Definition Tropheryma whipplei TW08/27, complete genome.
Accession NC_004551
Length 925,938

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The map label for this gene is leuS

Identifier: 28572538

GI number: 28572538

Start: 418111

End: 420531

Strand: Direct

Name: leuS

Synonym: TW385

Alternate gene names: 28572538

Gene position: 418111-420531 (Clockwise)

Preceding gene: 28572537

Following gene: 28572539

Centisome position: 45.16

GC content: 43.95

Gene sequence:

>2421_bases
ATGCATGCGTTACGTGCCTTAGATACATTGTGTGAGAATATGGAATACAATTTTCGTGCCTTAGAAGAAAAATGGGCCCC
AATCTGGGAGCGCGATAGGTTATTTGAGGTTGATGAAAATGATTCTGAAACACCTCGAAAATACGTTTTGGATATGTTTT
CCTATCCCTCAGGGGACTTACATATGGGACATGCTGAAACATATGCTTACGGTGATTTCATAGCTCGCTACTGGCGTCAC
AGGGGCTACAATGTACTTCATCCTGTTGGGTGGGACTCTTTTGGCTTGCCTGCGGAAAATGCCGCAATAAAGCACGGTTC
AGATCCAAAAGTCTGGACTTATAGGAATATTGATCAGCAAGCAAGAAGTATGCGACTTTATGCCGCGTCGTTTGACTGGT
CTCGACGGTTGCATACATCAGACCCTGAGTACTACAGATGGAATCAATGGCTTTTTCTAAAACTTTATAAACACGGTCTT
GCCTACAGGAAAAAGGCATGGGTTAATTGGGATCCTTCTGACAGAACTGTTCTTGCTAATGAGCAGGTTCTACCGGATGG
CACTAGCGAGCGCAGCGGAGCTTTAGTTGTCAAAAAGAAGCTTACTCAGTGGTTTTTGCGAATAACTGCGTATGCTGATC
GTCTTCTTGATGACTTGAGCATGCTTGAAAATAATTGGCCAGAGCGAGTGATTACAATGCAGCGCAACTGGATCGGCCGC
TCGGAGGGTGTTTCGATTGAATTCAATATTCCAACACTAAAGCGACCTGTAATGGTGTTTACAACTCGTCCTGAAACTAT
TTTTGGTGTCACTTATCTTGCGCTTGCTTTTGATTCTGAGGTGACAGAAGAGCTAGCGAGCAAATCTGGCGTACTAGGCG
AGTTGCTCGAATTGCGACATAATATTGATAAAACCCATGAGAGTGTCCGTGGTCTAGACCTAAAATCCTTTGCAATTCAC
CCGCTTACAGGACAGAGTATCCCAATTTTTGCGGCGAGCTACATTCTTTCTGACTACGCAAAGGGGGCTGTTATGTCGGT
TCCAGGTCATGACACTCGCGATGAGAGGTTTGCTGTGAGGTACAACCTTCCAATAGTCAAAATTATGGAAGATAATCGCC
TTATTTCTTCTGGTAAATACTCTGGGCAGAGTGTCACGCAAGCAAGGGAAAATATTACTAGAGATCTATGCGCGAAGTCC
CTTGGAAGAAGAGAAATATCCTATAGGCTCCGTGACTGGCTTATTTCAAGACAGAGATATTGGGGGACCCCAATACCGAT
TCTGTACGATTCAAATGGCTCCGAAATTCCTGTTGAAGAAGATGATTTACCTGTTCTATTGCCGGATTCAGAGGGTATTG
ATCTAACTCCAAGCGGACTGTCACCACTTGGTGGTATTCATGACTGGGTAAACCTTCATAAGGCTGGTTCGCTCTTTCGA
AGAGATACAGATACGATGGACACATTTTTTGATTCATCGTGGTACTTTCTGCGATATCTGAATCCTGATTGTGATACTGC
GCCGTTTACCTTGGAGAAGGCTAAGAAATGGGGCCCTGTAGATCAGTACTGTGGCGGGGTTGAACATGCCGTGCTGCATT
TGTTATACGCAAGGTTTATAACCAAATTCTTGTACGATATCGGGTTTGTCGATTTTAAAGAACCATTTCTGCGTCTTATA
AATCAGGGCATGGTTGTCCTGAATGGGGCAAAAATGTCAAAGTCAAAGGGCAATATTGTTGAATTTTCGAAGGAAGTTTC
CCAACACGGAGTTGATGTAATCCGGTTCGCTCTAATATTTTCTGGACCACCAGAAGAAGATATTGATTGGAAAGATGTAT
CAATGACAGGAGCGGCAAGGTTTCTATCGCGTTGCATTCAAACTGCAAAAGAAGTTCCAAAGCGCACCGCCGATCTTTCA
TTGGGGGATATTGAGCTCAGGAAACACACACACAGCTTATTGAATGATATCGACTGGTTAGTCGATTCTTATCGTTTTAA
TGTTATTGCCGCACGGCTAATGGATCTTCTGAATATAACTAGAAAAAAAATTCAGACAATAGGGGCTGATAATCCTGCTA
TACGAGAGGCAATTGAAACAATTGCGATTGCCCTTGATATGTTTTCCCCCTACACAGCCGAGGAAATGTGGGAAATTTTG
GGCAATAAATACAGCGTCAGTAAAGCGCTTTTCCCAGAAGTTGATACAACTTTCTTAGAACAGAAAACCACCTGTGCAAT
AGTGCAGATAGACGGTAGACTACGAGGTCGACTGAACGTTTTGACTAATATAACGACCGAGCAGCTTGTACACAGCGCCC
GGAGCCTGCCGGCAATTGAGCATGCGCTTTCTGGCAGGTCAGTAAAGCGGGTAATCTGTGTTCCACCAAAACTTGTAAAC
TTCGTTGTGGAGCCCAAATAG

Upstream 100 bases:

>100_bases
GAACTTGCCGAGACTAAACTGAAGGTAGCGGCAATGATTGATGCGATTTTGGGTAGGTAGATACTTTTCATGAAAACCTA
TAACCGGTTCATCCAAGAAC

Downstream 100 bases:

>100_bases
ATCGTGCTGCAAGCTAGGTAAGTTTTACACAGTTTTTGCTTTTTCATTTGTTCATAGGGTGTAGCAAAATATCTTTTCTC
TATGCACAAGTTTCGCTGGA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 806; Mature: 806

Protein sequence:

>806_residues
MHALRALDTLCENMEYNFRALEEKWAPIWERDRLFEVDENDSETPRKYVLDMFSYPSGDLHMGHAETYAYGDFIARYWRH
RGYNVLHPVGWDSFGLPAENAAIKHGSDPKVWTYRNIDQQARSMRLYAASFDWSRRLHTSDPEYYRWNQWLFLKLYKHGL
AYRKKAWVNWDPSDRTVLANEQVLPDGTSERSGALVVKKKLTQWFLRITAYADRLLDDLSMLENNWPERVITMQRNWIGR
SEGVSIEFNIPTLKRPVMVFTTRPETIFGVTYLALAFDSEVTEELASKSGVLGELLELRHNIDKTHESVRGLDLKSFAIH
PLTGQSIPIFAASYILSDYAKGAVMSVPGHDTRDERFAVRYNLPIVKIMEDNRLISSGKYSGQSVTQARENITRDLCAKS
LGRREISYRLRDWLISRQRYWGTPIPILYDSNGSEIPVEEDDLPVLLPDSEGIDLTPSGLSPLGGIHDWVNLHKAGSLFR
RDTDTMDTFFDSSWYFLRYLNPDCDTAPFTLEKAKKWGPVDQYCGGVEHAVLHLLYARFITKFLYDIGFVDFKEPFLRLI
NQGMVVLNGAKMSKSKGNIVEFSKEVSQHGVDVIRFALIFSGPPEEDIDWKDVSMTGAARFLSRCIQTAKEVPKRTADLS
LGDIELRKHTHSLLNDIDWLVDSYRFNVIAARLMDLLNITRKKIQTIGADNPAIREAIETIAIALDMFSPYTAEEMWEIL
GNKYSVSKALFPEVDTTFLEQKTTCAIVQIDGRLRGRLNVLTNITTEQLVHSARSLPAIEHALSGRSVKRVICVPPKLVN
FVVEPK

Sequences:

>Translated_806_residues
MHALRALDTLCENMEYNFRALEEKWAPIWERDRLFEVDENDSETPRKYVLDMFSYPSGDLHMGHAETYAYGDFIARYWRH
RGYNVLHPVGWDSFGLPAENAAIKHGSDPKVWTYRNIDQQARSMRLYAASFDWSRRLHTSDPEYYRWNQWLFLKLYKHGL
AYRKKAWVNWDPSDRTVLANEQVLPDGTSERSGALVVKKKLTQWFLRITAYADRLLDDLSMLENNWPERVITMQRNWIGR
SEGVSIEFNIPTLKRPVMVFTTRPETIFGVTYLALAFDSEVTEELASKSGVLGELLELRHNIDKTHESVRGLDLKSFAIH
PLTGQSIPIFAASYILSDYAKGAVMSVPGHDTRDERFAVRYNLPIVKIMEDNRLISSGKYSGQSVTQARENITRDLCAKS
LGRREISYRLRDWLISRQRYWGTPIPILYDSNGSEIPVEEDDLPVLLPDSEGIDLTPSGLSPLGGIHDWVNLHKAGSLFR
RDTDTMDTFFDSSWYFLRYLNPDCDTAPFTLEKAKKWGPVDQYCGGVEHAVLHLLYARFITKFLYDIGFVDFKEPFLRLI
NQGMVVLNGAKMSKSKGNIVEFSKEVSQHGVDVIRFALIFSGPPEEDIDWKDVSMTGAARFLSRCIQTAKEVPKRTADLS
LGDIELRKHTHSLLNDIDWLVDSYRFNVIAARLMDLLNITRKKIQTIGADNPAIREAIETIAIALDMFSPYTAEEMWEIL
GNKYSVSKALFPEVDTTFLEQKTTCAIVQIDGRLRGRLNVLTNITTEQLVHSARSLPAIEHALSGRSVKRVICVPPKLVN
FVVEPK
>Mature_806_residues
MHALRALDTLCENMEYNFRALEEKWAPIWERDRLFEVDENDSETPRKYVLDMFSYPSGDLHMGHAETYAYGDFIARYWRH
RGYNVLHPVGWDSFGLPAENAAIKHGSDPKVWTYRNIDQQARSMRLYAASFDWSRRLHTSDPEYYRWNQWLFLKLYKHGL
AYRKKAWVNWDPSDRTVLANEQVLPDGTSERSGALVVKKKLTQWFLRITAYADRLLDDLSMLENNWPERVITMQRNWIGR
SEGVSIEFNIPTLKRPVMVFTTRPETIFGVTYLALAFDSEVTEELASKSGVLGELLELRHNIDKTHESVRGLDLKSFAIH
PLTGQSIPIFAASYILSDYAKGAVMSVPGHDTRDERFAVRYNLPIVKIMEDNRLISSGKYSGQSVTQARENITRDLCAKS
LGRREISYRLRDWLISRQRYWGTPIPILYDSNGSEIPVEEDDLPVLLPDSEGIDLTPSGLSPLGGIHDWVNLHKAGSLFR
RDTDTMDTFFDSSWYFLRYLNPDCDTAPFTLEKAKKWGPVDQYCGGVEHAVLHLLYARFITKFLYDIGFVDFKEPFLRLI
NQGMVVLNGAKMSKSKGNIVEFSKEVSQHGVDVIRFALIFSGPPEEDIDWKDVSMTGAARFLSRCIQTAKEVPKRTADLS
LGDIELRKHTHSLLNDIDWLVDSYRFNVIAARLMDLLNITRKKIQTIGADNPAIREAIETIAIALDMFSPYTAEEMWEIL
GNKYSVSKALFPEVDTTFLEQKTTCAIVQIDGRLRGRLNVLTNITTEQLVHSARSLPAIEHALSGRSVKRVICVPPKLVN
FVVEPK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=861, Percent_Identity=33.5656213704994, Blast_Score=438, Evalue=1e-122,
Organism=Escherichia coli, GI1786861, Length=863, Percent_Identity=37.5434530706837, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1790708, Length=373, Percent_Identity=25.201072386059, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71997510, Length=811, Percent_Identity=31.5659679408138, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71997517, Length=771, Percent_Identity=32.0363164721141, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI212645227, Length=300, Percent_Identity=31.3333333333333, Blast_Score=132, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17510661, Length=410, Percent_Identity=25.1219512195122, Blast_Score=101, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323414, Length=842, Percent_Identity=36.4608076009501, Blast_Score=514, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6321531, Length=416, Percent_Identity=24.2788461538462, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6325217, Length=376, Percent_Identity=22.8723404255319, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21355409, Length=723, Percent_Identity=34.716459197787, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17864482, Length=656, Percent_Identity=21.7987804878049, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24653289, Length=656, Percent_Identity=21.7987804878049, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_TROW8 (Q83HV5)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789318.1
- ProteinModelPortal:   Q83HV5
- SMR:   Q83HV5
- STRING:   Q83HV5
- GeneID:   1064671
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW385
- eggNOG:   COG0495
- HOGENOM:   HBG693845
- OMA:   ERGNFPV
- PhylomeDB:   Q83HV5
- ProtClustDB:   PRK00390
- BioCyc:   TWHI218496:TW0363-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 92375; Mature: 92375

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 574-574

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MHALRALDTLCENMEYNFRALEEKWAPIWERDRLFEVDENDSETPRKYVLDMFSYPSGDL
CCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCE
HMGHAETYAYGDFIARYWRHRGYNVLHPVGWDSFGLPAENAAIKHGSDPKVWTYRNIDQQ
ECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHH
ARSMRLYAASFDWSRRLHTSDPEYYRWNQWLFLKLYKHGLAYRKKAWVNWDPSDRTVLAN
HHHEEEEEECCCHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEC
EQVLPDGTSERSGALVVKKKLTQWFLRITAYADRLLDDLSMLENNWPERVITMQRNWIGR
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHCCCC
SEGVSIEFNIPTLKRPVMVFTTRPETIFGVTYLALAFDSEVTEELASKSGVLGELLELRH
CCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHH
NIDKTHESVRGLDLKSFAIHPLTGQSIPIFAASYILSDYAKGAVMSVPGHDTRDERFAVR
HHHHHHHHHCCCCCHHEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEE
YNLPIVKIMEDNRLISSGKYSGQSVTQARENITRDLCAKSLGRREISYRLRDWLISRQRY
ECCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGTPIPILYDSNGSEIPVEEDDLPVLLPDSEGIDLTPSGLSPLGGIHDWVNLHKAGSLFR
CCCCCCEEECCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHH
RDTDTMDTFFDSSWYFLRYLNPDCDTAPFTLEKAKKWGPVDQYCGGVEHAVLHLLYARFI
CCCHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHCCCCHHHHHCCHHHHHHHHHHHHHH
TKFLYDIGFVDFKEPFLRLINQGMVVLNGAKMSKSKGNIVEFSKEVSQHGVDVIRFALIF
HHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHC
SGPPEEDIDWKDVSMTGAARFLSRCIQTAKEVPKRTADLSLGDIELRKHTHSLLNDIDWL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
VDSYRFNVIAARLMDLLNITRKKIQTIGADNPAIREAIETIAIALDMFSPYTAEEMWEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GNKYSVSKALFPEVDTTFLEQKTTCAIVQIDGRLRGRLNVLTNITTEQLVHSARSLPAIE
CCCEECHHHHCCCCCHHHHCCCCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHCCHHHH
HALSGRSVKRVICVPPKLVNFVVEPK
HHHCCCCCEEEEECCHHHHCEEECCC
>Mature Secondary Structure
MHALRALDTLCENMEYNFRALEEKWAPIWERDRLFEVDENDSETPRKYVLDMFSYPSGDL
CCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCE
HMGHAETYAYGDFIARYWRHRGYNVLHPVGWDSFGLPAENAAIKHGSDPKVWTYRNIDQQ
ECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHH
ARSMRLYAASFDWSRRLHTSDPEYYRWNQWLFLKLYKHGLAYRKKAWVNWDPSDRTVLAN
HHHEEEEEECCCHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEC
EQVLPDGTSERSGALVVKKKLTQWFLRITAYADRLLDDLSMLENNWPERVITMQRNWIGR
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHCCCC
SEGVSIEFNIPTLKRPVMVFTTRPETIFGVTYLALAFDSEVTEELASKSGVLGELLELRH
CCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHH
NIDKTHESVRGLDLKSFAIHPLTGQSIPIFAASYILSDYAKGAVMSVPGHDTRDERFAVR
HHHHHHHHHCCCCCHHEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEE
YNLPIVKIMEDNRLISSGKYSGQSVTQARENITRDLCAKSLGRREISYRLRDWLISRQRY
ECCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGTPIPILYDSNGSEIPVEEDDLPVLLPDSEGIDLTPSGLSPLGGIHDWVNLHKAGSLFR
CCCCCCEEECCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHH
RDTDTMDTFFDSSWYFLRYLNPDCDTAPFTLEKAKKWGPVDQYCGGVEHAVLHLLYARFI
CCCHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHCCCCHHHHHCCHHHHHHHHHHHHHH
TKFLYDIGFVDFKEPFLRLINQGMVVLNGAKMSKSKGNIVEFSKEVSQHGVDVIRFALIF
HHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHC
SGPPEEDIDWKDVSMTGAARFLSRCIQTAKEVPKRTADLSLGDIELRKHTHSLLNDIDWL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
VDSYRFNVIAARLMDLLNITRKKIQTIGADNPAIREAIETIAIALDMFSPYTAEEMWEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GNKYSVSKALFPEVDTTFLEQKTTCAIVQIDGRLRGRLNVLTNITTEQLVHSARSLPAIE
CCCEECHHHHCCCCCHHHHCCCCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHCCHHHH
HALSGRSVKRVICVPPKLVNFVVEPK
HHHCCCCCEEEEECCHHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174