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The map label for this gene is stbE [H]

Identifier: 29142908

GI number: 29142908

Start: 2601511

End: 2602269

Strand: Direct

Name: stbE [H]

Synonym: t2525

Alternate gene names: 29142908

Gene position: 2601511-2602269 (Clockwise)

Preceding gene: 29142907

Following gene: 29142912

Centisome position: 54.29

GC content: 46.9

Gene sequence:

>759_bases
ATGCGCAATTACAGGCAGTGGTTAGTTTTCAGTAAAGTCATATTAACGCTACTTGGGTTGACGGGATGGTATGGCCCGGC
ACAAGCTGCCGTTAATATTGACAGAACCCGAATTATTTTTGCTTCAGATGAGGTCGCTCAATCGTTGACATTATCCAACG
ATAATACAACGCCGATGCTGTTACAGGTATGGACCGACGCCGGAAACATTGACGCCTCTCCGGATAACAGCAAAACGCCG
CTGGTGGCGCTGCCGCCGGTATTTAAAATGCAGCCAGGAGAACTGAGAACGCTTCGCCTGCTCCTGAGTTCCCGCCAGCA
GCTGGCAACGGACCGGGAGAGTCTCTTCTGGCTAAACATTTATCAGATACCGCCAGTCACCCAGGACATTAAAAACCACC
CGCGAAAACTGGTGTTACCGCTTCGTTTGAGGCTAAAAATTTTAATTCGCCCGACTGGGCTAAAAGCGCCCACGGAAGCG
GAGGAGAAAAAATTACGCTTTATTGCTAAAGAGAATACTATACGGATAGTTAACCCCACCTCGTGGTATATGAGTTTAAC
GTTAACGATGGATAACAAGAAAAGTATCGGCGATATCATGGTTGCGCCGAAAACAGCACTTGATGTGCCGCTAACACGCT
CGCCAACGCCGGGAGCATCGATAAATTATGCAGTAATCAATGATTCCGGGAACTGGCGACAATATACCGCCATACTGGAA
AAATATATTAGTAAGAGTAATTTTATTCCTGAGTTTTGA

Upstream 100 bases:

>100_bases
ACCACACGGGTATCATCCGGTTCAGTAGAAACCTATAGCGGTGATTTTACTGCCTCGCTTGAGGCGCTCGGCGGACAAAC
AGTAACGGCAGGAAGCGTGA

Downstream 100 bases:

>100_bases
TGGGAAATATTCAGGGATACGATCGTATCCCTGAGACAGGCCAGAATTCAGCATGTACCATTGTCAAAAATAGCATTAAG
CTGATATTTTACTTTTTGCA

Product: fimbrial chaperone protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRNYRQWLVFSKVILTLLGLTGWYGPAQAAVNIDRTRIIFASDEVAQSLTLSNDNTTPMLLQVWTDAGNIDASPDNSKTP
LVALPPVFKMQPGELRTLRLLLSSRQQLATDRESLFWLNIYQIPPVTQDIKNHPRKLVLPLRLRLKILIRPTGLKAPTEA
EEKKLRFIAKENTIRIVNPTSWYMSLTLTMDNKKSIGDIMVAPKTALDVPLTRSPTPGASINYAVINDSGNWRQYTAILE
KYISKSNFIPEF

Sequences:

>Translated_252_residues
MRNYRQWLVFSKVILTLLGLTGWYGPAQAAVNIDRTRIIFASDEVAQSLTLSNDNTTPMLLQVWTDAGNIDASPDNSKTP
LVALPPVFKMQPGELRTLRLLLSSRQQLATDRESLFWLNIYQIPPVTQDIKNHPRKLVLPLRLRLKILIRPTGLKAPTEA
EEKKLRFIAKENTIRIVNPTSWYMSLTLTMDNKKSIGDIMVAPKTALDVPLTRSPTPGASINYAVINDSGNWRQYTAILE
KYISKSNFIPEF
>Mature_252_residues
MRNYRQWLVFSKVILTLLGLTGWYGPAQAAVNIDRTRIIFASDEVAQSLTLSNDNTTPMLLQVWTDAGNIDASPDNSKTP
LVALPPVFKMQPGELRTLRLLLSSRQQLATDRESLFWLNIYQIPPVTQDIKNHPRKLVLPLRLRLKILIRPTGLKAPTEA
EEKKLRFIAKENTIRIVNPTSWYMSLTLTMDNKKSIGDIMVAPKTALDVPLTRSPTPGASINYAVINDSGNWRQYTAILE
KYISKSNFIPEF

Specific function: Mediates assembly of pili by forming soluble multimeric complexes with pili subunits as an intermediate step in the assembly process. This protein is involved in type 3 pili assembly [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=228, Percent_Identity=29.8245614035088, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790771, Length=207, Percent_Identity=31.8840579710145, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1789532, Length=223, Percent_Identity=31.8385650224215, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786936, Length=217, Percent_Identity=29.4930875576037, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786743, Length=207, Percent_Identity=28.9855072463768, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787171, Length=216, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788428, Length=236, Percent_Identity=27.1186440677966, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082203, Length=237, Percent_Identity=25.3164556962025, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081806, Length=211, Percent_Identity=28.9099526066351, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788677, Length=192, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 28534; Mature: 28534

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNYRQWLVFSKVILTLLGLTGWYGPAQAAVNIDRTRIIFASDEVAQSLTLSNDNTTPML
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECHHHHHHEEECCCCCCEEE
LQVWTDAGNIDASPDNSKTPLVALPPVFKMQPGELRTLRLLLSSRQQLATDRESLFWLNI
EEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
YQIPPVTQDIKNHPRKLVLPLRLRLKILIRPTGLKAPTEAEEKKLRFIAKENTIRIVNPT
EECCCCHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCHHHHHEEEEEECCEEEEECCC
SWYMSLTLTMDNKKSIGDIMVAPKTALDVPLTRSPTPGASINYAVINDSGNWRQYTAILE
CEEEEEEEEECCCCCCCCEEECCCHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
KYISKSNFIPEF
HHHHHCCCCCCC
>Mature Secondary Structure
MRNYRQWLVFSKVILTLLGLTGWYGPAQAAVNIDRTRIIFASDEVAQSLTLSNDNTTPML
CCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECHHHHHHEEECCCCCCEEE
LQVWTDAGNIDASPDNSKTPLVALPPVFKMQPGELRTLRLLLSSRQQLATDRESLFWLNI
EEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
YQIPPVTQDIKNHPRKLVLPLRLRLKILIRPTGLKAPTEAEEKKLRFIAKENTIRIVNPT
EECCCCHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCHHHHHEEEEEECCEEEEECCC
SWYMSLTLTMDNKKSIGDIMVAPKTALDVPLTRSPTPGASINYAVINDSGNWRQYTAILE
CEEEEEEEEECCCCCCCCEEECCCHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
KYISKSNFIPEF
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1670938 [H]