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The map label for this gene is phnV [H]

Identifier: 29142825

GI number: 29142825

Start: 2508735

End: 2509526

Strand: Direct

Name: phnV [H]

Synonym: t2437

Alternate gene names: 29142825

Gene position: 2508735-2509526 (Clockwise)

Preceding gene: 29142824

Following gene: 29142827

Centisome position: 52.35

GC content: 57.07

Gene sequence:

>792_bases
ATGCTAATTTGGTCACCTAAAGGACGCGCAGCGGCGGGCGTTGTGGCTAGCGTACTGTTTATCGTGTTCTTTTTCCTGCC
GCTGGCGGTGATTCTGATGTCCAGCCTTAGCCAGCGGTGGAACGGTATTCTGCCGAGTGGCTTCACGCTGAACCACTTTG
TTAACGCTCTGCACGGTGCGGCATGGGATGCCCTACTTGCCAGCCTCACGATTGGCTTTTGCGCCAGCCTGTTTGCGCTG
CTTTGCGGCGTTTGGGCGGCGCTGGCGTTGCGTCAATATGGGGTGAAAACGCAGAAATGGTTAAGTATGGTGTTCTATCT
GCCGAGTGCGATTCCGTCAGTGTCCGTGGGCCTGGGCATTCTGGTTGCTTTCAGCCAGGGGCCATTGCAAATGAACGGCA
CCCTATGGATTGTGCTGACAGCGCACTTTGTGCTGATTTCCGCCTTTACCTTCAGTAACGTGTCGACCGGACTGGCGCGC
ATTTCCGCCGATATTGAAAACGTCGCGTCGAGTCTGGGGGCATCGCCCTGGTATCGTCTGCGTCACGTGACGTTGCCGTT
ATTGATGCCGTGGATGGTGTCAGAGCTGGCGCTGAGTTTATCGCTCTCCATGGGGGAGTTGGGCGCGACGGTGATGATTT
ATCCACCGGGCTGGACGACGCTGCCGGTAGCTATCTTTAGCCTGACCGACCGTGGAAATATCGCCGACGGCGCGGCGCTG
ACCATTGTGCTGGTGGCCATTACGCTGCTGCTGATGATGAAGCTGGAGCGCATTGCAAAACGGTTGGGGTAG

Upstream 100 bases:

>100_bases
CGGTCGCCTGCATGATTGCGTTGATTAACATTGTCTTGTCGCTGGGGCTGTTCTCCCTCTATCGCCTGGCGGCATCGCGT
ACCGGCGTAAGGAGCCAGCC

Downstream 100 bases:

>100_bases
AAATAAAACGGTAGGCCGGGTAGGACATAGCCGCCACCCGGCAAAGGAATTACGGGCGATACACCTTCACATTGGCAAAC
CCCTGCTCGCGCAGATAGAG

Product: 2-aminoethylphosphonate transporter membrane protein

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLIWSPKGRAAAGVVASVLFIVFFFLPLAVILMSSLSQRWNGILPSGFTLNHFVNALHGAAWDALLASLTIGFCASLFAL
LCGVWAALALRQYGVKTQKWLSMVFYLPSAIPSVSVGLGILVAFSQGPLQMNGTLWIVLTAHFVLISAFTFSNVSTGLAR
ISADIENVASSLGASPWYRLRHVTLPLLMPWMVSELALSLSLSMGELGATVMIYPPGWTTLPVAIFSLTDRGNIADGAAL
TIVLVAITLLLMMKLERIAKRLG

Sequences:

>Translated_263_residues
MLIWSPKGRAAAGVVASVLFIVFFFLPLAVILMSSLSQRWNGILPSGFTLNHFVNALHGAAWDALLASLTIGFCASLFAL
LCGVWAALALRQYGVKTQKWLSMVFYLPSAIPSVSVGLGILVAFSQGPLQMNGTLWIVLTAHFVLISAFTFSNVSTGLAR
ISADIENVASSLGASPWYRLRHVTLPLLMPWMVSELALSLSLSMGELGATVMIYPPGWTTLPVAIFSLTDRGNIADGAAL
TIVLVAITLLLMMKLERIAKRLG
>Mature_263_residues
MLIWSPKGRAAAGVVASVLFIVFFFLPLAVILMSSLSQRWNGILPSGFTLNHFVNALHGAAWDALLASLTIGFCASLFAL
LCGVWAALALRQYGVKTQKWLSMVFYLPSAIPSVSVGLGILVAFSQGPLQMNGTLWIVLTAHFVLISAFTFSNVSTGLAR
ISADIENVASSLGASPWYRLRHVTLPLLMPWMVSELALSLSLSMGELGATVMIYPPGWTTLPVAIFSLTDRGNIADGAAL
TIVLVAITLLLMMKLERIAKRLG

Specific function: Probably part of the phnSTUV complex (TC 3.A.1.11.5) involved in 2-aminoethylphosphonate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=222, Percent_Identity=26.1261261261261, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017661
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28236; Mature: 28236

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIWSPKGRAAAGVVASVLFIVFFFLPLAVILMSSLSQRWNGILPSGFTLNHFVNALHGA
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AWDALLASLTIGFCASLFALLCGVWAALALRQYGVKTQKWLSMVFYLPSAIPSVSVGLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHH
LVAFSQGPLQMNGTLWIVLTAHFVLISAFTFSNVSTGLARISADIENVASSLGASPWYRL
HEEECCCCEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RHVTLPLLMPWMVSELALSLSLSMGELGATVMIYPPGWTTLPVAIFSLTDRGNIADGAAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEECCCCCHHHHHHHEECCCCCCCCHHHH
TIVLVAITLLLMMKLERIAKRLG
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIWSPKGRAAAGVVASVLFIVFFFLPLAVILMSSLSQRWNGILPSGFTLNHFVNALHGA
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AWDALLASLTIGFCASLFALLCGVWAALALRQYGVKTQKWLSMVFYLPSAIPSVSVGLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHH
LVAFSQGPLQMNGTLWIVLTAHFVLISAFTFSNVSTGLARISADIENVASSLGASPWYRL
HEEECCCCEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RHVTLPLLMPWMVSELALSLSLSMGELGATVMIYPPGWTTLPVAIFSLTDRGNIADGAAL
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEECCCCCHHHHHHHEECCCCCCCCHHHH
TIVLVAITLLLMMKLERIAKRLG
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA