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The map label for this gene is radA [H]

Identifier: 29144848

GI number: 29144848

Start: 4768994

End: 4770376

Strand: Direct

Name: radA [H]

Synonym: t4618

Alternate gene names: 29144848

Gene position: 4768994-4770376 (Clockwise)

Preceding gene: 29144847

Following gene: 29144849

Centisome position: 99.52

GC content: 59.73

Gene sequence:

>1383_bases
GTGGCAAAAGCTCCCAAACGCGCCTTTGTTTGTAATGAATGTGGTGCCGATTACCCACGCTGGCAGGGGCAGTGCAGCGC
CTGCCAGGCCTGGAACACCATTACTGAGGTGCGCCTTGCCGCGTCGCCGACGGTGGCGCGTAACGAACGTCTGAGCGGAT
ACGCCGGCAGTGCGGGCGTATCAAAAGTGCAGAAACTGTCTGACATCAGTCTTGAGGAACTGCCGCGCTTTTCTACCGGT
TTTAAAGAGTTTGACCGCGTGCTGGGCGGCGGGGTGGTACCCGGCAGCGCCATTTTGATTGGCGGTAACCCCGGCGCGGG
CAAATCAACGTTGCTGCTGCAAACGCTGTGCAAACTTGCCGAGCAGATGAAAACGCTGTACGTCACCGGCGAAGAGTCGC
TACAGCAGGTGGCGATGCGCGCGCACCGTCTCGGCCTGCCGACCGCTAATCTGAATATGCTCTCCGAAACCAGCATCGAG
CAGATCTGCCTGATAGCGGAAGAAGAACAGCCGAAGCTAATGGTGATCGACTCCATACAGGTGATGCATATGGCGGACAT
CCAGTCGTCGCCGGGCAGCGTCGCCCAGGTCCGCGAAACGGCAGCCTACCTGACGCGCTTCGCTAAAACGCGCGGCGTGG
CGATCGTGATGGTCGGCCACGTCACCAAAGACGGGTCGCTGGCGGGGCCGAAAGTGCTGGAACACTGTATTGATTGCTCA
GTATTGCTCGACGGCGACGCCGATTCTCGTTTCCGCACGCTGCGCAGCCACAAAAACCGCTTTGGCGCAGTCAATGAGCT
GGGCGTCTTCGCTATGACCGAACAGGGGTTGCGCGAAGTGAGCAATCCGTCCGCCATCTTTTTAAGCCGCGGCGATGAGG
TCACTTCCGGCAGCTCAGTGATGGTCGTCTGGGAAGGTACGCGACCGTTGCTGGTGGAGATTCAGGCACTGGTCGATCAC
TCGATGATGGCGAACCCCAGACGCGTGGCGGTCGGTCTGGAACAAAACCGTCTGGCTATCCTGCTGGCGGTGCTGCACCG
ACACGGCGGTCTGCAAATGTCGGATCAGGACGTCTTCGTCAACGTGGTCGGCGGCGTGAAAGTCACCGAAACCAGCGCGG
ATCTGGCTTTACTGCTGGCGATGGTCTCCAGCCTGCGTGACAGGCCGTTGCCGCAGGATCTGGTGGTGTTTGGCGAAGTG
GGGCTGGCAGGCGAGATTCGTCCGGTGCCGAGCGGGCAGGAGCGTATTTCCGAGGCGGCGAAACACGGTTTTCGTCGGGC
GATTGTGCCTGCCGCCAACGTACCGAAAAAGCCGCCGGAAGGAATGCAGGTGTTCGGTGTTAAAAAGCTCGCAGATGCGT
TAAGCGTGTTTGACGACTTATAA

Upstream 100 bases:

>100_bases
GCATGAACCAGAAATGATTAATCTGCCCAATGGCAAGGCGACCGTAGGCCGGATAAGCGCCAGCGCCATCCGGCAACAGC
GTTAACGAAAGAGGTAAAAC

Downstream 100 bases:

>100_bases
TTCTCTGTATGTAGTGATGTTGTATAATTTGCCGGATGGCGACGCAATGCGTCTTATCCGGCCTACGGATTGATACGGAT
TGATGTTGTAGGCCTGATAA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MAKAPKRAFVCNECGADYPRWQGQCSACQAWNTITEVRLAASPTVARNERLSGYAGSAGVSKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAEQMKTLYVTGEESLQQVAMRAHRLGLPTANLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKPPEGMQVFGVKKLADALSVFDDL

Sequences:

>Translated_460_residues
MAKAPKRAFVCNECGADYPRWQGQCSACQAWNTITEVRLAASPTVARNERLSGYAGSAGVSKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAEQMKTLYVTGEESLQQVAMRAHRLGLPTANLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKPPEGMQVFGVKKLADALSVFDDL
>Mature_459_residues
AKAPKRAFVCNECGADYPRWQGQCSACQAWNTITEVRLAASPTVARNERLSGYAGSAGVSKVQKLSDISLEELPRFSTGF
KEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAEQMKTLYVTGEESLQQVAMRAHRLGLPTANLNMLSETSIEQ
ICLIAEEEQPKLMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCSV
LLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHS
MMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKPPEGMQVFGVKKLADALSVFDDL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=460, Percent_Identity=97.8260869565217, Blast_Score=924, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49433; Mature: 49302

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAPKRAFVCNECGADYPRWQGQCSACQAWNTITEVRLAASPTVARNERLSGYAGSAGV
CCCCCCCCEEEHHCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCHHCCCCCCCCCCCHHH
SKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLA
HHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
EQMKTLYVTGEESLQQVAMRAHRLGLPTANLNMLSETSIEQICLIAEEEQPKLMVIDSIQ
HHHHEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHEEEEECCCCCCEEEEECEE
VMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
EEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHCCCE
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSV
EEEECCCCHHHHHHHHHHHHCCCHHHCCHHEECHHHHHHHCCCCEEEEECCCCCCCCCEE
MVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFV
EEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEE
NVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEVGLAGEIRPVPSGQERISEAA
EEECCEEEECCHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCCCCCHHHHHHHHH
KHGFRRAIVPAANVPKKPPEGMQVFGVKKLADALSVFDDL
HHCHHEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAPKRAFVCNECGADYPRWQGQCSACQAWNTITEVRLAASPTVARNERLSGYAGSAGV
CCCCCCCEEEHHCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCHHCCCCCCCCCCCHHH
SKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLA
HHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
EQMKTLYVTGEESLQQVAMRAHRLGLPTANLNMLSETSIEQICLIAEEEQPKLMVIDSIQ
HHHHEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHEEEEECCCCCCEEEEECEE
VMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
EEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHCCCE
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSV
EEEECCCCHHHHHHHHHHHHCCCHHHCCHHEECHHHHHHHCCCCEEEEECCCCCCCCCEE
MVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFV
EEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEE
NVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEVGLAGEIRPVPSGQERISEAA
EEECCEEEECCHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCCCCCHHHHHHHHH
KHGFRRAIVPAANVPKKPPEGMQVFGVKKLADALSVFDDL
HHCHHEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]