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The map label for this gene is caiA [H]

Identifier: 29140616

GI number: 29140616

Start: 85317

End: 86459

Strand: Reverse

Name: caiA [H]

Synonym: t0074

Alternate gene names: 29140616

Gene position: 86459-85317 (Counterclockwise)

Preceding gene: 29140617

Following gene: 29140615

Centisome position: 1.8

GC content: 52.49

Gene sequence:

>1143_bases
ATGGATTTCAACTTAAATGACGAACAGGAGCTGTTTGTCGCCGGTATTCGCGAACTGATGGCCAGTGAGAACTGGGAAGC
TTATTTTGCCGAGTGCGATCGTGACAGCGTCTACCCTGAGCGTTTTGTAAAAGCGCTGGCGGATATGGGTATTGATAGCC
TGCTGATCCCGGAAGAACACGGCGGTCTGGAAGCGGGTTTTGTTACCGTCGCCGCCGTCTGGATGGAACTGGGACGTCTT
GGCGCGCCAACTTACGTGTTGTACCAATTGCCGGGAGGTTTTAACACTTTCCTGCGTGAAGGGACTCAGGAGCAAATTGA
TAAAATCATGGCGTTCCGGGGCACCGGTAAGCAGATGTGGAACTCTGCTATCACCGAACCGGGAGCGGGATCGGATGTTG
GTAGTCTGAAAACCACTTATACACGCAAAAATGGTAAGGTTTATCTCAATGGCAGTAAGTGCTTTATCACCAGTAGCGCC
TATACCCCGTACATCGTGGTCATGGCGAGAGACGGTGCATCGCCGGATAAACCGGTTTACACCGAGTGGTTTGTGGACAT
GAGCAAAGCGGGTATCAAGGTCAACAAACTGGAGAAACTCGGTCTGCGCATGGATAGCTGCTGTGAAATCACCTTTGACG
ACGTTGAACTGGACGAGAAAGACATGTTCGGTCGGGAAGGTAACGGCTTCAACCGCGTGAAAGAAGAGTTTGACCATGAA
CGCTTCCTCGTCGCGCTGACCAACTACGGTACCGCAATGTGCACTTTTGAAGACGCTGCGCGTTACGCTAATCAGCGCGT
GCAGTTTGGTGAAGCTATTGGCCGTTTCCAGCTGATTCAGGAGAAGTTTGCGCATATGGCGATCAAACTGAACTCCATGA
AAAATATGCTGCTGGAAGCCGCGTGGAAAGCAGATAACGGCACTATTACCTCCGGCGACGCGGCCATGTGCAAATACTTC
TGCGCCAACGCCGCGTTTGAAGTGGTAGACACCGCCATGCAGGTACTGGGAGGTGTTGGTATCGCGGGCAACCACCGCAT
CACGCGTTTCTGGCGTGACCTGCGCGTTGACCGTGTTTCTGGTGGTTCTGACGAAATGCAGATCCTGACGTTGGGTCGTG
CGGTACTCAAGCAATACCGCTAA

Upstream 100 bases:

>100_bases
TTCGTCAACATTATGGTGACGCTGTCCTTTATTAAAGACGCCAAAGTGCACTGGAAAGACAAGTAATTCTTATTAACCCA
ATTGATCAAGAGGCTGAAAG

Downstream 100 bases:

>100_bases
TCGATCCCTTGTAGGCCTGATAAGACGCGGGGCGTCGCCATCAGGCAATCATGCCGGATGGCGCTGTGCTTATCCGGCCT
ACATACCGCAAAAAATGCCA

Product: crotonobetainyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA reductase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEHGGLEAGFVTVAAVWMELGRL
GAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRKNGKVYLNGSKCFITSSA
YTPYIVVMARDGASPDKPVYTEWFVDMSKAGIKVNKLEKLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE
RFLVALTNYGTAMCTFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLLEAAWKADNGTITSGDAAMCKYF
CANAAFEVVDTAMQVLGGVGIAGNHRITRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR

Sequences:

>Translated_380_residues
MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEHGGLEAGFVTVAAVWMELGRL
GAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRKNGKVYLNGSKCFITSSA
YTPYIVVMARDGASPDKPVYTEWFVDMSKAGIKVNKLEKLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE
RFLVALTNYGTAMCTFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLLEAAWKADNGTITSGDAAMCKYF
CANAAFEVVDTAMQVLGGVGIAGNHRITRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR
>Mature_380_residues
MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEHGGLEAGFVTVAAVWMELGRL
GAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRKNGKVYLNGSKCFITSSA
YTPYIVVMARDGASPDKPVYTEWFVDMSKAGIKVNKLEKLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE
RFLVALTNYGTAMCTFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLLEAAWKADNGTITSGDAAMCKYF
CANAAFEVVDTAMQVLGGVGIAGNHRITRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma- butyrobetainyl-CoA [H]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=354, Percent_Identity=29.3785310734463, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI4501859, Length=379, Percent_Identity=26.3852242744063, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI4557231, Length=389, Percent_Identity=27.7634961439589, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI187960098, Length=389, Percent_Identity=27.7634961439589, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI226958412, Length=369, Percent_Identity=28.1842818428184, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI226958414, Length=340, Percent_Identity=28.5294117647059, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI21361497, Length=363, Percent_Identity=30.8539944903581, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI4501857, Length=383, Percent_Identity=26.1096605744125, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI76496475, Length=381, Percent_Identity=29.3963254593176, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4557235, Length=381, Percent_Identity=29.3963254593176, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI7656849, Length=385, Percent_Identity=25.7142857142857, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI4503943, Length=355, Percent_Identity=25.3521126760563, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI7669494, Length=334, Percent_Identity=25.1497005988024, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786223, Length=380, Percent_Identity=97.3684210526316, Blast_Score=776, Evalue=0.0,
Organism=Escherichia coli, GI87081958, Length=382, Percent_Identity=48.1675392670157, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI87082384, Length=329, Percent_Identity=24.9240121580547, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538396, Length=382, Percent_Identity=31.151832460733, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17569725, Length=368, Percent_Identity=28.5326086956522, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17534899, Length=393, Percent_Identity=27.735368956743, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17508101, Length=380, Percent_Identity=25.5263157894737, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17506239, Length=382, Percent_Identity=27.2251308900524, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17570075, Length=389, Percent_Identity=28.2776349614396, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17533517, Length=357, Percent_Identity=27.7310924369748, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71985184, Length=380, Percent_Identity=26.5789473684211, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17505929, Length=361, Percent_Identity=24.6537396121884, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17551932, Length=369, Percent_Identity=24.1192411924119, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71990804, Length=328, Percent_Identity=25.9146341463415, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17534353, Length=278, Percent_Identity=28.7769784172662, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32563615, Length=231, Percent_Identity=31.1688311688312, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71985192, Length=320, Percent_Identity=25.625, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71982178, Length=278, Percent_Identity=22.3021582733813, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562446, Length=336, Percent_Identity=23.8095238095238, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21356377, Length=378, Percent_Identity=28.5714285714286, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24646207, Length=351, Percent_Identity=29.0598290598291, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI21355753, Length=344, Percent_Identity=30.8139534883721, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24666513, Length=378, Percent_Identity=27.5132275132275, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI281363737, Length=351, Percent_Identity=29.9145299145299, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24660351, Length=349, Percent_Identity=27.7936962750716, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19920834, Length=282, Percent_Identity=26.9503546099291, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 42512; Mature: 42512

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00072 ACYL_COA_DH_1 ; PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEH
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEECCCCC
GGLEAGFVTVAAVWMELGRLGAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMW
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCHHHH
NSAITEPGAGSDVGSLKTTYTRKNGKVYLNGSKCFITSSAYTPYIVVMARDGASPDKPVY
HHHCCCCCCCCCCCCCEEEEEECCCEEEECCCEEEEEECCCCCEEEEEEECCCCCCCCCH
TEWFVDMSKAGIKVNKLEKLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE
HHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEECCCCCCHHHCCCCCCCCHHHHHHHCCCC
RFLVALTNYGTAMCTFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLLEA
EEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWKADNGTITSGDAAMCKYFCANAAFEVVDTAMQVLGGVGIAGNHRITRFWRDLRVDRVS
HHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
GGSDEMQILTLGRAVLKQYR
CCCCCEEHHHHHHHHHHHCC
>Mature Secondary Structure
MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEH
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEECCCCC
GGLEAGFVTVAAVWMELGRLGAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMW
CCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCHHHH
NSAITEPGAGSDVGSLKTTYTRKNGKVYLNGSKCFITSSAYTPYIVVMARDGASPDKPVY
HHHCCCCCCCCCCCCCEEEEEECCCEEEECCCEEEEEECCCCCEEEEEEECCCCCCCCCH
TEWFVDMSKAGIKVNKLEKLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE
HHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEECCCCCCHHHCCCCCCCCHHHHHHHCCCC
RFLVALTNYGTAMCTFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLLEA
EEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWKADNGTITSGDAAMCKYFCANAAFEVVDTAMQVLGGVGIAGNHRITRFWRDLRVDRVS
HHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
GGSDEMQILTLGRAVLKQYR
CCCCCEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA