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The map label for this gene is rph [H]

Identifier: 28572785

GI number: 28572785

Start: 727191

End: 727961

Strand: Reverse

Name: rph [H]

Synonym: TW640

Alternate gene names: 28572785

Gene position: 727961-727191 (Counterclockwise)

Preceding gene: 28572788

Following gene: 28572784

Centisome position: 78.62

GC content: 46.95

Gene sequence:

>771_bases
GTGTCTGTCACCTCGCTGGCGGTGTATATGCTGTTTTGTTGTATTATTTGCTCTGTGTTGAGGAAGAATTCTAGGGCACA
TGACGAAATTCGACCCGTAAAAATTATCCGCGGGTGGAATATTTATGCAGAGGGATCAGCCCTAATTGCTTTTGGCAATA
CCAGGGTTCTTTGTAATGCAACCTTTCAGCGCGGAGTTCCGCCGTTTTTACGCGGACAGAGATCTGGATGGATAACGGCA
GAATATGCTATGCTCCCGCGATCTGGTACCGAGCGATCTGACCGTGAATCTGTTAAGGGGAAAATTAGCGGTCGCAGTCA
TGAAATCAGTCGCCTCATAGGCCGAAGTATGCGTGCAATTTTGGATCGGTATGCTCTGGAGGAGAATACCATTATCCTAG
ATTGTGATGTCCTACAGGCCGACGGCGGGACGCGCACCGCCGCTATAACCGGTTCGTATATTGCGCTTTACGATGCCCTT
GTCTGGGCTAAAAATCAAAAGATACTTTCAAAGCATCCTCTGACTGATTCTGTATCCGCTGTGTCTGTTGGGCTAGTCGG
CGACCAGATTTTTCTAGACCTGGATTATTCGGAGGACTCAAATGCACAGGCTGACATTAATCTTGTTTTTACTGGAAGCG
GTAAATTAGTAGAGATTCAGGGTACTGCAGAAAAATCACCCTTTTCGTATGGACAGTTTGAGCAGATGATGGAACTTGCC
AAAACAGGTTGCCAAGCCCTAAAGGAGATACAGGCCGCTTCATTGGATTAA

Upstream 100 bases:

>100_bases
GCAGCTATCCCTGATATTCGTGCTGCGTTGACCATCGACAACTCGTATTGGTCTGTTAGGAGACCTGCACCGCCCACATG
ATAAGTGTATATGGAGTTAC

Downstream 100 bases:

>100_bases
AAATAATCTGGATTGACTCTATTAAATGGAAATTGTATTCGTTTCAGAAAATGAGAATAAGATTACCGAGGCTCGCGAAA
TCCTGTTGCCACTCGGATTT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSVTSLAVYMLFCCIICSVLRKNSRAHDEIRPVKIIRGWNIYAEGSALIAFGNTRVLCNATFQRGVPPFLRGQRSGWITA
EYAMLPRSGTERSDRESVKGKISGRSHEISRLIGRSMRAILDRYALEENTIILDCDVLQADGGTRTAAITGSYIALYDAL
VWAKNQKILSKHPLTDSVSAVSVGLVGDQIFLDLDYSEDSNAQADINLVFTGSGKLVEIQGTAEKSPFSYGQFEQMMELA
KTGCQALKEIQAASLD

Sequences:

>Translated_256_residues
MSVTSLAVYMLFCCIICSVLRKNSRAHDEIRPVKIIRGWNIYAEGSALIAFGNTRVLCNATFQRGVPPFLRGQRSGWITA
EYAMLPRSGTERSDRESVKGKISGRSHEISRLIGRSMRAILDRYALEENTIILDCDVLQADGGTRTAAITGSYIALYDAL
VWAKNQKILSKHPLTDSVSAVSVGLVGDQIFLDLDYSEDSNAQADINLVFTGSGKLVEIQGTAEKSPFSYGQFEQMMELA
KTGCQALKEIQAASLD
>Mature_255_residues
SVTSLAVYMLFCCIICSVLRKNSRAHDEIRPVKIIRGWNIYAEGSALIAFGNTRVLCNATFQRGVPPFLRGQRSGWITAE
YAMLPRSGTERSDRESVKGKISGRSHEISRLIGRSMRAILDRYALEENTIILDCDVLQADGGTRTAAITGSYIALYDALV
WAKNQKILSKHPLTDSVSAVSVGLVGDQIFLDLDYSEDSNAQADINLVFTGSGKLVEIQGTAEKSPFSYGQFEQMMELAK
TGCQALKEIQAASLD

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=52.60663507109, Blast_Score=234, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI71981632, Length=237, Percent_Identity=26.5822784810127, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 28096; Mature: 27965

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTSLAVYMLFCCIICSVLRKNSRAHDEIRPVKIIRGWNIYAEGSALIAFGNTRVLCNA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEECCCEEEEECCCEEEEEE
TFQRGVPPFLRGQRSGWITAEYAMLPRSGTERSDRESVKGKISGRSHEISRLIGRSMRAI
HHHHCCCHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHCEECCCCHHHHHHHHHHHHHHH
LDRYALEENTIILDCDVLQADGGTRTAAITGSYIALYDALVWAKNQKILSKHPLTDSVSA
HHHHHCCCCCEEEEEEEEECCCCCEEEEEECCHHHHHHHHHHHCCCHHHHCCCCCCCHHH
VSVGLVGDQIFLDLDYSEDSNAQADINLVFTGSGKLVEIQGTAEKSPFSYGQFEQMMELA
EEEEEECCEEEEEEECCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHH
KTGCQALKEIQAASLD
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVTSLAVYMLFCCIICSVLRKNSRAHDEIRPVKIIRGWNIYAEGSALIAFGNTRVLCNA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEECCCEEEEECCCEEEEEE
TFQRGVPPFLRGQRSGWITAEYAMLPRSGTERSDRESVKGKISGRSHEISRLIGRSMRAI
HHHHCCCHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHCEECCCCHHHHHHHHHHHHHHH
LDRYALEENTIILDCDVLQADGGTRTAAITGSYIALYDALVWAKNQKILSKHPLTDSVSA
HHHHHCCCCCEEEEEEEEECCCCCEEEEEECCHHHHHHHHHHHCCCHHHHCCCCCCCHHH
VSVGLVGDQIFLDLDYSEDSNAQADINLVFTGSGKLVEIQGTAEKSPFSYGQFEQMMELA
EEEEEECCEEEEEEECCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHH
KTGCQALKEIQAASLD
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174 [H]