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The map label for this gene is priA [H]

Identifier: 28572563

GI number: 28572563

Start: 447831

End: 449705

Strand: Direct

Name: priA [H]

Synonym: TW410

Alternate gene names: 28572563

Gene position: 447831-449705 (Clockwise)

Preceding gene: 28572562

Following gene: 28572564

Centisome position: 48.37

GC content: 42.19

Gene sequence:

>1875_bases
GTGCCGCGTGTAGCAAAAGTTCTTCTGAATTTATCGGGACGAGATATTCTGCTGGACTATTTTGTACCAGATGGTTTACA
TATAAACAGAGGGCATCTCGTAACTGTTCCTTTCAGGGGCAAAATCTGTGAGGGTTATGTCTTGAACATACACACTCCAG
AATCTTACGTAGGACATTTGCGTTTTATCCATGATAAGCCCTTGGAAATACCTGTTCTAACACGTGATATAAGCCTTTTG
GCTCACAGGATTGCGCGAAGAAACTGCAGTCAGATTGGTAATTTGCTGCATCTTGCAATTCCTAAAAGAAGAGTCAGGGT
AGAAAAAGCCTTCTCTAAAAATCTGTCCGGCATAAAGGATACGGATCCACCAGTCCAGAGTCAAGATTTTGCCCTTTTCA
GTCAGCGCGTGCGCTTGACCTCACGGTGCAGGTATTCATTGTGCGTCCCGATAGGTATCGATACTCAGGGTATTCAGCCG
TGGGCCCACGAATTTGCTGCCCTTGCCACTCAATGTTATCGGTCTGGTAGAACAGCAATTCTTGCTGTTCCAGATCAAAA
ATGTCAGAGGCAACTCATAAAGGCACTGTCAAAGGTGCCTGAATTACGGAATGATGATATAAAGATTCTTAATTCAAATC
AAACTTCGTCCGATAGATATAAAAACTTTCTTGATTGCCTCCTTGATAGGGAGAGAATCATTGTTGGGAACCGTTCTGTT
ATTTATGCGCCCAGCAACAATCTAGGGCTTATAGCAATTTGGGATGAGGCAAATTATGCATTTCTTGAACACAGAACTCC
ATATCCACATATAAGAGATATTGCACTAATAAGACAACAAATCTCCGGCTGTGTTTTATTATTTGCTTGTCATATCCCAA
GCATGCCGGTTATGTATCTGGAAAAAACCGGTCATATAGAATGTGTCGGATACGCATATCGAAGAAGGCCAAAACTTTTT
GTACCGAAAGAACCCGGGCAGATTCCATCTGATGCCATAAGAGTTATGAGAAACGCTTTGGATACGGGACCCGTTTTGGT
TCAGGTGCAACAGAAGGGTCATATTCAAAGCTTTTCCTGTGCAAATTGCGGCCAACTTGCCCGCTGTAAGGACTGCTCCG
GACCTCTCACAAAAACATCTTTTTACGGCACCTCTCAGTGTAAATGGTGTGCCAAAATTGCCGATAACGCATGTTCAATT
TGTGGATCACAAAAAATTGTTATGCTCAGAAGGGGCAGTGGTAGAACTGCATATGAATTAACCCAGGCATTTCCAGGGTG
TAACGTGCTAACAAGCGATTCATCGAATCCGATTGAGTTACTTGATGTCAAAATGCTTAAAGGCACTATTGTTGTTTCGA
CTTGCGGTAGCGAGCCGTTAGTTTCGCAGGGCTATGCTGCTGTTTTAATTCTTGATGCAAACACAATGGCCGTTAGGCAA
AACTTTGAGACTGCCTACTCGACTCTAAAAAGGTGGACTGCTGCAATTTCCCTATTACGACATGATGGAACTCTTATAAT
TCCAGGTATTGTAAATAGATTAGTGAGAGCGATCGGCGCTTGGCAACTTGTGAGCTATCTGGAAACAGAGTTGGAGCATA
GGATTACCCTAAAATTACCTCCGGCTGTGAGAATTTTCAGTATAACTGGTCGTAAAAGTGAAATTGATATTATCGCTTTG
CATCTCAGGGAATTTGATCCTGTTAGGGTTTTTGGCCCTATAAGCCAGGATGATTCAAAATTTCGCTTGATTGTTATGAA
CGATTTTAAGGATGTCAATAGAGTGATGGATTTTGTGAAAAAAATGTTGGATTCTTTGCGTGGTAGCTCTATCAAAATTA
GATGTGACGACCCGGAAATTCTCGATGAAATCTAG

Upstream 100 bases:

>100_bases
ATATCAAAAAACGGCTGCATACGGCCATTTCGGTCGAGATATCTTTACCTGGGAGCGTATCTGTCCTGATAAGCTTAACG
CTCTTTTGGGTGCTGTTTAA

Downstream 100 bases:

>100_bases
TTTACGGATTGTATATGCTGGGACTTCAAGCGTTGCATTGCCAGCGTTGCGTTGCATAAAAACAAGCCAACATACTCTGG
TTGGAGTTTTAGCAAAGCGT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 624; Mature: 623

Protein sequence:

>624_residues
MPRVAKVLLNLSGRDILLDYFVPDGLHINRGHLVTVPFRGKICEGYVLNIHTPESYVGHLRFIHDKPLEIPVLTRDISLL
AHRIARRNCSQIGNLLHLAIPKRRVRVEKAFSKNLSGIKDTDPPVQSQDFALFSQRVRLTSRCRYSLCVPIGIDTQGIQP
WAHEFAALATQCYRSGRTAILAVPDQKCQRQLIKALSKVPELRNDDIKILNSNQTSSDRYKNFLDCLLDRERIIVGNRSV
IYAPSNNLGLIAIWDEANYAFLEHRTPYPHIRDIALIRQQISGCVLLFACHIPSMPVMYLEKTGHIECVGYAYRRRPKLF
VPKEPGQIPSDAIRVMRNALDTGPVLVQVQQKGHIQSFSCANCGQLARCKDCSGPLTKTSFYGTSQCKWCAKIADNACSI
CGSQKIVMLRRGSGRTAYELTQAFPGCNVLTSDSSNPIELLDVKMLKGTIVVSTCGSEPLVSQGYAAVLILDANTMAVRQ
NFETAYSTLKRWTAAISLLRHDGTLIIPGIVNRLVRAIGAWQLVSYLETELEHRITLKLPPAVRIFSITGRKSEIDIIAL
HLREFDPVRVFGPISQDDSKFRLIVMNDFKDVNRVMDFVKKMLDSLRGSSIKIRCDDPEILDEI

Sequences:

>Translated_624_residues
MPRVAKVLLNLSGRDILLDYFVPDGLHINRGHLVTVPFRGKICEGYVLNIHTPESYVGHLRFIHDKPLEIPVLTRDISLL
AHRIARRNCSQIGNLLHLAIPKRRVRVEKAFSKNLSGIKDTDPPVQSQDFALFSQRVRLTSRCRYSLCVPIGIDTQGIQP
WAHEFAALATQCYRSGRTAILAVPDQKCQRQLIKALSKVPELRNDDIKILNSNQTSSDRYKNFLDCLLDRERIIVGNRSV
IYAPSNNLGLIAIWDEANYAFLEHRTPYPHIRDIALIRQQISGCVLLFACHIPSMPVMYLEKTGHIECVGYAYRRRPKLF
VPKEPGQIPSDAIRVMRNALDTGPVLVQVQQKGHIQSFSCANCGQLARCKDCSGPLTKTSFYGTSQCKWCAKIADNACSI
CGSQKIVMLRRGSGRTAYELTQAFPGCNVLTSDSSNPIELLDVKMLKGTIVVSTCGSEPLVSQGYAAVLILDANTMAVRQ
NFETAYSTLKRWTAAISLLRHDGTLIIPGIVNRLVRAIGAWQLVSYLETELEHRITLKLPPAVRIFSITGRKSEIDIIAL
HLREFDPVRVFGPISQDDSKFRLIVMNDFKDVNRVMDFVKKMLDSLRGSSIKIRCDDPEILDEI
>Mature_623_residues
PRVAKVLLNLSGRDILLDYFVPDGLHINRGHLVTVPFRGKICEGYVLNIHTPESYVGHLRFIHDKPLEIPVLTRDISLLA
HRIARRNCSQIGNLLHLAIPKRRVRVEKAFSKNLSGIKDTDPPVQSQDFALFSQRVRLTSRCRYSLCVPIGIDTQGIQPW
AHEFAALATQCYRSGRTAILAVPDQKCQRQLIKALSKVPELRNDDIKILNSNQTSSDRYKNFLDCLLDRERIIVGNRSVI
YAPSNNLGLIAIWDEANYAFLEHRTPYPHIRDIALIRQQISGCVLLFACHIPSMPVMYLEKTGHIECVGYAYRRRPKLFV
PKEPGQIPSDAIRVMRNALDTGPVLVQVQQKGHIQSFSCANCGQLARCKDCSGPLTKTSFYGTSQCKWCAKIADNACSIC
GSQKIVMLRRGSGRTAYELTQAFPGCNVLTSDSSNPIELLDVKMLKGTIVVSTCGSEPLVSQGYAAVLILDANTMAVRQN
FETAYSTLKRWTAAISLLRHDGTLIIPGIVNRLVRAIGAWQLVSYLETELEHRITLKLPPAVRIFSITGRKSEIDIIALH
LREFDPVRVFGPISQDDSKFRLIVMNDFKDVNRVMDFVKKMLDSLRGSSIKIRCDDPEILDEI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=266, Percent_Identity=21.8045112781955, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 70115; Mature: 69983

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRVAKVLLNLSGRDILLDYFVPDGLHINRGHLVTVPFRGKICEGYVLNIHTPESYVGHL
CCHHHHHHHCCCCCEEEEEEECCCCEEECCCCEEEEECCCCEECCEEEEEECCHHHHCCE
RFIHDKPLEIPVLTRDISLLAHRIARRNCSQIGNLLHLAIPKRRVRVEKAFSKNLSGIKD
EEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCCC
TDPPVQSQDFALFSQRVRLTSRCRYSLCVPIGIDTQGIQPWAHEFAALATQCYRSGRTAI
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEE
LAVPDQKCQRQLIKALSKVPELRNDDIKILNSNQTSSDRYKNFLDCLLDRERIIVGNRSV
EECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCEE
IYAPSNNLGLIAIWDEANYAFLEHRTPYPHIRDIALIRQQISGCVLLFACHIPSMPVMYL
EEECCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEEE
EKTGHIECVGYAYRRRPKLFVPKEPGQIPSDAIRVMRNALDTGPVLVQVQQKGHIQSFSC
ECCCCEEEEEHHHHCCCCEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
ANCGQLARCKDCSGPLTKTSFYGTSQCKWCAKIADNACSICGSQKIVMLRRGSGRTAYEL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
TQAFPGCNVLTSDSSNPIELLDVKMLKGTIVVSTCGSEPLVSQGYAAVLILDANTMAVRQ
HHHCCCCCEEECCCCCCEEEEEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHH
NFETAYSTLKRWTAAISLLRHDGTLIIPGIVNRLVRAIGAWQLVSYLETELEHRITLKLP
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECC
PAVRIFSITGRKSEIDIIALHLREFDPVRVFGPISQDDSKFRLIVMNDFKDVNRVMDFVK
CCEEEEEECCCCCCEEEEEEEECCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHH
KMLDSLRGSSIKIRCDDPEILDEI
HHHHHHCCCEEEEEECCHHHHHCC
>Mature Secondary Structure 
PRVAKVLLNLSGRDILLDYFVPDGLHINRGHLVTVPFRGKICEGYVLNIHTPESYVGHL
CHHHHHHHCCCCCEEEEEEECCCCEEECCCCEEEEECCCCEECCEEEEEECCHHHHCCE
RFIHDKPLEIPVLTRDISLLAHRIARRNCSQIGNLLHLAIPKRRVRVEKAFSKNLSGIKD
EEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCCC
TDPPVQSQDFALFSQRVRLTSRCRYSLCVPIGIDTQGIQPWAHEFAALATQCYRSGRTAI
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEE
LAVPDQKCQRQLIKALSKVPELRNDDIKILNSNQTSSDRYKNFLDCLLDRERIIVGNRSV
EECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEECCCEE
IYAPSNNLGLIAIWDEANYAFLEHRTPYPHIRDIALIRQQISGCVLLFACHIPSMPVMYL
EEECCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEEE
EKTGHIECVGYAYRRRPKLFVPKEPGQIPSDAIRVMRNALDTGPVLVQVQQKGHIQSFSC
ECCCCEEEEEHHHHCCCCEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
ANCGQLARCKDCSGPLTKTSFYGTSQCKWCAKIADNACSICGSQKIVMLRRGSGRTAYEL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
TQAFPGCNVLTSDSSNPIELLDVKMLKGTIVVSTCGSEPLVSQGYAAVLILDANTMAVRQ
HHHCCCCCEEECCCCCCEEEEEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHH
NFETAYSTLKRWTAAISLLRHDGTLIIPGIVNRLVRAIGAWQLVSYLETELEHRITLKLP
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECC
PAVRIFSITGRKSEIDIIALHLREFDPVRVFGPISQDDSKFRLIVMNDFKDVNRVMDFVK
CCEEEEEECCCCCCEEEEEEEECCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHH
KMLDSLRGSSIKIRCDDPEILDEI
HHHHHHCCCEEEEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]