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The map label for this gene is metK

Identifier: 28572562

GI number: 28572562

Start: 446643

End: 447830

Strand: Direct

Name: metK

Synonym: TW409

Alternate gene names: 28572562

Gene position: 446643-447830 (Clockwise)

Preceding gene: 28572561

Following gene: 28572563

Centisome position: 48.24

GC content: 45.37

Gene sequence:

>1188_bases
GTGATTCTCACATCTGAATCTGTGACAGAAGGGCATCCGGATAAACTGTGTGATCAGATTTCTGATGCAATTCTTGATGG
TGTTATTTGCAAAGATAAGAATGCCCGTGCAGGTATTGAGACAATTGCAGGTAACGGCGTTGTGCATGTCTTCGGTGAAG
TATCAAATCCGGACTCTGTTGATATACCCGGGATAATCAGAAAGACAATCTTGGACATTGGTTATACCAGCGAAGACGCG
GGTATAGACGGTAATACGTGTTCGATACAGGAATCCATAACCTCTCAATCCAAGGAGATTGCAGATGCTGTAAACTTCTC
TCTCGAATATCGCAATCAACAGGGTGATTTGGGGCGAAACAGTTTTTCACAACAAGGCTCAGGCGACCAGGGAAGCGTCT
TTGGCTATGCGTGCAGGGAAACACCAGAGATGATGCCCTTGCCAATTACTATTGCGCATAAGCTTGCATACTCCTTAGCT
TTCGTGAGAAAAGAGAAGATTCTTCCGTATCTTTTGCCTGATGGAAAATCGCAGGTTACCCTAGGTTATGATTCGGCGAA
TAGACCCAAAACGCTTGAAACAGTCGTTATTTCGGCACAGCATGAAGATTCTGTTGACCTAGACAAACTGAGGTTTGACA
TTCTTGAGCGTGTTGTAAGACCGGTTATTTCAGCAACCGGTCTTGATTGTAGCAAAGCTACTTTTTTGATAAATCCGGCT
GGAAGATTTGTTACGGGGGGACCTTCCGCAGACTCTGGACTTACCGGTCGAAAGATTGTTGTTGATACGTATGGATGTGC
CGCAAAGCATGGTGGAGGTGCCCTTAGCGGTAAGGATCCATCAAAGCTTGATCGTTTCGCCTCCTATATGGCGAGATGGG
TTGCCAAACACGTCGTTGCGGCTGATTTTGCCGAATCGATTGAAGTACAGATTTCATACGCAATTGGAAAAGCCCATCCG
GTTGCGTTTAACATTGACACACACGGGACAAGCACCATCGCCTTAGATAAGCTCAAGTGTGCAATTCTAAAGGTATTTGA
CTTCAGGCCCGCTGCTGTTATAGATTCTCTCGATCTAAAAAGGCCCATATATCAAAAAACGGCTGCATACGGCCATTTCG
GTCGAGATATCTTTACCTGGGAGCGTATCTGTCCTGATAAGCTTAACGCTCTTTTGGGTGCTGTTTAA

Upstream 100 bases:

>100_bases
TAGTAAGGATTACATCGCAAAGCGTATCCTTGATTTCCTCTCCGAGAGAGTGATCGTTACATATATTGACCCTCTCGACA
GGTCGCTTTAAAGGTAAGTT

Downstream 100 bases:

>100_bases
GTGCCGCGTGTAGCAAAAGTTCTTCTGAATTTATCGGGACGAGATATTCTGCTGGACTATTTTGTACCAGATGGTTTACA
TATAAACAGAGGGCATCTCG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MILTSESVTEGHPDKLCDQISDAILDGVICKDKNARAGIETIAGNGVVHVFGEVSNPDSVDIPGIIRKTILDIGYTSEDA
GIDGNTCSIQESITSQSKEIADAVNFSLEYRNQQGDLGRNSFSQQGSGDQGSVFGYACRETPEMMPLPITIAHKLAYSLA
FVRKEKILPYLLPDGKSQVTLGYDSANRPKTLETVVISAQHEDSVDLDKLRFDILERVVRPVISATGLDCSKATFLINPA
GRFVTGGPSADSGLTGRKIVVDTYGCAAKHGGGALSGKDPSKLDRFASYMARWVAKHVVAADFAESIEVQISYAIGKAHP
VAFNIDTHGTSTIALDKLKCAILKVFDFRPAAVIDSLDLKRPIYQKTAAYGHFGRDIFTWERICPDKLNALLGAV

Sequences:

>Translated_395_residues
MILTSESVTEGHPDKLCDQISDAILDGVICKDKNARAGIETIAGNGVVHVFGEVSNPDSVDIPGIIRKTILDIGYTSEDA
GIDGNTCSIQESITSQSKEIADAVNFSLEYRNQQGDLGRNSFSQQGSGDQGSVFGYACRETPEMMPLPITIAHKLAYSLA
FVRKEKILPYLLPDGKSQVTLGYDSANRPKTLETVVISAQHEDSVDLDKLRFDILERVVRPVISATGLDCSKATFLINPA
GRFVTGGPSADSGLTGRKIVVDTYGCAAKHGGGALSGKDPSKLDRFASYMARWVAKHVVAADFAESIEVQISYAIGKAHP
VAFNIDTHGTSTIALDKLKCAILKVFDFRPAAVIDSLDLKRPIYQKTAAYGHFGRDIFTWERICPDKLNALLGAV
>Mature_395_residues
MILTSESVTEGHPDKLCDQISDAILDGVICKDKNARAGIETIAGNGVVHVFGEVSNPDSVDIPGIIRKTILDIGYTSEDA
GIDGNTCSIQESITSQSKEIADAVNFSLEYRNQQGDLGRNSFSQQGSGDQGSVFGYACRETPEMMPLPITIAHKLAYSLA
FVRKEKILPYLLPDGKSQVTLGYDSANRPKTLETVVISAQHEDSVDLDKLRFDILERVVRPVISATGLDCSKATFLINPA
GRFVTGGPSADSGLTGRKIVVDTYGCAAKHGGGALSGKDPSKLDRFASYMARWVAKHVVAADFAESIEVQISYAIGKAHP
VAFNIDTHGTSTIALDKLKCAILKVFDFRPAAVIDSLDLKRPIYQKTAAYGHFGRDIFTWERICPDKLNALLGAV

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=392, Percent_Identity=46.4285714285714, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI4557737, Length=391, Percent_Identity=46.5473145780051, Blast_Score=330, Evalue=2e-90,
Organism=Escherichia coli, GI1789311, Length=381, Percent_Identity=48.0314960629921, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17551082, Length=385, Percent_Identity=46.4935064935065, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17538498, Length=385, Percent_Identity=44.4155844155844, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI25145633, Length=385, Percent_Identity=43.8961038961039, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17538494, Length=385, Percent_Identity=43.8961038961039, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI32565905, Length=385, Percent_Identity=40.5194805194805, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI32565911, Length=339, Percent_Identity=43.3628318584071, Blast_Score=257, Evalue=6e-69,
Organism=Caenorhabditis elegans, GI25145635, Length=339, Percent_Identity=42.4778761061947, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI32565903, Length=312, Percent_Identity=43.9102564102564, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=42.1686746987952, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320710, Length=392, Percent_Identity=46.4285714285714, Blast_Score=333, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6323209, Length=385, Percent_Identity=45.974025974026, Blast_Score=323, Evalue=2e-89,
Organism=Drosophila melanogaster, GI45552159, Length=388, Percent_Identity=44.0721649484536, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24580529, Length=388, Percent_Identity=44.0721649484536, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24580537, Length=388, Percent_Identity=44.0721649484536, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24580533, Length=388, Percent_Identity=44.0721649484536, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24580535, Length=388, Percent_Identity=44.0721649484536, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24580531, Length=388, Percent_Identity=44.0721649484536, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24580527, Length=388, Percent_Identity=44.0721649484536, Blast_Score=301, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24580525, Length=388, Percent_Identity=44.0721649484536, Blast_Score=301, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=43.2, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24580539, Length=108, Percent_Identity=43.5185185185185, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_TROW8 (Q83HT7)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789342.1
- ProteinModelPortal:   Q83HT7
- SMR:   Q83HT7
- STRING:   Q83HT7
- GeneID:   1064253
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW409
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   TWHI218496:TW0387-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42585; Mature: 42585

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILTSESVTEGHPDKLCDQISDAILDGVICKDKNARAGIETIAGNGVVHVFGEVSNPDSV
CEECCCCCCCCCHHHHHHHHHHHHHHCEEECCCCCCCCHHHHCCCCEEEEEECCCCCCCC
DIPGIIRKTILDIGYTSEDAGIDGNTCSIQESITSQSKEIADAVNFSLEYRNQQGDLGRN
CCCHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCC
SFSQQGSGDQGSVFGYACRETPEMMPLPITIAHKLAYSLAFVRKEKILPYLLPDGKSQVT
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEE
LGYDSANRPKTLETVVISAQHEDSVDLDKLRFDILERVVRPVISATGLDCSKATFLINPA
EECCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GRFVTGGPSADSGLTGRKIVVDTYGCAAKHGGGALSGKDPSKLDRFASYMARWVAKHVVA
CCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ADFAESIEVQISYAIGKAHPVAFNIDTHGTSTIALDKLKCAILKVFDFRPAAVIDSLDLK
HHHHHCEEEEEEEEECCCCCEEEEECCCCCCEEEHHHHHEEHHHHHCCCCHHHHHCHHCC
RPIYQKTAAYGHFGRDIFTWERICPDKLNALLGAV
CHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MILTSESVTEGHPDKLCDQISDAILDGVICKDKNARAGIETIAGNGVVHVFGEVSNPDSV
CEECCCCCCCCCHHHHHHHHHHHHHHCEEECCCCCCCCHHHHCCCCEEEEEECCCCCCCC
DIPGIIRKTILDIGYTSEDAGIDGNTCSIQESITSQSKEIADAVNFSLEYRNQQGDLGRN
CCCHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCC
SFSQQGSGDQGSVFGYACRETPEMMPLPITIAHKLAYSLAFVRKEKILPYLLPDGKSQVT
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEE
LGYDSANRPKTLETVVISAQHEDSVDLDKLRFDILERVVRPVISATGLDCSKATFLINPA
EECCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GRFVTGGPSADSGLTGRKIVVDTYGCAAKHGGGALSGKDPSKLDRFASYMARWVAKHVVA
CCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ADFAESIEVQISYAIGKAHPVAFNIDTHGTSTIALDKLKCAILKVFDFRPAAVIDSLDLK
HHHHHCEEEEEEEEECCCCCEEEEECCCCCCEEEHHHHHEEHHHHHCCCCHHHHHCHHCC
RPIYQKTAAYGHFGRDIFTWERICPDKLNALLGAV
CHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174