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The map label for this gene is pyrE

Identifier: 28572896

GI number: 28572896

Start: 852422

End: 853066

Strand: Reverse

Name: pyrE

Synonym: TW755

Alternate gene names: 28572896

Gene position: 853066-852422 (Counterclockwise)

Preceding gene: 28572898

Following gene: 28572890

Centisome position: 92.13

GC content: 48.68

Gene sequence:

>645_bases
GTGCAAAGGATTTTAGATTTACGCCGGATTTATGCGTTGACTGTAGATCCGCGTGCCCTTCATGGGTCCGTTTGGAAGGT
AGACTTTAAAGTGGTGAGACAGGAACTTATACGATACATAAAAGATTTAGCGCTCTTTGAAGGTGAATTTATTCTAAGTA
GTGGTCAGAAATCTTCTTATTATATCGATTTGCGCCGGGTCAGCCTTGACTGTCGCGCGGCCCCGATAATTGGCAAAGTT
ATGTTTGACCTGATATGTGACTTACAGGATGTAGATGCAATTGGCGGGCTGACCATGGGGGCCGATCCTATTGCCTGCGC
CATAATGCATTATGCAGCTTCTGTTGGGAGGTCATACAATGCGTTTGTGGTGCGAAAGCAAAAGAAAACTCATGGCCTTG
CAAGGCTGATTGAAGGGCCTGACATACGTGGCAAACGCGTTGTAATTGTTGAAGATACCTCAACAACTGGTAATAGTCCG
ATAACTGCGGCACGGCGCGCTGAGGAGACCGGGGCAACTGTTGCTGCAATCGCGGTAATGGTGGACCGCGAGACCGGGGC
AAGACAGGCTATAGAAAAGGCCGGGTACTCCTATTATGCAGCTCTCAGGGTAACAGATATCCTGGACAGAGGCACCGCAG
ATTAG

Upstream 100 bases:

>100_bases
ATTTCGTTTTCTGTCAACCAGTGCAAGACTTGACTCAATCGCGTTTTTATTGAGTTTACATTCCAAGTTGCGATTCTCAC
AATCCCAGTAAAGCAGATAA

Downstream 100 bases:

>100_bases
GCCTGCTCGAGCTGCTCGGCAAGAACATCTACTCCGTCCAGTATCTCATGAGGGCGATACGGATACCGCAGGACGTCTTC
GCGGCTGCAAATCCCGCTTA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MQRILDLRRIYALTVDPRALHGSVWKVDFKVVRQELIRYIKDLALFEGEFILSSGQKSSYYIDLRRVSLDCRAAPIIGKV
MFDLICDLQDVDAIGGLTMGADPIACAIMHYAASVGRSYNAFVVRKQKKTHGLARLIEGPDIRGKRVVIVEDTSTTGNSP
ITAARRAEETGATVAAIAVMVDRETGARQAIEKAGYSYYAALRVTDILDRGTAD

Sequences:

>Translated_214_residues
MQRILDLRRIYALTVDPRALHGSVWKVDFKVVRQELIRYIKDLALFEGEFILSSGQKSSYYIDLRRVSLDCRAAPIIGKV
MFDLICDLQDVDAIGGLTMGADPIACAIMHYAASVGRSYNAFVVRKQKKTHGLARLIEGPDIRGKRVVIVEDTSTTGNSP
ITAARRAEETGATVAAIAVMVDRETGARQAIEKAGYSYYAALRVTDILDRGTAD
>Mature_214_residues
MQRILDLRRIYALTVDPRALHGSVWKVDFKVVRQELIRYIKDLALFEGEFILSSGQKSSYYIDLRRVSLDCRAAPIIGKV
MFDLICDLQDVDAIGGLTMGADPIACAIMHYAASVGRSYNAFVVRKQKKTHGLARLIEGPDIRGKRVVIVEDTSTTGNSP
ITAARRAEETGATVAAIAVMVDRETGARQAIEKAGYSYYAALRVTDILDRGTAD

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=176, Percent_Identity=26.1363636363636, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554964, Length=181, Percent_Identity=28.1767955801105, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_TROW8 (Q83H88)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789676.1
- ProteinModelPortal:   Q83H88
- SMR:   Q83H88
- STRING:   Q83H88
- GeneID:   1064955
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW755
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   GKEADYY
- ProtClustDB:   PRK00455
- BioCyc:   TWHI218496:TW0721-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23583; Mature: 23583

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 125-125 BINDING 126-126 BINDING 129-129 BINDING 131-131 BINDING 155-155 BINDING 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRILDLRRIYALTVDPRALHGSVWKVDFKVVRQELIRYIKDLALFEGEFILSSGQKSSY
CCHHHHHHHHHEEECCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEE
YIDLRRVSLDCRAAPIIGKVMFDLICDLQDVDAIGGLTMGADPIACAIMHYAASVGRSYN
EEEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
AFVVRKQKKTHGLARLIEGPDIRGKRVVIVEDTSTTGNSPITAARRAEETGATVAAIAVM
EEEEEECHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEE
VDRETGARQAIEKAGYSYYAALRVTDILDRGTAD
EECCCCHHHHHHHCCCCEEEHHHHHHHHHCCCCC
>Mature Secondary Structure
MQRILDLRRIYALTVDPRALHGSVWKVDFKVVRQELIRYIKDLALFEGEFILSSGQKSSY
CCHHHHHHHHHEEECCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEE
YIDLRRVSLDCRAAPIIGKVMFDLICDLQDVDAIGGLTMGADPIACAIMHYAASVGRSYN
EEEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
AFVVRKQKKTHGLARLIEGPDIRGKRVVIVEDTSTTGNSPITAARRAEETGATVAAIAVM
EEEEEECHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEE
VDRETGARQAIEKAGYSYYAALRVTDILDRGTAD
EECCCCHHHHHHHCCCCEEEHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174