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The map label for this gene is caiT [H]

Identifier: 29140617

GI number: 29140617

Start: 86494

End: 88011

Strand: Reverse

Name: caiT [H]

Synonym: t0075

Alternate gene names: 29140617

Gene position: 88011-86494 (Counterclockwise)

Preceding gene: 29140625

Following gene: 29140616

Centisome position: 1.84

GC content: 53.75

Gene sequence:

>1518_bases
ATGAAAAATGAAAAGAGAAAATCGGGAATTGAACCGAAGGTTTTTTTTCCGCTATTAATAATTGTCGGGATACTTTGCTG
GCTAACAGTCAGAGATCTTGATGCCGCTAACGTCGTTATTAATGCGGTATTCAGTTATGTCACCAACGTCTGGGGTTGGG
CATTTGAATGGTATATGGTCGTCATGCTCTTCAGCTGGTTCTGGCTGGTTTTTGGCCCCTATGCCAAAAAGAGATTAGGT
GACGAAAAACCGGAGTTTAGCACCGCCAGCTGGATTTTCATGATGTTCGCCTCCTGTACATCCGCTGCGGTTCTGTTCTG
GGGCTCTATTGAGATCTACTACTATATCTCCACCCCGCCGTTCGGCCTGGAACCCAATTCCACGGGCGCGAAAGAGATAG
GCCTGGCGTACAGCCTGTTCCACTGGGGGCCGCTACCGTGGGCAACCTATAGCTTCCTTTCCGTGGCCTTCGCCTACTTC
TTCTTTGTACGCAAGATGGATGTCATCCGCCCCAGCTCCACGCTGGTACCGCTCGTCGGTGAAAAGCATGCGAAAGGGTT
GTTCAGTACCATCGTCGATAACTTCTACCTGGTGGCGCTGATCTTCGCGATGGGCACCAGCCTCGGTCTGGCTACCCCGC
TGGTCACCGAGTGTATGCAATGGCTGTTCGGTATTCCGCATACGCTGCAATTGGATGCCATCATCATTACCTGCTGGATC
ATTCTGAACGCGATTTGCGTGGCCTGCGGCCTGCAAAAAGGGGTCAGAATCGCCAGCGATGTGCGTAGCTACCTGAGCTT
CCTGATGCTCGGCTGGGTGTTTATCGTCAGCGGCGCCAGCTTCATCATGAACTACTTCACCGACTCCGTCGGGATGCTGT
TAATGCACCTGCCACGGATGCTGTTCTATACCGACGCCATCGGTAAAGGCGGCTTCCCACAGGGCTGGACCGTTTTCTAC
TGGGCGTGGTGGGTTATTTACGCCATCCAGATGAGTATCTTCCTGGCCCGTATTTCCCGTGGTCGTACCGTACGTGAACT
GTGCTTCGGGATGGTGATGGGTCTGACCGCGTCTACCTGGATCCTGTGGACAGTCCTTGGCAGTAACACGCTGCTGCTGA
TGGATAAAAACATCCTCAACATCCCGCAACTGATTGAGCAACACGGCGTGGCGCGCGCCATTATCGAAACCTGGGCAGCC
TTGCCGCTCAGCACCGCCACCATGTGGGGCTTCTTCATCCTCTGCTTTATTGCCACCGTCACGCTGATTAACGCCTGCTC
TTACACCCTGGCGATGTCGACCTGTCGCGAAGTTCGCGATGGTGAAGAGCCGCCTCTGTTAGTACGCATCGGCTGGTCGG
TGCTGGTCGGCATCATCGGTATCGTTCTGCTGGCGCTGGGTGGGTTGAAGCCGATTCAGACCGCGATTATCGCCGGAGGA
TGCCCGCTGTTCTTCGTCAACATTATGGTGACGCTGTCCTTTATTAAAGACGCCAAAGTGCACTGGAAAGACAAGTAA

Upstream 100 bases:

>100_bases
CAGAGGTTAAAAACTTATCAACAAATCATTAATAATAAATAAACAAACGTTCATGAACTGGCACGCTAAATAAATTTCGT
TTTCACACTGGAATTCACTT

Downstream 100 bases:

