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The map label for this gene is gyrA1 [H]

Identifier: 28572429

GI number: 28572429

Start: 307631

End: 310096

Strand: Reverse

Name: gyrA1 [H]

Synonym: TW273

Alternate gene names: 28572429

Gene position: 310096-307631 (Counterclockwise)

Preceding gene: 28572430

Following gene: 28572426

Centisome position: 33.49

GC content: 43.76

Gene sequence:

>2466_bases
TTGGATAAGGACATCCCTGAGATATTTGATGTAATTTCCGATAACGAGAAAATAACAGATGTAGACCTCTTGCATCAGAT
GCAAGACTCGTTTCTGGAATACGCCTACTCTGTTATTCACTCTCGGGCATTGCCAGACGCGCGGGATGGCTTAAAGCCCG
TACAGAGGCGCATCTTATACACAATGAGTCGGATGGGTCTATCCCCAGATAAAGCCCATGTCAAGTGTGCAAGGGTAACC
GGTGAGGTTATGGGAAAACTTCACCCCCACGGAGATGCAGCAATCTACGACACGCTTGTCAGGATGTCACAGGACTTCAC
AATGCGAATTCCGCTGATTGATGGACACGGCAACTTTGGCTCTCTGAATGATGGGCCGGCAGCGAGCAGATATACAGAGG
CCAGACTATCCAGAGCGGCAGTTGCGATGATTAACGACTTAGAGAGCAATGTTGTTGATTTTGTGCCAAATTATGATAAC
CAATTCACGCAACCTGAAGTTTTACCGGCTCTGTTACCTAACTTGATTGTAAACGGAAGTAGCGGAATAGCCGTCGGAAT
GGCAACCAGTATACCCCCGCATAACCTACAAGAAACCGTTCGAGCAGCTATATATCTTGTTCGCAATAGGCAGTCAAGTC
TAGAGCAAATAATGAAAATCCTGCCAGGACCTGATTTTCCTACAGGTTCGATAATTGTTGGCAAAGAAGCATTCAAGGAA
ATATATGCAACGGGTAAAGGTTCATTTAAAATCAGGGCAAAGATATCAATTGATTACGCAGGTATCGGTCATACAAATCT
TGTCATAACTGAACTACCCTATATGGTTGGCTCTGAAAGGGTTATTGAAAAAATAAAACAGGGTGTAAATGCACACAGAA
TCGACGGAATTCTCGATGTCACTGATCTCAGTGACAGAAAAAACGGCTTAAAGCTTGTTATAGGAATTAAGCCGAACTTT
TCGCCAAATGAGGTAATGGATAACCTCTACCGGCATACCCCTCTTGAAGAATCGTTCAGTGTCAACAGTGTTGCCCTAAT
CGACGGAAAGCCTAAAACCCTTGGCATGCTGGATATGCTGAAGGTTTTTATAGACCACCGTGTATGTGTGATAACAAGGC
GCAGTAAGTTCTATTTGGCAACACACAAAAAGCGTCTTCATATGATCGATGGGCTTCTTTTGGCTCTAGTCGATATAGAT
AAAACAATCTCCATAATCCGAAACAGCGAAGGTATAAAAGAGGCTAGGAAAAACCTTATGAAGTGCCTGAATGTAACACG
GGATCAGGCAGAATACATCTTGGATATAAGACTGAATATGCTAACCAAACTCTCGACTATAAAACTTCAGTCTGAGCAGG
AGGAACTTAGAAAAACCACGCAAGACCTTGAAGAAATTCTTTCTGACCCGAACAAAATCGATGCGATTCTTATCGATGAA
CTAAAAAAGGTGGCACAAAGACTCCCATCACCCCGTAGAACGATTTTAGAGTCAAGTGTCAAAAGTAAAAAACAAAAGAC
GGATTATCCTCGTCAAGTTGTTTTAACCACCAATGGCTTTATATTCACAATTGAGGTGAACAATGATTTGATATCAGAAT
TGAGCCAACCCGAGACGTTTGACATGACATTCAATACCCACAGCGAACCTTTGCGTGTTGTGAAATCTTCCATTGTTTGT
GGGGTGAGATCAAGAATTGGCGCTCTGACCAGTGCGGGAAAGGTCATACCATTCACTCCAATCGGTCTTCCGGTATTGGA
CAGTGGATTTGTCACTGCAAAGAGTTTCCTCGGACTTGAAAATGACGAAGAAGCTGTTTTGCTTTTCGAGCTTCACCAGA
AAGAAAGTCTTGCCATTGCCACATCAAGTGGAATTGTGAAGCGTGTAACTCCGGCTATATGGCCAGATAGAACTGAGCGG
GTAATTACTCTCAGTGCGGGTGACAGGGTTGTTGGGGCCGCTAGAGGTGTATCCAGTTGTGGGTACTTTGTTTTCATAAC
TGCAAAAGGCAATCTCTTGCACTACAGTGCAGGCCTTGTTAACCCCCAAAATCGGCCCGCAAGAGGGGTTGCAGGTGTAA
CCCTAAAGGATGATGACGACAGGGTTATCAGTTTCGGCTATACAGAAGATGTAGATAGGGCAAATGTACTGACTGTATCA
AAAAGAATGACGACATCACGCCTTAGCTCTTATCAAACAAGAGGTAGGGCAACAAGAGGTGTGCAATGTCACAAGTTTCT
AAAAGGCGAAGAGTGGTTACACGCAGCAATTGTTGGAGAGAATTTGGCAGCAATAGACAATTTGGGCAATGTCGTAGCCA
TTGGCAAAAGCAGTGTGACTGCAAAAAGTTGTGCAGGAGTGAAACAATATCGATTGCCACAAGAGAAATCGGGAAAAAGT
AAGCGAGAAGACCGACTAATTAGATATCTCGGATATCCTTTCGTGCACGGCAATGACACCTATTAG

Upstream 100 bases:

>100_bases
CAGCTGACCCTGAAAGGGCTCGTCAGACATCGAGGCGTACTAGCGTTCCGAGCTGGGATGAAATCCTTTTCGGTTCTGAC
AAAAACAAGAATTGAGTGGC

Downstream 100 bases:

>100_bases
CAATGACACCTATTAATGGCAAACCGCTGACGGACCTTACAACGTGACTAGGGGCCGCATTGCATTCAGCTCTAATATGG
CCCACAGAGGGCGAGTATGT

Product: DNA topoisomerase IV subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 821; Mature: 821

Protein sequence:

>821_residues
MDKDIPEIFDVISDNEKITDVDLLHQMQDSFLEYAYSVIHSRALPDARDGLKPVQRRILYTMSRMGLSPDKAHVKCARVT
GEVMGKLHPHGDAAIYDTLVRMSQDFTMRIPLIDGHGNFGSLNDGPAASRYTEARLSRAAVAMINDLESNVVDFVPNYDN
QFTQPEVLPALLPNLIVNGSSGIAVGMATSIPPHNLQETVRAAIYLVRNRQSSLEQIMKILPGPDFPTGSIIVGKEAFKE
IYATGKGSFKIRAKISIDYAGIGHTNLVITELPYMVGSERVIEKIKQGVNAHRIDGILDVTDLSDRKNGLKLVIGIKPNF
SPNEVMDNLYRHTPLEESFSVNSVALIDGKPKTLGMLDMLKVFIDHRVCVITRRSKFYLATHKKRLHMIDGLLLALVDID
KTISIIRNSEGIKEARKNLMKCLNVTRDQAEYILDIRLNMLTKLSTIKLQSEQEELRKTTQDLEEILSDPNKIDAILIDE
LKKVAQRLPSPRRTILESSVKSKKQKTDYPRQVVLTTNGFIFTIEVNNDLISELSQPETFDMTFNTHSEPLRVVKSSIVC
GVRSRIGALTSAGKVIPFTPIGLPVLDSGFVTAKSFLGLENDEEAVLLFELHQKESLAIATSSGIVKRVTPAIWPDRTER
VITLSAGDRVVGAARGVSSCGYFVFITAKGNLLHYSAGLVNPQNRPARGVAGVTLKDDDDRVISFGYTEDVDRANVLTVS
KRMTTSRLSSYQTRGRATRGVQCHKFLKGEEWLHAAIVGENLAAIDNLGNVVAIGKSSVTAKSCAGVKQYRLPQEKSGKS
KREDRLIRYLGYPFVHGNDTY

Sequences:

>Translated_821_residues
MDKDIPEIFDVISDNEKITDVDLLHQMQDSFLEYAYSVIHSRALPDARDGLKPVQRRILYTMSRMGLSPDKAHVKCARVT
GEVMGKLHPHGDAAIYDTLVRMSQDFTMRIPLIDGHGNFGSLNDGPAASRYTEARLSRAAVAMINDLESNVVDFVPNYDN
QFTQPEVLPALLPNLIVNGSSGIAVGMATSIPPHNLQETVRAAIYLVRNRQSSLEQIMKILPGPDFPTGSIIVGKEAFKE
IYATGKGSFKIRAKISIDYAGIGHTNLVITELPYMVGSERVIEKIKQGVNAHRIDGILDVTDLSDRKNGLKLVIGIKPNF
SPNEVMDNLYRHTPLEESFSVNSVALIDGKPKTLGMLDMLKVFIDHRVCVITRRSKFYLATHKKRLHMIDGLLLALVDID
KTISIIRNSEGIKEARKNLMKCLNVTRDQAEYILDIRLNMLTKLSTIKLQSEQEELRKTTQDLEEILSDPNKIDAILIDE
LKKVAQRLPSPRRTILESSVKSKKQKTDYPRQVVLTTNGFIFTIEVNNDLISELSQPETFDMTFNTHSEPLRVVKSSIVC
GVRSRIGALTSAGKVIPFTPIGLPVLDSGFVTAKSFLGLENDEEAVLLFELHQKESLAIATSSGIVKRVTPAIWPDRTER
VITLSAGDRVVGAARGVSSCGYFVFITAKGNLLHYSAGLVNPQNRPARGVAGVTLKDDDDRVISFGYTEDVDRANVLTVS
KRMTTSRLSSYQTRGRATRGVQCHKFLKGEEWLHAAIVGENLAAIDNLGNVVAIGKSSVTAKSCAGVKQYRLPQEKSGKS
KREDRLIRYLGYPFVHGNDTY
>Mature_821_residues
MDKDIPEIFDVISDNEKITDVDLLHQMQDSFLEYAYSVIHSRALPDARDGLKPVQRRILYTMSRMGLSPDKAHVKCARVT
GEVMGKLHPHGDAAIYDTLVRMSQDFTMRIPLIDGHGNFGSLNDGPAASRYTEARLSRAAVAMINDLESNVVDFVPNYDN
QFTQPEVLPALLPNLIVNGSSGIAVGMATSIPPHNLQETVRAAIYLVRNRQSSLEQIMKILPGPDFPTGSIIVGKEAFKE
IYATGKGSFKIRAKISIDYAGIGHTNLVITELPYMVGSERVIEKIKQGVNAHRIDGILDVTDLSDRKNGLKLVIGIKPNF
SPNEVMDNLYRHTPLEESFSVNSVALIDGKPKTLGMLDMLKVFIDHRVCVITRRSKFYLATHKKRLHMIDGLLLALVDID
KTISIIRNSEGIKEARKNLMKCLNVTRDQAEYILDIRLNMLTKLSTIKLQSEQEELRKTTQDLEEILSDPNKIDAILIDE
LKKVAQRLPSPRRTILESSVKSKKQKTDYPRQVVLTTNGFIFTIEVNNDLISELSQPETFDMTFNTHSEPLRVVKSSIVC
GVRSRIGALTSAGKVIPFTPIGLPVLDSGFVTAKSFLGLENDEEAVLLFELHQKESLAIATSSGIVKRVTPAIWPDRTER
VITLSAGDRVVGAARGVSSCGYFVFITAKGNLLHYSAGLVNPQNRPARGVAGVTLKDDDDRVISFGYTEDVDRANVLTVS
KRMTTSRLSSYQTRGRATRGVQCHKFLKGEEWLHAAIVGENLAAIDNLGNVVAIGKSSVTAKSCAGVKQYRLPQEKSGKS
KREDRLIRYLGYPFVHGNDTY

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=362, Percent_Identity=25.9668508287293, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI19913406, Length=358, Percent_Identity=25.1396648044693, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788562, Length=778, Percent_Identity=34.8329048843188, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1789396, Length=509, Percent_Identity=38.8998035363458, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535065, Length=257, Percent_Identity=26.8482490272374, Blast_Score=84, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI212645657, Length=266, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI212645845, Length=165, Percent_Identity=29.0909090909091, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115534553, Length=164, Percent_Identity=29.2682926829268, Blast_Score=70, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=284, Percent_Identity=26.056338028169, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI17136538, Length=361, Percent_Identity=24.3767313019391, Blast_Score=79, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 91039; Mature: 91039

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKDIPEIFDVISDNEKITDVDLLHQMQDSFLEYAYSVIHSRALPDARDGLKPVQRRILY
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHH
TMSRMGLSPDKAHVKCARVTGEVMGKLHPHGDAAIYDTLVRMSQDFTMRIPLIDGHGNFG
HHHHCCCCCCHHHEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCC
SLNDGPAASRYTEARLSRAAVAMINDLESNVVDFVPNYDNQFTQPEVLPALLPNLIVNGS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCC
SGIAVGMATSIPPHNLQETVRAAIYLVRNRQSSLEQIMKILPGPDFPTGSIIVGKEAFKE
CCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEECHHHHHH
IYATGKGSFKIRAKISIDYAGIGHTNLVITELPYMVGSERVIEKIKQGVNAHRIDGILDV
HHHCCCCCEEEEEEEEEEEECCCCCCEEEEECCHHCCCHHHHHHHHCCCCCCCCCCEEEE
TDLSDRKNGLKLVIGIKPNFSPNEVMDNLYRHTPLEESFSVNSVALIDGKPKTLGMLDML
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHH
KVFIDHRVCVITRRSKFYLATHKKRLHMIDGLLLALVDIDKTISIIRNSEGIKEARKNLM
HHHHCCCEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KCLNVTRDQAEYILDIRLNMLTKLSTIKLQSEQEELRKTTQDLEEILSDPNKIDAILIDE
HHHHCCHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LKKVAQRLPSPRRTILESSVKSKKQKTDYPRQVVLTTNGFIFTIEVNNDLISELSQPETF
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCHHHHHCCCCCEE
DMTFNTHSEPLRVVKSSIVCGVRSRIGALTSAGKVIPFTPIGLPVLDSGFVTAKSFLGLE
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEECCCCHHHHHHCCCC
NDEEAVLLFELHQKESLAIATSSGIVKRVTPAIWPDRTERVITLSAGDRVVGAARGVSSC
CCCCEEEEEEECCCCCEEEEECCCCHHHCCCCCCCCCCCEEEEEECCCCEEEHHCCCCCC
GYFVFITAKGNLLHYSAGLVNPQNRPARGVAGVTLKDDDDRVISFGYTEDVDRANVLTVS
CEEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEH
KRMTTSRLSSYQTRGRATRGVQCHKFLKGEEWLHAAIVGENLAAIDNLGNVVAIGKSSVT
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHEEEEHHCCCHHHHHCCCCEEEECCCCCC
AKSCAGVKQYRLPQEKSGKSKREDRLIRYLGYPFVHGNDTY
HHHHCCHHHHCCCCCCCCCCHHHHHHHHHHCCCEECCCCCC
>Mature Secondary Structure
MDKDIPEIFDVISDNEKITDVDLLHQMQDSFLEYAYSVIHSRALPDARDGLKPVQRRILY
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHH
TMSRMGLSPDKAHVKCARVTGEVMGKLHPHGDAAIYDTLVRMSQDFTMRIPLIDGHGNFG
HHHHCCCCCCHHHEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCC
SLNDGPAASRYTEARLSRAAVAMINDLESNVVDFVPNYDNQFTQPEVLPALLPNLIVNGS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCC
SGIAVGMATSIPPHNLQETVRAAIYLVRNRQSSLEQIMKILPGPDFPTGSIIVGKEAFKE
CCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEECHHHHHH
IYATGKGSFKIRAKISIDYAGIGHTNLVITELPYMVGSERVIEKIKQGVNAHRIDGILDV
HHHCCCCCEEEEEEEEEEEECCCCCCEEEEECCHHCCCHHHHHHHHCCCCCCCCCCEEEE
TDLSDRKNGLKLVIGIKPNFSPNEVMDNLYRHTPLEESFSVNSVALIDGKPKTLGMLDML
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHH
KVFIDHRVCVITRRSKFYLATHKKRLHMIDGLLLALVDIDKTISIIRNSEGIKEARKNLM
HHHHCCCEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KCLNVTRDQAEYILDIRLNMLTKLSTIKLQSEQEELRKTTQDLEEILSDPNKIDAILIDE
HHHHCCHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LKKVAQRLPSPRRTILESSVKSKKQKTDYPRQVVLTTNGFIFTIEVNNDLISELSQPETF
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCHHHHHCCCCCEE
DMTFNTHSEPLRVVKSSIVCGVRSRIGALTSAGKVIPFTPIGLPVLDSGFVTAKSFLGLE
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEECCCCHHHHHHCCCC
NDEEAVLLFELHQKESLAIATSSGIVKRVTPAIWPDRTERVITLSAGDRVVGAARGVSSC
CCCCEEEEEEECCCCCEEEEECCCCHHHCCCCCCCCCCCEEEEEECCCCEEEHHCCCCCC
GYFVFITAKGNLLHYSAGLVNPQNRPARGVAGVTLKDDDDRVISFGYTEDVDRANVLTVS
CEEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEH
KRMTTSRLSSYQTRGRATRGVQCHKFLKGEEWLHAAIVGENLAAIDNLGNVVAIGKSSVT
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHEEEEHHCCCHHHHHCCCCEEEECCCCCC
AKSCAGVKQYRLPQEKSGKSKREDRLIRYLGYPFVHGNDTY
HHHHCCHHHHCCCCCCCCCCHHHHHHHHHHCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]