACCGGATATACTT
GCGGTGACTTATTCGGACAGCAGATTTTGCTGGAAGAAGCAGAGATGGGGCTAATGCAGATACTCAATAATACCCGCCAA
CTGGACATCATCTCTATTGTCGGCATGCCGGTAGTAGTCAATTCAACCGTGATCAATGCCGCCGTAGTGATTCAAAAAGG
AAAAGTACTAGGTGTTGCTGCCAAAACTTACCTCCCCAATTATAAGGAGTTCTACGAACAGCGCTGGTTTACTTCTGCCC
TTCAACTGACAGAAGATACCGTACGCCTATGCGGACAAATCGTTCCCATAGGTGCCAACCTGCTGTTTGAAACTTCCGAT
ACGACTTTTGGGATCGAAATTTGCGAAGACCTTTGGGCTACCATACCTCCCAGTTCCTCATTGGCTTTACAAGGAGCAGA
AATTATTTTCAATATGTCTGCCGATAATGAAGGAATCGGAAAGCATCATTATCTCTGTTCTCTTATCAGTCAACAATCAG
CACGTTGCATTGCAGGATACGTATTTTCTTCCTGTGGTTTCGGGGAGTCTACTACGGATGTAGTCTTTGCGGGAAACGGA
TTGATTTACGAAAACGGCTCATTACTGGCACGTAGCAAACGTTTCTGCATGGAGGAGCAACTGATCATTAGCGAAATCGA
CGTGGAACGCATACGGGCAGAACGTAGAATTAACACCACTTTTGCTGCCAGCCAGGGAAATCCAGGAGATAAAAAAGCAA
TCTCCGTCGCAACGGAGTTTGTTAATAGCAAAGAGCTTACTTTGACCCGAGACTTCAATTCACATCCTTTTGTCCCCCAA
GGAGCAGAACTGGATGAGCATTGTGAAGAAGTATTCTCTATCCAAATAGCCGGCTTGGCACAACGACTGGTTCATACCAA
AGCTAAAACAGCTGTAGTCGGTATTTCCGGCGGATTGGATTCAACACTGGCATTATTAGTCTGCGTGAAGACCTTCGACA
AACTCGGTTTACCCCGCAAAGATATTCTGGGAGTTACAATGCCAGGCTTTGGAACGACTGACCGTACTTATAACAATGCC
ATTGATTTAATGAAATCATTGGGCATTTCTATCCGGGAAATCAGTATTCAAGATGCCTGTATCCAGCATTTCAAAGATAT
CGATCATGATATCAATGTACATGATGTCACCTATGAGAACTCTCAGGCACGCGAACGCACACAGATATTAATGGATATCG
CTAACCAAACTTGGGGAATGGTAGTGGGTACGGGTGACTTGTCAGAACTTGCACTCGGATGGGCTACGTACAATGGAGAT
CACATGTCTATGTACGGTGTCAATGGAAGTATTCCCAAAACACTTGTGAAGTATCTAGTGCAATGGGTAGCGGAAAATGA
TATGGATGAGGACGCTAAAGCGACTTTACTGGATATTGTAGATACTCCTATCAGTCCCGAATTAATTCCAGCTGACGAAA
ACGGGGAAATCAAACAGAAGACCGAAGACTTGGTAGGGCCTTATGAACTGCATGACTTTTTCCTCTATTACTTCCTGCGT
TTTGGTTTCCGTCCTTCCAAGATTTACTATCTGGCTAATATAGCTTTCAAGGATGTATATGACAAAGAAACAATAAAGAA
ATGGCTATCTACTTTCTTCCGACGTTTCTTTAATCAGCAGTTCAAGCGCTCCTGTCTGCCCGATGGTCCGAAGGTAGGTA
GTATTTCCATCAGTCCCAGAGGTGACTGGCGAATGCCAAGTGATGCAAGTTCAACTATCTGGCTTAAAGAAATAGAAGAT
TTATAA

Upstream 100 bases:

>100_bases
AACGACATAAGACACCTCATTTTGAGGATTCAGAGTCAAAAGGGCAAAAATCCAAAACTATTTCCTATTTTTGTACCCAC
TATATATAAAAAGGTACAAG

Downstream 100 bases:

>100_bases
TCTCTTTGATCTTTCTTCTGTGACATTTTTGTATTTATCTTAAGTATTATACGGGGTAAAACAGAAATGTCACATAACTG
TGTATCAATCATACAGAACA

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MNYGFVKVAAAVPHVKVADCKFNVERIESQIAIAEGKGVQIIVFPEMSITGYTCGDLFGQQILLEEAEMGLMQILNNTRQ
LDIISIVGMPVVVNSTVINAAVVIQKGKVLGVAAKTYLPNYKEFYEQRWFTSALQLTEDTVRLCGQIVPIGANLLFETSD
TTFGIEICEDLWATIPPSSSLALQGAEIIFNMSADNEGIGKHHYLCSLISQQSARCIAGYVFSSCGFGESTTDVVFAGNG
LIYENGSLLARSKRFCMEEQLIISEIDVERIRAERRINTTFAASQGNPGDKKAISVATEFVNSKELTLTRDFNSHPFVPQ
GAELDEHCEEVFSIQIAGLAQRLVHTKAKTAVVGISGGLDSTLALLVCVKTFDKLGLPRKDILGVTMPGFGTTDRTYNNA
IDLMKSLGISIREISIQDACIQHFKDIDHDINVHDVTYENSQARERTQILMDIANQTWGMVVGTGDLSELALGWATYNGD
HMSMYGVNGSIPKTLVKYLVQWVAENDMDEDAKATLLDIVDTPISPELIPADENGEIKQKTEDLVGPYELHDFFLYYFLR
FGFRPSKIYYLANIAFKDVYDKETIKKWLSTFFRRFFNQQFKRSCLPDGPKVGSISISPRGDWRMPSDASSTIWLKEIED
L

Sequences:

>Translated_641_residues
MNYGFVKVAAAVPHVKVADCKFNVERIESQIAIAEGKGVQIIVFPEMSITGYTCGDLFGQQILLEEAEMGLMQILNNTRQ
LDIISIVGMPVVVNSTVINAAVVIQKGKVLGVAAKTYLPNYKEFYEQRWFTSALQLTEDTVRLCGQIVPIGANLLFETSD
TTFGIEICEDLWATIPPSSSLALQGAEIIFNMSADNEGIGKHHYLCSLISQQSARCIAGYVFSSCGFGESTTDVVFAGNG
LIYENGSLLARSKRFCMEEQLIISEIDVERIRAERRINTTFAASQGNPGDKKAISVATEFVNSKELTLTRDFNSHPFVPQ
GAELDEHCEEVFSIQIAGLAQRLVHTKAKTAVVGISGGLDSTLALLVCVKTFDKLGLPRKDILGVTMPGFGTTDRTYNNA
IDLMKSLGISIREISIQDACIQHFKDIDHDINVHDVTYENSQARERTQILMDIANQTWGMVVGTGDLSELALGWATYNGD
HMSMYGVNGSIPKTLVKYLVQWVAENDMDEDAKATLLDIVDTPISPELIPADENGEIKQKTEDLVGPYELHDFFLYYFLR
FGFRPSKIYYLANIAFKDVYDKETIKKWLSTFFRRFFNQQFKRSCLPDGPKVGSISISPRGDWRMPSDASSTIWLKEIED
L
>Mature_641_residues
MNYGFVKVAAAVPHVKVADCKFNVERIESQIAIAEGKGVQIIVFPEMSITGYTCGDLFGQQILLEEAEMGLMQILNNTRQ
LDIISIVGMPVVVNSTVINAAVVIQKGKVLGVAAKTYLPNYKEFYEQRWFTSALQLTEDTVRLCGQIVPIGANLLFETSD
TTFGIEICEDLWATIPPSSSLALQGAEIIFNMSADNEGIGKHHYLCSLISQQSARCIAGYVFSSCGFGESTTDVVFAGNG
LIYENGSLLARSKRFCMEEQLIISEIDVERIRAERRINTTFAASQGNPGDKKAISVATEFVNSKELTLTRDFNSHPFVPQ
GAELDEHCEEVFSIQIAGLAQRLVHTKAKTAVVGISGGLDSTLALLVCVKTFDKLGLPRKDILGVTMPGFGTTDRTYNNA
IDLMKSLGISIREISIQDACIQHFKDIDHDINVHDVTYENSQARERTQILMDIANQTWGMVVGTGDLSELALGWATYNGD
HMSMYGVNGSIPKTLVKYLVQWVAENDMDEDAKATLLDIVDTPISPELIPADENGEIKQKTEDLVGPYELHDFFLYYFLR
FGFRPSKIYYLANIAFKDVYDKETIKKWLSTFFRRFFNQQFKRSCLPDGPKVGSISISPRGDWRMPSDASSTIWLKEIED
L

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=304, Percent_Identity=28.2894736842105, Blast_Score=110, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17538756, Length=295, Percent_Identity=25.4237288135593, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321865, Length=268, Percent_Identity=29.1044776119403, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24641841, Length=682, Percent_Identity=25.0733137829912, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24641843, Length=682, Percent_Identity=25.0733137829912, Blast_Score=125, Evalue=8e-29,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 71231; Mature: 71231

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYGFVKVAAAVPHVKVADCKFNVERIESQIAIAEGKGVQIIVFPEMSITGYTCGDLFGQ
CCCCEEEEEHHCCCEEEECCCCCHHHHHHHEEEECCCCEEEEEEECCCCCCEEHHHHHHH
QILLEEAEMGLMQILNNTRQLDIISIVGMPVVVNSTVINAAVVIQKGKVLGVAAKTYLPN
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCHHEEEEEEEECCCEEEEEHHHHCCC
YKEFYEQRWFTSALQLTEDTVRLCGQIVPIGANLLFETSDTTFGIEICEDLWATIPPSSS
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEECCCCEEHHHHHHHHHHCCCCCCC
LALQGAEIIFNMSADNEGIGKHHYLCSLISQQSARCIAGYVFSSCGFGESTTDVVFAGNG
EEEECEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCE
LIYENGSLLARSKRFCMEEQLIISEIDVERIRAERRINTTFAASQGNPGDKKAISVATEF
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
VNSKELTLTRDFNSHPFVPQGAELDEHCEEVFSIQIAGLAQRLVHTKAKTAVVGISGGLD
CCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCH
STLALLVCVKTFDKLGLPRKDILGVTMPGFGTTDRTYNNAIDLMKSLGISIREISIQDAC
HHHHHHHHHHHHHHHCCCHHHCCEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHH
IQHFKDIDHDINVHDVTYENSQARERTQILMDIANQTWGMVVGTGDLSELALGWATYNGD
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHEEEEEECCC
HMSMYGVNGSIPKTLVKYLVQWVAENDMDEDAKATLLDIVDTPISPELIPADENGEIKQK
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCHHHH
TEDLVGPYELHDFFLYYFLRFGFRPSKIYYLANIAFKDVYDKETIKKWLSTFFRRFFNQQ
HHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHH
FKRSCLPDGPKVGSISISPRGDWRMPSDASSTIWLKEIEDL
HHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEEEHHHHCCC
>Mature Secondary Structure
MNYGFVKVAAAVPHVKVADCKFNVERIESQIAIAEGKGVQIIVFPEMSITGYTCGDLFGQ
CCCCEEEEEHHCCCEEEECCCCCHHHHHHHEEEECCCCEEEEEEECCCCCCEEHHHHHHH
QILLEEAEMGLMQILNNTRQLDIISIVGMPVVVNSTVINAAVVIQKGKVLGVAAKTYLPN
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCHHEEEEEEEECCCEEEEEHHHHCCC
YKEFYEQRWFTSALQLTEDTVRLCGQIVPIGANLLFETSDTTFGIEICEDLWATIPPSSS
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEECCCCEEHHHHHHHHHHCCCCCCC
LALQGAEIIFNMSADNEGIGKHHYLCSLISQQSARCIAGYVFSSCGFGESTTDVVFAGNG
EEEECEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCE
LIYENGSLLARSKRFCMEEQLIISEIDVERIRAERRINTTFAASQGNPGDKKAISVATEF
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
VNSKELTLTRDFNSHPFVPQGAELDEHCEEVFSIQIAGLAQRLVHTKAKTAVVGISGGLD
CCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCH
STLALLVCVKTFDKLGLPRKDILGVTMPGFGTTDRTYNNAIDLMKSLGISIREISIQDAC
HHHHHHHHHHHHHHHCCCHHHCCEEECCCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHH
IQHFKDIDHDINVHDVTYENSQARERTQILMDIANQTWGMVVGTGDLSELALGWATYNGD
HHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHEEEEEECCC
HMSMYGVNGSIPKTLVKYLVQWVAENDMDEDAKATLLDIVDTPISPELIPADENGEIKQK
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCHHHH
TEDLVGPYELHDFFLYYFLRFGFRPSKIYYLANIAFKDVYDKETIKKWLSTFFRRFFNQQ
HHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHH
FKRSCLPDGPKVGSISISPRGDWRMPSDASSTIWLKEIEDL
HHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]