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The map label for this gene is purD [H]

Identifier: 28572880

GI number: 28572880

Start: 834385

End: 835644

Strand: Direct

Name: purD [H]

Synonym: TW739

Alternate gene names: 28572880

Gene position: 834385-835644 (Clockwise)

Preceding gene: 28572874

Following gene: 28572881

Centisome position: 90.11

GC content: 47.06

Gene sequence:

>1260_bases
ATGAAAATCTTAGTTATCGGCTCCGGCGCCCGAGAGCATGCAATAGCTGCAGCAATAGACTCGGAGGGGCCTCACGAAGT
ATGTGTTTCACCAGGGAATGCTGGTATAGCGCAAACAATAAACTGTGTCCAAACAACGTCCGACGCCGATCTAATCAGTA
TTGCAAAGAACTTTGATCTTGTTGTAATAGGCCCCGAAGGACCGCTTGCCGGCGGGCTTGTAGACCGGTTGAAGTCGAGT
GGGGCAACTGTATTTGGTCCTTCACAGGCCGCTGCTCGTATAGAAACTTCCAAGCACTTTGCGAAGGAAATAATGGCCAA
CGCAAATGTAAGGACTGCAAAATCTGTGCTTGTGAATAACGTAGATGATCTTTCACGGGCATTGAAAGCCAGCTCAAGGC
CATATGTTGTTAAAGCCGATGGCATTGCAGCCGGTAAGGGTGCGATTGTTACAGGTGATAGAGATGCTGCGTATCGGCAC
GGAAGTTATTGGATACGCAAGGGTCCGGTGTTGCTTGAGGAATATGTAAAAGGCCGAGAGGTCTCGCTTTTTTTTCTATG
CGATGGCAGAGTGCTTGTACCATTGAGTCCTGCCAAGGATTATAAACGGCTAGGAGAATCCGACACTGGACCCAACACTG
GAGGCATGGGATCGCATTCTCCCGTTACCGACCTTCCTGCAAATTTTGTCGAAGAAGTGCAAGAGGCAATTGCCTACCCG
GTAATAAAACAGATGGCCGCTTGCGGAATAGTATTTCGCGGGCTGCTCTATTGCGGTCTGGCACTTTGTGATGATGGTCC
AAAAGTAATTGAATTTAATGCACGTTTTGGAGATCCCGAAACTCAGGTTATTCTCCCACGACTTACCTCATCACTGTCGG
AGCTACTCTATGCAACTGCAAAAGGTGATCTACGTAACATATCGAAACCGAGTTTTAATAACAAAGCCGCGGTGTGTGTT
GTTATTGCATCCGGGGGTTACCCCGAAATACCTGCCGGGGATACACCAATTATTGGGCTAAATGGTACGGGTGATGTGCA
AGTTTTATTTGCCGGAGCAAGGAGGAATTATCCGGACGAACCCGATATACTGCACAGTCATGGCGGGAGAATAATGAACA
TCGTTTCTCTCGATAAAACATTTGCAAAAGCAAGAGAAAAGGCCTATAGGGCCGTTGACCAGATTTCTATAACAAATAAA
CAATTCAGAAGAGACATAGCCCTAGAGGAAGAGTTTATGGAAACCAATCCACTTGGGTAA

Upstream 100 bases:

>100_bases
AGGCTCCATACACGCTTCTCAAGCAGCACCGAATCCAACCTAATGATACAGCTCATAGATTTTTACCTGGGCCTTCCGCT
GAAGATAGGATTATCTTGGA

Downstream 100 bases:

>100_bases
TAAAAACAAAATCATTGGTTTGCCATCTTTGATGTAAAGGACTTTGGTAAGTGTCTTTGGTTGTTGCGCCCTGAAGGGTA
TCAGAGATATGGACAGGGTA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MKILVIGSGAREHAIAAAIDSEGPHEVCVSPGNAGIAQTINCVQTTSDADLISIAKNFDLVVIGPEGPLAGGLVDRLKSS
GATVFGPSQAAARIETSKHFAKEIMANANVRTAKSVLVNNVDDLSRALKASSRPYVVKADGIAAGKGAIVTGDRDAAYRH
GSYWIRKGPVLLEEYVKGREVSLFFLCDGRVLVPLSPAKDYKRLGESDTGPNTGGMGSHSPVTDLPANFVEEVQEAIAYP
VIKQMAACGIVFRGLLYCGLALCDDGPKVIEFNARFGDPETQVILPRLTSSLSELLYATAKGDLRNISKPSFNNKAAVCV
VIASGGYPEIPAGDTPIIGLNGTGDVQVLFAGARRNYPDEPDILHSHGGRIMNIVSLDKTFAKAREKAYRAVDQISITNK
QFRRDIALEEEFMETNPLG

Sequences:

>Translated_419_residues
MKILVIGSGAREHAIAAAIDSEGPHEVCVSPGNAGIAQTINCVQTTSDADLISIAKNFDLVVIGPEGPLAGGLVDRLKSS
GATVFGPSQAAARIETSKHFAKEIMANANVRTAKSVLVNNVDDLSRALKASSRPYVVKADGIAAGKGAIVTGDRDAAYRH
GSYWIRKGPVLLEEYVKGREVSLFFLCDGRVLVPLSPAKDYKRLGESDTGPNTGGMGSHSPVTDLPANFVEEVQEAIAYP
VIKQMAACGIVFRGLLYCGLALCDDGPKVIEFNARFGDPETQVILPRLTSSLSELLYATAKGDLRNISKPSFNNKAAVCV
VIASGGYPEIPAGDTPIIGLNGTGDVQVLFAGARRNYPDEPDILHSHGGRIMNIVSLDKTFAKAREKAYRAVDQISITNK
QFRRDIALEEEFMETNPLG
>Mature_419_residues
MKILVIGSGAREHAIAAAIDSEGPHEVCVSPGNAGIAQTINCVQTTSDADLISIAKNFDLVVIGPEGPLAGGLVDRLKSS
GATVFGPSQAAARIETSKHFAKEIMANANVRTAKSVLVNNVDDLSRALKASSRPYVVKADGIAAGKGAIVTGDRDAAYRH
GSYWIRKGPVLLEEYVKGREVSLFFLCDGRVLVPLSPAKDYKRLGESDTGPNTGGMGSHSPVTDLPANFVEEVQEAIAYP
VIKQMAACGIVFRGLLYCGLALCDDGPKVIEFNARFGDPETQVILPRLTSSLSELLYATAKGDLRNISKPSFNNKAAVCV
VIASGGYPEIPAGDTPIIGLNGTGDVQVLFAGARRNYPDEPDILHSHGGRIMNIVSLDKTFAKAREKAYRAVDQISITNK
QFRRDIALEEEFMETNPLG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=429, Percent_Identity=35.8974358974359, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4503915, Length=429, Percent_Identity=35.8974358974359, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI209869995, Length=429, Percent_Identity=35.8974358974359, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI209869993, Length=429, Percent_Identity=35.8974358974359, Blast_Score=243, Evalue=4e-64,
Organism=Escherichia coli, GI1790438, Length=433, Percent_Identity=38.1062355658199, Blast_Score=250, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17567511, Length=419, Percent_Identity=36.5155131264916, Blast_Score=216, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6321203, Length=437, Percent_Identity=33.6384439359268, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=37.2960372960373, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=37.2960372960373, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44609; Mature: 44609

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIGSGAREHAIAAAIDSEGPHEVCVSPGNAGIAQTINCVQTTSDADLISIAKNFDL
CEEEEEECCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCCHHHHHHCCCCE
VVIGPEGPLAGGLVDRLKSSGATVFGPSQAAARIETSKHFAKEIMANANVRTAKSVLVNN
EEECCCCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
VDDLSRALKASSRPYVVKADGIAAGKGAIVTGDRDAAYRHGSYWIRKGPVLLEEYVKGRE
HHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCHHCEECCCEEEECCCCHHHHHHCCCC
VSLFFLCDGRVLVPLSPAKDYKRLGESDTGPNTGGMGSHSPVTDLPANFVEEVQEAIAYP
EEEEEEECCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VIKQMAACGIVFRGLLYCGLALCDDGPKVIEFNARFGDPETQVILPRLTSSLSELLYATA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHC
KGDLRNISKPSFNNKAAVCVVIASGGYPEIPAGDTPIIGLNGTGDVQVLFAGARRNYPDE
CCCHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCC
PDILHSHGGRIMNIVSLDKTFAKAREKAYRAVDQISITNKQFRRDIALEEEFMETNPLG
CCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKILVIGSGAREHAIAAAIDSEGPHEVCVSPGNAGIAQTINCVQTTSDADLISIAKNFDL
CEEEEEECCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCCHHHHHHCCCCE
VVIGPEGPLAGGLVDRLKSSGATVFGPSQAAARIETSKHFAKEIMANANVRTAKSVLVNN
EEECCCCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
VDDLSRALKASSRPYVVKADGIAAGKGAIVTGDRDAAYRHGSYWIRKGPVLLEEYVKGRE
HHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCCHHCEECCCEEEECCCCHHHHHHCCCC
VSLFFLCDGRVLVPLSPAKDYKRLGESDTGPNTGGMGSHSPVTDLPANFVEEVQEAIAYP
EEEEEEECCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VIKQMAACGIVFRGLLYCGLALCDDGPKVIEFNARFGDPETQVILPRLTSSLSELLYATA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHC
KGDLRNISKPSFNNKAAVCVVIASGGYPEIPAGDTPIIGLNGTGDVQVLFAGARRNYPDE
CCCHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCC
PDILHSHGGRIMNIVSLDKTFAKAREKAYRAVDQISITNKQFRRDIALEEEFMETNPLG
CCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA