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The map label for this gene is ruvB [H]

Identifier: 28572650

GI number: 28572650

Start: 540495

End: 541490

Strand: Reverse

Name: ruvB [H]

Synonym: TW501

Alternate gene names: 28572650

Gene position: 541490-540495 (Counterclockwise)

Preceding gene: 28572651

Following gene: 28572649

Centisome position: 58.48

GC content: 48.29

Gene sequence:

>996_bases
GTGACGTCTGACGAAAAACAAACAGAAGGTGCTTTGCGCCCGAAACTTCTTGCTGAGTTTGTAGGGCAGAATAAGGTAAA
GAATCAGCTGGCCCTTTTGATACAGGCAGCAAAAATTCAGGGGCGTGTTACCGATCATGCACTTTTAGCGGGACCCCCAG
GCCTTGGAAAAACAACTCTTGCAATGATTGTTGCCGCTGAATGTGGGGTTTCAATACGAATGTCAAGCGGCCCTGCCATA
CAGCATGCCGGAGATCTTGCTGCTCTTTTATCTAGCCTCTTGCCCGGAGAACTTCTCTTTATCGACGAAATTCACAGGAT
GAGTAGGGTCGCTGAGGAGATGCTCTATCTTGCCATGGAAGATTTTCGCATTGATATTATGGTTGGCAAGGGTCCTGGTG
CTACGAGCGTTCCCCTTGAATTGTCCCCTTTTACGCTGGTCGGCGCAACCACCAGAGCAGGTCTCTTGCCTGGACCATTA
CGGGATCGTTTTGGGTTTACTGCACGCCTCGATTTCTATTCTCCAGAAGAGCTTTTGCAGGTCTTGATTCGGAGTGCACG
ACTTATGGAGATTCAGTATTACGACGATGCCCTGGAAAGTATTGCCGTCAGGAGTCGAGGAACGCCTCGTGTTGCGAATC
GTCTTTTGCGCAGAACGCGTGACTATTTGCTCGTATCGAATTCATCAGAAATTCTCTCAAAGGAGATAGCACTCAAAGCA
ATGGATGTTTATGAAGTCGATTCGTTGGGTCTTGACCGACTTGATAGGGCGGTGTTGCATGCTATCTTCGATCGTTTTTC
AGGAGGCCCGGTTGGAATAAAAACCCTTTCAGCCTATCTGGGCGAGGAGGCTGAAACTATTGAAAACTCTATAGAGCCGT
TTTTGGTTAGGCAGGGTCTCCTTGTCAGGACCCCCAGAGGCCGTCAGATTACCGATTTAGCCAGGAAACACATGGGTTTC
AAGGAAGATCTATCTGGCTTTGAGTTATACTTATGA

Upstream 100 bases:

>100_bases
GCCGTGGAATCTGTTTTAGAAAAAGATCAGTCTTTGACTATGCCAGAAATTTTGAGAAATGCCCTTAGATATCTTGCAAA
ACAGGAATAACCTCTTGGAT

Downstream 100 bases:

>100_bases
GGTTCGTCTTAGAAGGTTGTCATGTCTCTTGAGTTATTGTTCCCCATTGCCCTTGTTCTACTCCTGGTTGCTTTTTTCTT
TTCGCAAATGAAAAGAAAAA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTSDEKQTEGALRPKLLAEFVGQNKVKNQLALLIQAAKIQGRVTDHALLAGPPGLGKTTLAMIVAAECGVSIRMSSGPAI
QHAGDLAALLSSLLPGELLFIDEIHRMSRVAEEMLYLAMEDFRIDIMVGKGPGATSVPLELSPFTLVGATTRAGLLPGPL
RDRFGFTARLDFYSPEELLQVLIRSARLMEIQYYDDALESIAVRSRGTPRVANRLLRRTRDYLLVSNSSEILSKEIALKA
MDVYEVDSLGLDRLDRAVLHAIFDRFSGGPVGIKTLSAYLGEEAETIENSIEPFLVRQGLLVRTPRGRQITDLARKHMGF
KEDLSGFELYL

