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The map label for this gene is trmD

Identifier: 28572463

GI number: 28572463

Start: 342884

End: 343588

Strand: Direct

Name: trmD

Synonym: TW307

Alternate gene names: 28572463

Gene position: 342884-343588 (Clockwise)

Preceding gene: 28572462

Following gene: 28572464

Centisome position: 37.03

GC content: 42.55

Gene sequence:

>705_bases
TTGTTTGTCATGCGTTGTGATTTTGTAAGTATATTTCCTGAATATTTTGATGTCTTAGATATATCTCTAATTGGGAAAGC
TAGACGAAATGGACTGCTGGATTTAAGAGTCCATAACCTCAGGGAATATAGTGAAGCCGGTAGGGTTGATAGCTCGCCAT
ATGGGGGCGGACCCGGTATGGTAATGAGTGCAGAGCCTTGGGCACGTGCAATAGAACACATTGCTACCGGAGAATCTCTA
GTTGTTTTTCCATCACCCTCAGGACAACCATATTCCCACGATTTGGCCCAATCTCTATCGTCCGAGATGCATATAGTTTT
TTGTTGCGGGAGATATGAGGGGATTGATAATAGGATTTACGAATGGACCGCCACTCGTTTACGGTCTTCTGGAATAAGTA
TTGGGGATTATGTACTAAATGGCGGTGAGATTGCCGCGCTGGTTATTCTTGAAGGTTTTGTTCGATTTATACCGGGTGTG
TTGGGTAACCCAGAAAGCCTGGTAGAGGAGAGCTATCAGTATAATCTACTTGAATATCCGGTTTATACCAAGCCTGCGGT
CTGGAGAGGTCTAGAGGTTCCCGACATTCTTTTGTCTGGCAATCATGATTTGATAAGGGAATGGAGATATAAAAAACAAT
TGGAAATAACTCAAAAGACCAGACCTGATTTGTACTCGACGCACATATATGAAACTGATTCTTAA

Upstream 100 bases:

>100_bases
CAGATGGCGAGATAATGGTAAACCCACCTGGTGGAATGTTCGTTTCACAAGTAGATTCTGCCTCCTAATAGACACTTGTT
GCTGTGTCGGCCTATTATCT

Downstream 100 bases:

>100_bases
ACTTTGACACCCGCGCGACAGGTTATTTGATGGAGAGTAAAAAAGCCAGTTTTCTGTGGTAGCATTCCAGGGTTCGTTCT
TTTAGGTTTGTTGTGCATGC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MFVMRCDFVSIFPEYFDVLDISLIGKARRNGLLDLRVHNLREYSEAGRVDSSPYGGGPGMVMSAEPWARAIEHIATGESL
VVFPSPSGQPYSHDLAQSLSSEMHIVFCCGRYEGIDNRIYEWTATRLRSSGISIGDYVLNGGEIAALVILEGFVRFIPGV
LGNPESLVEESYQYNLLEYPVYTKPAVWRGLEVPDILLSGNHDLIREWRYKKQLEITQKTRPDLYSTHIYETDS

Sequences:

>Translated_234_residues
MFVMRCDFVSIFPEYFDVLDISLIGKARRNGLLDLRVHNLREYSEAGRVDSSPYGGGPGMVMSAEPWARAIEHIATGESL
VVFPSPSGQPYSHDLAQSLSSEMHIVFCCGRYEGIDNRIYEWTATRLRSSGISIGDYVLNGGEIAALVILEGFVRFIPGV
LGNPESLVEESYQYNLLEYPVYTKPAVWRGLEVPDILLSGNHDLIREWRYKKQLEITQKTRPDLYSTHIYETDS
>Mature_234_residues
MFVMRCDFVSIFPEYFDVLDISLIGKARRNGLLDLRVHNLREYSEAGRVDSSPYGGGPGMVMSAEPWARAIEHIATGESL
VVFPSPSGQPYSHDLAQSLSSEMHIVFCCGRYEGIDNRIYEWTATRLRSSGISIGDYVLNGGEIAALVILEGFVRFIPGV
LGNPESLVEESYQYNLLEYPVYTKPAVWRGLEVPDILLSGNHDLIREWRYKKQLEITQKTRPDLYSTHIYETDS

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=233, Percent_Identity=39.9141630901288, Blast_Score=159, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_TROW8 (Q820Z0)

Other databases:

- EMBL:   BX251411
- RefSeq:   NP_789243.1
- ProteinModelPortal:   Q820Z0
- SMR:   Q820Z0
- STRING:   Q820Z0
- GeneID:   1064596
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW307
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   TWHI218496:TW0288-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26461; Mature: 26461

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVMRCDFVSIFPEYFDVLDISLIGKARRNGLLDLRVHNLREYSEAGRVDSSPYGGGPGM
CEEEECHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCC
VMSAEPWARAIEHIATGESLVVFPSPSGQPYSHDLAQSLSSEMHIVFCCGRYEGIDNRIY
EEECCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHH
EWTATRLRSSGISIGDYVLNGGEIAALVILEGFVRFIPGVLGNPESLVEESYQYNLLEYP
HHHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEECC
VYTKPAVWRGLEVPDILLSGNHDLIREWRYKKQLEITQKTRPDLYSTHIYETDS
CCCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
>Mature Secondary Structure
MFVMRCDFVSIFPEYFDVLDISLIGKARRNGLLDLRVHNLREYSEAGRVDSSPYGGGPGM
CEEEECHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCC
VMSAEPWARAIEHIATGESLVVFPSPSGQPYSHDLAQSLSSEMHIVFCCGRYEGIDNRIY
EEECCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHH
EWTATRLRSSGISIGDYVLNGGEIAALVILEGFVRFIPGVLGNPESLVEESYQYNLLEYP
HHHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEECC
VYTKPAVWRGLEVPDILLSGNHDLIREWRYKKQLEITQKTRPDLYSTHIYETDS
CCCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174