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The map label for this gene is folD [H]

Identifier: 28572797

GI number: 28572797

Start: 747349

End: 748224

Strand: Reverse

Name: folD [H]

Synonym: TW653

Alternate gene names: 28572797

Gene position: 748224-747349 (Counterclockwise)

Preceding gene: 28572798

Following gene: 28572796

Centisome position: 80.81

GC content: 44.98

Gene sequence:

>876_bases
GTGCTTGATGGACTCGCAGTGTCGGCCGGTATATTTGAGCGGCTACACAGAGCGTGTTGCGAGCTGGAGAATAATGGGGT
TTGTCCAAAACTTGCAACTGTCTTGGTTGGCAATAACCCGGCATCACGCTCATATGTGCTCAGTAAGCATAGGGATTGTA
ACAATATAGGAATCCAATCAACACTCATTGAGCTTCCTGCAAATACGTCTGAAAAGGAACTTATATCAAAAGTAGAAATT
TTAAACGCATCAAGCGATGTATCAGGGATAATAGTCCAACTGCCCCTGCCGGAGAGTATAGATCAAAATAAAGTCATAGA
AGCAATTAATCCCGATAAGGATGCTGACGGGTTACATCCACTCAATTTGGGGTATCTTGTTACAGGCAAACAGGGTATTG
TGCCCTGCACTCCTGCGGGTATTGTAAAACTACTCGAAGCTCACAGAATAACCCTTGAGGGAAAAACAATTGCTGTTATA
GGACGGGGAACAACCGTCGGTAGGCCACTTGGCATTTTACTTTCAGGAAAAGGGGTAAATGCAACAGTTATAAATATCCA
TTCCCGCAGCAGGAATATTTCGGGTTTATCAAGGATTGCCGATGTGGTGGTCGCTTGTGCAGGGGTCAAGCATATTGTCG
AACCATCATGGATAAAACCCGGTGCGGTGGTTGTGGATGTTGGAATAAATCAGTCAGGAGTGAGCAGCTCGGGGAAAATG
ATACTAACCGGCGACGTCCATCCGCTCACAAAGAATATTGCAAGCTATATCTCACCCGTAGTAGGTGGGGTTGGGCCAAT
GACCCGTGCAATGCTTATGTCAAATGTTATAAACCTCTCAGAGAGAGATTTCAGAAAAAGGTTGTATGCTTCATAG

Upstream 100 bases:

>100_bases
CTTGCATTATCCCGTAACATTATCTCTTTATCTGTAGGCCTTGCTGTTCAGCCAACTTGACAATCCCAACACGATACACT
GAAATACAGTGGTAGCAAAA

Downstream 100 bases:

>100_bases
GTGAGTAAGATAAAGCTTGTAACCGCGATACCCGGCCCTGAATCAGAGAGGCTACACAGGATAAGGCAGGCCACTGTTGC
AAGGGGTGTGTCGTCCACTT

Product: methylenetetrahydrofolate dehydrogenase; methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLDGLAVSAGIFERLHRACCELENNGVCPKLATVLVGNNPASRSYVLSKHRDCNNIGIQSTLIELPANTSEKELISKVEI
LNASSDVSGIIVQLPLPESIDQNKVIEAINPDKDADGLHPLNLGYLVTGKQGIVPCTPAGIVKLLEAHRITLEGKTIAVI
GRGTTVGRPLGILLSGKGVNATVINIHSRSRNISGLSRIADVVVACAGVKHIVEPSWIKPGAVVVDVGINQSGVSSSGKM
ILTGDVHPLTKNIASYISPVVGGVGPMTRAMLMSNVINLSERDFRKRLYAS

Sequences:

>Translated_291_residues
MLDGLAVSAGIFERLHRACCELENNGVCPKLATVLVGNNPASRSYVLSKHRDCNNIGIQSTLIELPANTSEKELISKVEI
LNASSDVSGIIVQLPLPESIDQNKVIEAINPDKDADGLHPLNLGYLVTGKQGIVPCTPAGIVKLLEAHRITLEGKTIAVI
GRGTTVGRPLGILLSGKGVNATVINIHSRSRNISGLSRIADVVVACAGVKHIVEPSWIKPGAVVVDVGINQSGVSSSGKM
ILTGDVHPLTKNIASYISPVVGGVGPMTRAMLMSNVINLSERDFRKRLYAS
>Mature_291_residues
MLDGLAVSAGIFERLHRACCELENNGVCPKLATVLVGNNPASRSYVLSKHRDCNNIGIQSTLIELPANTSEKELISKVEI
LNASSDVSGIIVQLPLPESIDQNKVIEAINPDKDADGLHPLNLGYLVTGKQGIVPCTPAGIVKLLEAHRITLEGKTIAVI
GRGTTVGRPLGILLSGKGVNATVINIHSRSRNISGLSRIADVVVACAGVKHIVEPSWIKPGAVVVDVGINQSGVSSSGKM
ILTGDVHPLTKNIASYISPVVGGVGPMTRAMLMSNVINLSERDFRKRLYAS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=274, Percent_Identity=39.051094890511, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=37.4149659863946, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI222418558, Length=258, Percent_Identity=36.8217054263566, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI36796743, Length=172, Percent_Identity=31.3953488372093, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1786741, Length=251, Percent_Identity=45.0199203187251, Blast_Score=194, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17568735, Length=260, Percent_Identity=38.4615384615385, Blast_Score=158, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6319558, Length=290, Percent_Identity=39.3103448275862, Blast_Score=195, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6321643, Length=274, Percent_Identity=37.956204379562, Blast_Score=167, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322933, Length=298, Percent_Identity=25.503355704698, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=36.3636363636364, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=36.3636363636364, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI62472483, Length=298, Percent_Identity=39.261744966443, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI45551871, Length=298, Percent_Identity=39.261744966443, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24645718, Length=298, Percent_Identity=39.261744966443, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI17137370, Length=298, Percent_Identity=39.261744966443, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30712; Mature: 30712

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00766 THF_DHG_CYH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDGLAVSAGIFERLHRACCELENNGVCPKLATVLVGNNPASRSYVLSKHRDCNNIGIQS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCCCCHHHCCCCCCCCCCCC
TLIELPANTSEKELISKVEILNASSDVSGIIVQLPLPESIDQNKVIEAINPDKDADGLHP
EEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCC
LNLGYLVTGKQGIVPCTPAGIVKLLEAHRITLEGKTIAVIGRGTTVGRPLGILLSGKGVN
CCCCEEEECCCCCCCCCHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCEEEEEECCCCC
ATVINIHSRSRNISGLSRIADVVVACAGVKHIVEPSWIKPGAVVVDVGINQSGVSSSGKM
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCE
ILTGDVHPLTKNIASYISPVVGGVGPMTRAMLMSNVINLSERDFRKRLYAS
EEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MLDGLAVSAGIFERLHRACCELENNGVCPKLATVLVGNNPASRSYVLSKHRDCNNIGIQS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCCCCHHHCCCCCCCCCCCC
TLIELPANTSEKELISKVEILNASSDVSGIIVQLPLPESIDQNKVIEAINPDKDADGLHP
EEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCC
LNLGYLVTGKQGIVPCTPAGIVKLLEAHRITLEGKTIAVIGRGTTVGRPLGILLSGKGVN
CCCCEEEECCCCCCCCCHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCEEEEEECCCCC
ATVINIHSRSRNISGLSRIADVVVACAGVKHIVEPSWIKPGAVVVDVGINQSGVSSSGKM
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCE
ILTGDVHPLTKNIASYISPVVGGVGPMTRAMLMSNVINLSERDFRKRLYAS
EEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174 [H]