Sequences:

>Translated_331_residues
MTSDEKQTEGALRPKLLAEFVGQNKVKNQLALLIQAAKIQGRVTDHALLAGPPGLGKTTLAMIVAAECGVSIRMSSGPAI
QHAGDLAALLSSLLPGELLFIDEIHRMSRVAEEMLYLAMEDFRIDIMVGKGPGATSVPLELSPFTLVGATTRAGLLPGPL
RDRFGFTARLDFYSPEELLQVLIRSARLMEIQYYDDALESIAVRSRGTPRVANRLLRRTRDYLLVSNSSEILSKEIALKA
MDVYEVDSLGLDRLDRAVLHAIFDRFSGGPVGIKTLSAYLGEEAETIENSIEPFLVRQGLLVRTPRGRQITDLARKHMGF
KEDLSGFELYL
>Mature_330_residues
TSDEKQTEGALRPKLLAEFVGQNKVKNQLALLIQAAKIQGRVTDHALLAGPPGLGKTTLAMIVAAECGVSIRMSSGPAIQ
HAGDLAALLSSLLPGELLFIDEIHRMSRVAEEMLYLAMEDFRIDIMVGKGPGATSVPLELSPFTLVGATTRAGLLPGPLR
DRFGFTARLDFYSPEELLQVLIRSARLMEIQYYDDALESIAVRSRGTPRVANRLLRRTRDYLLVSNSSEILSKEIALKAM
DVYEVDSLGLDRLDRAVLHAIFDRFSGGPVGIKTLSAYLGEEAETIENSIEPFLVRQGLLVRTPRGRQITDLARKHMGFK
EDLSGFELYL

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=319, Percent_Identity=54.2319749216301, Blast_Score=350, Evalue=1e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 36394; Mature: 36263

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDEKQTEGALRPKLLAEFVGQNKVKNQLALLIQAAKIQGRVTDHALLAGPPGLGKTTL
CCCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHH
AMIVAAECGVSIRMSSGPAIQHAGDLAALLSSLLPGELLFIDEIHRMSRVAEEMLYLAME
HHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHH
DFRIDIMVGKGPGATSVPLELSPFTLVGATTRAGLLPGPLRDRFGFTARLDFYSPEELLQ
CEEEEEEEECCCCCCCCCEEECCEEEEECCHHCCCCCCCHHHHCCCEEEECCCCHHHHHH
VLIRSARLMEIQYYDDALESIAVRSRGTPRVANRLLRRTRDYLLVSNSSEILSKEIALKA
HHHHHHHHEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHH
MDVYEVDSLGLDRLDRAVLHAIFDRFSGGPVGIKTLSAYLGEEAETIENSIEPFLVRQGL
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHCCCHHHHHCCC
LVRTPRGRQITDLARKHMGFKEDLSGFELYL
EEECCCCCHHHHHHHHHCCCHHCCCCCEECC
>Mature Secondary Structure 
TSDEKQTEGALRPKLLAEFVGQNKVKNQLALLIQAAKIQGRVTDHALLAGPPGLGKTTL
CCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHH
AMIVAAECGVSIRMSSGPAIQHAGDLAALLSSLLPGELLFIDEIHRMSRVAEEMLYLAME
HHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHH
DFRIDIMVGKGPGATSVPLELSPFTLVGATTRAGLLPGPLRDRFGFTARLDFYSPEELLQ
CEEEEEEEECCCCCCCCCEEECCEEEEECCHHCCCCCCCHHHHCCCEEEECCCCHHHHHH
VLIRSARLMEIQYYDDALESIAVRSRGTPRVANRLLRRTRDYLLVSNSSEILSKEIALKA
HHHHHHHHEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHH
MDVYEVDSLGLDRLDRAVLHAIFDRFSGGPVGIKTLSAYLGEEAETIENSIEPFLVRQGL
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHCCCHHHHHCCC
LVRTPRGRQITDLARKHMGFKEDLSGFELYL
EEECCCCCHHHHHHHHHCCCHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174 [H]