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The map label for this gene is grpE

Identifier: 28572902

GI number: 28572902

Start: 858011

End: 858580

Strand: Reverse

Name: grpE

Synonym: TW761

Alternate gene names: 28572902

Gene position: 858580-858011 (Counterclockwise)

Preceding gene: 28572903

Following gene: 28572901

Centisome position: 92.73

GC content: 47.37

Gene sequence:

>570_bases
GTGACTAAGTCAAACGAGACTGAGCGCATGGAAGAGTCAGAAGAAACACACAGCTCAGATATTCGCTCGGCTAGTGAAAG
TGATCACGCTAGTGGCAGTGATCACACTGAATCAGCTGACGAGATACCCACTGCCGATGCGGAGCAAGGGGAGCTTGAAC
AGCTTGAAAAGTTGAAAGATGATTTGGCCCGTGAACGCGCGGCATTTCACAATTTCCGCATGGCACGCGCCAAACAAGCT
GAGATTGAAAGAGATCGCACTAGAAGTGAGGTCATACGTGTGATCCTACCTGTTTTAGATGATTTTGCGCGCATTGAAAA
ACATAGCACCCTGGACGATCCATTCAAGGCTGTTATAACTAAATTACGCTCTGCCATGGAAAAAATTGGTCTGACAGCTT
TTGGAAATCCGGGTGATCCGTTCAACCCTGAGCTGCATGAAGCATTATTTCAGAACCCAAGCCCAGACGTTCAAACTGAG
ACGGTGCAGGATGTTATCGAAGCTGGATATTGCTTGGGTGAAACTGTGATCAGGGCTGCAAAAGTTGTTGTACAGGTTCC
GAATGGTTAG

Upstream 100 bases:

>100_bases
GACTACGAAAGATGATCCAAAGGAACAGGTAGTTGATGCAGAGGTTGTTGATGAGGATTCGGGATCATCTAAATAGTGAA
ACTCTTAAACAGGAGCGATT

Downstream 100 bases:

>100_bases
TCAAGACTGGCTTGAGAAGGACTTTTATGCGGTTCTTGATGTTGATAGCGGGGTAAGCGGGAGTCAGATTAGCAAAGCAT
ATCGCAAACTTGCCCGTAGA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MTKSNETERMEESEETHSSDIRSASESDHASGSDHTESADEIPTADAEQGELEQLEKLKDDLARERAAFHNFRMARAKQA
EIERDRTRSEVIRVILPVLDDFARIEKHSTLDDPFKAVITKLRSAMEKIGLTAFGNPGDPFNPELHEALFQNPSPDVQTE
TVQDVIEAGYCLGETVIRAAKVVVQVPNG

Sequences:

>Translated_189_residues
MTKSNETERMEESEETHSSDIRSASESDHASGSDHTESADEIPTADAEQGELEQLEKLKDDLARERAAFHNFRMARAKQA
EIERDRTRSEVIRVILPVLDDFARIEKHSTLDDPFKAVITKLRSAMEKIGLTAFGNPGDPFNPELHEALFQNPSPDVQTE
TVQDVIEAGYCLGETVIRAAKVVVQVPNG
>Mature_188_residues
TKSNETERMEESEETHSSDIRSASESDHASGSDHTESADEIPTADAEQGELEQLEKLKDDLARERAAFHNFRMARAKQAE
IERDRTRSEVIRVILPVLDDFARIEKHSTLDDPFKAVITKLRSAMEKIGLTAFGNPGDPFNPELHEALFQNPSPDVQTET
VQDVIEAGYCLGETVIRAAKVVVQVPNG

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Saccharomyces cerevisiae, GI6324806, Length=175, Percent_Identity=27.4285714285714, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_TROW8 (Q83N75)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789682.1
- ProteinModelPortal:   Q83N75
- SMR:   Q83N75
- STRING:   Q83N75
- GeneID:   1064961
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW761
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NETERME
- ProtClustDB:   CLSK230265
- BioCyc:   TWHI218496:TW0727-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21045; Mature: 20914

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSNETERMEESEETHSSDIRSASESDHASGSDHTESADEIPTADAEQGELEQLEKLKD
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHH
DLARERAAFHNFRMARAKQAEIERDRTRSEVIRVILPVLDDFARIEKHSTLDDPFKAVIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KLRSAMEKIGLTAFGNPGDPFNPELHEALFQNPSPDVQTETVQDVIEAGYCLGETVIRAA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KVVVQVPNG
HHHHCCCCC
>Mature Secondary Structure 
TKSNETERMEESEETHSSDIRSASESDHASGSDHTESADEIPTADAEQGELEQLEKLKD
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHH
DLARERAAFHNFRMARAKQAEIERDRTRSEVIRVILPVLDDFARIEKHSTLDDPFKAVIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KLRSAMEKIGLTAFGNPGDPFNPELHEALFQNPSPDVQTETVQDVIEAGYCLGETVIRAA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KVVVQVPNG
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174