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The map label for this gene is phnU [H]

Identifier: 29142824

GI number: 29142824

Start: 2507872

End: 2508741

Strand: Direct

Name: phnU [H]

Synonym: t2436

Alternate gene names: 29142824

Gene position: 2507872-2508741 (Clockwise)

Preceding gene: 29142823

Following gene: 29142825

Centisome position: 52.33

GC content: 57.24

Gene sequence:

>870_bases
ATGTCACTCATTTTACCTCTGGAAAAACCGGCGCTTAACCTACGCCCGCTGCTGTGGCTGTTGCTCCCGTTGCTGGCGCT
GGCGACGCTGTTTTTCTGGCCTCTGTCGCTCATCGTTGAACAGGCGCTACGCGGTGCCAATGGCGAGATCGGGCTCGAGA
CCTTCCGCCAGGTGGTCGACTCGAAACGCTTTGTCGGCGCGTTGCTGAACACGTTGCAAATCGCCTTTTTCGCCACCGCC
GGTTGCCTGCTGCTGGGCAGCGTGATGTCGCTGATTCTGGTGTTTATTCCTTTCCCCGGCAGCGAGTTGATTGGTCGGGT
CGTCGATACTTTTATTGCGCTGCCGACCTTCCTGATTACGCTGGCGTTCACCTTTATTTATGGGTCAGCGGGTCTGCTCA
ACGGTGCGCTGATGAGCCTGTTTGCCTTTGAGCTACCGCCGGTGGATTTTCTCTACTCGATGCAGGGTGTCATTCTGGCG
GAGATCACGGTGTTCACTCCGCTGGTGATGCGGCCACTGATGGCCGCGCTGCGGCAAATTGATAAAAGCCAGCTAGAGGC
AGCCAGCATTCTGGGGGCACACCCGTTACGGGTGATTGGGCAGGTGATTTTCCCGGCGGCGCTACCGGCGCTGATGGCTA
GTGGCAGCCTGTGCCTGCTGTTGACCACTAACGAGTTCGGCATCGTACTGTTTATTGGCGCGAAAGGGGTTAACACCCTG
CCGATGATGGTTTACAGCAAAGCGATTCTGGAGTCCGACTATACGGTCGCCTGCATGATTGCGTTGATTAACATTGTCTT
GTCGCTGGGGCTGTTCTCCCTCTATCGCCTGGCGGCATCGCGTACCGGCGTAAGGAGCCAGCCATGCTAA

Upstream 100 bases:

>100_bases
TGCGGATGGTGTTGACGCATGTCAATCCGCTGCCGCGCGTGGGCGACAAGCTGGCGCTGTGGTTCGAACCGGATGATGCG
GTCCTGATTGAGGTATAACC

Downstream 100 bases:

>100_bases
TTTGGTCACCTAAAGGACGCGCAGCGGCGGGCGTTGTGGCTAGCGTACTGTTTATCGTGTTCTTTTTCCTGCCGCTGGCG
GTGATTCTGATGTCCAGCCT

Product: 2-aminoethylphosphonate transporter membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSLILPLEKPALNLRPLLWLLLPLLALATLFFWPLSLIVEQALRGANGEIGLETFRQVVDSKRFVGALLNTLQIAFFATA
GCLLLGSVMSLILVFIPFPGSELIGRVVDTFIALPTFLITLAFTFIYGSAGLLNGALMSLFAFELPPVDFLYSMQGVILA
EITVFTPLVMRPLMAALRQIDKSQLEAASILGAHPLRVIGQVIFPAALPALMASGSLCLLLTTNEFGIVLFIGAKGVNTL
PMMVYSKAILESDYTVACMIALINIVLSLGLFSLYRLAASRTGVRSQPC

Sequences:

>Translated_289_residues
MSLILPLEKPALNLRPLLWLLLPLLALATLFFWPLSLIVEQALRGANGEIGLETFRQVVDSKRFVGALLNTLQIAFFATA
GCLLLGSVMSLILVFIPFPGSELIGRVVDTFIALPTFLITLAFTFIYGSAGLLNGALMSLFAFELPPVDFLYSMQGVILA
EITVFTPLVMRPLMAALRQIDKSQLEAASILGAHPLRVIGQVIFPAALPALMASGSLCLLLTTNEFGIVLFIGAKGVNTL
PMMVYSKAILESDYTVACMIALINIVLSLGLFSLYRLAASRTGVRSQPC
>Mature_288_residues
SLILPLEKPALNLRPLLWLLLPLLALATLFFWPLSLIVEQALRGANGEIGLETFRQVVDSKRFVGALLNTLQIAFFATAG
CLLLGSVMSLILVFIPFPGSELIGRVVDTFIALPTFLITLAFTFIYGSAGLLNGALMSLFAFELPPVDFLYSMQGVILAE
ITVFTPLVMRPLMAALRQIDKSQLEAASILGAHPLRVIGQVIFPAALPALMASGSLCLLLTTNEFGIVLFIGAKGVNTLP
MMVYSKAILESDYTVACMIALINIVLSLGLFSLYRLAASRTGVRSQPC

Specific function: Probably part of the phnSTUV complex (TC 3.A.1.11.5) involved in 2-aminoethylphosphonate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=235, Percent_Identity=26.3829787234043, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788764, Length=218, Percent_Identity=27.0642201834862, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017636
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31192; Mature: 31061

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLILPLEKPALNLRPLLWLLLPLLALATLFFWPLSLIVEQALRGANGEIGLETFRQVVD
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SKRFVGALLNTLQIAFFATAGCLLLGSVMSLILVFIPFPGSELIGRVVDTFIALPTFLIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LAFTFIYGSAGLLNGALMSLFAFELPPVDFLYSMQGVILAEITVFTPLVMRPLMAALRQI
HHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKSQLEAASILGAHPLRVIGQVIFPAALPALMASGSLCLLLTTNEFGIVLFIGAKGVNTL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCHH
PMMVYSKAILESDYTVACMIALINIVLSLGLFSLYRLAASRTGVRSQPC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SLILPLEKPALNLRPLLWLLLPLLALATLFFWPLSLIVEQALRGANGEIGLETFRQVVD
CEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SKRFVGALLNTLQIAFFATAGCLLLGSVMSLILVFIPFPGSELIGRVVDTFIALPTFLIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LAFTFIYGSAGLLNGALMSLFAFELPPVDFLYSMQGVILAEITVFTPLVMRPLMAALRQI
HHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DKSQLEAASILGAHPLRVIGQVIFPAALPALMASGSLCLLLTTNEFGIVLFIGAKGVNTL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCHH
PMMVYSKAILESDYTVACMIALINIVLSLGLFSLYRLAASRTGVRSQPC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA