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The map label for this gene is tyrS

Identifier: 28572750

GI number: 28572750

Start: 683275

End: 684690

Strand: Reverse

Name: tyrS

Synonym: TW603

Alternate gene names: 28572750

Gene position: 684690-683275 (Counterclockwise)

Preceding gene: 28572751

Following gene: 28572749

Centisome position: 73.95

GC content: 47.95

Gene sequence:

>1416_bases
ATGCCAAATGCCCCGGAAGTCAATAGTGTCTGGGATGAGCTATTGTGGCGGGGCCTTGTGTGTGTATCAACAGACCAAGG
GGCATTAAAAGAACTGCTTGACGGTCCACCCATCAGGTTTTATTGTGGCTTTGACCCCACGGCGCCAAGTTTGCATGTCG
GTAACCTGGCGCAGATTCTCATTATGCGTCGACTACAGCTCGCGGGTCACAGGCCTATTGCTCTTGTTGGCGGCTCTACG
GGACTGATAGGAGATCCAAGGCCAGGAGGTGAAAGGCAACTTCATTCGCACGAACAGGTACAAGAGTGGGTGCGTGCTCT
ACAACAACAATTATCTAGGTTTCTTGACTTTAAGGGCGCGGCTGCTGCGGTGCTCGTAAATAATTTAGACTGGACCAAGT
CCCTGACTGCACTTGATTTTCTACGTGAACTTGGAAAACATTTTCGTGTGAATGCAATGCTAAAAAAGGACGCAGTTGCC
GCGAGACTTTCCTCCACTGAGGGTATAAGTTACACAGAGTTTAGTTACCAGATACTCCAATCTCTCGACTTCAGGCAGCT
CTATCTAGACTATAAATGTGTTTTGCAAATAGGTGGTAGCGATCAGTGGGGGAATATAACATCCGGCGTGGATCTTATTC
GCAAAACCGAAGGTGCGGGTGTGCATGCCTTTGGTTCGCCCCTTCTAACAGCCTCTGATGGGTCTAAATTCGGAAAAACT
GAAGGCAATGCTATTTGGCTTGACGCCGATCTAACCAGCCCGTGGTCCTTTTACCAATTCTTTCTGAATTCCGATGATGC
GGATATACCAAGACTTCTTAGGGTTTTCACATTTTTTACCCGTTCTGAGATAGAAGACCTGAACAGGAGCGTTCGTGAGA
ATGCATCTGCCAGACTTGCTCACAGGCATCTTGCATACTCTGTTACACGGCTGGTTCATGGCGCTGATGTTGCTGATCAG
GTATTGTTGGCGTGTTCCGTGCTGTTTGGAGATGGCGGCGCGCTTGGCAAAACGCAAACAGGACTGCCTCGTATAGACTC
GGATACTTTAAATGATTCATCTGGAGTAACAACTATAAAAGCGCAAGACAATACATATACACCCTACTCTCCGGAAGGTG
TGCGCTTTGCAGGTACATTTCCCGTTGATCCTCGGTTTCTGCGATCGATTTTATTTGAACTGCCGAATGCGACCGTTAGT
GCCCATGACCTCATTACACATATTCTGCAGAAAGTTGGTTTGTGCAGAAGTCTCTCTGAGGCGCGGCGGCTTATCAGCCA
AGGGGGAATTTATGTTAATAACATAAAGGTAACTTGCCCCGACGCCCTTCTTGATTCCTTTCTGGATAATTCAATGCCGA
TCAGAGCCGCCATTCTGCGCAAGGGCAAGAAACACCTTGCTGCTGTTTTTTACTGA

Upstream 100 bases:

>100_bases
CCTAATTATTGCATATATACAGCAACTATTGGCACATATGCTTTGGCATTACGGTTGTATCTAGTAACGCTTACGAATCC
GCCCAGGATATACTAACTTC

Downstream 100 bases:

>100_bases
GTATGTATGGTGTATTCATACTGCGCCTGAAGAACAATTTATCCTGAAGAACAATTTAAGAACAATTGGTTACTCTATAC
CCTCTTTCACCCATCTAACC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MPNAPEVNSVWDELLWRGLVCVSTDQGALKELLDGPPIRFYCGFDPTAPSLHVGNLAQILIMRRLQLAGHRPIALVGGST
GLIGDPRPGGERQLHSHEQVQEWVRALQQQLSRFLDFKGAAAAVLVNNLDWTKSLTALDFLRELGKHFRVNAMLKKDAVA
ARLSSTEGISYTEFSYQILQSLDFRQLYLDYKCVLQIGGSDQWGNITSGVDLIRKTEGAGVHAFGSPLLTASDGSKFGKT
EGNAIWLDADLTSPWSFYQFFLNSDDADIPRLLRVFTFFTRSEIEDLNRSVRENASARLAHRHLAYSVTRLVHGADVADQ
VLLACSVLFGDGGALGKTQTGLPRIDSDTLNDSSGVTTIKAQDNTYTPYSPEGVRFAGTFPVDPRFLRSILFELPNATVS
AHDLITHILQKVGLCRSLSEARRLISQGGIYVNNIKVTCPDALLDSFLDNSMPIRAAILRKGKKHLAAVFY

Sequences:

>Translated_471_residues
MPNAPEVNSVWDELLWRGLVCVSTDQGALKELLDGPPIRFYCGFDPTAPSLHVGNLAQILIMRRLQLAGHRPIALVGGST
GLIGDPRPGGERQLHSHEQVQEWVRALQQQLSRFLDFKGAAAAVLVNNLDWTKSLTALDFLRELGKHFRVNAMLKKDAVA
ARLSSTEGISYTEFSYQILQSLDFRQLYLDYKCVLQIGGSDQWGNITSGVDLIRKTEGAGVHAFGSPLLTASDGSKFGKT
EGNAIWLDADLTSPWSFYQFFLNSDDADIPRLLRVFTFFTRSEIEDLNRSVRENASARLAHRHLAYSVTRLVHGADVADQ
VLLACSVLFGDGGALGKTQTGLPRIDSDTLNDSSGVTTIKAQDNTYTPYSPEGVRFAGTFPVDPRFLRSILFELPNATVS
AHDLITHILQKVGLCRSLSEARRLISQGGIYVNNIKVTCPDALLDSFLDNSMPIRAAILRKGKKHLAAVFY
>Mature_470_residues
PNAPEVNSVWDELLWRGLVCVSTDQGALKELLDGPPIRFYCGFDPTAPSLHVGNLAQILIMRRLQLAGHRPIALVGGSTG
LIGDPRPGGERQLHSHEQVQEWVRALQQQLSRFLDFKGAAAAVLVNNLDWTKSLTALDFLRELGKHFRVNAMLKKDAVAA
RLSSTEGISYTEFSYQILQSLDFRQLYLDYKCVLQIGGSDQWGNITSGVDLIRKTEGAGVHAFGSPLLTASDGSKFGKTE
GNAIWLDADLTSPWSFYQFFLNSDDADIPRLLRVFTFFTRSEIEDLNRSVRENASARLAHRHLAYSVTRLVHGADVADQV
LLACSVLFGDGGALGKTQTGLPRIDSDTLNDSSGVTTIKAQDNTYTPYSPEGVRFAGTFPVDPRFLRSILFELPNATVSA
HDLITHILQKVGLCRSLSEARRLISQGGIYVNNIKVTCPDALLDSFLDNSMPIRAAILRKGKKHLAAVFY

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=304, Percent_Identity=41.1184210526316, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI1787925, Length=346, Percent_Identity=48.8439306358381, Blast_Score=320, Evalue=9e-89,
Organism=Caenorhabditis elegans, GI25145802, Length=299, Percent_Identity=38.4615384615385, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI25145805, Length=209, Percent_Identity=39.2344497607655, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6325160, Length=494, Percent_Identity=33.8056680161943, Blast_Score=246, Evalue=8e-66,
Organism=Drosophila melanogaster, GI20130383, Length=386, Percent_Identity=38.0829015544041, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_TROW8 (Q83HH5)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789530.1
- ProteinModelPortal:   Q83HH5
- SMR:   Q83HH5
- STRING:   Q83HH5
- GeneID:   1064809
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW603
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   WINQDDR
- PhylomeDB:   Q83HH5
- ProtClustDB:   PRK05912
- BioCyc:   TWHI218496:TW0575-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 51889; Mature: 51757

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 41-41 BINDING 176-176 BINDING 180-180 BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNAPEVNSVWDELLWRGLVCVSTDQGALKELLDGPPIRFYCGFDPTAPSLHVGNLAQIL
CCCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHH
IMRRLQLAGHRPIALVGGSTGLIGDPRPGGERQLHSHEQVQEWVRALQQQLSRFLDFKGA
HHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
AAAVLVNNLDWTKSLTALDFLRELGKHFRVNAMLKKDAVAARLSSTEGISYTEFSYQILQ
EEEEEEECCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
SLDFRQLYLDYKCVLQIGGSDQWGNITSGVDLIRKTEGAGVHAFGSPLLTASDGSKFGKT
CCCHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECCCCEEECCCCCCCCCC
EGNAIWLDADLTSPWSFYQFFLNSDDADIPRLLRVFTFFTRSEIEDLNRSVRENASARLA
CCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRHLAYSVTRLVHGADVADQVLLACSVLFGDGGALGKTQTGLPRIDSDTLNDSSGVTTIK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
AQDNTYTPYSPEGVRFAGTFPVDPRFLRSILFELPNATVSAHDLITHILQKVGLCRSLSE
ECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ARRLISQGGIYVNNIKVTCPDALLDSFLDNSMPIRAAILRKGKKHLAAVFY
HHHHHHHCCEEEEEEEEECHHHHHHHHHCCCCCHHHHHHHCCHHHHHEECC
>Mature Secondary Structure 
PNAPEVNSVWDELLWRGLVCVSTDQGALKELLDGPPIRFYCGFDPTAPSLHVGNLAQIL
CCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHH
IMRRLQLAGHRPIALVGGSTGLIGDPRPGGERQLHSHEQVQEWVRALQQQLSRFLDFKGA
HHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
AAAVLVNNLDWTKSLTALDFLRELGKHFRVNAMLKKDAVAARLSSTEGISYTEFSYQILQ
EEEEEEECCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
SLDFRQLYLDYKCVLQIGGSDQWGNITSGVDLIRKTEGAGVHAFGSPLLTASDGSKFGKT
CCCHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECCCCEEECCCCCCCCCC
EGNAIWLDADLTSPWSFYQFFLNSDDADIPRLLRVFTFFTRSEIEDLNRSVRENASARLA
CCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRHLAYSVTRLVHGADVADQVLLACSVLFGDGGALGKTQTGLPRIDSDTLNDSSGVTTIK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
AQDNTYTPYSPEGVRFAGTFPVDPRFLRSILFELPNATVSAHDLITHILQKVGLCRSLSE
ECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ARRLISQGGIYVNNIKVTCPDALLDSFLDNSMPIRAAILRKGKKHLAAVFY
HHHHHHHCCEEEEEEEEECHHHHHHHHHCCCCCHHHHHHHCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174