Click here to switch to the map view.

The map label for this gene is ispE

Identifier: 28572322

GI number: 28572322

Start: 187878

End: 189107

Strand: Reverse

Name: ispE

Synonym: TW159

Alternate gene names: 28572322

Gene position: 189107-187878 (Counterclockwise)

Preceding gene: 28572325

Following gene: 28572319

Centisome position: 20.42

GC content: 46.5

Gene sequence:

>1230_bases
ATGAAAACCGATGGTGGTAATACTTGGCGCGCCAGCCATAGCAAGCCACTAAATACTGCAAATACTATGGGGGAACCTTT
TAGTCATTCTGAATATTCAGTGCATGCGGATCAATCTGAATTCTATCTAAATGAATTGACAGAACATTCTGGTCAGGATA
ATCCCTGTATGAACACTAGCAGGCTGAATACTAACAGATATGGCCATCCTGTTGTGCATCCATGTCCCAAAATACACTGC
ATTTGCACACAGTCAAATATCGCTGCTATTGGGTCAGATTGTACGGGGTGTGTGGATATTGCCCAGGCGTGCAAAATGCT
GCGAGGTGGGCAGGGGTGCACACAAGACCCATGCGTAAAAAACCCTCACACACAATGTTTTACGGACGTGTCAAACCATG
CTATGCGTAATGTTTTACCGCTGAATGTATCAAATACAGAGCAATTCCCGATACAAATAGAATACGCAAACGGCCGAAAT
CCCGTCTTGAATCCAATGGATGATCTCGCGATGCGTGCAGCCCTTTTACTATCTAAGGATATAGATCTTCAAAATACACA
TATTTTACCCAGTACGCGTATTTCGATTGAGAAGAATATTCCGGTTGCCGCTGGTCTGGCGGGGGGTTCAGCTGACGCTG
CAGCGGTCTTGCTTGGGATAAACAGTGCATGGCAAACAAATTATTCTAGATGTGATTTGCTAGGAAAGGCAGGTGCACTC
GGTGCAGATGTACCCTTTTTAATCTGGGGTGGGGCTGCCTATGGAAGCGGAACCGGTTCATGTGTGACTTTTTTTGAAAC
ACAAACTCTGTACTGGGTTTTATGCTTTTCAAAGCATCCTCTATCCACTCGCAAGGTATTTCAAGAGCTCGATCGACAGC
GCAGTGGTGCCGGATGCAATCATCACCCTGTTTTTTCCAACCCAGCAGAGTGTGCAGAAATGCTAAAAAAGGCAATAAAA
AGAGGGCCAGAAGCGCTAGCAGCTCTTTTGCATAATGATCTTACAAGCGCCGCAAAGATGCTTATGCCAGAAATTGCAGA
GCGGATCAAAGCAGCAGAAAGATGCCCTGGGATTCTGCGAGCAATCATAAGCGGATCCGGACCGACCCTTGCCCTGTTGG
CAGAAGACGCAGAGGCGGCTAATAGGGCATGCAGTATCCTGAAAGACACGGGTGTTATCTGCAAAGCAGTATCATCTCCG
GCGTATTCGAGTATATACTGGCAGACGTAA

Upstream 100 bases:

>100_bases
ACCAGATGGGTATCATGGGCTTTTTACCGTATATCTTGGGATATCGCTTTATGAGCACATAAACATTACGCTTGATGATT
CTTACAATAGGTCCCGCGAG

Downstream 100 bases:

>100_bases
TAATCCGCTTTAGTGTAACGGCAGCACAGCAGCCTTTGGAGCTGTTGGTCTAGGTTCGAATCCTAGAGGCGGAGCGCTTC
GTGTTTCTCCTTACGTGTTT

Product: 4-diphosphocytidyl-2-c-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKTDGGNTWRASHSKPLNTANTMGEPFSHSEYSVHADQSEFYLNELTEHSGQDNPCMNTSRLNTNRYGHPVVHPCPKIHC
ICTQSNIAAIGSDCTGCVDIAQACKMLRGGQGCTQDPCVKNPHTQCFTDVSNHAMRNVLPLNVSNTEQFPIQIEYANGRN
PVLNPMDDLAMRAALLLSKDIDLQNTHILPSTRISIEKNIPVAAGLAGGSADAAAVLLGINSAWQTNYSRCDLLGKAGAL
GADVPFLIWGGAAYGSGTGSCVTFFETQTLYWVLCFSKHPLSTRKVFQELDRQRSGAGCNHHPVFSNPAECAEMLKKAIK
RGPEALAALLHNDLTSAAKMLMPEIAERIKAAERCPGILRAIISGSGPTLALLAEDAEAANRACSILKDTGVICKAVSSP
AYSSIYWQT

Sequences:

>Translated_409_residues
MKTDGGNTWRASHSKPLNTANTMGEPFSHSEYSVHADQSEFYLNELTEHSGQDNPCMNTSRLNTNRYGHPVVHPCPKIHC
ICTQSNIAAIGSDCTGCVDIAQACKMLRGGQGCTQDPCVKNPHTQCFTDVSNHAMRNVLPLNVSNTEQFPIQIEYANGRN
PVLNPMDDLAMRAALLLSKDIDLQNTHILPSTRISIEKNIPVAAGLAGGSADAAAVLLGINSAWQTNYSRCDLLGKAGAL
GADVPFLIWGGAAYGSGTGSCVTFFETQTLYWVLCFSKHPLSTRKVFQELDRQRSGAGCNHHPVFSNPAECAEMLKKAIK
RGPEALAALLHNDLTSAAKMLMPEIAERIKAAERCPGILRAIISGSGPTLALLAEDAEAANRACSILKDTGVICKAVSSP
AYSSIYWQT
>Mature_409_residues
MKTDGGNTWRASHSKPLNTANTMGEPFSHSEYSVHADQSEFYLNELTEHSGQDNPCMNTSRLNTNRYGHPVVHPCPKIHC
ICTQSNIAAIGSDCTGCVDIAQACKMLRGGQGCTQDPCVKNPHTQCFTDVSNHAMRNVLPLNVSNTEQFPIQIEYANGRN
PVLNPMDDLAMRAALLLSKDIDLQNTHILPSTRISIEKNIPVAAGLAGGSADAAAVLLGINSAWQTNYSRCDLLGKAGAL
GADVPFLIWGGAAYGSGTGSCVTFFETQTLYWVLCFSKHPLSTRKVFQELDRQRSGAGCNHHPVFSNPAECAEMLKKAIK
RGPEALAALLHNDLTSAAKMLMPEIAERIKAAERCPGILRAIISGSGPTLALLAEDAEAANRACSILKDTGVICKAVSSP
AYSSIYWQT

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_TROW8 (Q83IA0)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789102.1
- ProteinModelPortal:   Q83IA0
- SMR:   Q83IA0
- STRING:   Q83IA0
- GeneID:   1064454
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW159
- eggNOG:   COG1947
- HOGENOM:   HBG251166
- OMA:   QNTHILP
- ProtClustDB:   CLSK229523
- BioCyc:   TWHI218496:TW0147-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 43972; Mature: 43972

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: ACT_SITE 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDGGNTWRASHSKPLNTANTMGEPFSHSEYSVHADQSEFYLNELTEHSGQDNPCMNTS
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCC
RLNTNRYGHPVVHPCPKIHCICTQSNIAAIGSDCTGCVDIAQACKMLRGGQGCTQDPCVK
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
NPHTQCFTDVSNHAMRNVLPLNVSNTEQFPIQIEYANGRNPVLNPMDDLAMRAALLLSKD
CCCHHHHHHHHHHHHHCEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
IDLQNTHILPSTRISIEKNIPVAAGLAGGSADAAAVLLGINSAWQTNYSRCDLLGKAGAL
CCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCEEEEEECCHHHHCCCHHHHHHHCCCCC
GADVPFLIWGGAAYGSGTGSCVTFFETQTLYWVLCFSKHPLSTRKVFQELDRQRSGAGCN
CCCCCEEEECCCCCCCCCCCEEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
HHPVFSNPAECAEMLKKAIKRGPEALAALLHNDLTSAAKMLMPEIAERIKAAERCPGILR
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
AIISGSGPTLALLAEDAEAANRACSILKDTGVICKAVSSPAYSSIYWQT
HHHCCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKTDGGNTWRASHSKPLNTANTMGEPFSHSEYSVHADQSEFYLNELTEHSGQDNPCMNTS
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCC
RLNTNRYGHPVVHPCPKIHCICTQSNIAAIGSDCTGCVDIAQACKMLRGGQGCTQDPCVK
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
NPHTQCFTDVSNHAMRNVLPLNVSNTEQFPIQIEYANGRNPVLNPMDDLAMRAALLLSKD
CCCHHHHHHHHHHHHHCEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
IDLQNTHILPSTRISIEKNIPVAAGLAGGSADAAAVLLGINSAWQTNYSRCDLLGKAGAL
CCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCEEEEEECCHHHHCCCHHHHHHHCCCCC
GADVPFLIWGGAAYGSGTGSCVTFFETQTLYWVLCFSKHPLSTRKVFQELDRQRSGAGCN
CCCCCEEEECCCCCCCCCCCEEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
HHPVFSNPAECAEMLKKAIKRGPEALAALLHNDLTSAAKMLMPEIAERIKAAERCPGILR
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
AIISGSGPTLALLAEDAEAANRACSILKDTGVICKAVSSPAYSSIYWQT
HHHCCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12606174