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The map label for this gene is pinE [H]

Identifier: 29141803

GI number: 29141803

Start: 1408218

End: 1408790

Strand: Reverse

Name: pinE [H]

Synonym: t1346

Alternate gene names: 29141803

Gene position: 1408790-1408218 (Counterclockwise)

Preceding gene: 29141805

Following gene: 29141802

Centisome position: 29.4

GC content: 45.03

Gene sequence:

>573_bases
ATGCAAATTGGCTACATTCGTGTGTCAACAAATGACCAGAATACAGATTTACAACGAAACGCACTGAACTGCGCAGGATG
TGAACTGATTTTTGAGGATAAAATCAGCGGAACAAAGTCAGCCCGACCGGGATTGAAAAAGCTGCTCAGAACGTTATCAG
AAGGAGATACGCTCGTTGTCTGGAAGCTGGACAGGCTGGGCAGAAGCATGAAACACCTCATCACTCTTATTGAGGAACTG
CGGGAAAAAGGGGTTAACTTCCGTAGTCTTACAGACAGCATTGATACGTCAACACCCATGGGACGTTTCTTTTTTCACGT
CATGGGGGCTTTAGCGGAAATGGAGCGTGAATTAATAGTTGAACGAACACTGGCCGGACTTGAAGCAGCACGTGCACAGG
GGCGTATTGGTGGACGTCGTCCGAAACTGACAAAAGAGCAACATGAACAAATAGCAAGGCTGATCAAAAATGGCTATAGC
AGGAAACAGTTGGCAATTATTTACGATATCGGTATATCGACGATTTATCGTTATCACCCTGTAGAGGAACTTCAAACTCA
AGCTGAGTTGTAA

Upstream 100 bases:

>100_bases
CCAACGGGCAGTGCAGAGCCTTCCCCCAAACCAACGTTTTCGTCTTAACTCCCACCTATACCAGCTATGTATTTTTAACA
AAACAAAGAGGATATTTTTC

Downstream 100 bases:

>100_bases
TTCTTTTTCTGATATTGAAATGCCGCGGCGATGTAATTTATCTCACGGAAATAGGTACGTTTATCGCGCGGCGCATCAAT
CAGGCTTTCTCGCGGGATGC

Product: DNA-invertase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MQIGYIRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSARPGLKKLLRTLSEGDTLVVWKLDRLGRSMKHLITLIEEL
REKGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTLAGLEAARAQGRIGGRRPKLTKEQHEQIARLIKNGYS
RKQLAIIYDIGISTIYRYHPVEELQTQAEL

Sequences:

>Translated_190_residues
MQIGYIRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSARPGLKKLLRTLSEGDTLVVWKLDRLGRSMKHLITLIEEL
REKGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTLAGLEAARAQGRIGGRRPKLTKEQHEQIARLIKNGYS
RKQLAIIYDIGISTIYRYHPVEELQTQAEL
>Mature_190_residues
MQIGYIRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSARPGLKKLLRTLSEGDTLVVWKLDRLGRSMKHLITLIEEL
REKGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTLAGLEAARAQGRIGGRRPKLTKEQHEQIARLIKNGYS
RKQLAIIYDIGISTIYRYHPVEELQTQAEL

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=181, Percent_Identity=82.8729281767956, Blast_Score=317, Evalue=3e-88,
Organism=Escherichia coli, GI1787827, Length=182, Percent_Identity=38.4615384615385, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1787638, Length=182, Percent_Identity=38.4615384615385, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21602; Mature: 21602

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGYIRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSARPGLKKLLRTLSEGDTLVV
CCEEEEEEECCCCCCCHHHHCCCHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMKHLITLIEELREKGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTLAGLEAARAQGRIGGRRPKLTKEQHEQIARLIKNGYSRKQLAIIYDIGISTIYRYHP
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHCCC
VEELQTQAEL
HHHHHHHCCC
>Mature Secondary Structure
MQIGYIRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSARPGLKKLLRTLSEGDTLVV
CCEEEEEEECCCCCCCHHHHCCCHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMKHLITLIEELREKGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTLAGLEAARAQGRIGGRRPKLTKEQHEQIARLIKNGYSRKQLAIIYDIGISTIYRYHP
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHCCC
VEELQTQAEL
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]