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The map label for this gene is pncA [H]

Identifier: 28572852

GI number: 28572852

Start: 803796

End: 804407

Strand: Reverse

Name: pncA [H]

Synonym: TW711

Alternate gene names: 28572852

Gene position: 804407-803796 (Counterclockwise)

Preceding gene: 28572853

Following gene: 28572843

Centisome position: 86.87

GC content: 43.46

Gene sequence:

>612_bases
ATGCCCTCCCGACATGCCACGGCAGTTAATAACCGGCCAACCTCAGCTCTTCTGGTTGTTGATATACAAAAGGATTTCTG
TGAGGATGGAGCATTAGCGGTCCTTGGAGGTAATGCACTTGCCAAGCGGATATCGTGCTTTTTATCTGAGAGGCAATTAG
ACTATGCTTTAAAAATTGCCTCAAGGGACTGGCATGCACCAAACTCGAGTAATAACGGGCATTTTTCAGAAAACCCTGAT
TTCTCTAATAGCTGGCCGATTCACTGCGTTGCCAATACTCCGGGATCTGCCTATCATGATGACCTGCCTGTCGACTTGTT
TGATAAGCATATTTACAAGGGCTTTGGTAGTGCTGCATACTCTGCTTTTGAGGGTTTTGATAAAAAGGGAGTGCCATTAG
AAGAGATTCTGGCGGGTATTCAGCAAATTGATATAGCGGGCATCGCAACGGATTACTGTGTATATAAAACTGCAGTTGAC
TCCATAAAAAACGGATTTATAACCCGTGTTCTGCTTGATTTATGTGTTGAAATAAACAAAGAACGAAGCGAGGCATGTAT
AGTGCAGTTACAAGATCTCGGGGTGCACATTGTCAATAGTTATCACACATGA

Upstream 100 bases:

>100_bases
GCGCCCTAGCTGTCTAACAGTGTATGTGAGGCATAAAATAGTCGAAAAATAATTACTTCCCGCATTAAAATGAGACTCAC
TTTTGTATCATGAATTTATA

Downstream 100 bases:

>100_bases
CATATCACTACCCCGTGCCAGAAAACGCCCCGCAGCGACCGCAATAATGCAGATTGTTACACCGCGATGGCACTACCGAA
ACAACCTGACCATCCTAATG

Product: pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MPSRHATAVNNRPTSALLVVDIQKDFCEDGALAVLGGNALAKRISCFLSERQLDYALKIASRDWHAPNSSNNGHFSENPD
FSNSWPIHCVANTPGSAYHDDLPVDLFDKHIYKGFGSAAYSAFEGFDKKGVPLEEILAGIQQIDIAGIATDYCVYKTAVD
SIKNGFITRVLLDLCVEINKERSEACIVQLQDLGVHIVNSYHT

Sequences:

>Translated_203_residues
MPSRHATAVNNRPTSALLVVDIQKDFCEDGALAVLGGNALAKRISCFLSERQLDYALKIASRDWHAPNSSNNGHFSENPD
FSNSWPIHCVANTPGSAYHDDLPVDLFDKHIYKGFGSAAYSAFEGFDKKGVPLEEILAGIQQIDIAGIATDYCVYKTAVD
SIKNGFITRVLLDLCVEINKERSEACIVQLQDLGVHIVNSYHT
>Mature_202_residues
PSRHATAVNNRPTSALLVVDIQKDFCEDGALAVLGGNALAKRISCFLSERQLDYALKIASRDWHAPNSSNNGHFSENPDF
SNSWPIHCVANTPGSAYHDDLPVDLFDKHIYKGFGSAAYSAFEGFDKKGVPLEEILAGIQQIDIAGIATDYCVYKTAVDS
IKNGFITRVLLDLCVEINKERSEACIVQLQDLGVHIVNSYHT

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=189, Percent_Identity=32.2751322751323, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321401, Length=212, Percent_Identity=30.6603773584906, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22271; Mature: 22140

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRHATAVNNRPTSALLVVDIQKDFCEDGALAVLGGNALAKRISCFLSERQLDYALKIA
CCCCCCCCCCCCCCEEEEEEECCHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHEEEC
SRDWHAPNSSNNGHFSENPDFSNSWPIHCVANTPGSAYHDDLPVDLFDKHIYKGFGSAAY
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHH
SAFEGFDKKGVPLEEILAGIQQIDIAGIATDYCVYKTAVDSIKNGFITRVLLDLCVEINK
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ERSEACIVQLQDLGVHIVNSYHT
CCCHHHEEEHHHCCEEEECCCCC
>Mature Secondary Structure 
PSRHATAVNNRPTSALLVVDIQKDFCEDGALAVLGGNALAKRISCFLSERQLDYALKIA
CCCCCCCCCCCCCEEEEEEECCHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHEEEC
SRDWHAPNSSNNGHFSENPDFSNSWPIHCVANTPGSAYHDDLPVDLFDKHIYKGFGSAAY
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHH
SAFEGFDKKGVPLEEILAGIQQIDIAGIATDYCVYKTAVDSIKNGFITRVLLDLCVEINK
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ERSEACIVQLQDLGVHIVNSYHT
CCCHHHEEEHHHCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]