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The map label for this gene is glyA2

Identifier: 29143820

GI number: 29143820

Start: 3612721

End: 3613980

Strand: Reverse

Name: glyA2

Synonym: t3514

Alternate gene names: 29143820

Gene position: 3613980-3612721 (Counterclockwise)

Preceding gene: 29143821

Following gene: 29143819

Centisome position: 75.42

GC content: 56.59

Gene sequence:

>1260_bases
ATGAATTTTCGGGGGAACCGCATGATAAACAACGATCCGCTTTTTGATTTACTTAATAAAGAGCAACAGCGCCAGCAGCA
CTCGCTGGAACTGATTGCCTCGGAGAATTTCGCCAGCCCTGCCGTTCTTGCGGCACAAGGGTCGGTGTTAACCAATAAAT
ACGCCGAAGGCTATTACCAGCACCGCTACTACGGCGGCTGTAAGTTCATTGATGAGGTTGAGATGCTGGCGATAACCCGC
GCGCAGCAACTTTTCGGCGCGCGTTATGTTAACGTGCAGCCTCACTCCGGCAGCCAGGCGAATCAGGCGGTATATCTGGC
GCTGCTGAAACCCGGCGACAAAATTCTGGGCATGAGCCTGCAATGCGGCGGCCATCTCACTCACGGCTCGCCGGTCAATC
AATCCGGCAAATGGTTTAACGCCTTTCACTATGGCGTCGATGCGCATAGCGGTCTTATTGATATGGATGAGGTTGAGACG
ATCGCGAAGCGTGAGCGCCCGCGGCTGATTATCGCCGGGGGATCGGCCTACCCACGACACTATGATTTTGCCCGCTTTCG
CCGCATTGCCGATGCCGTCGGCGCGATGCTGCTGGTGGATATGGCGCATTTTGCCGGGCTGGTGGCGGGCGGCTGTTTCC
CCTCGCCGCTGGCGTATGCCGATGTGATTACCGCCACCACGCATAAAACGTTGCGCGGGCCGCGTGGCGGCATGATTCTG
ACCAACGACGCCCGGCTGGCGAAAAAAATCGACAGCGCCATCTTTCCCGGCTTGCAGGGCGGGCCGTTGATGCACGTTAT
CGCGGCTAAAGCGGTGGCGCTTGGCGAAGCGCTACAGCCTGAATTTAAACGTTACGCCGGGCAGGTAATCGAAAATGCGC
AAGCGATGTGCCAGCAACTGGCGCAGCGCGGCCTGACGCTGTTAACGGGCGGTACCGACTGCCATCTGGGCATTATCGAC
CTGCGCCCGCAGGGGTTAACCGGCGCTCAGGTGGAATACTTTCTCGAACTGGCGGGGATCACGGTCAATAAAAACACGTT
GCTAGGCGATCCACAGCCGCCGTCGATTACCTCCGGGATTCGCATTGGCAGCGCAGCCTGCGCCACCCGCGGGATGAAGG
CCGATGATTTTACGCTGATCGCGGACTGGATCAGTGAAATTATTTTTGCCATCAAGACGCCGAATATTGCAGATATTTGC
GCCGATATTCGGCAAAAAGTGACTAAATTAACCACCAACTATCCGTTGCCTTACCAATGA

Upstream 100 bases:

>100_bases
CATGTTCCTGCCGAAATTTACCCCTCTATCCGGTGGTCTGCGTCCTGCGTAAAAAGTGGCGGGGTGCGGGGGAAACTTTC
CCTGCGAAAATCATCATATG

Downstream 100 bases:

>100_bases
TGAAACTTGCTGAAATTCAGGCGGCGTGCGGCGTTCTTTGTGTAGATCTCGCCGCTGTCGTTGACAATTATCAAACGCTG
GCGCGCCATGTCGCCCCCGC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT 2; Serine methylase 2

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNFRGNRMINNDPLFDLLNKEQQRQQHSLELIASENFASPAVLAAQGSVLTNKYAEGYYQHRYYGGCKFIDEVEMLAITR
AQQLFGARYVNVQPHSGSQANQAVYLALLKPGDKILGMSLQCGGHLTHGSPVNQSGKWFNAFHYGVDAHSGLIDMDEVET
IAKRERPRLIIAGGSAYPRHYDFARFRRIADAVGAMLLVDMAHFAGLVAGGCFPSPLAYADVITATTHKTLRGPRGGMIL
TNDARLAKKIDSAIFPGLQGGPLMHVIAAKAVALGEALQPEFKRYAGQVIENAQAMCQQLAQRGLTLLTGGTDCHLGIID
LRPQGLTGAQVEYFLELAGITVNKNTLLGDPQPPSITSGIRIGSAACATRGMKADDFTLIADWISEIIFAIKTPNIADIC
ADIRQKVTKLTTNYPLPYQ

Sequences:

>Translated_419_residues
MNFRGNRMINNDPLFDLLNKEQQRQQHSLELIASENFASPAVLAAQGSVLTNKYAEGYYQHRYYGGCKFIDEVEMLAITR
AQQLFGARYVNVQPHSGSQANQAVYLALLKPGDKILGMSLQCGGHLTHGSPVNQSGKWFNAFHYGVDAHSGLIDMDEVET
IAKRERPRLIIAGGSAYPRHYDFARFRRIADAVGAMLLVDMAHFAGLVAGGCFPSPLAYADVITATTHKTLRGPRGGMIL
TNDARLAKKIDSAIFPGLQGGPLMHVIAAKAVALGEALQPEFKRYAGQVIENAQAMCQQLAQRGLTLLTGGTDCHLGIID
LRPQGLTGAQVEYFLELAGITVNKNTLLGDPQPPSITSGIRIGSAACATRGMKADDFTLIADWISEIIFAIKTPNIADIC
ADIRQKVTKLTTNYPLPYQ
>Mature_419_residues
MNFRGNRMINNDPLFDLLNKEQQRQQHSLELIASENFASPAVLAAQGSVLTNKYAEGYYQHRYYGGCKFIDEVEMLAITR
AQQLFGARYVNVQPHSGSQANQAVYLALLKPGDKILGMSLQCGGHLTHGSPVNQSGKWFNAFHYGVDAHSGLIDMDEVET
IAKRERPRLIIAGGSAYPRHYDFARFRRIADAVGAMLLVDMAHFAGLVAGGCFPSPLAYADVITATTHKTLRGPRGGMIL
TNDARLAKKIDSAIFPGLQGGPLMHVIAAKAVALGEALQPEFKRYAGQVIENAQAMCQQLAQRGLTLLTGGTDCHLGIID
LRPQGLTGAQVEYFLELAGITVNKNTLLGDPQPPSITSGIRIGSAACATRGMKADDFTLIADWISEIIFAIKTPNIADIC
ADIRQKVTKLTTNYPLPYQ

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=453, Percent_Identity=44.5916114790287, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862350, Length=453, Percent_Identity=44.5916114790287, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862348, Length=453, Percent_Identity=44.5916114790287, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI19923315, Length=453, Percent_Identity=44.5916114790287, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI261862346, Length=453, Percent_Identity=43.7086092715232, Blast_Score=344, Evalue=7e-95,
Organism=Homo sapiens, GI22547186, Length=394, Percent_Identity=46.4467005076142, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI22547189, Length=380, Percent_Identity=43.9473684210526, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1788902, Length=405, Percent_Identity=54.0740740740741, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25144729, Length=448, Percent_Identity=44.6428571428571, Blast_Score=357, Evalue=6e-99,
Organism=Caenorhabditis elegans, GI25144732, Length=448, Percent_Identity=44.6428571428571, Blast_Score=357, Evalue=7e-99,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=44.8877805486284, Blast_Score=341, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6323087, Length=407, Percent_Identity=43.2432432432432, Blast_Score=331, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24640005, Length=449, Percent_Identity=44.3207126948775, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI221329721, Length=449, Percent_Identity=44.3207126948775, Blast_Score=359, Evalue=2e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA2_SALTI (Q8Z2Z9)

Other databases:

- EMBL:   AL627279
- EMBL:   AE014613
- RefSeq:   NP_457949.1
- RefSeq:   NP_807162.1
- ProteinModelPortal:   Q8Z2Z9
- SMR:   Q8Z2Z9
- GeneID:   1069139
- GeneID:   1250020
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t3514
- KEGG:   sty:STY3764
- HOGENOM:   HBG301263
- OMA:   DICADIR
- ProtClustDB:   PRK13034
- BioCyc:   SENT209261:T3514-MONOMER
- BioCyc:   SENT220341:STY3764-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45559; Mature: 45559

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRGNRMINNDPLFDLLNKEQQRQQHSLELIASENFASPAVLAAQGSVLTNKYAEGYYQ
CCCCCCCCCCCCCHHHHHCHHHHHHHHHHHEEECCCCCCCEEEEECCCHHHHHHHHHHHH
HRYYGGCKFIDEVEMLAITRAQQLFGARYVNVQPHSGSQANQAVYLALLKPGDKILGMSL
HHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEECCCCHHEEEEE
QCGGHLTHGSPVNQSGKWFNAFHYGVDAHSGLIDMDEVETIAKRERPRLIIAGGSAYPRH
ECCCCCCCCCCCCCCCCEEEHEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCC
YDFARFRRIADAVGAMLLVDMAHFAGLVAGGCFPSPLAYADVITATTHKTLRGPRGGMIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
TNDARLAKKIDSAIFPGLQGGPLMHVIAAKAVALGEALQPEFKRYAGQVIENAQAMCQQL
ECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AQRGLTLLTGGTDCHLGIIDLRPQGLTGAQVEYFLELAGITVNKNTLLGDPQPPSITSGI
HHCCCEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCC
RIGSAACATRGMKADDFTLIADWISEIIFAIKTPNIADICADIRQKVTKLTTNYPLPYQ
EECCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNFRGNRMINNDPLFDLLNKEQQRQQHSLELIASENFASPAVLAAQGSVLTNKYAEGYYQ
CCCCCCCCCCCCCHHHHHCHHHHHHHHHHHEEECCCCCCCEEEEECCCHHHHHHHHHHHH
HRYYGGCKFIDEVEMLAITRAQQLFGARYVNVQPHSGSQANQAVYLALLKPGDKILGMSL
HHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEECCCCHHEEEEE
QCGGHLTHGSPVNQSGKWFNAFHYGVDAHSGLIDMDEVETIAKRERPRLIIAGGSAYPRH
ECCCCCCCCCCCCCCCCEEEHEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCC
YDFARFRRIADAVGAMLLVDMAHFAGLVAGGCFPSPLAYADVITATTHKTLRGPRGGMIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
TNDARLAKKIDSAIFPGLQGGPLMHVIAAKAVALGEALQPEFKRYAGQVIENAQAMCQQL
ECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AQRGLTLLTGGTDCHLGIIDLRPQGLTGAQVEYFLELAGITVNKNTLLGDPQPPSITSGI
HHCCCEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCC
RIGSAACATRGMKADDFTLIADWISEIIFAIKTPNIADICADIRQKVTKLTTNYPLPYQ
EECCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504