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The map label for this gene is trmE [H]

Identifier: 29143971

GI number: 29143971

Start: 3786065

End: 3787429

Strand: Reverse

Name: trmE [H]

Synonym: t3677

Alternate gene names: 29143971

Gene position: 3787429-3786065 (Counterclockwise)

Preceding gene: 29143972

Following gene: 29143970

Centisome position: 79.04

GC content: 59.63

Gene sequence:

>1365_bases
ATGAGCCATAACGACACTATCGTCGCCCAGGCAACCCCACCGGGACGCGGTGGCGTGGGTATCCTGCGTATTTCCGGTCT
GAAGGCGCGCGACGTTGCGCAAGAGGTGCTGGGCAAGCTGCCGAAGCCGCGCTATGCCGACTACCTGCCGTTTAAAGATG
TTGACGGCAGCGCGCTGGATCAGGGTATTGCGCTGTGGTTCCCCGGCCCGAACTCCTTCACCGGTGAAGATGTGCTGGAG
CTACAAGGGCACGGCGGTCCGGTGATTCTGGACCTGCTGTTAAAACGTATCCTGACGCTTCCAGGCGTGCGAATTGCCCG
ACCGGGCGAGTTCTCCGAGCGAGCGTTCCTCAACGATAAGCTCGATTTAGCCCAGGCGGAAGCCATTGCCGACCTGATCG
ATGCCAGTTCCGAACAGGCGGCGCGTTCGGCGCTAAACTCGTTACAGGGGGCATTTTCCGCCCGCGTTAACCACCTTGTG
GAAGCACTTACTCACCTGCGAATCTACGTCGAAGCGGCTATTGATTTTCCGGATGAGGAGATCGACTTTCTCTCTGACGG
CAAAATCGAAGCGCAGCTCAATGGCGTCATCGCCGATCTTGACGCCGTGCGCACCGAAGCGCGCCAGGGCAGCCTGCTGC
GCGAAGGGATGAAGGTAGTGATTGCCGGACGCCCGAATGCCGGGAAATCCAGCCTGCTGAATGCGCTGGCAGGCCGTGAA
GCGGCCATTGTCACCGATATCGCCGGTACCACGCGCGACGTGTTGCGCGAGCACATCCACATCGACGGGATGCCGCTGCA
TATCATCGATACCGCCGGACTGCGCGACGCCAACGACGAAGTTGAGCGTATCGGCATCGAACGCGCCTGGCAGGAGATTG
AACAGGCCGATCGAGTGCTGTTTATGGTGGATGGCACCACGACGGACGCCGTTGACCCTGCCGACATCTGGCCGGACTTT
ATCGCCCGTCTGCCCAAAAATCTACCGATTACCGTGGTACGTAACAAGGCGGATATCACCGGCGAAACGCTGGGTATTAG
TGAAGTAAACGGTCACTCACTGGTTCGCCTCTCGGCGCGTACCGGCGAAGGCGTCGACGTGTTGCGTAACCACCTCAAGC
AGAGCATGGGGTTTGATACCAATATGGAAGGCGGCTTCCTGGCCCGCCGCCGTCACCTGCAGGCGCTGGCGGAAGCCGCT
AACCATCTTGAACAGGGCAAAGCGCAGCTATTAGGCGCATGGGCGGGGGAACTGCTGGCGGAAGAGTTGCGCCTGGCGCA
GCAAAGCTTAAGCGAGATTACCGGCGAGTTTACCTCCGACGACCTGCTGGGACGGATTTTCTCCAGCTTCTGTATCGGTA
AATAA

Upstream 100 bases:

>100_bases
GCGCGCCGGTCAGCCTGATGGCGCTAACGCTTATCAGGCCTACAATCAAAAGGCGGTCATATGACCGCCTTTTTTTATTG
CAACAAAGTTGAGACTAACC

Downstream 100 bases:

>100_bases
CGTTGTGTTGCCCGGTGGCGCGATGCTTACCGGGCCTACAAAACCCGTAAGCCGGATAAGGCGTTTACGCCGCCATCCGG
CAGCAATGATTTCCCATGCG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSHNDTIVAQATPPGRGGVGILRISGLKARDVAQEVLGKLPKPRYADYLPFKDVDGSALDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILTLPGVRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNGVIADLDAVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDANDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPADIWPDF
IARLPKNLPITVVRNKADITGETLGISEVNGHSLVRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALAEAA
NHLEQGKAQLLGAWAGELLAEELRLAQQSLSEITGEFTSDDLLGRIFSSFCIGK

Sequences:

>Translated_454_residues
MSHNDTIVAQATPPGRGGVGILRISGLKARDVAQEVLGKLPKPRYADYLPFKDVDGSALDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILTLPGVRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNGVIADLDAVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDANDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPADIWPDF
IARLPKNLPITVVRNKADITGETLGISEVNGHSLVRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALAEAA
NHLEQGKAQLLGAWAGELLAEELRLAQQSLSEITGEFTSDDLLGRIFSSFCIGK
>Mature_453_residues
SHNDTIVAQATPPGRGGVGILRISGLKARDVAQEVLGKLPKPRYADYLPFKDVDGSALDQGIALWFPGPNSFTGEDVLEL
QGHGGPVILDLLLKRILTLPGVRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVE
ALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNGVIADLDAVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGREA
AIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDANDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPADIWPDFI
ARLPKNLPITVVRNKADITGETLGISEVNGHSLVRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALAEAAN
HLEQGKAQLLGAWAGELLAEELRLAQQSLSEITGEFTSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=36.1111111111111, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=33.8, Blast_Score=235, Evalue=7e-62,
Organism=Homo sapiens, GI193082993, Length=462, Percent_Identity=35.7142857142857, Blast_Score=228, Evalue=7e-60,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=94.4933920704846, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=40.6593406593407, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=457, Percent_Identity=28.6652078774617, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6323665, Length=492, Percent_Identity=31.3008130081301, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45550813, Length=483, Percent_Identity=32.2981366459627, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49104; Mature: 48973

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNDTIVAQATPPGRGGVGILRISGLKARDVAQEVLGKLPKPRYADYLPFKDVDGSALD
CCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTLPGVRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCC
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
IDFLSDGKIEAQLNGVIADLDAVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
CCCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDANDEVERIGIERAWQEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDAVDPADIWPDFIARLPKNLPITVVRNKADITGETLGISEVNGHSLVRLSAR
EEECCCCCCCCCCHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEECC
TGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALAEAANHLEQGKAQLLGAWAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQSLSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHNDTIVAQATPPGRGGVGILRISGLKARDVAQEVLGKLPKPRYADYLPFKDVDGSALD
CCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTLPGVRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCC
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
IDFLSDGKIEAQLNGVIADLDAVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
CCCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDANDEVERIGIERAWQEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDAVDPADIWPDFIARLPKNLPITVVRNKADITGETLGISEVNGHSLVRLSAR
EEECCCCCCCCCCHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEECC
TGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALAEAANHLEQGKAQLLGAWAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQSLSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA