Click here to switch to the map view.

The map label for this gene is rhmD

Identifier: 29141087

GI number: 29141087

Start: 647684

End: 648901

Strand: Direct

Name: rhmD

Synonym: t0572

Alternate gene names: 29141087

Gene position: 647684-648901 (Clockwise)

Preceding gene: 29141086

Following gene: 29141088

Centisome position: 13.52

GC content: 53.53

Gene sequence:

>1218_bases
ATGGAGAATATTATGACTCTTCCTAAAATTAAACACGTTCGAGCCTGGTTTATTGGGGGCGCTACCGCAGAGAAAGGGGC
TGGCGGCGGGGATTATCACGACCAGGGAGGAAATCACTGGATTGATGACCATATCGCCACTCCCATGAGCAAATATCGCG
ACTATGAGCAGTCTCGTCAGTCTTTTGGGATCAACGTGTTAGGGACGCTGATTGTCGAAGTAGAGGCGGAAAATGGGCAG
ACGGGGTTCGCCGTTTCGACAGCAGGGGAGATGGGCTGTTTTATCGTAGAAAAACATCTTAACCGGTTTATTGAAGGCAA
ATGCGTAAGCGATATCAAACTTATTCACGATCAGATGCTCGGCGCGACCATGTACTACTCCGGTTCCGGCGGTCTGGTGA
TGAACACGATTTCCTGTGTGGATCTGGCGCTGTGGGATCTGTTTGGCAAGGTGGTTGGGCTGCCGGTTTATAAATTGCTG
GGCGGAGCGGTACGCGATGAAATTCAGTTTTACGCCACCGGCGCACGCCCGGATCTGGCTAAAGAAATGGGCTTTATCGG
CGGTAAAATGCCGACTCACTGGGGACCGCACGATGGTGATGCGGGGATCCGTAAAGATGCCGCGATGGTGGCGGATATGC
GTGAAAAGTGCGGGCCGGATTTCTGGCTGATGCTCGACTGCTGGATGAGTCAGGATGTGAACTATGCGACTAAACTGGCT
CACGCCTGTGCGCCATTTAATCTCAAATGGATTGAAGAGTGTCTGCCGCCGCAGCAGTATGAAGGCTATCGCGAATTAAA
ACGCAACGCGCCTGCAGGGATGATGGTTACCAGCGGCGAACACCATGGTACGCTGCAATCCTTCCGCACTCTGGCAGAAA
CCGGCATTGATATTATGCAGCCGGATGTTGGCTGGTGCGGCGGATTAACCACGCTGGTTGAGATCGCCGCGCTTGCCAAA
TCGCGCGGGCAACTGGTGGTGCCGCACGGTTCGTCTGTCTATTCGCACCATGCAGTTATTACTTTCACTAATACCCCTTT
CAGCGAGTTCCTGATGACCAGCCCGGACTGCTCCACCCTGCGTCCGCAATTTGACCCGATTCTCCTGGATGAGCCGGTGC
CGGTGAATGGACGCATCCATAAATCAGTGCTGGATAAGCCGGGCTTCGGCGTCGAGCTTAACCGTGACTGTCACTTGAAA
CGCCCTTATAGCCACTAA

Upstream 100 bases:

>100_bases
GAGAATCTTGATTATTTGGCCGGTAAAGCTATTGCCTGTGCCAAAGATATTTCACGCCTGTTGGGATGGAAAAGTCCCTT
CGACTCACTCGCATCTTAAA

Downstream 100 bases:

>100_bases
TAACTCGTGCGTTGCCTTTGTACGGCAACGCCCTCATCCTGTTGAGGATATTGCTATGAGCATCACTTTACTTGACGGCG
TAGTGAAGAAAAACCGCGCA

Product: MR-MLE-family protein

Products: NA

Alternate protein names: RhamD

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MENIMTLPKIKHVRAWFIGGATAEKGAGGGDYHDQGGNHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLIVEVEAENGQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLGATMYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGPDFWLMLDCWMSQDVNYATKLA
HACAPFNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLAETGIDIMQPDVGWCGGLTTLVEIAALAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTLRPQFDPILLDEPVPVNGRIHKSVLDKPGFGVELNRDCHLK
RPYSH

Sequences:

>Translated_405_residues
MENIMTLPKIKHVRAWFIGGATAEKGAGGGDYHDQGGNHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLIVEVEAENGQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLGATMYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGPDFWLMLDCWMSQDVNYATKLA
HACAPFNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLAETGIDIMQPDVGWCGGLTTLVEIAALAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTLRPQFDPILLDEPVPVNGRIHKSVLDKPGFGVELNRDCHLK
RPYSH
>Mature_405_residues
MENIMTLPKIKHVRAWFIGGATAEKGAGGGDYHDQGGNHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLIVEVEAENGQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLGATMYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGPDFWLMLDCWMSQDVNYATKLA
HACAPFNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLAETGIDIMQPDVGWCGGLTTLVEIAALAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTLRPQFDPILLDEPVPVNGRIHKSVLDKPGFGVELNRDCHLK
RPYSH

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR)

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily

Homologues:

Organism=Escherichia coli, GI226510960, Length=401, Percent_Identity=96.2593516209476, Blast_Score=816, Evalue=0.0,
Organism=Escherichia coli, GI48994953, Length=243, Percent_Identity=30.4526748971193, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RHMD_SALCH (Q57M62)

Other databases:

- EMBL:   AE017220
- HSSP:   Q8ZNF9
- ProteinModelPortal:   Q57M62
- SMR:   Q57M62
- GenomeReviews:   AE017220_GR
- KEGG:   sec:SC2294
- HOGENOM:   HBG705576
- OMA:   DEVVPMF
- ProtClustDB:   PRK15440
- BioCyc:   SENT321314:SCH_2294-MONOMER
- HAMAP:   MF_01288
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- PANTHER:   PTHR13794
- SMART:   SM00922

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N

EC number: =4.2.1.90

Molecular weight: Translated: 44608; Mature: 44608

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00908 MR_MLE_1; PS00909 MR_MLE_2

Important sites: ACT_SITE 329-329 BINDING 33-33 BINDING 59-59 BINDING 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIMTLPKIKHVRAWFIGGATAEKGAGGGDYHDQGGNHWIDDHIATPMSKYRDYEQSRQ
CCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
SFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQML
HCCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GATMYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLA
CEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCEEEEECCCCCHHH
KEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGPDFWLMLDCWMSQDVNYATKLA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCHHHHHH
HACAPFNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLAETGIDIMQ
HHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCHHCC
PDVGWCGGLTTLVEIAALAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTL
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEECCEEEEEECCCCHHHHHCCCCCCCCC
RPQFDPILLDEPVPVNGRIHKSVLDKPGFGVELNRDCHLKRPYSH
CCCCCCEEECCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MENIMTLPKIKHVRAWFIGGATAEKGAGGGDYHDQGGNHWIDDHIATPMSKYRDYEQSRQ
CCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
SFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQML
HCCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GATMYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLA
CEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCEEEEECCCCCHHH
KEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGPDFWLMLDCWMSQDVNYATKLA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCHHHHHH
HACAPFNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGEHHGTLQSFRTLAETGIDIMQ
HHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCHHCC
PDVGWCGGLTTLVEIAALAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTL
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEECCEEEEEECCCCHHHHHCCCCCCCCC
RPQFDPILLDEPVPVNGRIHKSVLDKPGFGVELNRDCHLKRPYSH
CCCCCCEEECCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA