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The map label for this gene is dnaX [C]

Identifier: 28572854

GI number: 28572854

Start: 806432

End: 807667

Strand: Reverse

Name: dnaX [C]

Synonym: TW713

Alternate gene names: 28572854

Gene position: 807667-806432 (Counterclockwise)

Preceding gene: 28572855

Following gene: 28572853

Centisome position: 87.23

GC content: 46.76

Gene sequence:

>1236_bases
ATGCCAAGTGGTAAGTCGCGTGCAAATACTAGCAACTCCCGCAGTGCGATGCGCGCAGTGCAGGAGCCTAGCGTAGCGTC
AGGCTCTACCGCTAATGCGCTTCCGCCCGCGGATGTATGGAATGACATCGCATGTCAGGTCCGCGCAGTGCAGGAGCTAA
AAGGAACAATTATAAATCCTAAAAGGATGATAAATAGCTGGCTGTTTTATGGACCATCACAAAGCTTTGCGCTTGCACTA
GCCTATGCATCTGCACTGCTTTCCCCGAATGCAATGAAGGGCGAACCGAATGGAGATAAAGAAACACACTCAATGGTGTC
AGCGAGAACTCATCCCGACCTGCGCATCTTGACCACTGAAAATGTTACTGTTCCAATACGAGAGATACGCGAGCTTGTAA
TGAAGGCCCAACTCGAGCCATCACTGGCGGATAAGACGGTGTTTATTGTCGAGGATTTAACCCGAGTTCCGCGCAGAAGT
CTGAATGTCCTGCTAAAACCTCTTGAAGAGCCACACGCACATGTTGTTTGGATTCTATGCGCACCGAGCATAAGGGGCGT
TACCCCAACGATTCGCTCAAGACTTAGGGCAATCTACCTTAATGAGCCTAGTATCGAAAATTTAGCGGGGTACCTTACAG
AAAGACGCGGCATAGAAAAATCCTTAGCTTGTGAAATAAGTTATGCCTCTCTCGGCAATGTAGACGATGCGCTCTATCTA
GTCGATAACGAGTCAACCAGGCTTATGCGGAAAAAGGCCCTGGATCTGGTCTTTTGTAATCGTGATTTGGCCGAGGCAGT
TTGTTCGGCTCAGACAATACTGGACCTTGTATCGAAAGAGGCTGAGAGTGTGGTTCAAAAAAGAGATTTGCAGCTTCGTG
AGGGTTTTTTGAAAAATTTTGCCTTCAAACAAGGCGATGTAATTCCAAAACAATTGGAGCATCTACAACCACGGCGGGAT
AACAAACAACAAGTTTCCAGACAGGTTAGAGATGCATTGGACGGGATTTTTCTATCCTGCCTCTCGGTGTGTAGAGACGT
GCTTGCTATAAAATCTGGCGCAACAAACTGTTTGTATAACCCGGGATCACGCATACTTGACAAACTGTCAAGCATGCCAC
CAGAGAAGATTCTACGCAACGTAAAAGCTCTAGAATATGCCCGTGATGCCCTAAAGAGCAATGTTATGCTTTTACTTCTA
CTTGAATCAAGTTTGGTGCAAATGGTAGAGATGTGA

Upstream 100 bases:

>100_bases
GCACTAGCTTTGTAACTTGCGCGGAATTTGATGTTCCTGACCATCCGGAAAAATATTCCCATGATTTACTTCGTTTGTCT
ATGAATACAATGTTTAACAA

Downstream 100 bases:

>100_bases
TTTACTAGGGTCTTATAAGACGGTTTCGGGCACTTTAAAGAAAATACGAGTCCGCTGTAGTTTACAAGGCTTGTGCCTCA
TTCCGTTGCGAACTCGTTGC

Product: DNA polymerase

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Delta Prime Subunit; DNA Polymerase III Delta Subunit; DNA-Directed DNA Polymerase; ATPase Involved In DNA Replication; DNA Polymerase III Subunit Gamma/Tau; AAA ATPase; DNA Polymerase III Delta Subunit Domain Protein; AAA ATPase Central Domain Protein; DNA Polymerase; DNA Polymerase III Delta Subunit Family Protein; DNA Polymerase III Subunit Delta Prime Subunit; DNA Polymerase III Subunit Delta Prime; DNA Polymerase III Gamma/Tau Subunits; DNA Polymerase III; DNA Polymerase III Gamma/Tau Subunit

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MPSGKSRANTSNSRSAMRAVQEPSVASGSTANALPPADVWNDIACQVRAVQELKGTIINPKRMINSWLFYGPSQSFALAL
AYASALLSPNAMKGEPNGDKETHSMVSARTHPDLRILTTENVTVPIREIRELVMKAQLEPSLADKTVFIVEDLTRVPRRS
LNVLLKPLEEPHAHVVWILCAPSIRGVTPTIRSRLRAIYLNEPSIENLAGYLTERRGIEKSLACEISYASLGNVDDALYL
VDNESTRLMRKKALDLVFCNRDLAEAVCSAQTILDLVSKEAESVVQKRDLQLREGFLKNFAFKQGDVIPKQLEHLQPRRD
NKQQVSRQVRDALDGIFLSCLSVCRDVLAIKSGATNCLYNPGSRILDKLSSMPPEKILRNVKALEYARDALKSNVMLLLL
LESSLVQMVEM

Sequences:

>Translated_411_residues
MPSGKSRANTSNSRSAMRAVQEPSVASGSTANALPPADVWNDIACQVRAVQELKGTIINPKRMINSWLFYGPSQSFALAL
AYASALLSPNAMKGEPNGDKETHSMVSARTHPDLRILTTENVTVPIREIRELVMKAQLEPSLADKTVFIVEDLTRVPRRS
LNVLLKPLEEPHAHVVWILCAPSIRGVTPTIRSRLRAIYLNEPSIENLAGYLTERRGIEKSLACEISYASLGNVDDALYL
VDNESTRLMRKKALDLVFCNRDLAEAVCSAQTILDLVSKEAESVVQKRDLQLREGFLKNFAFKQGDVIPKQLEHLQPRRD
NKQQVSRQVRDALDGIFLSCLSVCRDVLAIKSGATNCLYNPGSRILDKLSSMPPEKILRNVKALEYARDALKSNVMLLLL
LESSLVQMVEM
>Mature_410_residues
PSGKSRANTSNSRSAMRAVQEPSVASGSTANALPPADVWNDIACQVRAVQELKGTIINPKRMINSWLFYGPSQSFALALA
YASALLSPNAMKGEPNGDKETHSMVSARTHPDLRILTTENVTVPIREIRELVMKAQLEPSLADKTVFIVEDLTRVPRRSL
NVLLKPLEEPHAHVVWILCAPSIRGVTPTIRSRLRAIYLNEPSIENLAGYLTERRGIEKSLACEISYASLGNVDDALYLV
DNESTRLMRKKALDLVFCNRDLAEAVCSAQTILDLVSKEAESVVQKRDLQLREGFLKNFAFKQGDVIPKQLEHLQPRRDN
KQQVSRQVRDALDGIFLSCLSVCRDVLAIKSGATNCLYNPGSRILDKLSSMPPEKILRNVKALEYARDALKSNVMLLLLL
ESSLVQMVEM

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Tau Chain Serves As A Scaffold To Help In The Dimerizaton Of The Core Co

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 45660; Mature: 45529

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSGKSRANTSNSRSAMRAVQEPSVASGSTANALPPADVWNDIACQVRAVQELKGTIINP
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
KRMINSWLFYGPSQSFALALAYASALLSPNAMKGEPNGDKETHSMVSARTHPDLRILTTE
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
NVTVPIREIRELVMKAQLEPSLADKTVFIVEDLTRVPRRSLNVLLKPLEEPHAHVVWILC
CCEECHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEE
APSIRGVTPTIRSRLRAIYLNEPSIENLAGYLTERRGIEKSLACEISYASLGNVDDALYL
CCCCCCCCHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCHHHHEEEEHHHCCCCCCEEEE
VDNESTRLMRKKALDLVFCNRDLAEAVCSAQTILDLVSKEAESVVQKRDLQLREGFLKNF
EECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFKQGDVIPKQLEHLQPRRDNKQQVSRQVRDALDGIFLSCLSVCRDVLAIKSGATNCLYN
CCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PGSRILDKLSSMPPEKILRNVKALEYARDALKSNVMLLLLLESSLVQMVEM
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCC
>Mature Secondary Structure 
PSGKSRANTSNSRSAMRAVQEPSVASGSTANALPPADVWNDIACQVRAVQELKGTIINP
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
KRMINSWLFYGPSQSFALALAYASALLSPNAMKGEPNGDKETHSMVSARTHPDLRILTTE
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
NVTVPIREIRELVMKAQLEPSLADKTVFIVEDLTRVPRRSLNVLLKPLEEPHAHVVWILC
CCEECHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEE
APSIRGVTPTIRSRLRAIYLNEPSIENLAGYLTERRGIEKSLACEISYASLGNVDDALYL
CCCCCCCCHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCHHHHEEEEHHHCCCCCCEEEE
VDNESTRLMRKKALDLVFCNRDLAEAVCSAQTILDLVSKEAESVVQKRDLQLREGFLKNF
EECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFKQGDVIPKQLEHLQPRRDNKQQVSRQVRDALDGIFLSCLSVCRDVLAIKSGATNCLYN
CCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PGSRILDKLSSMPPEKILRNVKALEYARDALKSNVMLLLLLESSLVQMVEM
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA