Click here to switch to the map view.

The map label for this gene is sgaE [H]

Identifier: 28572810

GI number: 28572810

Start: 761023

End: 761682

Strand: Reverse

Name: sgaE [H]

Synonym: TW669

Alternate gene names: 28572810

Gene position: 761682-761023 (Counterclockwise)

Preceding gene: 28572811

Following gene: 28572809

Centisome position: 82.26

GC content: 49.39

Gene sequence:

>660_bases
TTGAGGGCTCGCGTTTGCGAGGGTAACCTGCGCCTTCCCAGAGAGGGCTTGGTTACTTGGACCTCCGGTAACTTGTCTGC
GAGAGATCCTGATACTGGGCTTGTTGTTATAAAACCTTCTGGGGTCCTGTATGAGAATATGACTCCGGAGGATATGGTTG
TTGTTTCCCTCGACGGTGAAATTGTTGAAGGTTCTATGCCGCCTTCGTCCGATACCGCGTCCCATTTGGGCGTGTACCGC
AAGCGGGATGATGTTGGCAGTATTGTGCACACTCATTCTAGGTATGCTACTGCATTTGCTGCGGTTGGAAAAGAGATTCC
ATGCTGTATAACTGCAGTCGCTGATGAATTTGGTGGTCCAATTCCTTGTGGGGATTATGCGGTCATTGGCGGCGAAGAGA
TTGGCGAAGAGATTGTCAGAAAGATTGGTCACTCTCCGGCTATACTCATGAAACAGCATGGAGTTTTTACCGTTGGCGCG
ACCATACACGATGCGCTCAAAGCTGCGGTTATGGTCGAGGATGTCGCAAGAACAATCTTTGCTGCGATGCAGATAGGCGA
TATTGAGCCTTTGCCTGAGGCCGAAATCAGGCGAAATTATGAGAGATATAAAAATAGATACGGGACTATGTCCGCGAGTG
ATGGGGTAAAAAGTGCCTAG

Upstream 100 bases:

>100_bases
GTCCTGACTCTGAGCGCATTGCCTCGCGCGCTCGGGAGTTCATAACTGCACGGCTCGGTGAGTCTGGTATCGCTGTTGTC
TCTAAGGCATGTTAGAGGAT

Downstream 100 bases:

>100_bases
ATACGATGTTGTTTCTTTGGGCGAGGGTCAGATACGTCTCACCGTTCCACGTGGAGAGAATCTTCTTACGGCAAAGCAGC
TTTACTTGACTGCAGCCGGT

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MRARVCEGNLRLPREGLVTWTSGNLSARDPDTGLVVIKPSGVLYENMTPEDMVVVSLDGEIVEGSMPPSSDTASHLGVYR
KRDDVGSIVHTHSRYATAFAAVGKEIPCCITAVADEFGGPIPCGDYAVIGGEEIGEEIVRKIGHSPAILMKQHGVFTVGA
TIHDALKAAVMVEDVARTIFAAMQIGDIEPLPEAEIRRNYERYKNRYGTMSASDGVKSA

Sequences:

>Translated_219_residues
MRARVCEGNLRLPREGLVTWTSGNLSARDPDTGLVVIKPSGVLYENMTPEDMVVVSLDGEIVEGSMPPSSDTASHLGVYR
KRDDVGSIVHTHSRYATAFAAVGKEIPCCITAVADEFGGPIPCGDYAVIGGEEIGEEIVRKIGHSPAILMKQHGVFTVGA
TIHDALKAAVMVEDVARTIFAAMQIGDIEPLPEAEIRRNYERYKNRYGTMSASDGVKSA
>Mature_219_residues
MRARVCEGNLRLPREGLVTWTSGNLSARDPDTGLVVIKPSGVLYENMTPEDMVVVSLDGEIVEGSMPPSSDTASHLGVYR
KRDDVGSIVHTHSRYATAFAAVGKEIPCCITAVADEFGGPIPCGDYAVIGGEEIGEEIVRKIGHSPAILMKQHGVFTVGA
TIHDALKAAVMVEDVARTIFAAMQIGDIEPLPEAEIRRNYERYKNRYGTMSASDGVKSA

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790642, Length=196, Percent_Identity=47.9591836734694, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1790008, Length=217, Percent_Identity=41.4746543778802, Blast_Score=146, Evalue=9e-37,
Organism=Escherichia coli, GI1786247, Length=217, Percent_Identity=40.0921658986175, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1789164, Length=206, Percent_Identity=31.5533980582524, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1789094, Length=171, Percent_Identity=26.9005847953216, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 23532; Mature: 23532

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARVCEGNLRLPREGLVTWTSGNLSARDPDTGLVVIKPSGVLYENMTPEDMVVVSLDGE
CCCEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCEEECCCCCCCEEEEEECCE
IVEGSMPPSSDTASHLGVYRKRDDVGSIVHTHSRYATAFAAVGKEIPCCITAVADEFGGP
EEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
IPCGDYAVIGGEEIGEEIVRKIGHSPAILMKQHGVFTVGATIHDALKAAVMVEDVARTIF
CCCCCEEEECHHHHHHHHHHHHCCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHH
AAMQIGDIEPLPEAEIRRNYERYKNRYGTMSASDGVKSA
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRARVCEGNLRLPREGLVTWTSGNLSARDPDTGLVVIKPSGVLYENMTPEDMVVVSLDGE
CCCEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCEEECCCCCCCEEEEEECCE
IVEGSMPPSSDTASHLGVYRKRDDVGSIVHTHSRYATAFAAVGKEIPCCITAVADEFGGP
EEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
IPCGDYAVIGGEEIGEEIVRKIGHSPAILMKQHGVFTVGATIHDALKAAVMVEDVARTIF
CCCCCEEEECHHHHHHHHHHHHCCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHH
AAMQIGDIEPLPEAEIRRNYERYKNRYGTMSASDGVKSA
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA