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The map label for this gene is nrdF [H]

Identifier: 28572842

GI number: 28572842

Start: 793275

End: 794258

Strand: Reverse

Name: nrdF [H]

Synonym: TW701

Alternate gene names: 28572842

Gene position: 794258-793275 (Counterclockwise)

Preceding gene: 28572843

Following gene: 28572841

Centisome position: 85.78

GC content: 41.16

Gene sequence:

>984_bases
ATGGGAATTTTAGGAACTGGTATACAAGATGGCTTACTTCTAAAGCCAGTGCGGTACCAATGGGCTATGGATTTGTACGA
ACAGGCTGTCGCCAACACATGGTTTCCAAACGAAATACAGCTTGGGGAAGATATAGCAGATTTTAAGAAAATGACACATG
AGGAAAAGCACGCGGTCACCTTCCTGATGAGTTACTTTAATCCGAACGAGCTTCTGGTAAATAAAGCTCTAGCATTTGGT
GTATATCCGTACATAAACGCCCCAGAATGTCATTTATACCTTGCAAAACAAATGTGGGAAGAGGCAAATCATTGCATGAG
CTTTGAATATGTCCTTGAAACGTTTCCGATTGATCGGGAAGAGGCATATTCATCGCATATTGAAGTTCCATCAATGGCTC
GCAAAGAAGAATTTGAAGTTGAATATATAAGAAGGATGACCGAAGAGACTTTGGATATAACAACAACTGAGGGTAAAAGA
GATTTTATCCGTAACCTTGTTGCGTACAACGTAGTTCTGGAGGGCATATGGTTCTACTCCGGGTTTATGGCTGCTCTGTA
CTTTAGACAAAGAAATCTTCTTCGTAATTTTGGATCACTCATAGACTGGATTGTTCGCGACGAATCTCTTCATCTTAAAT
TCGGCATAAACTTGATTCTTACCGTACTGGAGGAGAACCCAGATTTACAGACCTCCGACTTTGCTGATGAAATAAGAGGA
ATGATTATTGCTGGTGTAGAGATGGAAGAGGAGTATAACCATGACCTTCTTCCAAACGGAATCCTTGGGCTGAATAGTGG
TTACATCAATCAGTATGTCAAGTATCTAGCTGATAGACGCCTGGAGGAGCTTGGTTTTGATCCCGAATATAATGTCACAA
ACCCCGCAAAGTGGATGGCAACTGCAAATGACACGTTTGAGCTTGTAAATTTCTTTGAAGCGATCAATACTTCTTATGAA
TCCAACGCTCGCTCATTAAGCTAG

Upstream 100 bases:

>100_bases
TGTAGTAACACATTTGCATGGGTGATTTTACGCACATAAACTGCGTGGCTGAACAGAATAAAGATCTCTATACGTAAATT
AAACAGATGGAAGGTTAGGA

Downstream 100 bases:

>100_bases
AGATTAATTCGTGCGCCACGGTATCGTTCTTGAGGATTTTCGCGCGGCCGCGAAAGTAATGCACAGCAAGTTCGTTGTGC
ATACTCCGGTCATACAGTCT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MGILGTGIQDGLLLKPVRYQWAMDLYEQAVANTWFPNEIQLGEDIADFKKMTHEEKHAVTFLMSYFNPNELLVNKALAFG
VYPYINAPECHLYLAKQMWEEANHCMSFEYVLETFPIDREEAYSSHIEVPSMARKEEFEVEYIRRMTEETLDITTTEGKR
DFIRNLVAYNVVLEGIWFYSGFMAALYFRQRNLLRNFGSLIDWIVRDESLHLKFGINLILTVLEENPDLQTSDFADEIRG
MIIAGVEMEEEYNHDLLPNGILGLNSGYINQYVKYLADRRLEELGFDPEYNVTNPAKWMATANDTFELVNFFEAINTSYE
SNARSLS

Sequences:

>Translated_327_residues
MGILGTGIQDGLLLKPVRYQWAMDLYEQAVANTWFPNEIQLGEDIADFKKMTHEEKHAVTFLMSYFNPNELLVNKALAFG
VYPYINAPECHLYLAKQMWEEANHCMSFEYVLETFPIDREEAYSSHIEVPSMARKEEFEVEYIRRMTEETLDITTTEGKR
DFIRNLVAYNVVLEGIWFYSGFMAALYFRQRNLLRNFGSLIDWIVRDESLHLKFGINLILTVLEENPDLQTSDFADEIRG
MIIAGVEMEEEYNHDLLPNGILGLNSGYINQYVKYLADRRLEELGFDPEYNVTNPAKWMATANDTFELVNFFEAINTSYE
SNARSLS
>Mature_326_residues
GILGTGIQDGLLLKPVRYQWAMDLYEQAVANTWFPNEIQLGEDIADFKKMTHEEKHAVTFLMSYFNPNELLVNKALAFGV
YPYINAPECHLYLAKQMWEEANHCMSFEYVLETFPIDREEAYSSHIEVPSMARKEEFEVEYIRRMTEETLDITTTEGKRD
FIRNLVAYNVVLEGIWFYSGFMAALYFRQRNLLRNFGSLIDWIVRDESLHLKFGINLILTVLEENPDLQTSDFADEIRGM
IIAGVEMEEEYNHDLLPNGILGLNSGYINQYVKYLADRRLEELGFDPEYNVTNPAKWMATANDTFELVNFFEAINTSYES
NARSLS

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=312, Percent_Identity=26.9230769230769, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI289177074, Length=312, Percent_Identity=26.9230769230769, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4557845, Length=329, Percent_Identity=25.8358662613982, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI260064013, Length=329, Percent_Identity=25.8358662613982, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI289177076, Length=291, Percent_Identity=26.4604810996564, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1788567, Length=301, Percent_Identity=22.5913621262458, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17551968, Length=305, Percent_Identity=26.2295081967213, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI193206379, Length=286, Percent_Identity=21.3286713286713, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322434, Length=320, Percent_Identity=26.25, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321619, Length=311, Percent_Identity=23.4726688102894, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18079279, Length=308, Percent_Identity=25, Blast_Score=113, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38034; Mature: 37902

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGILGTGIQDGLLLKPVRYQWAMDLYEQAVANTWFPNEIQLGEDIADFKKMTHEEKHAVT
CCCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FLMSYFNPNELLVNKALAFGVYPYINAPECHLYLAKQMWEEANHCMSFEYVLETFPIDRE
HHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EAYSSHIEVPSMARKEEFEVEYIRRMTEETLDITTTEGKRDFIRNLVAYNVVLEGIWFYS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
GFMAALYFRQRNLLRNFGSLIDWIVRDESLHLKFGINLILTVLEENPDLQTSDFADEIRG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCHHHHHHHCC
MIIAGVEMEEEYNHDLLPNGILGLNSGYINQYVKYLADRRLEELGFDPEYNVTNPAKWMA
EEEECCCHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEE
TANDTFELVNFFEAINTSYESNARSLS
CCCHHHHHHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure 
GILGTGIQDGLLLKPVRYQWAMDLYEQAVANTWFPNEIQLGEDIADFKKMTHEEKHAVT
CCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FLMSYFNPNELLVNKALAFGVYPYINAPECHLYLAKQMWEEANHCMSFEYVLETFPIDRE
HHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EAYSSHIEVPSMARKEEFEVEYIRRMTEETLDITTTEGKRDFIRNLVAYNVVLEGIWFYS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
GFMAALYFRQRNLLRNFGSLIDWIVRDESLHLKFGINLILTVLEENPDLQTSDFADEIRG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCHHHHHHHCC
MIIAGVEMEEEYNHDLLPNGILGLNSGYINQYVKYLADRRLEELGFDPEYNVTNPAKWMA
EEEECCCHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEE
TANDTFELVNFFEAINTSYESNARSLS
CCCHHHHHHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]