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The map label for this gene is bcfB [H]

Identifier: 29140566

GI number: 29140566

Start: 25116

End: 25802

Strand: Direct

Name: bcfB [H]

Synonym: t0023

Alternate gene names: 29140566

Gene position: 25116-25802 (Clockwise)

Preceding gene: 29140565

Following gene: 29140567

Centisome position: 0.52

GC content: 51.24

Gene sequence:

>687_bases
ATGAAAAAAAACGTACCGATTTTCCTTCGATTATTGCTACTGCTGTCGGCGGCCGGCCTGTCGTTTGCCGTGCAGGCGGG
AGGCATTGCGCTGGGCGCGACCAGGGTGATTTATCCGCAGGGAAGTAAGCAAACCTCCTTGCCGATTATTAATTCTTCTG
CCAGCAATGTTTTTTTAATTCAGTCGTGGGTCGCCAATGCCGATGGTTCACGCTCGACGGATTTTATCATTACGCCGCCT
CTCTTTGTGATCCAGCCTAAAAAAGAAAATATATTACGTATTATGTATGTCGGGCCGTCATTACCGACCGATCGCGAAAG
CGTGTTTTATTTAAACAGTAAAGTGATACCCTCTGTCGATAAAAATAAACTGACCGGCAACTCCCTGCAAATTGCGACGC
AAAGCGTGATTAAGCTATTTATCCGCCCGAAAAATCTGGCGGAGGCGCCAGCCCATGCCCCGGCAACGCTTCGCTGCCGT
AACGAGCGCGGCCAGCTAACGATTACTAACCCTTCGCCGTACTACGTTTCGATGGTTGAACTGTACAGCGCGGGGAAAAA
ACTGCCGAATACTATGGTACCGCCGAAAGGCGCGATCACTCTCCCGGCGACGCCTGGCCAGGTATCTTTGCGCACGGTAA
ACGATTTTGGCGCAACGACGCCAGCACGCGTTTGTCCAGCTTCATGA

Upstream 100 bases:

>100_bases
TTCCTTATCGGGATTTTTTTCTCGTCTGTTGGGAGGCGAGAAAGGGGCGTTACGCGCGTCACTGCGCGTAACAAACCGAC
GGCATCAGGATATTACGCAG

Downstream 100 bases:

>100_bases
TGTTATGCGTAATCAGCTTTTCATGACGCGATATTACTCCAGCGTAGCTAAACCCGTATTAACTCCGCTGGCGTTGGCTA
TCGCACTGGCGCCTGCGCCT

Product: fimbrial chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKKNVPIFLRLLLLLSAAGLSFAVQAGGIALGATRVIYPQGSKQTSLPIINSSASNVFLIQSWVANADGSRSTDFIITPP
LFVIQPKKENILRIMYVGPSLPTDRESVFYLNSKVIPSVDKNKLTGNSLQIATQSVIKLFIRPKNLAEAPAHAPATLRCR
NERGQLTITNPSPYYVSMVELYSAGKKLPNTMVPPKGAITLPATPGQVSLRTVNDFGATTPARVCPAS

Sequences:

>Translated_228_residues
MKKNVPIFLRLLLLLSAAGLSFAVQAGGIALGATRVIYPQGSKQTSLPIINSSASNVFLIQSWVANADGSRSTDFIITPP
LFVIQPKKENILRIMYVGPSLPTDRESVFYLNSKVIPSVDKNKLTGNSLQIATQSVIKLFIRPKNLAEAPAHAPATLRCR
NERGQLTITNPSPYYVSMVELYSAGKKLPNTMVPPKGAITLPATPGQVSLRTVNDFGATTPARVCPAS
>Mature_228_residues
MKKNVPIFLRLLLLLSAAGLSFAVQAGGIALGATRVIYPQGSKQTSLPIINSSASNVFLIQSWVANADGSRSTDFIITPP
LFVIQPKKENILRIMYVGPSLPTDRESVFYLNSKVIPSVDKNKLTGNSLQIATQSVIKLFIRPKNLAEAPAHAPATLRCR
NERGQLTITNPSPYYVSMVELYSAGKKLPNTMVPPKGAITLPATPGQVSLRTVNDFGATTPARVCPAS

Specific function: Required for the biogenesis of type 1 fimbriae. Binds and interact with fimH [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786743, Length=215, Percent_Identity=50.2325581395349, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI1790771, Length=199, Percent_Identity=50.251256281407, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1787171, Length=217, Percent_Identity=47.4654377880184, Blast_Score=198, Evalue=2e-52,
Organism=Escherichia coli, GI1789532, Length=225, Percent_Identity=34.6666666666667, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1786333, Length=213, Percent_Identity=34.7417840375587, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081806, Length=231, Percent_Identity=32.9004329004329, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1786936, Length=219, Percent_Identity=31.0502283105023, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI87082203, Length=231, Percent_Identity=30.3030303030303, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788677, Length=200, Percent_Identity=34, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788428, Length=186, Percent_Identity=29.0322580645161, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 24516; Mature: 24516

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNVPIFLRLLLLLSAAGLSFAVQAGGIALGATRVIYPQGSKQTSLPIINSSASNVFLI
CCCCCHHHHHHHHHHHCCCCEEEEECCCEEECCEEEEECCCCCCCCCEEEECCCCCEEEE
QSWVANADGSRSTDFIITPPLFVIQPKKENILRIMYVGPSLPTDRESVFYLNSKVIPSVD
EEEECCCCCCCCCCEEECCCEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCC
KNKLTGNSLQIATQSVIKLFIRPKNLAEAPAHAPATLRCRNERGQLTITNPSPYYVSMVE
CCCCCCCEEEHHHHHHHHEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEHHHH
LYSAGKKLPNTMVPPKGAITLPATPGQVSLRTVNDFGATTPARVCPAS
HHHHHCCCCCCCCCCCCCEECCCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKNVPIFLRLLLLLSAAGLSFAVQAGGIALGATRVIYPQGSKQTSLPIINSSASNVFLI
CCCCCHHHHHHHHHHHCCCCEEEEECCCEEECCEEEEECCCCCCCCCEEEECCCCCEEEE
QSWVANADGSRSTDFIITPPLFVIQPKKENILRIMYVGPSLPTDRESVFYLNSKVIPSVD
EEEECCCCCCCCCCEEECCCEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCC
KNKLTGNSLQIATQSVIKLFIRPKNLAEAPAHAPATLRCRNERGQLTITNPSPYYVSMVE
CCCCCCCEEEHHHHHHHHEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEHHHH
LYSAGKKLPNTMVPPKGAITLPATPGQVSLRTVNDFGATTPARVCPAS
HHHHHCCCCCCCCCCCCCEECCCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]