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The map label for this gene is ycdT [H]

Identifier: 29144827

GI number: 29144827

Start: 4748535

End: 4749599

Strand: Reverse

Name: ycdT [H]

Synonym: t4594

Alternate gene names: 29144827

Gene position: 4749599-4748535 (Counterclockwise)

Preceding gene: 29144829

Following gene: 29144826

Centisome position: 99.12

GC content: 49.86

Gene sequence:

>1065_bases
ATGACAACACCATCCTGGCGATCGCTTCGTATTAAGAAGTATCAGTTTTCTTTACGTCTTTTCTTGTTTCTCAGCGCCGT
GTCGGCGCTATTTACTCTCGTTTTCCCCCTTTATCAGATCAATGTATTTTGCACGCCAATGATCGGGATAGTGGGCCTGA
GCGTATTACTGTTGATATGGCATGGGAAATATGGGCAAAAAAGAATTAACCTCCCCTTTATCTCCCTACTCTTTGGGGGA
ATATGGGCGGCGCGTATTGCGCTAAAGTATCCGGCGTTAGGCCATTATGATTTCTCTTTTTTACTGATCTCCCTGCTTAG
CGTTCTGTTTATCGGCTCGATAGCCTTTGCCGCGAATATCGTCGCGTTTACGCTCTATTCGCTACCGCCAGTCGCGGTCT
GCCTGTGGTTGAACGGTAATGAACAAGGTTTGCGAATTCTCTATTTGCTGGCCCTGCCAATGGTGGGAATCGCTATTCAA
CATGTTATCCAGAAACGCTACGATAATTTTGCCCAACAGCTCATGTTTAAGCTGCTGGCCGAGCGGGAAACCCTCAATGG
CCTGAGTATGCTTGATCCATTAACCGGCTTATATAACCGCCGTGGCCTGCAAAATCGGCTGGATACGTTACAGGCGCCGG
GCAGCCATGAACACTACGTCCTGCTATTGGATATCGATCATTTCAAAGCGTATAACGATCACTACGGTCATATGATGGGC
GATCAGGCGTTAATCCGCGTCTCAGCGGCAATTCGGGACGCCGTGCGATCGCGCGATGTCGTCTGTCGTTTTGGCGGGGA
AGAATTTCTGGTGTTGCTGACCACCGCCGAACCACAGCAAGCCCGCGCTACCGCAGAACGTATCCGACAGGAAGTATACG
ATCTTAAAATCCCGCATATGTTTAACGAAAGCGTCGCCACCAATGTCACTGTCAGTATCGGCATTGCGCCCTTGACGGAT
CGCAATATCGGAGACGCTATCGAAAAAGCGGATAAAGCGCTCTACGAAGCCAAGCATCTTGGACGTAACCACATTCTGGT
CAGCGACGACATGCGCGCCCTATGA

Upstream 100 bases:

>100_bases
AAAACAACAACACCTCGCTTTAGGCGACCTTTTCTCATACGTTATTTTCATGTTGGCGCCCGATTCCGTGTATATCACTG
ATAAATAACGACAATTACCC

Downstream 100 bases:

>100_bases
ATCCTGTTGGCGTAATCGTGCTACGCCAACAGCGTTAACCATAAACCGCTTGCCATCGTTTATCGCTATCGATAGGATTA
GCAATCATTATCATTTAGAT

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTTPSWRSLRIKKYQFSLRLFLFLSAVSALFTLVFPLYQINVFCTPMIGIVGLSVLLLIWHGKYGQKRINLPFISLLFGG
IWAARIALKYPALGHYDFSFLLISLLSVLFIGSIAFAANIVAFTLYSLPPVAVCLWLNGNEQGLRILYLLALPMVGIAIQ
HVIQKRYDNFAQQLMFKLLAERETLNGLSMLDPLTGLYNRRGLQNRLDTLQAPGSHEHYVLLLDIDHFKAYNDHYGHMMG
DQALIRVSAAIRDAVRSRDVVCRFGGEEFLVLLTTAEPQQARATAERIRQEVYDLKIPHMFNESVATNVTVSIGIAPLTD
RNIGDAIEKADKALYEAKHLGRNHILVSDDMRAL

Sequences:

>Translated_354_residues
MTTPSWRSLRIKKYQFSLRLFLFLSAVSALFTLVFPLYQINVFCTPMIGIVGLSVLLLIWHGKYGQKRINLPFISLLFGG
IWAARIALKYPALGHYDFSFLLISLLSVLFIGSIAFAANIVAFTLYSLPPVAVCLWLNGNEQGLRILYLLALPMVGIAIQ
HVIQKRYDNFAQQLMFKLLAERETLNGLSMLDPLTGLYNRRGLQNRLDTLQAPGSHEHYVLLLDIDHFKAYNDHYGHMMG
DQALIRVSAAIRDAVRSRDVVCRFGGEEFLVLLTTAEPQQARATAERIRQEVYDLKIPHMFNESVATNVTVSIGIAPLTD
RNIGDAIEKADKALYEAKHLGRNHILVSDDMRAL
>Mature_353_residues
TTPSWRSLRIKKYQFSLRLFLFLSAVSALFTLVFPLYQINVFCTPMIGIVGLSVLLLIWHGKYGQKRINLPFISLLFGGI
WAARIALKYPALGHYDFSFLLISLLSVLFIGSIAFAANIVAFTLYSLPPVAVCLWLNGNEQGLRILYLLALPMVGIAIQH
VIQKRYDNFAQQLMFKLLAERETLNGLSMLDPLTGLYNRRGLQNRLDTLQAPGSHEHYVLLLDIDHFKAYNDHYGHMMGD
QALIRVSAAIRDAVRSRDVVCRFGGEEFLVLLTTAEPQQARATAERIRQEVYDLKIPHMFNESVATNVTVSIGIAPLTDR
NIGDAIEKADKALYEAKHLGRNHILVSDDMRAL

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=42.5925925925926, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786584, Length=182, Percent_Identity=32.4175824175824, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788085, Length=177, Percent_Identity=34.4632768361582, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693134, Length=166, Percent_Identity=40.9638554216867, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=36.5269461077844, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787816, Length=168, Percent_Identity=34.5238095238095, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081881, Length=175, Percent_Identity=35.4285714285714, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=35.7142857142857, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=36.25, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788381, Length=167, Percent_Identity=34.1317365269461, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081974, Length=153, Percent_Identity=32.0261437908497, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788956, Length=175, Percent_Identity=30.2857142857143, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081977, Length=205, Percent_Identity=30.2439024390244, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39921; Mature: 39790

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSWRSLRIKKYQFSLRLFLFLSAVSALFTLVFPLYQINVFCTPMIGIVGLSVLLLIW
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGKYGQKRINLPFISLLFGGIWAARIALKYPALGHYDFSFLLISLLSVLFIGSIAFAANI
CCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VAFTLYSLPPVAVCLWLNGNEQGLRILYLLALPMVGIAIQHVIQKRYDNFAQQLMFKLLA
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERETLNGLSMLDPLTGLYNRRGLQNRLDTLQAPGSHEHYVLLLDIDHFKAYNDHYGHMMG
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEHHHHHCCCCCCCCCC
DQALIRVSAAIRDAVRSRDVVCRFGGEEFLVLLTTAEPQQARATAERIRQEVYDLKIPHM
CHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCH
FNESVATNVTVSIGIAPLTDRNIGDAIEKADKALYEAKHLGRNHILVSDDMRAL
HCCHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHCC
>Mature Secondary Structure 
TTPSWRSLRIKKYQFSLRLFLFLSAVSALFTLVFPLYQINVFCTPMIGIVGLSVLLLIW
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGKYGQKRINLPFISLLFGGIWAARIALKYPALGHYDFSFLLISLLSVLFIGSIAFAANI
CCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VAFTLYSLPPVAVCLWLNGNEQGLRILYLLALPMVGIAIQHVIQKRYDNFAQQLMFKLLA
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERETLNGLSMLDPLTGLYNRRGLQNRLDTLQAPGSHEHYVLLLDIDHFKAYNDHYGHMMG
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEHHHHHCCCCCCCCCC
DQALIRVSAAIRDAVRSRDVVCRFGGEEFLVLLTTAEPQQARATAERIRQEVYDLKIPHM
CHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCH
FNESVATNVTVSIGIAPLTDRNIGDAIEKADKALYEAKHLGRNHILVSDDMRAL
HCCHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]