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The map label for this gene is dut

Identifier: 28572433

GI number: 28572433

Start: 312051

End: 312491

Strand: Direct

Name: dut

Synonym: TW277

Alternate gene names: 28572433

Gene position: 312051-312491 (Clockwise)

Preceding gene: 28572431

Following gene: 28572434

Centisome position: 33.7

GC content: 49.89

Gene sequence:

>441_bases
GTGACTGTTGAGGTGTTGTTCAAGGGTGGCTACACGCCGCAGCGCGCTTTTGATGGTGATGCCGGTTTTGATCTTCAGTC
AAGCCACACAGCGGTTATACAACCGCGCTGTCGACAGGTTGTAAAAACAGGTATTGCGATCGCGTTGCCGGATGGCTATG
CCGGTTTTATCATGCCACGCAGCGGGTTAGCTTCTGAGAATGGTATTACACTGGTCAATTCACCGGGCGTGATTGACGCT
GGGTATCGTGGTGAAATATCGGTTGTGCTGATCAATACGGATTTGCACCAGGCTTTTCATATTTCACAGGGTGACCGAAT
TGCTCAGTTGGTTATTATGCCGGTTTGTCATGCAAGTTTTATAGAGGTCGATACTCTTCCTGGGAGTGCAAGGGGCATCT
CTGCTTTTGGTTCAAGCGGAAGGCACGATACACGTGGATGA

Upstream 100 bases:

>100_bases
AAAGCCTTTCTGTATAACGCATAATCATACGAGGTTTATGAACAAGACCGAGAAATATTTTATTTGATATTTATCTGACT
CTTGTGCTAGGTCTATTAGA

Downstream 100 bases:

>100_bases
CAGGTCAAAAACAGGTCCTTTTGACGAATCCGAGGTAGATTCCGTTAGAATCTTCGTTGATCTCGGCGGAATTAAGGTAC
CTCCGTGCGAACGCTTGTCT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MTVEVLFKGGYTPQRAFDGDAGFDLQSSHTAVIQPRCRQVVKTGIAIALPDGYAGFIMPRSGLASENGITLVNSPGVIDA
GYRGEISVVLINTDLHQAFHISQGDRIAQLVIMPVCHASFIEVDTLPGSARGISAFGSSGRHDTRG

Sequences:

>Translated_146_residues
MTVEVLFKGGYTPQRAFDGDAGFDLQSSHTAVIQPRCRQVVKTGIAIALPDGYAGFIMPRSGLASENGITLVNSPGVIDA
GYRGEISVVLINTDLHQAFHISQGDRIAQLVIMPVCHASFIEVDTLPGSARGISAFGSSGRHDTRG
>Mature_145_residues
TVEVLFKGGYTPQRAFDGDAGFDLQSSHTAVIQPRCRQVVKTGIAIALPDGYAGFIMPRSGLASENGITLVNSPGVIDAG
YRGEISVVLINTDLHQAFHISQGDRIAQLVIMPVCHASFIEVDTLPGSARGISAFGSSGRHDTRG

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=130, Percent_Identity=42.3076923076923, Blast_Score=94, Evalue=4e-20,
Organism=Homo sapiens, GI4503423, Length=130, Percent_Identity=42.3076923076923, Blast_Score=94, Evalue=6e-20,
Organism=Homo sapiens, GI70906441, Length=130, Percent_Identity=42.3076923076923, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1790071, Length=133, Percent_Identity=36.8421052631579, Blast_Score=81, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71988561, Length=121, Percent_Identity=42.1487603305785, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319729, Length=129, Percent_Identity=40.3100775193798, Blast_Score=84, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24583610, Length=139, Percent_Identity=37.410071942446, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19921126, Length=139, Percent_Identity=37.410071942446, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_TROW8 (Q83I22)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789213.1
- ProteinModelPortal:   Q83I22
- SMR:   Q83I22
- STRING:   Q83I22
- GeneID:   1064566
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW277
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   GTIDEGY
- PhylomeDB:   Q83I22
- ProtClustDB:   CLSK229619
- BioCyc:   TWHI218496:TW0258-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15441; Mature: 15310

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: BINDING 73-73

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVEVLFKGGYTPQRAFDGDAGFDLQSSHTAVIQPRCRQVVKTGIAIALPDGYAGFIMPR
CEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCEEEEECCCCEEEEEEC
SGLASENGITLVNSPGVIDAGYRGEISVVLINTDLHQAFHISQGDRIAQLVIMPVCHASF
CCCCCCCCEEEEECCCEEECCCCCEEEEEEEECCCCEEEECCCCCCEEEEEEECCCCCCE
IEVDTLPGSARGISAFGSSGRHDTRG
EEEECCCCCCCCEECCCCCCCCCCCC
>Mature Secondary Structure 
TVEVLFKGGYTPQRAFDGDAGFDLQSSHTAVIQPRCRQVVKTGIAIALPDGYAGFIMPR
EEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCEEEEECCCCEEEEEEC
SGLASENGITLVNSPGVIDAGYRGEISVVLINTDLHQAFHISQGDRIAQLVIMPVCHASF
CCCCCCCCEEEEECCCEEECCCCCEEEEEEEECCCCEEEECCCCCCEEEEEEECCCCCCE
IEVDTLPGSARGISAFGSSGRHDTRG
EEEECCCCCCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174