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The map label for this gene is polA [H]

Identifier: 28572629

GI number: 28572629

Start: 517394

End: 518464

Strand: Reverse

Name: polA [H]

Synonym: TW480

Alternate gene names: 28572629

Gene position: 518464-517394 (Counterclockwise)

Preceding gene: 28572630

Following gene: 28572628

Centisome position: 55.99

GC content: 43.6

Gene sequence:

>1071_bases
TTGACGGGCACACCAGACCAAAGTAAGAGACTTATTTTAGTAGATGGCCACTCTCTTGCTTTTAGATCCTTTTACGGAAC
GAAAGCGATTGCGGTAGTCAACGGGGTGCAGGTGAATGCTCTTTATGGCTTCGTTAATCGCCTCACCCAGCTTCTTGAAT
CAGGGCCTGTCAGTCATTTTGCAGTTGCGTTTGATGTATCGAGGAAATCCTTTCGAACCGAGGCGTATCCAGAGTATAAA
GGCACCCGTAAAAAAGTACCTGAGGAATTTTTTCCGCAAGTTGATCTGATAAAGGAATTTCTGCATCTTATTGGGGTGAA
TCATTTTGCAGTCCAGAATTATGAGGCCGATGATATAATCGCGACTCTGTCTCGTTTAGCCCTTGAAGAAGGATTTAGTG
ACGTAAGAATCCTGTCGGGGGATAGGGATCTTATTCAGCTCGTGAATAATAAAGTTTCTCTATTATTTTTCTCAGCAAAG
CACAGATTGGGATTTGCAGTCTACACACCTGAAGCAGTTATGGATAGGTATGGCATAGAACCGCACGTCTATCCGGATAT
GGCAGCACTGGTTGGTGAGTCAAGTGATAATCTGCCGGGCATAAAAAAAATTGGTGAAAAAACAGCCACCACTCTTTTGC
AGAAATACGGGTGTCTTCAGAAGATTATTGAGTCTCGCTGGGAAATATCAGGAGTTGTTGGGCAAAATATACGAAACGAC
TATCATGTCGCACTCAGAAATCGTAAGCTAAATACGCTTGTTAATGATTTGCCTTTGCCAGCTAGCGTATCTGATCTGTC
ACTTTCCGGAGAGATTGATACAAAGAAACTCGAGGGGTTTCTTCGAAGGTATAGAATGAATTCGATTACACCAAGACTTC
TTCGGTGCCTACAGAAATTCACTAATGCAGCAAATTTGAACAGAGCTTTAACTGCGTCACCGCAAAGGCGTGGATCGCCG
AGCATTCGGAAATATAATTCCAAAACTCAAAAGCCCGTCAGAAATAACCGCGTCTCGGGTGTTAGAAGGAGGCGATATAG
GGCTGACAGGACTTCCGTACAGCTTTTTTGA

Upstream 100 bases:

>100_bases
GTGGATACAGAAAATGTCAATGAATCGGAGAATTAGCATGAAAGAAGTTTCAAAGAGCATCATTGCGCAATTAGCCGGCA
AATAGAACAGTTATAAAAGC

Downstream 100 bases:

>100_bases
GACTTGTCTTATCCCTCTGGGCATCTATTGAATTTTTAGCTAAATATGCTGGAATGTACTCTTAAAGTGTCGGAGTTGTG
AATGATAGCCGTTTGCTGCT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTGTPDQSKRLILVDGHSLAFRSFYGTKAIAVVNGVQVNALYGFVNRLTQLLESGPVSHFAVAFDVSRKSFRTEAYPEYK
GTRKKVPEEFFPQVDLIKEFLHLIGVNHFAVQNYEADDIIATLSRLALEEGFSDVRILSGDRDLIQLVNNKVSLLFFSAK
HRLGFAVYTPEAVMDRYGIEPHVYPDMAALVGESSDNLPGIKKIGEKTATTLLQKYGCLQKIIESRWEISGVVGQNIRND
YHVALRNRKLNTLVNDLPLPASVSDLSLSGEIDTKKLEGFLRRYRMNSITPRLLRCLQKFTNAANLNRALTASPQRRGSP
SIRKYNSKTQKPVRNNRVSGVRRRRYRADRTSVQLF

Sequences:

>Translated_356_residues
MTGTPDQSKRLILVDGHSLAFRSFYGTKAIAVVNGVQVNALYGFVNRLTQLLESGPVSHFAVAFDVSRKSFRTEAYPEYK
GTRKKVPEEFFPQVDLIKEFLHLIGVNHFAVQNYEADDIIATLSRLALEEGFSDVRILSGDRDLIQLVNNKVSLLFFSAK
HRLGFAVYTPEAVMDRYGIEPHVYPDMAALVGESSDNLPGIKKIGEKTATTLLQKYGCLQKIIESRWEISGVVGQNIRND
YHVALRNRKLNTLVNDLPLPASVSDLSLSGEIDTKKLEGFLRRYRMNSITPRLLRCLQKFTNAANLNRALTASPQRRGSP
SIRKYNSKTQKPVRNNRVSGVRRRRYRADRTSVQLF
>Mature_355_residues
TGTPDQSKRLILVDGHSLAFRSFYGTKAIAVVNGVQVNALYGFVNRLTQLLESGPVSHFAVAFDVSRKSFRTEAYPEYKG
TRKKVPEEFFPQVDLIKEFLHLIGVNHFAVQNYEADDIIATLSRLALEEGFSDVRILSGDRDLIQLVNNKVSLLFFSAKH
RLGFAVYTPEAVMDRYGIEPHVYPDMAALVGESSDNLPGIKKIGEKTATTLLQKYGCLQKIIESRWEISGVVGQNIRNDY
HVALRNRKLNTLVNDLPLPASVSDLSLSGEIDTKKLEGFLRRYRMNSITPRLLRCLQKFTNAANLNRALTASPQRRGSPS
IRKYNSKTQKPVRNNRVSGVRRRRYRADRTSVQLF

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=287, Percent_Identity=32.0557491289199, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI226510970, Length=260, Percent_Identity=25.7692307692308, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40222; Mature: 40091

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTPDQSKRLILVDGHSLAFRSFYGTKAIAVVNGVQVNALYGFVNRLTQLLESGPVSHF
CCCCCCCCCEEEEEECCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCE
AVAFDVSRKSFRTEAYPEYKGTRKKVPEEFFPQVDLIKEFLHLIGVNHFAVQNYEADDII
EEEEEHHHHHHCCCCCCCCCCHHHCCCHHHCCCHHHHHHHHHHHCCCHHEECCCCHHHHH
ATLSRLALEEGFSDVRILSGDRDLIQLVNNKVSLLFFSAKHRLGFAVYTPEAVMDRYGIE
HHHHHHHHHCCCCCEEEECCCHHHHHHHCCCEEEEEEECCCCCEEEEECHHHHHHHCCCC
PHVYPDMAALVGESSDNLPGIKKIGEKTATTLLQKYGCLQKIIESRWEISGVVGQNIRND
CCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YHVALRNRKLNTLVNDLPLPASVSDLSLSGEIDTKKLEGFLRRYRMNSITPRLLRCLQKF
EEEEEECCHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
TNAANLNRALTASPQRRGSPSIRKYNSKTQKPVRNNRVSGVRRRRYRADRTSVQLF
HHHHCCCHHHCCCCCCCCCCCHHHCCCHHCCCHHCCHHHHHHHHHHHCCCCCEECC
>Mature Secondary Structure 
TGTPDQSKRLILVDGHSLAFRSFYGTKAIAVVNGVQVNALYGFVNRLTQLLESGPVSHF
CCCCCCCCEEEEEECCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCE
AVAFDVSRKSFRTEAYPEYKGTRKKVPEEFFPQVDLIKEFLHLIGVNHFAVQNYEADDII
EEEEEHHHHHHCCCCCCCCCCHHHCCCHHHCCCHHHHHHHHHHHCCCHHEECCCCHHHHH
ATLSRLALEEGFSDVRILSGDRDLIQLVNNKVSLLFFSAKHRLGFAVYTPEAVMDRYGIE
HHHHHHHHHCCCCCEEEECCCHHHHHHHCCCEEEEEEECCCCCEEEEECHHHHHHHCCCC
PHVYPDMAALVGESSDNLPGIKKIGEKTATTLLQKYGCLQKIIESRWEISGVVGQNIRND
CCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YHVALRNRKLNTLVNDLPLPASVSDLSLSGEIDTKKLEGFLRRYRMNSITPRLLRCLQKF
EEEEEECCHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
TNAANLNRALTASPQRRGSPSIRKYNSKTQKPVRNNRVSGVRRRRYRADRTSVQLF
HHHHCCCHHHCCCCCCCCCCCHHHCCCHHCCCHHCCHHHHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7476188; 11234002 [H]