>100_bases
TTCTTATTAACCCAATTGATCAAGAGGCTGAAAGATGGATTTCAACTTAAATGACGAACAGGAGCTGTTTGTCGCCGGTA
TTCGCGAACTGATGGCCAGT

Product: L-carnitine/gamma-butyrobetaine antiporter

Products: Proton [Cytoplasm]; L-carnitine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MKNEKRKSGIEPKVFFPLLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMVVMLFSWFWLVFGPYAKKRLG
DEKPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPPFGLEPNSTGAKEIGLAYSLFHWGPLPWATYSFLSVAFAYF
FFVRKMDVIRPSSTLVPLVGEKHAKGLFSTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWI
ILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMHLPRMLFYTDAIGKGGFPQGWTVFY
WAWWVIYAIQMSIFLARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNILNIPQLIEQHGVARAIIETWAA
LPLSTATMWGFFILCFIATVTLINACSYTLAMSTCREVRDGEEPPLLVRIGWSVLVGIIGIVLLALGGLKPIQTAIIAGG
CPLFFVNIMVTLSFIKDAKVHWKDK

Sequences:

>Translated_505_residues
MKNEKRKSGIEPKVFFPLLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMVVMLFSWFWLVFGPYAKKRLG
DEKPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPPFGLEPNSTGAKEIGLAYSLFHWGPLPWATYSFLSVAFAYF
FFVRKMDVIRPSSTLVPLVGEKHAKGLFSTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWI
ILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMHLPRMLFYTDAIGKGGFPQGWTVFY
WAWWVIYAIQMSIFLARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNILNIPQLIEQHGVARAIIETWAA
LPLSTATMWGFFILCFIATVTLINACSYTLAMSTCREVRDGEEPPLLVRIGWSVLVGIIGIVLLALGGLKPIQTAIIAGG
CPLFFVNIMVTLSFIKDAKVHWKDK
>Mature_505_residues
MKNEKRKSGIEPKVFFPLLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMVVMLFSWFWLVFGPYAKKRLG
DEKPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPPFGLEPNSTGAKEIGLAYSLFHWGPLPWATYSFLSVAFAYF
FFVRKMDVIRPSSTLVPLVGEKHAKGLFSTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWI
ILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMHLPRMLFYTDAIGKGGFPQGWTVFY
WAWWVIYAIQMSIFLARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNILNIPQLIEQHGVARAIIETWAA
LPLSTATMWGFFILCFIATVTLINACSYTLAMSTCREVRDGEEPPLLVRIGWSVLVGIIGIVLLALGGLKPIQTAIIAGG
CPLFFVNIMVTLSFIKDAKVHWKDK

Specific function: Catalyzes the exchange of L-carnitine for gamma- butyrobetaine and related betaines [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family. CaiT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786224, Length=504, Percent_Identity=96.031746031746, Blast_Score=983, Evalue=0.0,
Organism=Escherichia coli, GI1788102, Length=438, Percent_Identity=29.2237442922374, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1786506, Length=511, Percent_Identity=25.6360078277886, Blast_Score=155, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56748; Mature: 56748

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNEKRKSGIEPKVFFPLLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMV
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VMLFSWFWLVFGPYAKKRLGDEKPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPP
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCC
FGLEPNSTGAKEIGLAYSLFHWGPLPWATYSFLSVAFAYFFFVRKMDVIRPSSTLVPLVG
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
EKHAKGLFSTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWI
CHHHHHHHHHHHHHHHHHHHHHHHHCHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMHLPRM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFYTDAIGKGGFPQGWTVFYWAWWVIYAIQMSIFLARISRGRTVRELCFGMVMGLTASTW
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILWTVLGSNTLLLMDKNILNIPQLIEQHGVARAIIETWAALPLSTATMWGFFILCFIATV
HHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TLINACSYTLAMSTCREVRDGEEPPLLVRIGWSVLVGIIGIVLLALGGLKPIQTAIIAGG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
CPLFFVNIMVTLSFIKDAKVHWKDK
HHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKNEKRKSGIEPKVFFPLLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMV
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VMLFSWFWLVFGPYAKKRLGDEKPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPP
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCC
FGLEPNSTGAKEIGLAYSLFHWGPLPWATYSFLSVAFAYFFFVRKMDVIRPSSTLVPLVG
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
EKHAKGLFSTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWI
CHHHHHHHHHHHHHHHHHHHHHHHHCHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMHLPRM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFYTDAIGKGGFPQGWTVFYWAWWVIYAIQMSIFLARISRGRTVRELCFGMVMGLTASTW
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILWTVLGSNTLLLMDKNILNIPQLIEQHGVARAIIETWAALPLSTATMWGFFILCFIATV
HHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TLINACSYTLAMSTCREVRDGEEPPLLVRIGWSVLVGIIGIVLLALGGLKPIQTAIIAGG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
CPLFFVNIMVTLSFIKDAKVHWKDK
HHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-carnitine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-carnitine [Periplasm] = Proton [Cytoplasm] + L-carnitine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA