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The map label for this gene is nusA [H]

Identifier: 28572761

GI number: 28572761

Start: 700744

End: 701703

Strand: Reverse

Name: nusA [H]

Synonym: TW615

Alternate gene names: 28572761

Gene position: 701703-700744 (Counterclockwise)

Preceding gene: 28572762

Following gene: 28572760

Centisome position: 75.78

GC content: 48.96

Gene sequence:

>960_bases
GTGCATATGAAGATTGATTTATCAGTTCTGCGTGCGGTTGAGCGCGAGCAGCGTGTCGCTTTTGATGACCTAGTCCGCAT
CGCTGAGCAGGCGGTTGTTATGGCTTACCTTAGACATACTGGCCTGGAGGATGCGGATCTTGTGAAGGCAAAGCTCAACC
GCAAGACCGGCGAGATCGATCTGCGCGTCAAACACGATGGCGATTTTATCCCGTACAAATCAGATGCTTTTAGCCGGATG
GCTGCCACTGCAGCCAAGCGTGTTATAACGAGCAAAATGCGCACCCTTAGTGACGATTCGACGTTTGCCCGATACAAAAC
AAAGATCGGCACCGTCCTGTCTGGAGTTATTTCGCAGAGTACTTCTCAAAACTTTAGGATGGTTTCTCTCGATCGTCGGA
CAGAAGCAATTTTACGCCCTGAAGAGCAGATACCTGGTGAGGAATACCCGCACGGTAAGATGGTCAAGGTTTATGTTACT
GATGTTACACTGGGCCCCCGGGGGTCTAATGTTTATGTTTCACGAACCCATCCTGGCCTGATTAACGGTCTGTTTTTACA
AGAAGTGCCAGAGATCGCCTCCGGCACTGTGGAGATTGTTGCTGTTTCGCGAGAGGCAGGTCATCGTACAAAAGTCTCTG
TCAGGAGTAATAGCAAGTCAACAAGTGCGGTGGGTGCATGTGTTGGAGAGTATGGCTGCCGTGTGCGTGCTGTTACCTCG
GAGATCTGTAATGAAAAGATAGATATAATAAGTTACTCTGATGATCTTGGTCAGTACGTGGCAAATGCTATTTCTCCGGC
GACTGCTGCTGATGTTTTTGTCTTAGATTCTAGACAGAAGGTTGTTCGTGTGTATGTTTCGAAGGAGGAGTACTCCCTTG
CAATTGGCAAGGAGGGTCAGAATGCCAGGCTTGCTGCTCGTCTGACGGGTGCAAAGATTGATATCAGGATTGACGAGTAG

Upstream 100 bases:

>100_bases
TACGTACACTGTAGATCATGGTGGCACGCCTGCGCCCGTAAATGGGCACCTGAAGCCTCTATGTGTGGATGTATGAATAT
GCTGGCCTTGATGCACTGAG

Downstream 100 bases:

>100_bases
GTCTTGGCGTATGTGTGCTGTGTATCTATCAGATGTATTTGGTGTATTTTATGCAAATAACATATATCTTGGTAACGTAG
CGTGAATACAATGACGAGTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MHMKIDLSVLRAVEREQRVAFDDLVRIAEQAVVMAYLRHTGLEDADLVKAKLNRKTGEIDLRVKHDGDFIPYKSDAFSRM
AATAAKRVITSKMRTLSDDSTFARYKTKIGTVLSGVISQSTSQNFRMVSLDRRTEAILRPEEQIPGEEYPHGKMVKVYVT
DVTLGPRGSNVYVSRTHPGLINGLFLQEVPEIASGTVEIVAVSREAGHRTKVSVRSNSKSTSAVGACVGEYGCRVRAVTS
EICNEKIDIISYSDDLGQYVANAISPATAADVFVLDSRQKVVRVYVSKEEYSLAIGKEGQNARLAARLTGAKIDIRIDE

Sequences:

>Translated_319_residues
MHMKIDLSVLRAVEREQRVAFDDLVRIAEQAVVMAYLRHTGLEDADLVKAKLNRKTGEIDLRVKHDGDFIPYKSDAFSRM
AATAAKRVITSKMRTLSDDSTFARYKTKIGTVLSGVISQSTSQNFRMVSLDRRTEAILRPEEQIPGEEYPHGKMVKVYVT
DVTLGPRGSNVYVSRTHPGLINGLFLQEVPEIASGTVEIVAVSREAGHRTKVSVRSNSKSTSAVGACVGEYGCRVRAVTS
EICNEKIDIISYSDDLGQYVANAISPATAADVFVLDSRQKVVRVYVSKEEYSLAIGKEGQNARLAARLTGAKIDIRIDE
>Mature_319_residues
MHMKIDLSVLRAVEREQRVAFDDLVRIAEQAVVMAYLRHTGLEDADLVKAKLNRKTGEIDLRVKHDGDFIPYKSDAFSRM
AATAAKRVITSKMRTLSDDSTFARYKTKIGTVLSGVISQSTSQNFRMVSLDRRTEAILRPEEQIPGEEYPHGKMVKVYVT
DVTLGPRGSNVYVSRTHPGLINGLFLQEVPEIASGTVEIVAVSREAGHRTKVSVRSNSKSTSAVGACVGEYGCRVRAVTS
EICNEKIDIISYSDDLGQYVANAISPATAADVFVLDSRQKVVRVYVSKEEYSLAIGKEGQNARLAARLTGAKIDIRIDE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=251, Percent_Identity=33.4661354581673, Blast_Score=156, Evalue=2e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N [H]

EC number: NA

Molecular weight: Translated: 35171; Mature: 35171

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMKIDLSVLRAVEREQRVAFDDLVRIAEQAVVMAYLRHTGLEDADLVKAKLNRKTGEID
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEE
LRVKHDGDFIPYKSDAFSRMAATAAKRVITSKMRTLSDDSTFARYKTKIGTVLSGVISQS
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
TSQNFRMVSLDRRTEAILRPEEQIPGEEYPHGKMVKVYVTDVTLGPRGSNVYVSRTHPGL
CCCCEEEEEECCHHHHHCCCHHHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCCH
INGLFLQEVPEIASGTVEIVAVSREAGHRTKVSVRSNSKSTSAVGACVGEYGCRVRAVTS
HHHHHHHHHHHHHCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEHHH
EICNEKIDIISYSDDLGQYVANAISPATAADVFVLDSRQKVVRVYVSKEEYSLAIGKEGQ
HHCCCCEEEEEECHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCEEEEECCCCC
NARLAARLTGAKIDIRIDE
CCEEEEEEECCEEEEEECC
>Mature Secondary Structure
MHMKIDLSVLRAVEREQRVAFDDLVRIAEQAVVMAYLRHTGLEDADLVKAKLNRKTGEID
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEE
LRVKHDGDFIPYKSDAFSRMAATAAKRVITSKMRTLSDDSTFARYKTKIGTVLSGVISQS
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
TSQNFRMVSLDRRTEAILRPEEQIPGEEYPHGKMVKVYVTDVTLGPRGSNVYVSRTHPGL
CCCCEEEEEECCHHHHHCCCHHHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCCH
INGLFLQEVPEIASGTVEIVAVSREAGHRTKVSVRSNSKSTSAVGACVGEYGCRVRAVTS
HHHHHHHHHHHHHCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEHHH
EICNEKIDIISYSDDLGQYVANAISPATAADVFVLDSRQKVVRVYVSKEEYSLAIGKEGQ
HHCCCCEEEEEECHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCEEEEECCCCC
NARLAARLTGAKIDIRIDE
CCEEEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]