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The map label for this gene is hdhA [C]

Identifier: 28572600

GI number: 28572600

Start: 488661

End: 489437

Strand: Reverse

Name: hdhA [C]

Synonym: TW450

Alternate gene names: 28572600

Gene position: 489437-488661 (Counterclockwise)

Preceding gene: 28572601

Following gene: 28572596

Centisome position: 52.86

GC content: 44.53

Gene sequence:

>777_bases
GTGACTGACTTGGGATTGATTGTCTTTGATTCTGATGTTTTACGCGATAAAAACGTCCTTATCACCGGTGGTTCAAGGGG
TATCGGTGCTAAAACCGCATATTTTCTTGCTCAAGGGGGAGCTCAAGTTTCCATTGTTTACAGGAATGGTGCCGCGCGAG
CTGATAAGGTCCTTCATGAAATAGAATCTGGTACAGGCATTAAAGGTTGTGCGGTTCGGGCTGATATAACTACAGATCAG
GATGTTCTGCGGCAGTTTTTAAAAGAGAAAGTAGAAAAGCTCGACATACTCATTCTGAATGCATCGGGTGGCATGGAATC
TGGCATGCCCGGCGATTATCCTATGAGGTTAAATCGAGATGCTCAACTAAATACCGTGGAGACCTGTCTTCCATTTATGC
CAGCTGGCTCCAGGGTGGTGTTTGTAACAAGTCACCAGGCGCATTTTATAACTACCTGCCAGACTCTTCCTGAGTACGAA
AAAGTTGCGCTATCAAAACGCGCCGGGGAAGATGCTCTCGTGCAAAAAATGAAAGATATACCATATATAGGTTTTGTTGT
TGTCTCTGGGGATATGATAGTCGGCACAGTTACAGCAATGCTTATCAATAGATTGTATCCCGGTGCGCTAGATGAGCGTA
GGAATAAGACTGGTAAGCTGTACGATGTATCAGAATTTGCGGCGGAAGTAGCCAGAGCAGCAATTGACCCAATTCCAGAA
GGCAATATCAAATTTGTTGGCGATATAAGTACATTTCTGGCAGATACAGAAGGCTAA

Upstream 100 bases:

>100_bases
ATGTTGTTTCTATGTTTCTGCTTGCCGCACCTGTGATTTTTCTGTACGGTTTTGCTTGTCTCATTTGTTTTGGTTATGAA
AAAATTAGGGATTCTCTGTC

Downstream 100 bases:

>100_bases
TCAATCGCTCAGGCTGTTGTATATATCACTGCAGGTCTGACAGATTGGTAGTTTGTCAGGTAGCCTGGAAGGAAGCCACC
TCTTCCCACACAGGGCAATG

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: Short-Chain Dehydrogenase/Reductase SDR; Short-Chain Dehydrogenases/Reductases Family Protein; Glucose-1-Dehydrogenase; 3-Oxoacyl-[Acyl-Carrier Protein] Reductase; Dehydrogenase; Short-Chain Alcohol Dehydrogenase Like Protein; Oxidoreductase Short Chain Dehydrogenase/Reductase Family; Short-Chain Dehydrogenase/Oxidoreductase

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTDLGLIVFDSDVLRDKNVLITGGSRGIGAKTAYFLAQGGAQVSIVYRNGAARADKVLHEIESGTGIKGCAVRADITTDQ
DVLRQFLKEKVEKLDILILNASGGMESGMPGDYPMRLNRDAQLNTVETCLPFMPAGSRVVFVTSHQAHFITTCQTLPEYE
KVALSKRAGEDALVQKMKDIPYIGFVVVSGDMIVGTVTAMLINRLYPGALDERRNKTGKLYDVSEFAAEVARAAIDPIPE
GNIKFVGDISTFLADTEG

Sequences:

>Translated_258_residues
MTDLGLIVFDSDVLRDKNVLITGGSRGIGAKTAYFLAQGGAQVSIVYRNGAARADKVLHEIESGTGIKGCAVRADITTDQ
DVLRQFLKEKVEKLDILILNASGGMESGMPGDYPMRLNRDAQLNTVETCLPFMPAGSRVVFVTSHQAHFITTCQTLPEYE
KVALSKRAGEDALVQKMKDIPYIGFVVVSGDMIVGTVTAMLINRLYPGALDERRNKTGKLYDVSEFAAEVARAAIDPIPE
GNIKFVGDISTFLADTEG
>Mature_257_residues
TDLGLIVFDSDVLRDKNVLITGGSRGIGAKTAYFLAQGGAQVSIVYRNGAARADKVLHEIESGTGIKGCAVRADITTDQD
VLRQFLKEKVEKLDILILNASGGMESGMPGDYPMRLNRDAQLNTVETCLPFMPAGSRVVFVTSHQAHFITTCQTLPEYEK
VALSKRAGEDALVQKMKDIPYIGFVVVSGDMIVGTVTAMLINRLYPGALDERRNKTGKLYDVSEFAAEVARAAIDPIPEG
NIKFVGDISTFLADTEG

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 27892; Mature: 27761

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLGLIVFDSDVLRDKNVLITGGSRGIGAKTAYFLAQGGAQVSIVYRNGAARADKVLHE
CCCCEEEEECCCHHCCCCEEEECCCCCCCCHHEEEEECCCCEEEEEEECCCHHHHHHHHH
IESGTGIKGCAVRADITTDQDVLRQFLKEKVEKLDILILNASGGMESGMPGDYPMRLNRD
HHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCEEECCC
AQLNTVETCLPFMPAGSRVVFVTSHQAHFITTCQTLPEYEKVALSKRAGEDALVQKMKDI
CCCCHHHHHHCCCCCCCEEEEEECCCCEEEEHHHHCCHHHHHHHHHCCCHHHHHHHHHCC
PYIGFVVVSGDMIVGTVTAMLINRLYPGALDERRNKTGKLYDVSEFAAEVARAAIDPIPE
CEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCC
GNIKFVGDISTFLADTEG
CCEEEEECHHHHHCCCCC
>Mature Secondary Structure 
TDLGLIVFDSDVLRDKNVLITGGSRGIGAKTAYFLAQGGAQVSIVYRNGAARADKVLHE
CCCEEEEECCCHHCCCCEEEECCCCCCCCHHEEEEECCCCEEEEEEECCCHHHHHHHHH
IESGTGIKGCAVRADITTDQDVLRQFLKEKVEKLDILILNASGGMESGMPGDYPMRLNRD
HHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCEEECCC
AQLNTVETCLPFMPAGSRVVFVTSHQAHFITTCQTLPEYEKVALSKRAGEDALVQKMKDI
CCCCHHHHHHCCCCCCCEEEEEECCCCEEEEHHHHCCHHHHHHHHHCCCHHHHHHHHHCC
PYIGFVVVSGDMIVGTVTAMLINRLYPGALDERRNKTGKLYDVSEFAAEVARAAIDPIPE
CEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCC
GNIKFVGDISTFLADTEG
CCEEEEECHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA