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The map label for this gene is nei [C]

Identifier: 28572527

GI number: 28572527

Start: 406503

End: 407387

Strand: Direct

Name: nei [C]

Synonym: TW374

Alternate gene names: 28572527

Gene position: 406503-407387 (Clockwise)

Preceding gene: 28572526

Following gene: 28572537

Centisome position: 43.9

GC content: 48.93

Gene sequence:

>885_bases
ATGCCTGAGGGGCATTCAATCCACCGTTCTGCAATTCAATTTGAGCATAATTTCGTTGGAAAAAAAATAGGGATTTCTTC
CCCGCAGGGGAGGTTTTCCCCTGGTGCGCGTCTACTTGACAAGCAAAAAATGATATCTGCCAAAGCATACGGAAAGCAGC
TGCTTCTGGGCTTTGAAAATAACCGGTTCTTGCAAGTTCATCTGGGGATATACGGCGCTTGGCAATTCTATGGGAATATA
TCCTGCGCACCAACTATATTGCCCGGGGCAGAAGGTATTCAAAGCATTGGTGCCCCCAGGCGATTGAAGGGGCCTTGCAT
AACCAAGAACAGCGGCTCTACCCCCGGGGCATCTTGGAAAGACCTGATGTTTAAGCATCAGGCCCAGGCAAGAGCTAAGC
TCATTACAGACACCGCAGGGGTTCTTCTGAGCGGACCGGCGGTCTGCAAAGTTTTGACACCCGAAGAGGCGGATGAGCTT
ATCTCGCGACTTGGTCCGGATCCGGTCGTCAATCCGATGGGATACAACCGCTTTATCAAAGCAGCTCGAAAAAGCAACCT
CCAAATATGCAAGCTACTCATGAACCAGTCTGCTATCGCTGGTATAGGGAATATATACCGCGCGGAAATTCTATTCAGAA
ATAGAGTAAACCCATTTCTTACCTCGAACACCATCCCGGAAGACACCTTAAAAAGGATATGGGACGACTGGACAGTCCTG
TTAAAATGCGGAATAAAAACCGGACAGATGATCACTATGAATAAGGCGCCGGGACACTGGGTTTACAATCACCACAAGTC
CCCATGTTCAGTCTGCTTTCACCCAATAGATTGCGTAAACGTGGCAGGCAGAAAGCTGTACTGGTGCGTCGTCTGTCAAG
CGTAG

Upstream 100 bases:

>100_bases
GGAACCATTCAGTGAGGACTCGCGGCATATCAGAAGGATAGATGCGATAAGTTCGTATGAGTGTCAGTCTTGATTGTACC
TTTGATTTGTTCTCTGACGA

Downstream 100 bases:

>100_bases
TTTACTCAACCGTCTTATGCGCCAAAAGAAACCAACGGTCTTTATCCAGGGTTCTTGCAACTTCTATGACAGCATCTTGA
CTCGCGGCATCAAGTAAGCT

Product: endonuclease VIII

Products: NA

Alternate protein names: Putative DNA-(apurinic or apyrimidinic site) lyase Rv2464c/MT2539; Putative AP lyase Rv2464c/MT2539 [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MPEGHSIHRSAIQFEHNFVGKKIGISSPQGRFSPGARLLDKQKMISAKAYGKQLLLGFENNRFLQVHLGIYGAWQFYGNI
SCAPTILPGAEGIQSIGAPRRLKGPCITKNSGSTPGASWKDLMFKHQAQARAKLITDTAGVLLSGPAVCKVLTPEEADEL
ISRLGPDPVVNPMGYNRFIKAARKSNLQICKLLMNQSAIAGIGNIYRAEILFRNRVNPFLTSNTIPEDTLKRIWDDWTVL
LKCGIKTGQMITMNKAPGHWVYNHHKSPCSVCFHPIDCVNVAGRKLYWCVVCQA

Sequences:

>Translated_294_residues
MPEGHSIHRSAIQFEHNFVGKKIGISSPQGRFSPGARLLDKQKMISAKAYGKQLLLGFENNRFLQVHLGIYGAWQFYGNI
SCAPTILPGAEGIQSIGAPRRLKGPCITKNSGSTPGASWKDLMFKHQAQARAKLITDTAGVLLSGPAVCKVLTPEEADEL
ISRLGPDPVVNPMGYNRFIKAARKSNLQICKLLMNQSAIAGIGNIYRAEILFRNRVNPFLTSNTIPEDTLKRIWDDWTVL
LKCGIKTGQMITMNKAPGHWVYNHHKSPCSVCFHPIDCVNVAGRKLYWCVVCQA
>Mature_293_residues
PEGHSIHRSAIQFEHNFVGKKIGISSPQGRFSPGARLLDKQKMISAKAYGKQLLLGFENNRFLQVHLGIYGAWQFYGNIS
CAPTILPGAEGIQSIGAPRRLKGPCITKNSGSTPGASWKDLMFKHQAQARAKLITDTAGVLLSGPAVCKVLTPEEADELI
SRLGPDPVVNPMGYNRFIKAARKSNLQICKLLMNQSAIAGIGNIYRAEILFRNRVNPFLTSNTIPEDTLKRIWDDWTVLL
KCGIKTGQMITMNKAPGHWVYNHHKSPCSVCFHPIDCVNVAGRKLYWCVVCQA

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA b

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 32483; Mature: 32352

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS51066 ZF_FPG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGHSIHRSAIQFEHNFVGKKIGISSPQGRFSPGARLLDKQKMISAKAYGKQLLLGFEN
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECC
NRFLQVHLGIYGAWQFYGNISCAPTILPGAEGIQSIGAPRRLKGPCITKNSGSTPGASWK
CCEEEEEEEEEEEEEECCCCCCCCEECCCHHHHHHCCCCCCCCCCEEECCCCCCCCCCHH
DLMFKHQAQARAKLITDTAGVLLSGPAVCKVLTPEEADELISRLGPDPVVNPMGYNRFIK
HHHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHH
AARKSNLQICKLLMNQSAIAGIGNIYRAEILFRNRVNPFLTSNTIPEDTLKRIWDDWTVL
HHHHCCHHHHHHHHCCHHHHHHCHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHEE
LKCGIKTGQMITMNKAPGHWVYNHHKSPCSVCFHPIDCVNVAGRKLYWCVVCQA
HHCCCCCCCEEEECCCCCCEEECCCCCHHHHEECCHHHHHHCCCEEEEEEEECC
>Mature Secondary Structure 
PEGHSIHRSAIQFEHNFVGKKIGISSPQGRFSPGARLLDKQKMISAKAYGKQLLLGFEN
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECC
NRFLQVHLGIYGAWQFYGNISCAPTILPGAEGIQSIGAPRRLKGPCITKNSGSTPGASWK
CCEEEEEEEEEEEEEECCCCCCCCEECCCHHHHHHCCCCCCCCCCEEECCCCCCCCCCHH
DLMFKHQAQARAKLITDTAGVLLSGPAVCKVLTPEEADELISRLGPDPVVNPMGYNRFIK
HHHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHH
AARKSNLQICKLLMNQSAIAGIGNIYRAEILFRNRVNPFLTSNTIPEDTLKRIWDDWTVL
HHHHCCHHHHHHHHCCHHHHHHCHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHEE
LKCGIKTGQMITMNKAPGHWVYNHHKSPCSVCFHPIDCVNVAGRKLYWCVVCQA
HHCCCCCCCEEEECCCCCCEEECCCCCHHHHEECCHHHHHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]