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The map label for this gene is purF [H]

Identifier: 28572878

GI number: 28572878

Start: 831128

End: 832588

Strand: Reverse

Name: purF [H]

Synonym: TW737

Alternate gene names: 28572878

Gene position: 832588-831128 (Counterclockwise)

Preceding gene: 28572879

Following gene: 28572877

Centisome position: 89.92

GC content: 47.78

Gene sequence:

>1461_bases
ATGTGTGGCATAGTCGGGCTAATCGGTTCAGAGCCAGCCAATCAGGCTATATACGACAGCCTCTTGCTTTTGCAGCATCG
CGGCCAGGACTCAACCGGAATAGCGACCGATGAATCAGGAATATTGCACCTCAGGAAAGCAAGAGGTTATGTTCGTGAAG
CTTTCCGCACCAGGCATATGCGATCCTTGCTTGGCCGGGTTGGCCTTGGGCATGTCAGGTACGCTACTCAGGGAAGTGTA
TCAAGCGAAGAAGAGGCACAGCCATTCTATGTAAATGCTCCGTATGGTATTGCAATGGTGCACAATGGCAATCTAACAAA
TGCAAGAAAGCTCAGTAAAGAGCTGTTCCATACAGATCGCAGACACCTTAACACGTCGAGCGACACAGAGGTTTTAATAA
ATGTTTTTGCCAACGAACTGCAACAGAGTGTCTCAGGTTTTGAATTGTCACCAGATCAAATTTTTAGTGCCGTTTCGCGC
GTGCATAAACGAGTCGAGGGGTCGTATGCGGTTGTTGCGCTAATAGCTGGCACCGGAATTTTGGCTTTCAGAGATCCGTT
CGGAATAAGACCTTTGGTATTGGGAGCCCGCGAAAGCAAATTTACTTCCAATACCGAGTGGATAATAGCGAGTGAATCAG
TTGTCCTTGAGGCGCAAGGATATAAACTGCTAAGAGATTTTGCTCCGGGTGAGGCTGTATTTATAACGCAACAGGGCGAG
ATTTTTATGACCCAATGCGCCCAAAATCCACAACTAAATCCCTGCGCCTTCGAATATGTTTATCTAGCGAGACCCGATTC
CGTTATGAATGGCGTATCGGTTTATGAGGCACGCTTGCGCCTTGGTGACACTTTATCTACAACTATCAAACAACAGATGC
CTTGCGGGAACATTGATGTTGTTATGCCCATCCCAGATTCATCGCGCCCAGCGGCGTTACAGGTCTCAAGAAATCTTGGC
ATAGAGTATCGGGAAGGGTTTTTAAAGAACCGATACGTTGGCCGAACTTTCATAATGCCAGGACAACTCCAGCGCAAGAA
AAGCGTTAGGCAGAAGCTGAATGCCATGGGTCTCGAATTCCGCGGTAAAAATGTCCTGATTGTTGACGATTCAATTGTGC
GTGGTACAACCTCAAAGGAGATTATTCACATGGCTCGCAGTGCTGGGGCAAATCAGGTATTCTTTACTTCTGCCGCGCCG
CCTGTTCGATACCAAAATGTTTTTGGAATCAACATGCCAACAAGCTCTGAGCTTGTTGCGCACAACCGGCAACTACAAGA
AATTCGTCAATACATAGACGCCGACCGCCTCATATACCAAAGTATTGAAGATATGCGACGCGCGATCATGCATGGTGGTG
AGTTCAAGCGACTTGAGGATTCGTGCTTTTCAGGCGAATACGTGACAGGGCGAGTTACCCAGGAGTACCTGGACTGGGTT
GAAAACACGCAGTCCAGCTAA

Upstream 100 bases:

>100_bases
AGTCGTAGATTCTGGCAGAAACTAGCTTTGCTGACAACAAAGAGTTGTGAATTTGGAACACTATTCGCGGGACAGTGTCC
TGCGAGGTGATGGCGCAAAA

Downstream 100 bases:

>100_bases
ATAACAGCTAGTCTGAATCCTCTGTTTTTGAGATAAGCTCCTTCTCCAGTTCCCCGAGATCCGTGCTGTCCACTCTATAC
TTCAGCTGTCTCGCAATCCT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MCGIVGLIGSEPANQAIYDSLLLLQHRGQDSTGIATDESGILHLRKARGYVREAFRTRHMRSLLGRVGLGHVRYATQGSV
SSEEEAQPFYVNAPYGIAMVHNGNLTNARKLSKELFHTDRRHLNTSSDTEVLINVFANELQQSVSGFELSPDQIFSAVSR
VHKRVEGSYAVVALIAGTGILAFRDPFGIRPLVLGARESKFTSNTEWIIASESVVLEAQGYKLLRDFAPGEAVFITQQGE
IFMTQCAQNPQLNPCAFEYVYLARPDSVMNGVSVYEARLRLGDTLSTTIKQQMPCGNIDVVMPIPDSSRPAALQVSRNLG
IEYREGFLKNRYVGRTFIMPGQLQRKKSVRQKLNAMGLEFRGKNVLIVDDSIVRGTTSKEIIHMARSAGANQVFFTSAAP
PVRYQNVFGINMPTSSELVAHNRQLQEIRQYIDADRLIYQSIEDMRRAIMHGGEFKRLEDSCFSGEYVTGRVTQEYLDWV
ENTQSS

Sequences:

>Translated_486_residues
MCGIVGLIGSEPANQAIYDSLLLLQHRGQDSTGIATDESGILHLRKARGYVREAFRTRHMRSLLGRVGLGHVRYATQGSV
SSEEEAQPFYVNAPYGIAMVHNGNLTNARKLSKELFHTDRRHLNTSSDTEVLINVFANELQQSVSGFELSPDQIFSAVSR
VHKRVEGSYAVVALIAGTGILAFRDPFGIRPLVLGARESKFTSNTEWIIASESVVLEAQGYKLLRDFAPGEAVFITQQGE
IFMTQCAQNPQLNPCAFEYVYLARPDSVMNGVSVYEARLRLGDTLSTTIKQQMPCGNIDVVMPIPDSSRPAALQVSRNLG
IEYREGFLKNRYVGRTFIMPGQLQRKKSVRQKLNAMGLEFRGKNVLIVDDSIVRGTTSKEIIHMARSAGANQVFFTSAAP
PVRYQNVFGINMPTSSELVAHNRQLQEIRQYIDADRLIYQSIEDMRRAIMHGGEFKRLEDSCFSGEYVTGRVTQEYLDWV
ENTQSS
>Mature_486_residues
MCGIVGLIGSEPANQAIYDSLLLLQHRGQDSTGIATDESGILHLRKARGYVREAFRTRHMRSLLGRVGLGHVRYATQGSV
SSEEEAQPFYVNAPYGIAMVHNGNLTNARKLSKELFHTDRRHLNTSSDTEVLINVFANELQQSVSGFELSPDQIFSAVSR
VHKRVEGSYAVVALIAGTGILAFRDPFGIRPLVLGARESKFTSNTEWIIASESVVLEAQGYKLLRDFAPGEAVFITQQGE
IFMTQCAQNPQLNPCAFEYVYLARPDSVMNGVSVYEARLRLGDTLSTTIKQQMPCGNIDVVMPIPDSSRPAALQVSRNLG
IEYREGFLKNRYVGRTFIMPGQLQRKKSVRQKLNAMGLEFRGKNVLIVDDSIVRGTTSKEIIHMARSAGANQVFFTSAAP
PVRYQNVFGINMPTSSELVAHNRQLQEIRQYIDADRLIYQSIEDMRRAIMHGGEFKRLEDSCFSGEYVTGRVTQEYLDWV
ENTQSS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=477, Percent_Identity=35.8490566037736, Blast_Score=259, Evalue=4e-69,
Organism=Escherichia coli, GI1788651, Length=488, Percent_Identity=54.7131147540984, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1790167, Length=202, Percent_Identity=30.1980198019802, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=489, Percent_Identity=32.719836400818, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6323958, Length=493, Percent_Identity=45.841784989858, Blast_Score=442, Evalue=1e-125,
Organism=Drosophila melanogaster, GI28573187, Length=506, Percent_Identity=33.399209486166, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24659598, Length=503, Percent_Identity=33.2007952286282, Blast_Score=234, Evalue=7e-62,
Organism=Drosophila melanogaster, GI24659604, Length=478, Percent_Identity=33.4728033472803, Blast_Score=232, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54257; Mature: 54257

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGLIGSEPANQAIYDSLLLLQHRGQDSTGIATDESGILHLRKARGYVREAFRTRHM
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
RSLLGRVGLGHVRYATQGSVSSEEEAQPFYVNAPYGIAMVHNGNLTNARKLSKELFHTDR
HHHHHHCCCCCEEEECCCCCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHH
RHLNTSSDTEVLINVFANELQQSVSGFELSPDQIFSAVSRVHKRVEGSYAVVALIAGTGI
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCE
LAFRDPFGIRPLVLGARESKFTSNTEWIIASESVVLEAQGYKLLRDFAPGEAVFITQQGE
EEECCCCCCCEEEECCCCCCCCCCCCEEEECCCEEEEECCHHHHHHCCCCCEEEEEECCC
IFMTQCAQNPQLNPCAFEYVYLARPDSVMNGVSVYEARLRLGDTLSTTIKQQMPCGNIDV
EEEEECCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEE
VMPIPDSSRPAALQVSRNLGIEYREGFLKNRYVGRTFIMPGQLQRKKSVRQKLNAMGLEF
EEECCCCCCCCEEEEHHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCEE
RGKNVLIVDDSIVRGTTSKEIIHMARSAGANQVFFTSAAPPVRYQNVFGINMPTSSELVA
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEECCEEEECCCCCHHHHH
HNRQLQEIRQYIDADRLIYQSIEDMRRAIMHGGEFKRLEDSCFSGEYVTGRVTQEYLDWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECHHHHHHHHHH
ENTQSS
HCCCCC
>Mature Secondary Structure
MCGIVGLIGSEPANQAIYDSLLLLQHRGQDSTGIATDESGILHLRKARGYVREAFRTRHM
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
RSLLGRVGLGHVRYATQGSVSSEEEAQPFYVNAPYGIAMVHNGNLTNARKLSKELFHTDR
HHHHHHCCCCCEEEECCCCCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHH
RHLNTSSDTEVLINVFANELQQSVSGFELSPDQIFSAVSRVHKRVEGSYAVVALIAGTGI
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCE
LAFRDPFGIRPLVLGARESKFTSNTEWIIASESVVLEAQGYKLLRDFAPGEAVFITQQGE
EEECCCCCCCEEEECCCCCCCCCCCCEEEECCCEEEEECCHHHHHHCCCCCEEEEEECCC
IFMTQCAQNPQLNPCAFEYVYLARPDSVMNGVSVYEARLRLGDTLSTTIKQQMPCGNIDV
EEEEECCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEE
VMPIPDSSRPAALQVSRNLGIEYREGFLKNRYVGRTFIMPGQLQRKKSVRQKLNAMGLEF
EEECCCCCCCCEEEEHHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCEE
RGKNVLIVDDSIVRGTTSKEIIHMARSAGANQVFFTSAAPPVRYQNVFGINMPTSSELVA
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEECCEEEECCCCCHHHHH
HNRQLQEIRQYIDADRLIYQSIEDMRRAIMHGGEFKRLEDSCFSGEYVTGRVTQEYLDWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECHHHHHHHHHH
ENTQSS
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]