Click here to switch to the map view.

The map label for this gene is yhcR [H]

Identifier: 29140577

GI number: 29140577

Start: 37103

End: 38674

Strand: Reverse

Name: yhcR [H]

Synonym: t0035

Alternate gene names: 29140577

Gene position: 38674-37103 (Counterclockwise)

Preceding gene: 29140578

Following gene: 29140575

Centisome position: 0.81

GC content: 46.69

Gene sequence:

>1572_bases
ATGGTTTATATGAACAAAAAGTTTTCGATATCCCTACTGTCGCTGTGCATTGGTTTGTCTTCAGCCATTTCCTTTTCAGC
CGATGCGCGTGACATCACAATTTATTATACGAACGATTTACATGCCCATGTAACCCCAGAAATTATCCCCTATGTATCCA
AGACACGTCCGGTAGGCGGCTTTGCGCCCATCTCGAAAATTGTCAAAGATGCAAAAGCGAAAGAGAAAGATGTCTTTTTC
TTTGATGCTGGCGACTATTTCACCGGACCTTTTATCAGTACGCTGACCAAAGGCGAGGCTATTATTGATATTTTAAATAC
CATGCCTTACGACGCCGTCTCTGTCGGTAACCATGAATTTGACCATGGCCATGAGAATCTGGTTAAACAGCTCAGCAAAT
TGCAATTCCCGGTATTGTTGGATAATGTTTTTTACAGCGGCACAGATACGCCATTAATTAAAGAACCGTATACCATTGTG
GAAAAAGATGGATTCAAGATCGGCGTCATCGGTATGCACGGCGTTTCCGCATTCTATGAAGCGATTGCCGCAGGCGTGCG
TGAAGGCGTTGACTGCCGCGATCCGATTCCTTATGTGAAAAAACAGCTGGAAGAGTTAAAAGGGAAAGTTGACCTGACCG
TGCTGCTCGCCCACGAAGGCGTGCCGGGTATGCAGTCCAGCGCGGGCGAGGCTGATGTCGCACGCGCACTGAAAACCGAC
GTTGATATGGCGAAATCGCTGGAAGGCTATGGACTTAACGTCCTGATTACCGGCCATGCGCATAAAGGTACGCCAGAACC
GATTAAAGTGGGCGATACTCTTGTCGTTTCCACGGATGCGTACACCATCGAATTAGGTAAACTGGTGCTTGACTGGAACC
CGGAAACCAAAAAAGTGGACAGCTACAATGGTAAGTTGATCACCATGTATGCGGATACTTATAAGCCAGATCCGGTCACG
CAGGCCAAAATTGACGAATGGGATAACAAGGTTAAGAAAATTACCGATGAAGTGGTCGCGCACTCTCCGGAAGTGCTGAC
CCGTTCTTACGGTGAATCCGCGCCAACCGGCAACTTAATCACCGATGCCCTGATGGCTACCGTTCCTGGCGCCGACGCTT
CCTTCTATAATGCTGGCGGCATCCGTACCGAATTGCCTAAAGGTAATATCACCTATGGTGATGTGCTGAGTATGTATCCG
TTCACCAACGATGTCATGAGCATGGAAATCAGCGGTAAGGACCTGAAATCCATCATGTCACACGCTGCCGATCTGAAAAA
CGGTATGCTGCACGTATCTAAAACCGTCCAGTTTAAATATGACAGTACCAAACCGCTGGGCCAGCGTATTGTTGAATTTG
ATATCAAAGGCAAACCGGTAGAAGACAATAAACTCTATACCGTCGCGCTGGATTCCTTTATCGGTAAAGGTGGTGGCGGA
TTTACCTTCACTAAAGGTAAAAATATCAAATATATAGGGATACAAACCGCACCGGCGTTGGTTAACTATATGAAGCAGGT
TAACAATATTCAACCTGACCACACCATGCGCGTGGATGATATTAGCAAATAA

Upstream 100 bases:

>100_bases
AATAATTAATAAACTTGATGAATATTACTTAATTTCCCCGGTTTGTAGTCGTGAATGACACGACTACAGGCATTGTTTTT
CTTTAATGTAGCGTAAATGG

Downstream 100 bases:

>100_bases
GCCATAATGTTATGCGGTCGATAACCGGCCGCATAACATACTTAGTTATGCAAACAGCGGCATTTTGTATACCCGAAATG
CAGAAATATCATAACGATAA

Product: secreted 5'-nucleotidase

Products: Phosphate; Uridine [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MVYMNKKFSISLLSLCIGLSSAISFSADARDITIYYTNDLHAHVTPEIIPYVSKTRPVGGFAPISKIVKDAKAKEKDVFF
FDAGDYFTGPFISTLTKGEAIIDILNTMPYDAVSVGNHEFDHGHENLVKQLSKLQFPVLLDNVFYSGTDTPLIKEPYTIV
EKDGFKIGVIGMHGVSAFYEAIAAGVREGVDCRDPIPYVKKQLEELKGKVDLTVLLAHEGVPGMQSSAGEADVARALKTD
VDMAKSLEGYGLNVLITGHAHKGTPEPIKVGDTLVVSTDAYTIELGKLVLDWNPETKKVDSYNGKLITMYADTYKPDPVT
QAKIDEWDNKVKKITDEVVAHSPEVLTRSYGESAPTGNLITDALMATVPGADASFYNAGGIRTELPKGNITYGDVLSMYP
FTNDVMSMEISGKDLKSIMSHAADLKNGMLHVSKTVQFKYDSTKPLGQRIVEFDIKGKPVEDNKLYTVALDSFIGKGGGG
FTFTKGKNIKYIGIQTAPALVNYMKQVNNIQPDHTMRVDDISK

Sequences:

>Translated_523_residues
MVYMNKKFSISLLSLCIGLSSAISFSADARDITIYYTNDLHAHVTPEIIPYVSKTRPVGGFAPISKIVKDAKAKEKDVFF
FDAGDYFTGPFISTLTKGEAIIDILNTMPYDAVSVGNHEFDHGHENLVKQLSKLQFPVLLDNVFYSGTDTPLIKEPYTIV
EKDGFKIGVIGMHGVSAFYEAIAAGVREGVDCRDPIPYVKKQLEELKGKVDLTVLLAHEGVPGMQSSAGEADVARALKTD
VDMAKSLEGYGLNVLITGHAHKGTPEPIKVGDTLVVSTDAYTIELGKLVLDWNPETKKVDSYNGKLITMYADTYKPDPVT
QAKIDEWDNKVKKITDEVVAHSPEVLTRSYGESAPTGNLITDALMATVPGADASFYNAGGIRTELPKGNITYGDVLSMYP
FTNDVMSMEISGKDLKSIMSHAADLKNGMLHVSKTVQFKYDSTKPLGQRIVEFDIKGKPVEDNKLYTVALDSFIGKGGGG
FTFTKGKNIKYIGIQTAPALVNYMKQVNNIQPDHTMRVDDISK
>Mature_523_residues
MVYMNKKFSISLLSLCIGLSSAISFSADARDITIYYTNDLHAHVTPEIIPYVSKTRPVGGFAPISKIVKDAKAKEKDVFF
FDAGDYFTGPFISTLTKGEAIIDILNTMPYDAVSVGNHEFDHGHENLVKQLSKLQFPVLLDNVFYSGTDTPLIKEPYTIV
EKDGFKIGVIGMHGVSAFYEAIAAGVREGVDCRDPIPYVKKQLEELKGKVDLTVLLAHEGVPGMQSSAGEADVARALKTD
VDMAKSLEGYGLNVLITGHAHKGTPEPIKVGDTLVVSTDAYTIELGKLVLDWNPETKKVDSYNGKLITMYADTYKPDPVT
QAKIDEWDNKVKKITDEVVAHSPEVLTRSYGESAPTGNLITDALMATVPGADASFYNAGGIRTELPKGNITYGDVLSMYP
FTNDVMSMEISGKDLKSIMSHAADLKNGMLHVSKTVQFKYDSTKPLGQRIVEFDIKGKPVEDNKLYTVALDSFIGKGGGG
FTFTKGKNIKYIGIQTAPALVNYMKQVNNIQPDHTMRVDDISK

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=561, Percent_Identity=24.2424242424242, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1786687, Length=550, Percent_Identity=24.3636363636364, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790658, Length=571, Percent_Identity=24.5183887915937, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24641187, Length=553, Percent_Identity=25.6781193490054, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI19922446, Length=556, Percent_Identity=25.5395683453237, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24654424, Length=556, Percent_Identity=25.5395683453237, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI19922444, Length=565, Percent_Identity=24.2477876106195, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28573524, Length=554, Percent_Identity=24.9097472924188, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI19921980, Length=479, Percent_Identity=24.8434237995825, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24652512, Length=471, Percent_Identity=24.6284501061571, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI161076508, Length=479, Percent_Identity=24.8434237995825, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI221329836, Length=523, Percent_Identity=23.5181644359465, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.6.1.45; 3.1.3.5 [C]

Molecular weight: Translated: 57181; Mature: 57181

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYMNKKFSISLLSLCIGLSSAISFSADARDITIYYTNDLHAHVTPEIIPYVSKTRPVGG
CEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEECCHHHHHHHHCCCCCCC
FAPISKIVKDAKAKEKDVFFFDAGDYFTGPFISTLTKGEAIIDILNTMPYDAVSVGNHEF
HHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCHHHHHHHHCCCCCEEECCCCCC
DHGHENLVKQLSKLQFPVLLDNVFYSGTDTPLIKEPYTIVEKDGFKIGVIGMHGVSAFYE
CCCHHHHHHHHHHCCCCEEEHHHEECCCCCCCCCCCCEEEECCCCEEEEEECHHHHHHHH
AIAAGVREGVDCRDPIPYVKKQLEELKGKVDLTVLLAHEGVPGMQSSAGEADVARALKTD
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
VDMAKSLEGYGLNVLITGHAHKGTPEPIKVGDTLVVSTDAYTIELGKLVLDWNPETKKVD
HHHHHCCCCCCEEEEEECCCCCCCCCCEECCCEEEEECCCEEEEEEEEEEECCCCCCEEC
SYNGKLITMYADTYKPDPVTQAKIDEWDNKVKKITDEVVAHSPEVLTRSYGESAPTGNLI
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHH
TDALMATVPGADASFYNAGGIRTELPKGNITYGDVLSMYPFTNDVMSMEISGKDLKSIMS
HHHHHHHCCCCCCCEECCCCCEEECCCCCCCHHHHHEECCCCCCEEEEEECCHHHHHHHH
HAADLKNGMLHVSKTVQFKYDSTKPLGQRIVEFDIKGKPVEDNKLYTVALDSFIGKGGGG
HHHHHHCCCEEEEEEEEEEECCCCCHHHHEEEEECCCCCCCCCCEEEEEEEHHHCCCCCC
FTFTKGKNIKYIGIQTAPALVNYMKQVNNIQPDHTMRVDDISK
EEEECCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVYMNKKFSISLLSLCIGLSSAISFSADARDITIYYTNDLHAHVTPEIIPYVSKTRPVGG
CEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEECCHHHHHHHHCCCCCCC
FAPISKIVKDAKAKEKDVFFFDAGDYFTGPFISTLTKGEAIIDILNTMPYDAVSVGNHEF
HHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCHHHHHHHHCCCCCEEECCCCCC
DHGHENLVKQLSKLQFPVLLDNVFYSGTDTPLIKEPYTIVEKDGFKIGVIGMHGVSAFYE
CCCHHHHHHHHHHCCCCEEEHHHEECCCCCCCCCCCCEEEECCCCEEEEEECHHHHHHHH
AIAAGVREGVDCRDPIPYVKKQLEELKGKVDLTVLLAHEGVPGMQSSAGEADVARALKTD
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
VDMAKSLEGYGLNVLITGHAHKGTPEPIKVGDTLVVSTDAYTIELGKLVLDWNPETKKVD
HHHHHCCCCCCEEEEEECCCCCCCCCCEECCCEEEEECCCEEEEEEEEEEECCCCCCEEC
SYNGKLITMYADTYKPDPVTQAKIDEWDNKVKKITDEVVAHSPEVLTRSYGESAPTGNLI
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHH
TDALMATVPGADASFYNAGGIRTELPKGNITYGDVLSMYPFTNDVMSMEISGKDLKSIMS
HHHHHHHCCCCCCCEECCCCCEEECCCCCCCHHHHHEECCCCCCEEEEEECCHHHHHHHH
HAADLKNGMLHVSKTVQFKYDSTKPLGQRIVEFDIKGKPVEDNKLYTVALDSFIGKGGGG
HHHHHHCCCEEEEEEEEEEECCCCCHHHHEEEEECCCCCCCCCCEEEEEEEHHHCCCCCC
FTFTKGKNIKYIGIQTAPALVNYMKQVNNIQPDHTMRVDDISK
EEEECCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UMP [C]

Specific reaction: H2O + UMP --> Phosphate + Uridine [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Inhibitor from Salmonella typhimurium cytoplasm [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]