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The map label for this gene is manA [H]

Identifier: 28572296

GI number: 28572296

Start: 140623

End: 141990

Strand: Direct

Name: manA [H]

Synonym: TW133

Alternate gene names: 28572296

Gene position: 140623-141990 (Clockwise)

Preceding gene: 28572294

Following gene: 28572297

Centisome position: 15.19

GC content: 46.35

Gene sequence:

>1368_bases
ATGCGCAGGCCTGACATCTTCTTGCTGAAGAATGCACCTATGAATTACGATTGGGGCGATTGCACAGCTATAGCTAGTCT
TCAGGGAAGACAGCCCACATCACGCCCAGAAGCTGAGATCTGGTTCGGCACGCACCCCAGGTCACCTGCCTATCTTTCAG
ACGGTAGATTACTTGCTAGTGCTCTAGAAGAGCACGGAATCTCCATCTCGTATTTGGTGAAGCTGCTTGCCGCTGCAAAG
CCACTTTCAATTCAGGTTCATCCGAATTCTGCGCAGGCTCGCGCAGGATTCGCCAGAGAAAAGCATCTTGATTCTGCATC
CAAGAACTATAGGGACAGATATGAGAAGTCTGAGATGCTTATTGCGATAAGCGATACGTTCAGCCTATTGTGTGGATTCA
GGCCCAAGGATGAGTTGTCCGAGACATTAAACCATTTCTCTCTTGTTTCAAAAAAATTCAAAATCATGAGGGATCTTTAC
GAACAACACGGATTGAAATGCGCTATTGCTTGGTTTTTTGAAAATGCCAACCAGAGTGATATCGATTTATTGTGCGAAGG
AAGAATGCCGTATGAATCACAGAACACCACAATATCCGACCTAAAGAAACACCATGTCAATGAACCTGGATTTGTTTTGG
CGGTCCTCATGAGTCGTGTCAATATCACTCGCGGACAGTCGGTTTTTGTACAAGCCGGCACCCTGCATTCGTACCTCAAA
GGCTTTGGGGTCGAGATTATGACAAATAGTGACAATGTGATTCGCGCAGGGCTAACAACAAAACATATTGACACAGTCGA
GTTACAAAAGGTGGCCCTGTTTAAGTCAGAACCAGTTTGTCTTCTGCAGCCACACATCAGTAATTCAGGGGCCAGTAGGC
GTTTTTGCACGCCATACTTTACAGTTGAGGAGGTTACGCTCTCTGGTAATGAGGGCAGTTGCCTTAGCTCTACTATCGGC
AATTGTGCGTACTGTAACAGAGATAGCGATTCAGACCTTACATCTGTGCTGCAACATGATTTGGATCAAAAACATGATGG
CATGACAGAACCTTCACCAGGACTTTCCGAGGGTATTCATGCATGTAATTCAGGTATCACTTGTACTTCTGAATCCATCT
CTAAGTCGGCCACGGCCATCCCTGGTGGCACCTTCGGTGGATCAGGATACACCTTACGGGGTACTGGACTTGTGCTTGTC
ACAGCTGGTGTGGTTCATTTATCATCAGGTGACGAAGAATTGATAGTACGCTCCGGATTTGCCGCGTTTGTAATCGACCC
GTCGCCATCTGGCGGTGTTTTTCTCACAGGGTGCGGTGTTTGTTATATCGTTACTGATGATAATCAGTTTGACATAATTG
CTTCTTAG

Upstream 100 bases:

>100_bases
ATTCAACACACATATTCGACACATATCCGTGCATGCCATATCGCGATTCCAATTGTGTCTATATGGCTTTGGCAGCTCAA
GCATCATATACTCTACTGTT

Downstream 100 bases:

>100_bases
CTCGTATCATTAGTGAGTTACAGATGTGTAATTTTACAGATTGGGGTGAGAGTGTCAGATAACTTGCCGATACCGGATGA
CTGGCATATTGACCCTGTTT

Product: mannose-6-phosphate isomerase

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MRRPDIFLLKNAPMNYDWGDCTAIASLQGRQPTSRPEAEIWFGTHPRSPAYLSDGRLLASALEEHGISISYLVKLLAAAK
PLSIQVHPNSAQARAGFAREKHLDSASKNYRDRYEKSEMLIAISDTFSLLCGFRPKDELSETLNHFSLVSKKFKIMRDLY
EQHGLKCAIAWFFENANQSDIDLLCEGRMPYESQNTTISDLKKHHVNEPGFVLAVLMSRVNITRGQSVFVQAGTLHSYLK
GFGVEIMTNSDNVIRAGLTTKHIDTVELQKVALFKSEPVCLLQPHISNSGASRRFCTPYFTVEEVTLSGNEGSCLSSTIG
NCAYCNRDSDSDLTSVLQHDLDQKHDGMTEPSPGLSEGIHACNSGITCTSESISKSATAIPGGTFGGSGYTLRGTGLVLV
TAGVVHLSSGDEELIVRSGFAAFVIDPSPSGGVFLTGCGVCYIVTDDNQFDIIAS

Sequences:

>Translated_455_residues
MRRPDIFLLKNAPMNYDWGDCTAIASLQGRQPTSRPEAEIWFGTHPRSPAYLSDGRLLASALEEHGISISYLVKLLAAAK
PLSIQVHPNSAQARAGFAREKHLDSASKNYRDRYEKSEMLIAISDTFSLLCGFRPKDELSETLNHFSLVSKKFKIMRDLY
EQHGLKCAIAWFFENANQSDIDLLCEGRMPYESQNTTISDLKKHHVNEPGFVLAVLMSRVNITRGQSVFVQAGTLHSYLK
GFGVEIMTNSDNVIRAGLTTKHIDTVELQKVALFKSEPVCLLQPHISNSGASRRFCTPYFTVEEVTLSGNEGSCLSSTIG
NCAYCNRDSDSDLTSVLQHDLDQKHDGMTEPSPGLSEGIHACNSGITCTSESISKSATAIPGGTFGGSGYTLRGTGLVLV
TAGVVHLSSGDEELIVRSGFAAFVIDPSPSGGVFLTGCGVCYIVTDDNQFDIIAS
>Mature_455_residues
MRRPDIFLLKNAPMNYDWGDCTAIASLQGRQPTSRPEAEIWFGTHPRSPAYLSDGRLLASALEEHGISISYLVKLLAAAK
PLSIQVHPNSAQARAGFAREKHLDSASKNYRDRYEKSEMLIAISDTFSLLCGFRPKDELSETLNHFSLVSKKFKIMRDLY
EQHGLKCAIAWFFENANQSDIDLLCEGRMPYESQNTTISDLKKHHVNEPGFVLAVLMSRVNITRGQSVFVQAGTLHSYLK
GFGVEIMTNSDNVIRAGLTTKHIDTVELQKVALFKSEPVCLLQPHISNSGASRRFCTPYFTVEEVTLSGNEGSCLSSTIG
NCAYCNRDSDSDLTSVLQHDLDQKHDGMTEPSPGLSEGIHACNSGITCTSESISKSATAIPGGTFGGSGYTLRGTGLVLV
TAGVVHLSSGDEELIVRSGFAAFVIDPSPSGGVFLTGCGVCYIVTDDNQFDIIAS

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide [H]

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family [H]

Homologues:

Organism=Homo sapiens, GI4505235, Length=315, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1787899, Length=339, Percent_Identity=30.6784660766962, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71997620, Length=308, Percent_Identity=30.1948051948052, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6320839, Length=387, Percent_Identity=26.6149870801034, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356061, Length=294, Percent_Identity=30.2721088435374, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01238 PMI_typeI [H]

EC number: =5.3.1.8 [H]

Molecular weight: Translated: 49534; Mature: 49534

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPDIFLLKNAPMNYDWGDCTAIASLQGRQPTSRPEAEIWFGTHPRSPAYLSDGRLLAS
CCCCCEEEEECCCCCCCCCCHHEEEECCCCCCCCCCCCEEEECCCCCCCCEECCCHHHHH
ALEEHGISISYLVKLLAAAKPLSIQVHPNSAQARAGFAREKHLDSASKNYRDRYEKSEML
HHHHHCCCHHHHHHHHHCCCCCEEEECCCCCHHHHCCHHHHHCCHHHHHHHHHHCCCCEE
IAISDTFSLLCGFRPKDELSETLNHFSLVSKKFKIMRDLYEQHGLKCAIAWFFENANQSD
EEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
IDLLCEGRMPYESQNTTISDLKKHHVNEPGFVLAVLMSRVNITRGQSVFVQAGTLHSYLK
EEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHH
GFGVEIMTNSDNVIRAGLTTKHIDTVELQKVALFKSEPVCLLQPHISNSGASRRFCTPYF
HCCEEEEECCCCEEEECCCCCCCCHHHHHEEEEECCCCEEEEECCCCCCCCCCCEECCEE
TVEEVTLSGNEGSCLSSTIGNCAYCNRDSDSDLTSVLQHDLDQKHDGMTEPSPGLSEGIH
EEEEEEEECCCCCHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ACNSGITCTSESISKSATAIPGGTFGGSGYTLRGTGLVLVTAGVVHLSSGDEELIVRSGF
HHCCCCEECCHHHCCCCCCCCCCCCCCCCEEEECCCEEEEEEEEEEECCCCCEEEEECCC
AAFVIDPSPSGGVFLTGCGVCYIVTDDNQFDIIAS
EEEEECCCCCCCEEEEECCEEEEEECCCCEEEEEC
>Mature Secondary Structure
MRRPDIFLLKNAPMNYDWGDCTAIASLQGRQPTSRPEAEIWFGTHPRSPAYLSDGRLLAS
CCCCCEEEEECCCCCCCCCCHHEEEECCCCCCCCCCCCEEEECCCCCCCCEECCCHHHHH
ALEEHGISISYLVKLLAAAKPLSIQVHPNSAQARAGFAREKHLDSASKNYRDRYEKSEML
HHHHHCCCHHHHHHHHHCCCCCEEEECCCCCHHHHCCHHHHHCCHHHHHHHHHHCCCCEE
IAISDTFSLLCGFRPKDELSETLNHFSLVSKKFKIMRDLYEQHGLKCAIAWFFENANQSD
EEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
IDLLCEGRMPYESQNTTISDLKKHHVNEPGFVLAVLMSRVNITRGQSVFVQAGTLHSYLK
EEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHH
GFGVEIMTNSDNVIRAGLTTKHIDTVELQKVALFKSEPVCLLQPHISNSGASRRFCTPYF
HCCEEEEECCCCEEEECCCCCCCCHHHHHEEEEECCCCEEEEECCCCCCCCCCCEECCEE
TVEEVTLSGNEGSCLSSTIGNCAYCNRDSDSDLTSVLQHDLDQKHDGMTEPSPGLSEGIH
EEEEEEEECCCCCHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ACNSGITCTSESISKSATAIPGGTFGGSGYTLRGTGLVLVTAGVVHLSSGDEELIVRSGF
HHCCCCEECCHHHCCCCCCCCCCCCCCCCEEEECCCEEEEEEEEEEECCCCCEEEEECCC
AAFVIDPSPSGGVFLTGCGVCYIVTDDNQFDIIAS
EEEEECCCCCCCEEEEECCEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6397402; 9097039; 9278503 [H]