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The map label for this gene is fixB

Identifier: 29140619

GI number: 29140619

Start: 89278

End: 90219

Strand: Direct

Name: fixB

Synonym: t0077

Alternate gene names: 29140619

Gene position: 89278-90219 (Clockwise)

Preceding gene: 29140618

Following gene: 29140620

Centisome position: 1.86

GC content: 59.34

Gene sequence:

>942_bases
ATGAACAAATTTTCCAGTGTCTGGGTATTCAGCGATACCCCTTCTCGTCTGCCCGAACTGATGAGTGGTGCGCAGGCTGT
AGGTGAAAAAGTTAACGCGTTCGTACTGAACGAAGCGGACAGCGCGACAGCATGTCATTTGGGCGCGGATCATGTCTGGC
TGCTCAGCGGCAAACCAGAAGATCGCATGATCGAAGACTACGCTGCTGCGATGGCTGAGACTATTCGTCAGCATAGCGAG
GGCGGCGCGGTGCTGCTGCCGAATACGCGTCGGGGCAAGCTGTTGGCGGCAAAACTGGGCTATCGCTTGTCGGCTGCGGT
TTCCAACGACGCCAGCGACGTCTCCCTGCAGGATGGAAAAGCGGCGGTCAAACATATGGTCTACGGCGGTCTGGCGATAG
GGGCAGAGACCATCGCTTCGCCGTTTGCCGTGATCACACTCAGCAGCGGAACGTTTGACGCACAACAGCCGGATGCTTCT
CGCAGCGGCGAGATGCACACCGTGCAGTGGCAGGCTCCGGCCACTGCCGTGACCCGCACCGCAACCCAGGCGCGTCAGAG
CAACAGCGTTGACCTCGACAAAGCGCGTCTGGTGGTCAGCGTGGGACGCGGCATCGGCAGCAAAGAGAATATCTCGCTGG
CCGAAGCGCTGTGCCAGACCATCGGCGCCGAGCTGGCCTGTTCCCGCCCGGTGGCGGAAAACGAGAAGTGGATGGAGCAC
GAGCGCTACGTCGGTATCTCCAACCTGATGCTCAAACCTGAACTGTATCTGGCGGTGGGGATCTCCGGGCAGATCCAACA
CATGGTCGGCGCCAACGGCGCGCAGACGATTTTCGCCATCAACAAAGATAAAAACGCGCCAATCTTCCAGTATGCGGATT
TTGGCATCGTTGGCGATGCGCTGAAGATCCTGCCTGCGCTGACGGCTGCCTTAGCGCGATAA

Upstream 100 bases:

>100_bases
GCGCGAGCGTCAGCGTGTGGTGATTGAAGGTGACGGTGAAGAACAAATCGCCGCGTTTGTCGAGAATCTGCGCAAAATCA
TTTAATTATCAGGGGATGTT

Downstream 100 bases:

>100_bases
ACCATTTCGGGCAGGATACGAAGGTATCCTGCCGCTGACAGGAGTACGTATGTCCGAAGATATCTTTGATGCCATCATCG
TGGGTGCAGGTCTGGCCGGT

Product: electron transfer flavoprotein FixB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNKFSSVWVFSDTPSRLPELMSGAQAVGEKVNAFVLNEADSATACHLGADHVWLLSGKPEDRMIEDYAAAMAETIRQHSE
GGAVLLPNTRRGKLLAAKLGYRLSAAVSNDASDVSLQDGKAAVKHMVYGGLAIGAETIASPFAVITLSSGTFDAQQPDAS
RSGEMHTVQWQAPATAVTRTATQARQSNSVDLDKARLVVSVGRGIGSKENISLAEALCQTIGAELACSRPVAENEKWMEH
ERYVGISNLMLKPELYLAVGISGQIQHMVGANGAQTIFAINKDKNAPIFQYADFGIVGDALKILPALTAALAR

Sequences:

>Translated_313_residues
MNKFSSVWVFSDTPSRLPELMSGAQAVGEKVNAFVLNEADSATACHLGADHVWLLSGKPEDRMIEDYAAAMAETIRQHSE
GGAVLLPNTRRGKLLAAKLGYRLSAAVSNDASDVSLQDGKAAVKHMVYGGLAIGAETIASPFAVITLSSGTFDAQQPDAS
RSGEMHTVQWQAPATAVTRTATQARQSNSVDLDKARLVVSVGRGIGSKENISLAEALCQTIGAELACSRPVAENEKWMEH
ERYVGISNLMLKPELYLAVGISGQIQHMVGANGAQTIFAINKDKNAPIFQYADFGIVGDALKILPALTAALAR
>Mature_313_residues
MNKFSSVWVFSDTPSRLPELMSGAQAVGEKVNAFVLNEADSATACHLGADHVWLLSGKPEDRMIEDYAAAMAETIRQHSE
GGAVLLPNTRRGKLLAAKLGYRLSAAVSNDASDVSLQDGKAAVKHMVYGGLAIGAETIASPFAVITLSSGTFDAQQPDAS
RSGEMHTVQWQAPATAVTRTATQARQSNSVDLDKARLVVSVGRGIGSKENISLAEALCQTIGAELACSRPVAENEKWMEH
ERYVGISNLMLKPELYLAVGISGQIQHMVGANGAQTIFAINKDKNAPIFQYADFGIVGDALKILPALTAALAR

Specific function: Required for anaerobic carnitine reduction. May bring reductant to CaiA

COG id: COG2025

COG function: function code C; Electron transfer flavoprotein, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF alpha-subunit/fixB family

Homologues:

Organism=Homo sapiens, GI189181759, Length=128, Percent_Identity=43.75, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4503607, Length=128, Percent_Identity=43.75, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1786226, Length=313, Percent_Identity=84.3450479233227, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1787990, Length=313, Percent_Identity=45.0479233226837, Blast_Score=280, Evalue=7e-77,
Organism=Escherichia coli, GI87082157, Length=253, Percent_Identity=28.4584980237154, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17506929, Length=225, Percent_Identity=31.5555555555556, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6325261, Length=138, Percent_Identity=34.7826086956522, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136898, Length=225, Percent_Identity=35.5555555555556, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652801, Length=225, Percent_Identity=35.5555555555556, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FIXB_SALA4 (B5F757)

Other databases:

- EMBL:   CP001138
- RefSeq:   YP_002145060.1
- ProteinModelPortal:   B5F757
- SMR:   B5F757
- GeneID:   6795869
- GenomeReviews:   CP001138_GR
- KEGG:   sea:SeAg_B0085
- HOGENOM:   HBG615986
- OMA:   FSDIASR
- ProtClustDB:   PRK03363
- GO:   GO:0006810
- HAMAP:   MF_01056
- InterPro:   IPR001308
- InterPro:   IPR014730
- InterPro:   IPR014731
- InterPro:   IPR018206
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF000089
- SMART:   SM00893

Pfam domain/function: PF01012 ETF; PF00766 ETF_alpha

EC number: NA

Molecular weight: Translated: 33223; Mature: 33223

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00696 ETF_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFSSVWVFSDTPSRLPELMSGAQAVGEKVNAFVLNEADSATACHLGADHVWLLSGKPE
CCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCEEEEECCCCC
DRMIEDYAAAMAETIRQHSEGGAVLLPNTRRGKLLAAKLGYRLSAAVSNDASDVSLQDGK
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEHHCCEEEEEECCCCCCCEECCHH
AAVKHMVYGGLAIGAETIASPFAVITLSSGTFDAQQPDASRSGEMHTVQWQAPATAVTRT
HHHHHHHHCCHHHCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
ATQARQSNSVDLDKARLVVSVGRGIGSKENISLAEALCQTIGAELACSRPVAENEKWMEH
HHHHHCCCCCCHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCHHHHHH
ERYVGISNLMLKPELYLAVGISGQIQHMVGANGAQTIFAINKDKNAPIFQYADFGIVGDA
HHCCCHHHEEECCCEEEEEECCCCCHHHHCCCCCEEEEEEECCCCCCEEEECCCCHHHHH
LKILPALTAALAR
HHHHHHHHHHHCC
>Mature Secondary Structure
MNKFSSVWVFSDTPSRLPELMSGAQAVGEKVNAFVLNEADSATACHLGADHVWLLSGKPE
CCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCEEEEECCCCC
DRMIEDYAAAMAETIRQHSEGGAVLLPNTRRGKLLAAKLGYRLSAAVSNDASDVSLQDGK
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEHHCCEEEEEECCCCCCCEECCHH
AAVKHMVYGGLAIGAETIASPFAVITLSSGTFDAQQPDASRSGEMHTVQWQAPATAVTRT
HHHHHHHHCCHHHCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
ATQARQSNSVDLDKARLVVSVGRGIGSKENISLAEALCQTIGAELACSRPVAENEKWMEH
HHHHHCCCCCCHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCHHHHHH
ERYVGISNLMLKPELYLAVGISGQIQHMVGANGAQTIFAINKDKNAPIFQYADFGIVGDA
HHCCCHHHEEECCCEEEEEECCCCCHHHHCCCCCEEEEEEECCCCCCEEEECCCCHHHHH
LKILPALTAALAR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA