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The map label for this gene is priA [H]

Identifier: 29143828

GI number: 29143828

Start: 3625514

End: 3627712

Strand: Direct

Name: priA [H]

Synonym: t3523

Alternate gene names: 29143828

Gene position: 3625514-3627712 (Clockwise)

Preceding gene: 29143826

Following gene: 29143829

Centisome position: 75.66

GC content: 59.35

Gene sequence:

>2199_bases
ATGTCCGTCGCGCACGTTGCCTTGCCCGTTCCGCTTCCCCGAACCTTTGATTACCTGCTGCCGGAAGGCGGGGTCGCCAA
AGCCGGGTGCCGCGTGCGCGTGCCGTTCGGCAAGCAGCAAGAGCGCGTGGGCATTGTCGTCTCGGTTAGCGACCACAGCG
AATTGCCGCTCGATGAATTAAAATCGGTCATTGAAATCCTGGATAACGAGCCAATCTTTTCTCCCTCCATCTGGCGCTTA
CTCCTCTGGGCGGCGGATTACTACCACCATCCCCTCGGCGACGTTCTGTTTCACGCTCTGCCGGTTTTACTGCGTCAGGG
AAAACCAGCCAGCAACGCGCCCCTATGGTATTGGTTCGCCACCGAAGAGGGGCAAGCCGTCGACATCAACAGCCTGAAAC
GCTCCGCCAAACAACAACAGGCGCTGGCGGCGCTGCGGCAAGGAAAGATCTGGCGCTATCAGGTAGCCGAGCTTGATTTT
ACTGACGCCACGCTGCAAACGCTGCGGCGTAAGGGGCTTTGTGAGCTTGCCAGTGAAACGCCCGCGTTTACCGACTGGCG
GGAGCATTATGCCGTCGCAGGAGAGCGCCTGCGGCTAAATACCGAACAGGCGACAGCAGTCGGCGCAATTCACAGCGCGT
CGGACGGTTTCTCCGCCTGGTTGCTGGCAGGCGTAACCGGTTCCGGCAAGACCGAAGTGTATCTGAGCGTGCTGGAAAAC
GTCCTCGCCCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACGCCGCAAACGATCGCCCGTTTTCGCGA
ACGTTTTAACACGCCGGTGGAAGTGCTGCATTCCGGGCTAAACGACAGCGAGCGTCTTTCGGCATGGCTGAAAGCGAAAA
ACGGCGAAGCGGCGATTGTCATCGGCACGCGCTCCTCGCTGTTTACGCCGTTTAAAAATCTTGGCGTAATTGTCATCGAC
GAAGAGCACGACAGCTCTTATAAACAGCAGGAAGGGTGGCGCTACCATGCCCGCGATTTGGCAGTGTATCGCGCCCATAG
CGAGCAGATCCCGATTATTCTCGGCTCCGCCACCCCAGCCCTGGAAACGCTATGCAACGTGCGGCAGAAAAAATACCGGA
TGCTGCGTCTGACGCGGCGCGCAGGCAACGCCCGCCCAGCCCTGCAGCATGTGCTGGATTTAAAAGGACAGCGTCTTCAG
GCGGGACTGGCGCCCGCCCTTATCGCCCGTATGCGCCAACATCTCCAGGCCGATAATCAGGTAATTTTATTTCTTAACCG
CCGGGGATTCGCGCCCGCGCTGCTTTGCCACGACTGCGGCTGGATTGCGGAGTGCCCGCGTTGCGATCACTACTACACGC
TGCACCAGGCGCAGCATCATTTACGCTGCCATCACTGCGACAGCCAGCGTCCGGTACCGCGTCAGTGCCCCTCCTGCGGC
TCCACACATATGCTACCGGTTGGGTTAGGGACCGAACAGCTGGAGCAGGTGCTGGCGCCCTTCTTCCCCGGCGTGCCGAT
CTCACGAATCGACCGTGATACCACCAGTCGTAAAGGCGCGCTGGAGCAGCATCTGGCGGAAGTTCACCGCGGCGGAGCGC
GCATTCTGATAGGCACACAAATGCTGGCGAAAGGCCATCACTTCCCGGACGTCACGCTGGTGGCGTTGCTGGATGTCGAT
GGCGCGCTTTTTTCCGCAGATTTTCGTTCGGCGGAACGCTTCGCCCAGCTCTACACTCAGGTTTCCGGCCGCGCCGGGCG
CGCGGGTAAACAGGGCGAAGTGGTGCTCCAAACGCACCATCCTGAGCATCCCCTGCTGCAAACGTTACTGTATAAAGGCT
ATGATGCGTTTGCGGAACAGGCGCTGGCGGAACGGCAAACGCTCCAGCTTCCGCCGTGGACCAGCCACGTCATTATTCGC
GCAGAGGATCACAACAATCAGCAGGCGCCCGTCTTTCTCCAGCAGTTGCGTAATGTGATTCAGGCCAGCCCGCTATCAGA
CGATAAGCTGTGGATCTTAGGTCCGGTTCCGGCCCTCGCGCCAAAACGCGGCGGCCGCTATCGCTGGCAAATTTTACTTC
AGCATCCTTCACGGATTCTCCTGCAACACATCATCAGCGGCACGCTGGCGTTGATCAATACGCTCCCTGAAGCGCGGAAA
GTTAAATGGGTTCTGGACGTCGATCCGATTGAAGGATAG

Upstream 100 bases:

>100_bases
GGCGAATCATACAGAATTTGACCACCGTATGCAAACTGATCCGCGCTCCCTTATCGTAATGTGTATACTACCCGACGAAA
TTTCAAGTCAGGAAGATGCT

Downstream 100 bases:

>100_bases
TCCCCACGATGCGAGGCGGATCGTAAAATTAAAGATCCATCACACTTTTTATGAAAATTCTGTAACCGCTTCCACGCACT
ATCTGATAAAAATGTTTTCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 732; Mature: 731

Protein sequence:

>732_residues
MSVAHVALPVPLPRTFDYLLPEGGVAKAGCRVRVPFGKQQERVGIVVSVSDHSELPLDELKSVIEILDNEPIFSPSIWRL
LLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFATEEGQAVDINSLKRSAKQQQALAALRQGKIWRYQVAELDF
TDATLQTLRRKGLCELASETPAFTDWREHYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNTPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSSLFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQ
AGLAPALIARMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIR
AEDHNNQQAPVFLQQLRNVIQASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRILLQHIISGTLALINTLPEARK
VKWVLDVDPIEG

Sequences:

>Translated_732_residues
MSVAHVALPVPLPRTFDYLLPEGGVAKAGCRVRVPFGKQQERVGIVVSVSDHSELPLDELKSVIEILDNEPIFSPSIWRL
LLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFATEEGQAVDINSLKRSAKQQQALAALRQGKIWRYQVAELDF
TDATLQTLRRKGLCELASETPAFTDWREHYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNTPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSSLFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQ
AGLAPALIARMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIR
AEDHNNQQAPVFLQQLRNVIQASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRILLQHIISGTLALINTLPEARK
VKWVLDVDPIEG
>Mature_731_residues
SVAHVALPVPLPRTFDYLLPEGGVAKAGCRVRVPFGKQQERVGIVVSVSDHSELPLDELKSVIEILDNEPIFSPSIWRLL
LWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFATEEGQAVDINSLKRSAKQQQALAALRQGKIWRYQVAELDFT
DATLQTLRRKGLCELASETPAFTDWREHYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLENV
LAQGKQALVMVPEIGLTPQTIARFRERFNTPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSSLFTPFKNLGVIVIDE
EHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQA
GLAPALIARMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCGS
THMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDG
ALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIRA
EDHNNQQAPVFLQQLRNVIQASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRILLQHIISGTLALINTLPEARKV
KWVLDVDPIEG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=732, Percent_Identity=90.3005464480874, Blast_Score=1390, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81713; Mature: 81582

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAHVALPVPLPRTFDYLLPEGGVAKAGCRVRVPFGKQQERVGIVVSVSDHSELPLDEL
CCCEEEEECCCCCCCHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCHHHH
KSVIEILDNEPIFSPSIWRLLLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TEEGQAVDINSLKRSAKQQQALAALRQGKIWRYQVAELDFTDATLQTLRRKGLCELASET
CCCCCEECHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHCC
PAFTDWREHYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
CCCHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHH
VLAQGKQALVMVPEIGLTPQTIARFRERFNTPVEVLHSGLNDSERLSAWLKAKNGEAAIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHCCCCCCEEEE
IGTRSSLFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPA
EECCHHHHCHHHHCCEEEEECCCCCCHHHHCCCEEHHHHEEEEECCCCCCCEEEECCCHH
LETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQAGLAPALIARMRQHLQADNQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHCCCCC
VILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
EEEEEECCCCCCHHHHHCCCCHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCC
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQ
CCCEEEECCCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHH
MLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHH
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
PEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIRAEDHNNQQAPVFLQQLRNVI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
QASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRILLQHIISGTLALINTLPEARK
HCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCE
VKWVLDVDPIEG
EEEEEECCCCCC
>Mature Secondary Structure 
SVAHVALPVPLPRTFDYLLPEGGVAKAGCRVRVPFGKQQERVGIVVSVSDHSELPLDEL
CCEEEEECCCCCCCHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCHHHH
KSVIEILDNEPIFSPSIWRLLLWAADYYHHPLGDVLFHALPVLLRQGKPASNAPLWYWFA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TEEGQAVDINSLKRSAKQQQALAALRQGKIWRYQVAELDFTDATLQTLRRKGLCELASET
CCCCCEECHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHCC
PAFTDWREHYAVAGERLRLNTEQATAVGAIHSASDGFSAWLLAGVTGSGKTEVYLSVLEN
CCCHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHH
VLAQGKQALVMVPEIGLTPQTIARFRERFNTPVEVLHSGLNDSERLSAWLKAKNGEAAIV
HHHCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHCCCCCCEEEE
IGTRSSLFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPA
EECCHHHHCHHHHCCEEEEECCCCCCHHHHCCCEEHHHHEEEEECCCCCCCEEEECCCHH
LETLCNVRQKKYRMLRLTRRAGNARPALQHVLDLKGQRLQAGLAPALIARMRQHLQADNQ
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHCCCCC
VILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCG
EEEEEECCCCCCHHHHHCCCCHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCC
STHMLPVGLGTEQLEQVLAPFFPGVPISRIDRDTTSRKGALEQHLAEVHRGGARILIGTQ
CCCEEEECCCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHH
MLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVSGRAGRAGKQGEVVLQTHH
HHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECC
PEHPLLQTLLYKGYDAFAEQALAERQTLQLPPWTSHVIIRAEDHNNQQAPVFLQQLRNVI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
QASPLSDDKLWILGPVPALAPKRGGRYRWQILLQHPSRILLQHIISGTLALINTLPEARK
HCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCE
VKWVLDVDPIEG
EEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]