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The map label for this gene is nadE

Identifier: 28572518

GI number: 28572518

Start: 397571

End: 398386

Strand: Direct

Name: nadE

Synonym: TW365

Alternate gene names: 28572518

Gene position: 397571-398386 (Clockwise)

Preceding gene: 28572517

Following gene: 28572520

Centisome position: 42.94

GC content: 46.94

Gene sequence:

>816_bases
GTGTGTTGTGATATATCAAAGACTCTATGCGTGAAGCCGTTTATAGATCCTGAAGAAGAGATTTCACACAGGGTGTCGTT
TCTGGCCGATTATTTGCGCCATTCCCGCGCATCGGGTTATGTTCTTGGTATAAGCGGTGGACAGGATTCCGCCCTGGCCG
GTAGATTGTGCCAGATCGCCGTTGAAAGTGTGCGATCTATTGGTTTTGATGCAACCCTCTGGGCCATCCGCCTACCCTAC
GGGCAACAGTTTGATGAGTCTGATGCCCAAACTGCAATGCAATTTATATCTCCCGATGAGGAGCTTAGTTTCGACATACG
TAGCGCAACAGACAATCTATGCGTGGATCTCAATAGGTCTCTTGGGTCTAAGATAAGCGATTTTAATCGCGGCAATATAA
AAGCAAGGTTGCGCATGGTTGTGCAGTACGCGGTTGCCGCCCATCATGATGCTCTTGTTGTTGGAACAGATCATGCGGCT
GAGGCAGTAACCGGGTTTTTTACAAAATTTGGTGATGGCGCAGCAGATATTTTGCCTCTATACGGTCTTACAAAGGGTCA
AGGCAGGGCTCTCCTCAAAGCCCTTGGCGCATGCGACTCAATTATCGAAAAAGTTCCAACAGCCGATTTATTAGACGATT
TACCGTGTTTGCCTGATGAAACTGAGTTAGGGTTACAGTACCGAGATATAGATGCCTTTTTGGAAGGAAAACCGGTTTCT
GAAGATATTACGATGGCTATTACTGAGCGGTACAAATCGACCCTTCATAAAAGAATGCCCCCCATAACCCCACACTCAAC
ATGGTGGCGCAAATAA

Upstream 100 bases:

>100_bases
TTTATCTTGGTGGATATAGCTCAGCTGGTAGAGCACCTGGTTGTGGTCCAGGATGTCGCGGGTTCGAGTCCCGTTATCCA
CCCTGAGTTCTGGGAGCTTT

Downstream 100 bases:

>100_bases
TCTCCTGGTAAATGATCATGCTATGAGTTGCGCACGAGGGGCCACACAGGTTTATTTGCCCCCTGCCTGAATGTCCTTTA
CCTGCCTTAGGATATCCCTC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MCCDISKTLCVKPFIDPEEEISHRVSFLADYLRHSRASGYVLGISGGQDSALAGRLCQIAVESVRSIGFDATLWAIRLPY
GQQFDESDAQTAMQFISPDEELSFDIRSATDNLCVDLNRSLGSKISDFNRGNIKARLRMVVQYAVAAHHDALVVGTDHAA
EAVTGFFTKFGDGAADILPLYGLTKGQGRALLKALGACDSIIEKVPTADLLDDLPCLPDETELGLQYRDIDAFLEGKPVS
EDITMAITERYKSTLHKRMPPITPHSTWWRK

Sequences:

>Translated_271_residues
MCCDISKTLCVKPFIDPEEEISHRVSFLADYLRHSRASGYVLGISGGQDSALAGRLCQIAVESVRSIGFDATLWAIRLPY
GQQFDESDAQTAMQFISPDEELSFDIRSATDNLCVDLNRSLGSKISDFNRGNIKARLRMVVQYAVAAHHDALVVGTDHAA
EAVTGFFTKFGDGAADILPLYGLTKGQGRALLKALGACDSIIEKVPTADLLDDLPCLPDETELGLQYRDIDAFLEGKPVS
EDITMAITERYKSTLHKRMPPITPHSTWWRK
>Mature_271_residues
MCCDISKTLCVKPFIDPEEEISHRVSFLADYLRHSRASGYVLGISGGQDSALAGRLCQIAVESVRSIGFDATLWAIRLPY
GQQFDESDAQTAMQFISPDEELSFDIRSATDNLCVDLNRSLGSKISDFNRGNIKARLRMVVQYAVAAHHDALVVGTDHAA
EAVTGFFTKFGDGAADILPLYGLTKGQGRALLKALGACDSIIEKVPTADLLDDLPCLPDETELGLQYRDIDAFLEGKPVS
EDITMAITERYKSTLHKRMPPITPHSTWWRK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=270, Percent_Identity=50.7407407407407, Blast_Score=250, Evalue=7e-68,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_TROW8 (Q83HW8)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789298.1
- ProteinModelPortal:   Q83HW8
- SMR:   Q83HW8
- STRING:   Q83HW8
- GeneID:   1064651
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW365
- eggNOG:   COG0171
- HOGENOM:   HBG351567
- OMA:   GLNKRRV
- PhylomeDB:   Q83HW8
- ProtClustDB:   PRK00768
- BioCyc:   TWHI218496:TW0343-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 29841; Mature: 29841

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: ACT_SITE 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCCDISKTLCVKPFIDPEEEISHRVSFLADYLRHSRASGYVLGISGGQDSALAGRLCQIA
CCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
VESVRSIGFDATLWAIRLPYGQQFDESDAQTAMQFISPDEELSFDIRSATDNLCVDLNRS
HHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHCEEHHHCCCCCEEECCHH
LGSKISDFNRGNIKARLRMVVQYAVAAHHDALVVGTDHAAEAVTGFFTKFGDGAADILPL
HCCHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHH
YGLTKGQGRALLKALGACDSIIEKVPTADLLDDLPCLPDETELGLQYRDIDAFLEGKPVS
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHCCCEEECHHHHHCCCCCC
EDITMAITERYKSTLHKRMPPITPHSTWWRK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MCCDISKTLCVKPFIDPEEEISHRVSFLADYLRHSRASGYVLGISGGQDSALAGRLCQIA
CCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
VESVRSIGFDATLWAIRLPYGQQFDESDAQTAMQFISPDEELSFDIRSATDNLCVDLNRS
HHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHCEEHHHCCCCCEEECCHH
LGSKISDFNRGNIKARLRMVVQYAVAAHHDALVVGTDHAAEAVTGFFTKFGDGAADILPL
HCCHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHH
YGLTKGQGRALLKALGACDSIIEKVPTADLLDDLPCLPDETELGLQYRDIDAFLEGKPVS
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHCCCEEECHHHHHCCCCCC
EDITMAITERYKSTLHKRMPPITPHSTWWRK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174