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The map label for this gene is ruvA

Identifier: 28572651

GI number: 28572651

Start: 541501

End: 542025

Strand: Reverse

Name: ruvA

Synonym: TW502

Alternate gene names: 28572651

Gene position: 542025-541501 (Counterclockwise)

Preceding gene: 28572652

Following gene: 28572650

Centisome position: 58.54

GC content: 46.86

Gene sequence:

>525_bases
ATGCATGGTATAGGCTTTTTGGTTCGTGTGCCACAATCTTTTAACCCTGAAGTAGATGCTGAGGTAAAACTTTATACCAG
TCTTCAAGTCCGAGAAGACTCGGTTTCGCTTTACGGTTTTGCATCTGTTCTGGAATGCACTGTTTTTGAACAGCTAATAA
CAATATCCGGTGTTGGCCCGCGGGTTGCGCTTGCCATCCTTTCGGTTTTGACCCCGGCAGAGGTTGCTGCGGCCGTTCTG
GAAGGTGATGACAAGCCCTTGCAGCGAGTGAGTGGTGTTGGAAAAAAGCTTGCCGGAACAATAGTGTTACAGCTTGCTGG
GAAATTGACTTCGGTCCCATTAGAGAACAGGAAACAGGAACAAGCGGTTGATAGAAGTGCCGAGATTGTCCAGGCTCTTA
TTGGGTTGGGCTGGCAGCGACAGGAATCTGCTGCGGCCGTGGAATCTGTTTTAGAAAAAGATCAGTCTTTGACTATGCCA
GAAATTTTGAGAAATGCCCTTAGATATCTTGCAAAACAGGAATAA

Upstream 100 bases:

>100_bases
AAGTGTCTGTATATGCATTGCTTGCACCGGAGGGCTTGCTTTGATCGCGTCACTTTCTGGATTGTTGGAGGCTGTACGTC
CCGGAAGTGTTGTTGTGAAC

Downstream 100 bases:

>100_bases
CCTCTTGGATGTGACGTCTGACGAAAAACAAACAGAAGGTGCTTTGCGCCCGAAACTTCTTGCTGAGTTTGTAGGGCAGA
ATAAGGTAAAGAATCAGCTG

Product: holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MHGIGFLVRVPQSFNPEVDAEVKLYTSLQVREDSVSLYGFASVLECTVFEQLITISGVGPRVALAILSVLTPAEVAAAVL
EGDDKPLQRVSGVGKKLAGTIVLQLAGKLTSVPLENRKQEQAVDRSAEIVQALIGLGWQRQESAAAVESVLEKDQSLTMP
EILRNALRYLAKQE

Sequences:

>Translated_174_residues
MHGIGFLVRVPQSFNPEVDAEVKLYTSLQVREDSVSLYGFASVLECTVFEQLITISGVGPRVALAILSVLTPAEVAAAVL
EGDDKPLQRVSGVGKKLAGTIVLQLAGKLTSVPLENRKQEQAVDRSAEIVQALIGLGWQRQESAAAVESVLEKDQSLTMP
EILRNALRYLAKQE
>Mature_174_residues
MHGIGFLVRVPQSFNPEVDAEVKLYTSLQVREDSVSLYGFASVLECTVFEQLITISGVGPRVALAILSVLTPAEVAAAVL
EGDDKPLQRVSGVGKKLAGTIVLQLAGKLTSVPLENRKQEQAVDRSAEIVQALIGLGWQRQESAAAVESVLEKDQSLTMP
EILRNALRYLAKQE

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=180, Percent_Identity=31.1111111111111, Blast_Score=81, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_TROWT (Q83GK0)

Other databases:

- EMBL:   AE014184
- RefSeq:   NP_787396.1
- ProteinModelPortal:   Q83GK0
- SMR:   Q83GK0
- STRING:   Q83GK0
- GeneID:   1089518
- GenomeReviews:   AE014184_GR
- KEGG:   twh:TWT268
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   CLSK229871
- BioCyc:   TWHI203267:TW268-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 18797; Mature: 18797

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGIGFLVRVPQSFNPEVDAEVKLYTSLQVREDSVSLYGFASVLECTVFEQLITISGVGP
CCCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
RVALAILSVLTPAEVAAAVLEGDDKPLQRVSGVGKKLAGTIVLQLAGKLTSVPLENRKQE
HHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
QAVDRSAEIVQALIGLGWQRQESAAAVESVLEKDQSLTMPEILRNALRYLAKQE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHGIGFLVRVPQSFNPEVDAEVKLYTSLQVREDSVSLYGFASVLECTVFEQLITISGVGP
CCCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
RVALAILSVLTPAEVAAAVLEGDDKPLQRVSGVGKKLAGTIVLQLAGKLTSVPLENRKQE
HHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
QAVDRSAEIVQALIGLGWQRQESAAAVESVLEKDQSLTMPEILRNALRYLAKQE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12902375